View source for Opsin evolution: RBP3 (IRBP)

== RPB3 (IRBP): introduction ==

Interstitial retinol-binding protein, oddly named by IGNC as RBP3 despite a lack of paralogs RBP1 or RBP2, confusion with ribosomal binding protein genes and widespread prior use of protein name IRBP, is a 4 exon 1247 residue glycoprotein thought to shuttle retinoids interstitally between the photoreceptor cells and the retinal pigment epithelium. This role would only make sense for ciliary opsin systems that are unable to regenerate cis-retinal without an auxillary pathway in an anatomically separate tissue (here RPE). Consequently -- since nearly all protein folds are extremely ancient -- RPB3 must have been co-opted from some other role.

The protein's size results from four ancient internal tandem duplictions that became established prior to intronation (that is, the gene structure does not reflect the repeat structure; the repeats happened first, introns were inserted randomly later within the fourth repeat).  Any given repeat module clusters markedly better to the same-numbered repeat in other species than to any of the internal repeats, establishing that repeats had arisen and diverged already prior to speciation rather than arising independently in descendent lineages (like RHO1 and RHO2 in lamprey).

It was initially expected that IRBP as a self-contained homotetramer would load four molecules of trans-retinol to accomplish its passive shuttling efficiently. However experiments decisively show this to be false -- the subunits are quite inequivalent and only one molecule is transported. The structure of module 2 of frog was [determined in 2002 (PDB: 1J7X); it consists of two sub-domains and a cleft. The fold matches obscure proteases and hydrolases found in little-studied bacteria; this might represent convergent evolution as seen in TIM beta barrels rather than provide valid clues to ancestral function. A structural determination of the entire molecule is reportedly underway.

Fragments of the protein have been sequenced from an immense number of species for phylogenetic purposes. That's because it evolves fast enough to provide a large number of seemingly informative sites and these can be obtained conveniently exploiting the large size of the first reading frame. While there is minimal risk of accidentally cross-matching different modules, the internal repeat structure implies that residues might not be evolving independently. While the modules might seem too diverged for cross-module gene conversion to still be operative, they retain patches of near identity. 

Some very odd aspects of marsupial RBP3 are investigated below because of the implications in a gene so widely used in taxonomy. Teleost fish further raise the question of inhomogeneous recombination events arising from module mix-up. In fish, the ancestral four-module gene has [http://www.molvis.org/molvis/v12/a180/ given rise] to a two-module gene (M1 and M4) accompanied by an intronless upstream tandem fragment duplicate of M1-M3 without any indication of M4 in the residual 10 residues. Both genes are transcribed and apparently functional since the establishment event occured prior to zebrafish and fugu divergence, though the upstream gene has been lost in many lineages including tetraodon, medaka, and stickleback but not cichlids.

The upstream fragment suggests a truncated version of the first large exon. The lack of intron does not suggest retroprocessing in view of their lack in the parental gene and the locational adjacency but rather standard recombinational or flanking retroposon-driven tandem duplicating. The loss of M2 and M3 in the downstream gene but retention of standard introns again suggests recombinant loss, either as part of the initial tandem event or subsequent to it as a consequence of the new potential for exact misalignment. Note otherwise the four modules would have been quite diverged from each other prior to the emergence of rayfinned fish. 

Conceivably whole genome or segmental duplication in fish set the stage for inhomogeneous recombination (as [http://www.molvis.org/molvis/v12/a180/nickerson-fig10.html modelled] by Nickerson), though simple local tandem duplication event mediated by retroposons during replication is another option. A great many tandem loci exist in genomes such as human for which wholesale duplication is inapplicable. This could possibly be resolved by sequencing basal teleost fish that diverged prior to the putative genome duplication to determine if the odd arrangement of genes was already established. 

=== IRBP module alignment, conservation and structural aspects ===
 
The image below aligns the four module types from human to lamprey and amphioxus, with yellow showing exceptionally conserved residues and cyan moderately conserved. The number of species shown could be greatly expanded especially for module 1 but for clarity only a phylogenetically representative set is displayed. Module boundaries vary slightly according to the bioinformatics tool used to define the domain (eg cystallographic homology transfer, SuperFamily, Pfam, SwissProt, blastp of reference domains) but can be standardized by counting back a fixed number of residues N-terminally from the universally conserved tyrosine and forward from the C-terminal alanines because conserved residues must occupy near-identical positions within the tertiary structure.

It can be seen that M2 has two variable length insertional regions, no doubt relatively disordered loops. Amphioxus additionally has inserted residues in three regions that are suppressed in the figure -- observe that although its overall identity is low, identity is high in conserved residues. The modules each have distinctive class indels and characteristic residues that allow them to be readily distinguished from each other. These features were most likely established prior to lamprey divergence rather than later by convergence. Only two conserved patches are observed, GNvGLYRvD and RAivVGErT in human; other conserved residues are dispersed (at least within the linear structure).

[[Image:IRBPsstruc.png|left]]

The sole [http://tinyurl.com/cetu3a structural determination] to date, 1J7X_A the single module M2 of Xenopus laevis to 1.8 Å, describes two domains separated by a hydrophobic binding cleft (based on analogy to very similar protease and hydrolase folds). The smaller domain A is an an amino terminal three helix bundle that extends 8 residues past the M2 insertion region (to PGDSIQAEN in xenLae) . M2 was an unfortunate choice because it is most diverged and bares the least resemblance to early-diverging or ancestral sequence.

The observed fold similarities could not plausibly have arisen by chance. Crotonase has a binding site for hydrophobic chains of fatty acids and isomerizes them, with possible relevence in RBP3 to cis and trans retinoid stabilization and transport; however IRBP does not appear to be an enzyme or isomerase itself and RPE65 carries out the regenerative isomerization reaction. 

M2 may have two distinct binding sites, one in the cleft between domains A and B and the other solely inside domain B. The assignment of conserved tryptophans to these sites and other conserved residues to the surface (suggesting a protein binding partner, possibly intra-module binding) has to be revisited in view of the much deeper phylogenetic array of sequences now available.

A peculiar feature of full four-module protein (after discounting a couple dozen residues for signal peptide and carboxy terminal wander) is the cheek-to-jowl adjacency of consecutive modules -- in human at most 3 amino acids separate the modules from each other. 

This is far too short a bridge to allow many arrangement options for module-module quasi-homodimerization (or allosteric regulation of the binding subunit). Instead the modules must be arranged more as beads on a string. Linear donor-receptor docking in a M1-M2-M3-M4 arrangement risks endless polymerization unless the donor in M4 docks to the receptor in M1. However the bridge sequences are too short to permit closure into a tetrameric cycle.

Despite subunits quite diverged in sequence, a more likely arrangement is an anti-parallel dimer of M1-M2-M3-M4 to M4-M3-M2-M1 (as seen in [[Personal_genomics:_ACTN3#R577x_and_co-evolution_of_actinin_spectrin_repeats|actinin spectrin repeats]]). This arrangement allows each module to dimerize with the quasi dihedral symmetry, leaving no docking sites vacant. Because the binding patches are in paralogous modules, this explains why observed surface residue conservation in frog M2 carries over to the other modules. 

If true, this implies co-evolution of amino acids in these binding patches on top of what already occurs internally to an individual module. All phylogenetic software assumes -- ridiculously -- independent evolution of sites. However like morphological characters, molecular characters can have dependencies that amount to couplings and coordination between individual reduced alphabets. Here the much studied M1 module has surface residues co-evoving with counterparts on M4.

IRBP has only a single site with glycosylation potential (in [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=protein&dopt=GenPept&RID=V21ZK4W8012&log%24=protalign&blast_rank=1&list_uids=20150355 beta strand 8] of the hinge region of domain B at residues 204 and 515 which are paralogous under module alignment). Although the NxT motif occurs with excellent phylogenetic conservation depth (human to lamprey, also in fish fragmentary genes), only modules M1 and M2 exhibit it. Given the interstitial location and experimental support for glycoprotein nature, IRBP is very likely glycosylated. Bulky carbohydrate chains need to be out of the way of the putative dimerization patches.

[[Image:IRBPglycosyl.png]]

The situation with cysteines is not so clear. Here M1 has a cysteine at position 19 that is conserved back to lamprey (also in fish fragmentary genes) but A/T/S in other modules. A second conserved cysteine is found only in modules M2 and M3 at position 163 (human M1 numbering, residue is I). M4 has no conserved cysteines. A few other cysteines occur elsewhere but only sporadicaly in the comparative genomics sense. The cysteines at M2 and M3 coud potentially form a disulfide in the antiparallel dimer model discussed above. Inter-modular disulfides are not feasibly if modules are arranged linearly. No explanation is currently available for the conserved cysteine in M1.

An initial study of knockout mice disasterously used a strain carrying a [http://www.ncbi.nlm.nih.gov/pubmed/19074801 severe mutation] at L450 in [[Opsin_evolution:_RPE65|RPE65]], the enxyme catalyzing the rate-limiting isomerization step in the visual regeneration cycle. Lack of IRBP alone delays transfer of both bleached from photoreceptor cells and newly regenerated chromophore from RPE to photoreceptors.

A single known [http://www.ncbi.nlm.nih.gov/pubmed/19074801 disease mutation] D1080N causes a very rare recessive retinitis pigmentosa. This aspartate may disrupt an internal salt bridge according to the Xenopus M2 structure (and homology transfer to other modules]. It lies in M4 near the end of the second exon. This residue is conserved back to lamprey and amphioxus, indeed to crotonase and other weakly aligning homologs.

     *
 AIILDLRYNLGGDREGVVHWASFFF RBP3_braFlo
 A+I+D+R+N+GG    +    S+FF
 AMIIDMRFNIGGPTSSIPILCSYFF RBP3_homSap
  +IID+R+N+GG ++SIPILCSYFF
 ILIIDLRYNMGGYSTSIPILCSYFF RBP3_petMar
 
[[Image:IRBPconserved.png]]

 <font color="blue">M1_homSap  LFQPSLVLDMAKVLLDNYCFPENLLGMQEAIQQAIKSHEILSISDPQTLASVLTAGVQSSLNDPRLVISYEPST-----------PEPPPQVPALTSLSEEELLAWLQRGLRHEVLEGNVGYLRVDSVPGQEVLSMMGEFLVAHVWGNLMGTSALVLDL
 M1_bosTau  LFQPSLVLEMAQVLLDNYCFPENLMGMQGAIEQAIKSQEILSISDPQTLAHVLTAGVQSSLNDPRLVISYEPST-----------LEAPPRAPAVTNLTLEEIIAGLQDGLRHEILEGNVGYLRVDDIPGQEVMSKLRSFLVANVWRKLVNTSALVLDL
 M1_monDom  IFQPSLVRDMAKILLDNYCFPENLMGMQEVIEQAIKSGEILDISDPQMLASVLTAGVQGALNDPRLVISFEPSI-----------PETPQHVPKLANVTQEELLILLQQMIKYQVLEGNVGYLRVDYIPGQEVVEKVGEFLVNNIWKKLMGTSSLVLDL
 M1_macEug  IFQPSLVLDMAKILLDNYCFPENLMGMQEAIEQAIKSGEILDISDPQMLASVLTAGVQGSLNDPRLVISYEPSP-----------AEAPQQSPKLTSLTQEELLTLLQQMIKYQVLDGNVGYLRVDYIPGQEVVEKVGEFLVNDIWKKLMGTSSLVLDL
 M1_sacHar  IFQPTLVLDMAKILLDNYCFPENLMGMQEAIEQAIKSGEILDISDPQMLANVLTAGVQGSLNDPRLVISYEPST-----------SEAPQHDPKFANATQEELLALFQKIIKYQVLEDNVGYLRVDYIPGRDMIEEVGEFLVNDIWKKVMETSSLVLDL
 M1_ornAna  VSQPSMVLDVAKILLDNYCYPENLMGMQEAIEEAIQRGEILDIADPKRLASVLTAGVQGSLNDPRLVISYEPAP-----------VAVSQQPPEPASLPAEQPLERLRPAVGSEVLEGNVGYLRVDRLPGREEIERVGAVLGRDIWEKLLGTSALVLDL
 M1_galGal  IFQPTLVLDMAKVLLDNYCYPENLVGMQEAIEQAIKSGEILDISDPKMLANVLTAGVQGALNDPRLVISYEPSL-----------HAAPKQEAE-TYPTREQLLSLIEHVVIYDKLEGNVGYLRIDYIIGQEVVEKVGAFLVDKVWKTLINTSALVIDL
 M1_taeGut  IFQPTLVLDMAKVLLDNYCYPENLVGMQEAIEQAIKSGEILDISDPKMLANVLTAGVQGALNDPRLVISYEPLP-----------HSGPKQEAE-GSPTREQLLSLIEHVIMYDKLEGNVGYLRIDYIIGEEVVQKVGAFLVDKVWKTLIETSALVIDL
 M1_anoCar  VLQSTLVLDMAKLLLDNYCLPENLVGMREAIEQAIKNGEVLDISDPKLLATVLTAGVQGALNDPRLVISYEPTA-----------PAAPKQRME-TSLTPEQLLSLIQHTVKYEVLDDNVGYLRIDYIMGQDIVQKIGSFLVEKVWKTLLGTSALILDL
 M1_xenLae  LFQPSLVMDMAKVLLDNYCFPENLVGMQETIEQAVKGGEILHISDPDTLANVFTSGVQGYLNDPRLVVSYEPN-------------YSGPQTEQSLELTPEQLKFLINHSVKYDILPGNIGYLRIDFIIGQDVVQKVGPHLVNNIWKKLMPTSALILDL
 M1_xenTro  VFQPSLVMDMAKVLLDNYCFPENLVGMQETIEQAMKSGEILHISDPETLANVFTSGVQGFLNDPRLVVSYEPN-------------YSGPRKEQSPEPTLEQLKFLLDHSVTYDLLPGNIGYLRIDFIIGQDVVQKVGPLLVNNIWKKLMPSSALILDL
 M1_tetNig  AFPPSLIADMAKIVLDNYCSPEKLAGMKEAIKAAGTNTEVLNIPDGESLARVLSAGVQGTVSDPRLMVSFQPN-------------YVPAGPHKMPPLPPEHLVAVLQTSVKLDILEGNTGYLRIDHILGEEVADKVGPALIDLIWNKILPTSALIFDL
 M1_takRub  AFPPSLITDMAKIVLDNYCSPEKLAGMKEAIEAAGTNTEVLNIPDGESLARVLSAGVQGTVSDSRLMVSYQPD-------------YVPAVPPKMPPLPPEHLVAVLQTSIKLDLLEGNTGYLRIDHIIGEDVAEKVGPSLIDLIWNKILPTSALIFDL
 M1_gasAcu  GFAPNVIIDMAKIVIDNYCSPEKLAGMKEAIEAAGSNTEVLSIPDAETLANVLSAGVQTTVSDPRLMISYEPN-------------YVPVVPPKMPPLPPDQVIAVLQTSIKLDILEGNIGYLRIDHILGEDVAEKVGPLLLDLVWNKILPTSALIFDL
 M1_oryLat  SFPPSLITDLAKIVMDNYCSPEKLSGMKEDIATAGANTDVLNIPDGEALAKVLTDGVQTTVSDPRLRVSYEPN-------------YVPVVP---PQLPPEQLIAVLQTSIKLDILEGNIGYLRIDSIIGEEVAEKVGPLLLELVWSKILPTSALIFDL
 M1_danRer  NFSPTLIADMAKIFMDNYCSPEKLTGMEEAIDAASSNTEILSISDPTMLANVLTDGVKKTISDSRVKVTYEPD-------------LILAAPPAMPDIPLEHLAAMIKGTVKVEILEGNIGYLKIQHIIGEEMAQKVGPLLLEYIWDKILPTSAMILDF
 M1_petMar  KFDTAVVLHLAKVLLDNYCIPENLVGMDEAIQRAVDNGELLGVSDPESAASALTEGIQAALNDPRIAVSYVA-----------------------PPHTFEELLATIPQKTSFAVLDGNVGYLRADEIISEATIKKLGPVIVQRIWNRLVDTDTFVLDL</font>
 M1x_takRu  FYQHTLVLEMAKLLLENYCIPENLVGMQEAIQRAIKSREILQISDRKTLATVLTVGVQGALNDPRLSVSYEPS-------------FSPLPLQALSSLPVEQQLRLLRNSIKLDILDSDVGYLRIDRIIDEETLLKFGPLLRENVWDKAAQTSSLILDL
 M1x_danRe  SFQSALVLDMAKILLDNYCFPENLIGMQEAIQQAINSGEILHISDRKTLASVLTAGVQGALNDPRLTVSYEPN-------------YTLITPPALHSLPTEQLIRLIRSTVKLEVMDNNIGYLRIDRIIGQETVVKLGRLLHNNIWKKVAHTSAMIFDL
 <font color="red">M2_homSap  SALPGVVHCLQEVLKDYYTLVDRVPTLLQHLASM----DFSTVVSEEDLVTKLNAGLQAASEDPRLLVRAIGPTETPSWPAPDAAAEDSPGVAPELPEDEAIRQALVDSVFQVSVLPGNVGYLRFDSFADASVLGVLAPYVLRQVWEPLQDTEHLIMDL
 M2_bosTau  RALPGVIQRLQEALREYYTLVDRVPALLSHLAAM----DLSSVVSEDDLVTKLNAGLQAVSEDPRLQVQVVRPKEASS--GPEEEAEEPPEAVPEVPEDEAVRRALVDSVFQVSVLPGNVGYLRFDSFADASVLEVLGPYILHQVWEPLQDTEHLIMDL
 M2_monDom  RARPGAIQRLMEVLQNYYTLVDRVPALLHHLTAI----DYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPEEATM---GEAEEEDATPAANSLPEDESQRQALVDSVFQVSVLPGNVGYLRFDEFADSSVLGTLAPYVIRQVWEPLQDTNHLIMDL
 M2_sacHar  RARPGAIQRLMEILQKYYTLVDRVPALLHHLTAI----DYSSVLTEEDLAAKLNAMLQAVSEDPRLLVRVLRPEEATV--EAEPGEESATPASVSLPESDAERQALIDSVFQVSVLPGNVGYLRFDEFADNSVLGTLAPYVLRQVWEPLQDTDHLIMDL
 M2_macEug  RARPAAIQRLMEVLQNYYTLVDRVPALLHHLTAI----DYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPEEATA--GAESREEAATAAPVPLPDGESQRQALVNSVFQVSVLPGNVGYLRFDEFADSSVLGALAPYVLQQVWEPLQDTDHLIMDL
 M2_ornAna  GAVPGAVAHLADLLRDYYALVDRVPALLRHLAAL----DLSSVLSEEDLTSRLNAGLQAASEDPRLLVRRLEPEEAERGPPRKEEEQKEEEEEDQPSPGASILPGDGSSLFRVSVLPGNVGYLCFDEFPEASALERLGPLLGRRVWEPLEATDHLMVDL
 M2_galGal  RAVPGTLSRLTDILKDYYSLVERVPVLLRHLTTS----DFSSVQSAEDLATKLNTEMQTLSEDPRLLVRTMMPGEA-----AAPPAEMPIAMAANLPDNEQLLHALVDTVFKVSVLPGNVGYMRFDEFADASVLVKLGPYIVKKVWEPLQNTENLIMDL
 M2_taeGut  RAVPGTISHLKNILKDYYSLVERVPALLRRLTTS----DFSSVQSSEDLATKLNTELQALSDDPRLMVRVMMPGEA-----ADSPAEKPVGMAADLPDNEQLLHALVDTVFKVSVLPGNVGYMRFDEFADASVLVKLGPYLVHKVWEPLQNTENLIMDL
 M2_anoCar  KAIPNSMSYLVDIIKNNYSMLEQVPVLLQHLSTF----DYSSVLSVKDLASKLNAELQTISEDPRLFLRVPASDEA-----VTSQTDEKVAMASDLPNNEQLMKALVMTVFKVSVLPGNVGYMRFDEFGDATVLVKLGPYLLQHVWEPLQATDYLIIDL
 M2_xenTro  SSITHILLQLSEILVNNYAFSERIPTLLQHLPNL----DYSSVISEEDITAKLNYELQSLTEDPRLVLKSKTDSLV---------MPEDSTQVENLPDDEATLQALVNTVFKVSILPGNIGYLRFDEFADVSVLAKLGPYIVNTVWDPITVTENLIIDL
 M2_xenLae  SSVTHVLHQLCDILANNYAFSERIPTLLQHLPNL----DYSTVISEEDIAAKLNYELQSLTEDPRLVLKSKTDTLV---------MPGDSIQAENIPEDEAMLQALVNTVFKVSILPGNIGYLRFDQFADVSVIAKLAPFIVNTVWEPITITENLIIDL
 M2_petMar  GVARKAVEAAGELLLSSYTFVERASAIADHLSWS----EYGSVVSVEDLTSKLTQDLQSVAEDPRLVVSNREPEWV--------GAADPPGPPAPLPDDEQMLEAIVDSAFKVEVLEGNIGYLRFDEFGDASAVMKLRKQLVSKVWERIHPTDDVIIDL</font>
 M2x_takRu  SRIPKVLQIVLDIIGRFYAFADRVQALLQQLESA----DLFSVVSEEDLAARLNHDLQTASEDPRLIIRHKRDNI------------PRAEEEPELHAANDHDGELVEG-FTVQVLPHNTGYLRLDRFVRCSEGDKLEEIVAEKVWGPLKDTQNLIIDL
 M2x_danRe  KTIPKAVRRVSDIIKRYYSFKDKIPALLNQLAKA----DYFTVVSEEDLAGKLNHEMQSVFEDPRLLIKATQVLT------------DDASSE-DRSSSDDLTDPL----FKLEMISGNNGYLRFDRFPTPEVLLRLEDHIKKKIWQPVQETENLVIDL
 <font color="green">M3_homSap  QSLGALVEGTGHLLEAHYARPEVVGQTSALLRAKLAQGAYRTAVDLESLASQLTADLQEVSGDHRLLVFHSPGELV---------VEEAP-PPPPAVPSPEELTYLIEALFKTEVLPGQLGYLRFDAMAELETVKAVGPQLVRLVWQQLVDTAALVIDL
 M3_bosTau  RSLGELVEGTGRLLEAHYARPEVVGQMGALLRAKLAQGAYRTAVDLESLASQLTADLQEMSGDHRLLVFHSPGEMV---------AEEAP-PPPPVVPSPEELSYLIEALFKTEVLPGQLGYLRFDAMAELETVKAVGPQLVQLVWQKLVDTAALVVDL
 M3_monDom  QRLGALVEGTGHLLEAHYALPEVVGQASALLKAKLEHGTYRTAVDFESLASQLTSDLQEVSGDHRLHVFHSPGEPV---------SEELT-PPQKGVPSPEELTYLIEALFKTEVLPGQLGYLRFDMMAEAETVRAIAPQLVELVWEKLVHTEALVVDL
 M3_macEug  QRLGALVEDAGHLLEAHYALPEVVGQASALLRARLVHGTYRTAVDFESLASQLTSDLQEVSGDHRVHVFHSPGELI---------PEELS-PPQNVVPSPEELTYLIEALFKTEVLPGQLGYLRFDMMAEAETVRAIGPQLIELVWEKLVNTEALVVDL
 M3_sacHar  QRLGALVEGTGHLVEAHYALPEVVGQASAFLRATLAHGTYRTAVDFESLASQLTSDLQEVSGDHRLHVFHSPGEPV---------PEESS-PPHKGVPSPEELTYLIEALFKTDVLP-QLGYLRFDMMAEVETVRAIGPQLVELVWEKLVNTEALVVDL
 M3_taeGut  KNMGVLLEGTGQLLEDHYAIPEVAAKASAMLSTKRAQGGYRSAIDSETLASQLTSDLQEASGDHRLHVFHSHVEPT---------PEEQL-PNV--IPSPEELSYIIEALFKIEVLPGNLGYLRFDMMAEAETVKAIGPQLLQMVWNKLVDTDAMIIDM
 M3_galGal  RKMGILLESTGQLLEAHYAIPEVAEKASVMLSTKRVQGGYRSAVDFETLASQLTSDLQEASGDHRLHVFHSHVEPT---------PEEQL-PNM--IPSPEELSYIIEALFKIEVLPGNLGYLRFDMMAEAETVKAIGPQLVQMVWNKLVDTDAMIIDM
 M3_anoCar  KGMGSLIERVGQLLEAHYAIPEMARRVSSMLNSKLAQGGYRTAVDFETLASQLTNDLQETSGDHQLHVFHSHVEPS---------LEEQS-PFK--TLTPEELNFIIEALFKVDVLPGNVGYLRFDMMAEFESVKTIEPQILHMVWEKLVETSAMIVDM
 M3_xenTro  PSVFALVEGTGHLLEVHYAIPEVAYKVSSVLQNKWSEGGYRSVVDLESLASQLTSEMQENSGDHRLHVFYSDTEPE---------ILEDQ-PPK--IPSAEELNYIIDALFKIEVLQGNVGYLRFDMMADTEIIKAIGPQLVSLVWNKLVETNSLIIDM
 M3_xenLae  PSIFPLVKGTGHLLEVHYAIPEVAYKVSSVLQNKWSEGGYRSVVDLESLASLLTSEMQENSGDHRLHVFYSDTEPE---------ILEDQ-PPK--IPSPEELNYIIDALFKIEVLPGNVGYLRFDMMADTEIIKAIGPQLVSLVWNKLVETNSLIIDM
 M3_petMar  AKMASLLELAGALVEGYYAMLSDGENATAEILLKYREGWYRSVVDYEALASQLTSDLHEIWGDHRLHAF-YSDLQI---------ERMDE-DKTPSVPSPEELSVLIDTVFKVDILANNVGYLRFDMMTDAEVLKHVGPQLVEKVWNKISSTRSLVIDV</font>
 M3x_takRu  QGLRSLIGRTGELLEKHYAIQEVAQKVGEVLLSKWAEGLYRSVVDLESLASQLTADLQEASGDHRLHVFRCDVELE---------SLHGV-PK---IAAVEEAGFVIDALFKSELLPRNVGYLRFDTMADIEAAKGAAPRLVKSVWNKLVDTDSLIIDM
 M3x_danRr  KNIQGLVQEAGDLLEKHYSVPEVAAKVSRLLQSKLTEGLYRSVVDYESLASQLTSDLQETSGDQRLHIFYCETEPE---------TLHDT-PK---IPSPEEAGFIVEALFKVDVMSGNIGYLRFDMMEDIKVLQAINPEFLKVVWNKLVNTDMLIIDV
 <font color="brown">M4_petMar  ADAPSILRTVGKLVADGYSRAEAALGVPSKLAALLEAGEYGALRSEEELAFKLTVHLQLITGDRHLKAVCVPEHAT---------DRMPG-IVPMQMPPTESFEDLIKFSFITDVLEGNIGYLRFDLFSDLEALEHVAHLLVEHVWKKICDTEILIIDL
 M4_homSap  AKVPTVLQTAGKLVADNYASAELGAKMATKLSGL--QSRYSRVTSEVALAEILGADLQMLSGDPHLKAAHIPENAK---------DRIPGIVPMQ-IPSPEVFEELIKFSFHTNVLEDNIGYLRFDMFGDGELLTQVSRLLVEHIWKKIMHTDAMRIID
 M4_bosTau  AKVPTVLQTAGKLVADNYASPELGVKMAAELSGL--QSRYARVTSEAALAELLQADLQVLSGDPHLKTAHIPEDAK---------DRIPGIVPMQ-IPSPEVFEDLIKFSFHTNVLEGNVGYLRFDMFGDCELLTQVSELLVEHVWKKIVHTDALIVDM
 M4_monDom  AKVPTILQTAGKLVADNYASLEVGSRVASKLAKL--QTQYRQVTSEGELADMLGADLQTLSGDRHLKTAHIPEDAK---------DRIPGIVPMQ-LPSPEAFEDLIKFSFHTNVFEGNIGYLRFDMFGDCELLTQVSDLLVEHVWKKVVHTDGMIIDM
 M4_macEug  SKVPTILQTAGKLVADNYASPEVGSRVAAKLARL--QTQYRQVTSEGELADMLGADLQTLSGDSHLKTAHIPEDSK---------DRIPGIVPMQ-LPSPEAFEDLIKFSFHTNVFEGNIGYLRFDMFGDCELLTQVSDLLVEHVWKKVVHTDGMIIDM
 M4_sacHar  AKVPTILQTAGKLVADNYASPEVGSRVAAKLASL--QIQYGKVTSEGELADMLGADLQTLSGDRHLKTAHIPEDAK---------DRIPGIVPMQ-LPSPEAFEDLIKFSFHTNVFEGNIGYLRFDMFGDCELLIQVSDLLVEHVWKKVMHTDGMIIDM
 M4_ornAna  SKVPTVLRTAAKLVADNYAFRETGAGVAAQMGGL--QARCGRVTSEGALAEVLGAHLRALSGDPHLQMVYIPEDAK---------DRIPGVVPMQ-IPSAETFEDLIKFSFHTSVMEGNIGYLRFDMFGDCELLTQVSELMVEHVWKKIVHTDGLIIDM
 M4_xenLae  TKIPTVIQTAAKLVADNYAFADTGANVASKFIALVDKIDYKMIKSEVELAEKINDDLQSLSKDFHLKAVYIPENSK---------DRIPGVVPMQ-IPSPELFEELIKFSFHTDVFEKNIGYIRFDMFADSDLLNQVSDLLVEHVWKKVVDQDALIIDM
 M4_xenTro  TKIPSVIQTAGKLVADNYAFADTGADVASKLIALVDKINYKMIKSEVELAEKLNYDLQSLSKDVHLKAVYIPENSK---------DRIPGVVPMQ-IPSPEMFEDLIKFSFHTDVFEKNLGYIRFDMFADSDLLNQVSDLLVEHVWKKVVNQDALIIDM
 M4_taeGut  AQVPQILQTVGKLVADNYAFVNTGTVIASNLTKNIHKDNYKRINTEEDLAGKVTAILQALSDDKHLKLLYIPEHAK---------DSIPGIMPKQ-IPPPEVFEDLIKFSFHTNVFENNIGYLRFDMFGDSELLTQLSDLMIEHVWKKIFHTDALIIDL
 M4_galGal  TQVPQIVQTVGKLVAENYAFVDIGTDIASNLTKSVNKENYKRINSEKELARKLTAILQALSDDEHLKILYIPEHAK---------DSIPGILPKQ-IPSPEVFEDLIKFSFHTNVFENNIGYLRFDMFGDCELLTQVSDLLVEHVWKKIVHTDALIIDM
 M4_anoCar  TKLPSVLNTIGKLVADNYAFADIGATVAAKFADYAKKGTYRKINSEIELSGKLAADLKALSGDRHLMISHIPERSK---------GRILGLVPMQQIPPPEILEDLIKFSLHTNVFENNIGYLRFDMFGDCELMSQVSELLVQHVWNKIVNTDALIIDM
 M4_tetNig  AQIPAIIEGTAALVANNYAFEATGADVAKELRELQANGQYSSVVSKESLEAALSADLQRLSGDKSLKTT----------------PNTPVLPPMD--YTPEMYIELIKVSFHTDVFENNIGYLRFDMFGDFEEVKAIAQIIVEHVWNKVVNTDALILDL
 M4_takRub  AQIPAIIEGAATLIAKNYAFEATGADVATKLRELLAKGQYNSVVSSESLEVALSADLQRLSGDKSLKAT----------------QNAPVLPPMD--YSPEMYIELIKVSFHTDVFENNIGYLRFDMFGDFEEVKAIAQIIVEHVWNKVVNTDALILDL
 M4_gasAcu  NRVPAIIEGSATLIADNYAFEDIGAAVAEKLKGLLANGEYSKVVSKDSLEMKLSADLRTLSGDKSLKTT----------------SNVPALPPMN--YSPEMYIELIKVSFHTDVFEDNIGYLRFDMFGDFEEVKAIAQIIVEHVWNKVVNTDAMIVDL
 M4_oryLat  LQVPAIIEESATLVANNYAFESTAADVAEKLKGHLANGDYNMVVSKESLEAKLSADLQSLSGDKSLTVS----------------SNTGAPPPME--YTPEMYIELIKISFHTDVFENNIGYLRFDMFGDFEEVKAIAQVIVEHVWNKVLHTDAMIIDL
 M4_danRer  AEIPALAQAAATLIADNYAFPSIGEHVAEKLEAVVAGGEYNLISTKEDLEERLSEDLLKLSEDKCLKTT----------------SNIPALPPMN--PTPEMFIALIKSSFQTDVFENNIGYLRFDMFGDFEHVATIAQIIVEHVWNKVVDTDALIIDL</font>
 
 <font color="blue">M1_homSap  RHCTGGQVS-GIPYIISYLHPGN-TILHVDTIYNRPSNTTTEIWTLPQVLGERYGADKDVVVLTSSQTRGVAEDIAHILKQMRRAIVVGERTGGGALDLRKLRIGES------DFFFTVPVSRSLGPLGGGSQTWEGSGVLPCVGTPAEQALEKALAIL
 M1_bosTau  RHCTGGHVS-GIPYVISYLHPGS-TVSHVDTVYDRPSNTTTEIWTLPEALGEKYSADKDVVVLTSSRTGGVAEDIAYILKQMRRAIVVGERTVGGALNLQKLRVGQS------DFFLTVPVSRSLGPLGEGSQTWEGSGVLPCVGTPAEQALEKALAVL
 M1_monDom  QHSSGGEIS-GIPFVISYLHQGD-ILLHVDTVYDRPSNTTTEIWTLPQVLGERYGGEKDMVVLTSHRTVGVAEDIAYILKKLRRAIVVGEQTLGGALDLRKLRIGQS------DFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALGKALAIL
 M1_macEug  QHSTEGEVS-GIPFVISYLHEGD-ILLHVDTVYDRPSNTTTEIWTLPQVLGERYSGEKDLVILTSHRTVGVAEDIAYILKKMRRAIVVGEQTLGGALDLRKLRIGQS------DFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKALAIL
 M1_sacHar  QHSRGGEVS-GIPFVISYLHQGD-ILLHVDTIYDRPSNTTTEIWTLPQVLGERYSGEKDIVVLTSHHTVGVAEDIAYILKKMRRAIVVGEQTQGGSLDLRKLRIGQS------DFFITVPVARSLSPLGGGSQTWEGSGVVPCVGIPAEQALEKALAIL
 M1_galGal  RYSTGGQIS-GIPFIISYLHEAD-KMLHVETVYNRPSNTTTEIWTLPKVLGERYSKDKDVIVLISHHTTGVAEDVAYILKHMNRAITLGEKTAGGSLDIQKLRIGPS------NFYMMVPVSRSVSPLSGGGQSWEVSGVMPCVASEAEQALKKSLDIL
 M1_taeGut  RHSTGGQIS-GLPFIISYLHEQD-KILHVETVYNRPSNTTTEIWTLPKVLGERYSKDKDVIVLISHHTTGVAEDVAYILKHMNRAITVGEKTAGGSLDIQKLRIGPS------NFYMMVPVSRSVSPLSGGGQSWEVSGVMPCVATEAEQALQKSLDIL
 M1_anoCar  RYTTGGDVS-GIPFIISYLYNGD-KVLHVDTVYNRPSNTTVEILTLPKVLGVRYSKDKDVILLISKYTTGVAENVAYILKHMHRTIIVGEKSAGGSLDTQKMQIGNS------QFYMTVPLSCSVSPLSGSGQSWEISGVTPCVVISAEQALDKALAIL
 M1_ornAna  RHSTGGHVS-GIPFFISYFYPEG-PALHVDTVYDRPSNATRQLWTLPRVLGARYAADKDVVVLTSRLTAGVAEDVAYILQQMRRAIVVGERTAGGPLVFRKLRVGLS------DFFITVPVACSLGPLGGGGRSWEGSGVLPCVAVPADRALDEALDIL
 M1_xenLae  RYSTQGEVS-GIPFVVSYL--CD-SEIHIDSIYNRPSNTTTDLWTLPELMGERYGKVKDVVVLTSKYTKGVAEDASYILKHMNRAIVVGEKTAGGSLDTQKIKIGQS------DFYITVPVSRSLSPLTG--QSWEVSGVSPCVVVNAKDALDKAQAIL
 M1_xenTro  RYSTQGKVS-GIPFVVSYL--TD-PQIHIDSIYNRPSNTTTDLWTLSELMGERYGKDKDVVVLTSKYTEGIAEGAAYILKHMSRAIVVGEKTAGGSLDIQKIKIGQS------EFYITVPVSRSISPLTG--QSWEVAGVFPCVVVNANNALNKAQGIL
 M1_tetNig  RYTSSGDIS-GIPYIVSYFTQAE-PVVHIDSVYDRPSNTTTKLLSLPNLLGQRYGVSKPLIVLTSKNTKGIAEDVAYCLKNLKRATIVGEKTAGGSLKLDTFKVGDT------DFYITVPTAKSINPITG--SSWEIRGVTPHVEVNAEDALATAIKIV
 M1_takRub  RYTSSGEIS-GIPYIVSYFTQAE-PVVHIDSVYDRPSNTTTKLFSLSNLLGERYGITKPLIILTSKNTKGIAEDVAYCLKNLKRATIVGERTAGGSVKLDNFKVGST------DFYITVPTAKSINPVTG--SSWEITGVKPDVEVNAEDALATAIKIV
 M1_gasAcu  RYTSSGDIS-GIPYIVSYFTEAG-TPIHIDSIYDRPSNTTTKLFSMSTLLGERYSTSKPLIILTSKNTKGIAEDVAYCLQNLKRATIVGEKTAGGSVKVDKIQVRDT------GFYVTVPTAKSVNPITG--STWEVTGVTPNVEVNAEDALATAIKIV
 M1_oryLat  RYTSSGDIT-GIPYIISYLTDAK-SEIHIDTIYDRPLNTTTKLLSMQSTLGQTYGGTKPLLVLTSKNTKDIAEDVAYCLKNLKRATIVGEKTAGGSAKIKKFRVGDT------DFYVTLPTAKSINPITG--SSWEVTGVKPNVEVNAEEALATALKII
 M1_danRer  RSTVTGELS-GIPYIVSYFTDPE-PLIHIDSVYDRTADLTIELWSMPTLLGKRYGTSKPLIILTSKDTLGIAEDVAYCLKNLKRATIVGENTAGGTVKMSKMKVGDT------DFYVTVPVAKSINPITG--KSWEINGVAPDVDVAAEDALDAAIAII
 M1_petMar  RYNSHGDIT-GLPYLVSCFCEPR-PVVHLDTVYYRPTNESKEIWSLPDLQGARFAKHKDVFVLVSANTEGVAENVAYVLKHLHRATVIGEQTAGGSLEVERFRLGDS------RFFVTVPTARS-EPAD---RSW---GVFPCVSAPSERALDKALEIL</font>
 M1x_takRu  RFSTAGGWS-GIPSIVSYFTEPH-SLVHIDTVYDRPSNTTTELWTMSSVRGKTFGGKKDMIVLIGRRTAGAAEAVAYTLKHLNRAIVVGERSAGGSLKVRKFRIAES------DFYITMPVARSVSPITG--KSWEVSGISPTVNVAAREALAKAQTFL
 M1x_danRe  RFSTAGELS-GLPYIVSYFSDSD-PLLHIDTIYERPTNITRELWTLPTLLGERFGKRKDLIVLISKRTIGAAEGVAYILKHLKRAVIIGERSAGGSVRVDKLKIGDS------GFYITVPVARSVNPVTG--QSWEVSGVAPSVTVNPKESIAKAKSLI
 <font color="red">M2_homSap  RHNPGGPSS-AVPLLLSYFQGPEAGPVHLFTTYDRRTNITQEHFSHMELPGPRYSTQRGVYLLTSHRTATAAEEFAFLMQSLGWATLVGEITAGNLLHTRTVPLLDTPEG---SLALTVPVLTFIDNHG---EAWLGGGVVPDAIVLAEEALDKAQEVL
 M2_bosTau  RQNPGGPSS-AVPLLLSYFQSPDASPVRLFSTYDRRTNITREHFSQTELLGRPYGTQRGVYLLTSHRTATAAEELAFLMQSLGWATLVGEITAGSLLHTHTVSLLETPEG---GLALTVPVLTFIDNHG---ECWLGGGVVPDAIVLAEEALDRAQEVL
 M2_monDom  RYNPGGPSS-AVPLLLSYFQDPAAGPIRLFTTYDRQTNQTQEHLSRAELLGKPYGAQRGVYLLTSHHTATAAEEFAFLMQSLGRATLVGEITAGSLMHTRTFPLLQPPNG---NLVLTVPILTFIDNNG---ECWLGGGVVPDAIVLAEEALDKAKEVL
 M2_sacHar  RYNPGGPSS-AVPLLLSYFQDPSAGPVRLFATYDRQTNQTQEYRSRAELLGKPYGAERGVYLLTSYHTATAAEEFAFLMQSLGRATLVGEITAGSLMHTRTFPLLQPPNG---SLVLTVPTLTFIDNHG---ECWLGGGVVPDAIVLAEEALDKAKEVL
 M2_macEug  RYNPGGPSS-AVPLLLSYFQDPAAGPVRLFATYDRQTNQTREYRSQAELLGKPYGAQRGVYLLTSHHTATAAEEFAFLMQSLGRATLVGEITAGNLMHTRTFSLLQPPDG---SLVLTVPILTFIDNHG---ECWLGGGVVPDAIVLAEEALDKAKEVI
 M2_galGal  RYNPGGPSSSAVPMLISYFQDPTAGPVHLFTTYDRRTNHTQEHNSQAELLAQPYGAQRGIYVLTSRHTATAAEEFAYLMQSLGRATLIGEITAGSLSHTCTFPLVQPEQGITRGLTITVPVITFIDNHG---ESWMGGGVVPDAIVLAEDALEKAEEVL
 M2_taeGut  RYNLGGPSSSAVPVLLSYFQDPAAGPVHLFTTYDRRTNHTQEHNSQAELLGQSYGAKRGVYLLTSHHTATAAEEFAYLMQSLGRATLIGEITAGSLSHTRTFPLLQPGPGITRGLTITVPVITFIDNHG---ESWMGGGVVPDAIVLAEDALEKAEEVL
 M2_anoCar  RYNIGGPSSSAVPVLLSYFQDPSAGPVHFFTTYNRLTNQTQAYSSSAEMVGKPYGARRGVYLLTSHNTATAAEEFAYLMQTLGRATLVGEITAGSLSHTHTFCILELGGGC--GLLINVPVITLIDNHG---EYWLGGGVVPDSIVLADEALEKAREVL
 M2_ornAna  RNNPGGPSS-AVPLLLSYFQDPAAGPIRLFTTYNRPADVTREYASRAGALEKPYGARRGVYLLTSHRTATAAEEFAYLMQALGRATLVGEITAGRLLHSRTFPLLRPPWE---GLVLTVPFLTLFDPHG---EGWLGGGVVPDAIVLAEEALEKAGEVL
 M2_xenTro  RYNIGG-SSTSIPLLLSYFQEPE-NRIHLFTIYNRQQNSTNEVYSLPKVLGKPYGSKKGVYVLTSHETATAAEEFAYLMQSLSRATIIGEITSGNLMHSKAFPLDGTR------LSVTVPIMNFIDNNG---DYWLGGGVVPDAIVLADEALDKAKEII
 M2_xenLae  RYNVGG-SSTAVPLLLSYFLDPE-TKIHLFTLHNRQQNSTDEVYSHPKVLGKPYGSKKGVYVLTSHQTATAAEEFAYLMQSLSRATIIGEITSGNLMHSKVFPFDGTQ------LSVTVPIINFIDSNG---DYWLGGGVVPDAIVLADEALDKAKEII
 M2_petMar  RYNLGG-SSTAIPIVLSYFQDVA--PVHFYTVYDRLRNVTAEFHTVSNLTSQLYGSKKGVYLLTSQHTATAAEEFTYLMQSLNRATIVGEITSGRLAHSLAFRLSDT------GLYMTVPIVNFIDNND---EYWLGGGVVPDAIVLAENALDAAKEII</font>
 M2x_takRu  RHNTGG-SSTSVALLLSYLRDPL-PKRHFFTIYDSVQNTTTEYGSRPHIPGPSYGSERGVYVLTSHYTAGAAEEFAYLIQSLHFGTVVGEITSGTLMHSKTFQVEGT------DIFITVPFINFLDNNG---EYWLGGGVVPDAIVLAEEALEHVNRTA
 M2x_danRe  RFNTGG-STEALPILLSYMFDTS-SSTYLFSIYDSIKNTTFDFHTLNNISGPSYGSTKGVYVLTSYYTAEAGEEFAYLMQSLHRGTVIGEITSGMLLHSKTFQIEQT------SLAITVPIINFIDVNG---ECWLGGGVVPDAIVLAEEALERAHEII
 <font color="green">M3_homSap  RYNPGSYST-AIPLLCSYFFEAE-PRQHLYSVFDRATSKVTEVWTLPQVAGQRYGSHKDLYILMSHTSGSAAEAFAHTMQDLQRATVIGEPTAGGALSVGIYQVGSS------PLYASMPTQMAMSATTG--KAWDLAGVEPDITVPMSEALSIAQDIV
 M3_bosTau  RYNPGSYST-AVPLLCSYFFEAE-PRRHLYSVFDRATSRVTEVWTLPHVTGQRYGSHKDLYVLVSHTSGSAAEAFAHTMQDLQRATIIGEPTAGGALSVGIYQVGSS------ALYASMPTQMAMSASTG--EAWDLAGVEPDITVPMSVALSTARDIV
 M3_monDom  RYNPGGYST-AVPLLCSYFFEAE-PRRHLYTIFDRAASQLTEVWTLPQVAGERYGSQKDLYILISHTSGSAAEAFVHTMKDQHRATVIGEPTGGGALSVGIYQVENS------PLYASMPTQVAISPVTG--KAWDMAGVEPDVSVLSSEALMTTQGIV
 M3_macEug  RYNPGSYST-SVPLLCSYFFEAE-PRKHLYTIFDRAASQATEVWTLPQVAGERYGSQKDLYILISHTSGSAAEAFAHAMKDLRRATVIGEPTAGGALSVGIYQVSNS------PLYASMPTQVAISPVTG--KAWDIAGVEPDVSVPAREALITTQGIV
 M3_sacHar  RYNPGSYST-TVPLLCSYFFEAE-PRKHLYTVFDRATSQFTEVWTLPQVTGERYGSQKDLYILISHTSGSAAEAFAHIMKDLQRATVIGEPTAGGALSVGIYQVGDS------PLYVSMPTQVALSPVTG--KAWDMAGVEPDVSVLANEALITAQGIV
 M3_taeGut  RYNTGGYST-AIPILCSYFFDPE-PRKHLYTVFDRSTSRSTEVWTLPQLAGKRYGSLKDIYILTSHMSGSAAEAFTRSMKDLHRATVVGEPTVGGSLSVGIYRVGNS------SLYASIPSQVVLSPVTG--KVWSVSGVEPHITIQASEAMAAAQHIA
 M3_galGal  RYNTGGYST-AVPILCSYFFEPE-PRQHLYTVFDRSTSRSTEVWTLPKVTGKRYGSLKDIYILTSHMSGSAAEAFTRSMKDLHRATVIGEPTVGGSLSVGIYRVGNS------SLYRSIPSQVVLSPVTG--KVWSVSGAEPHITIQASEALAAAKHIA
 M3_anoCar  RYNTGSYST-AVPMFCSYFFDAE-PQQHLYTIIDRSTSQSTEVWTSSQVSGKRYGSTKDLYILISHASGSAAEAFTRSLKDLHRATVIGEPTVGGSLSASIYNIGST------PLYASIPSQIVLSPVSG--KVWSLSGIQPHVTTQSNEALASAQNII
 M3_xenTro  RYNTGGYST-AIPIFCSYFFDPE-PLQHLYTVYDRSTSSGTDIWTLPEVVGERYGSTKDIYILTSHMTGSAAEVFTRSMKELNRATIIGEPTSGVSLSVGMYKVGES------NLYVSIPNQVVISSVTG--KVWSVSGVEPHVIAQASEAMNVAHHII
 M3_xenLae  RYNTGGYST-AIPIFCSYFFDPE-PLQHLYTVYDRSTSTGKDIWTLPEVFGERYGSTKDIYILTSHMTGSAAEVFTRSLKDLNRATLIGEPTSGVSLSVGMYKVGDS------NLYVTIPNQVVISSVTG--KVWSVSGVEPHVIIQANEAMNIAHRII
 M3_petMar  RYNMGGYST-SIPILCSYFFDAS-PPRHLYTVFDRPSRSSTQVFTVPRVLGQRYGASKDVYILTSHMTGSAGEILTRVMSDLKRATVIGEPTAGGSLSTGTYRIGDS------RLYVFIPNQAGVSPSGG--RTWSVAGVEPHVQTKASEALQSALRMV</font>
 M3x_takRu  RYNAGGSST-AVPLWCSYFVDGE-PLQHLYTVYDRTTKTRVEVMTLPEVSGQRYDPGKDVYILTSHMTGSAAEAFVRAMRDLNRVTIVGEPTAGGSLSSATYQIGES------VLYASIPNQVVTSAATG--KLWSISGVEPDVFAQARDALPVAQRII
 M3x_danRr  RYNTGGYST-AIPLLCTYFFDAQ-PLTHIYTLFDRSTATVTKVTTLPDVLGQKYSSQKDVYILTSHITGSAAEAFTRTMKDLKRATVIGEPTIGGALSSGTYQIGNS------ILYASIPNQAVLNAVTG--KPWSISGVEPHIVAQASDALIVAQKII
 <font color="brown">M4_petMar  RYNMGGYST-SIPILCSYFFDAS-PPRHLYTVFDRPSRSSTQVFTVPRVLGQRYGASKDVYILTSHMTGSAGEILTRVMSDLKRATVIGEPTAGGSLSTGTYRIGDS------RLYVFIPNQAGVSPSGG--RTWSVAGVEPHVQTKASEALQSALRMV
 M4_homSap  MFNIGGPTS-SIPILCSYFFDEG-PPVLLDKIYSRPDDSVSELWTHAQVVGERYGSKKSMVILTSSVTAGTAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDT------NLYLTIPTARSVGASDG--SSWEGVGVTPHVVVPAEEALARAKEML
 M4_bosTau  RFNIGGPTS-SISALCSYFFDEG-PPILLDKIYNRPNNSVSELWTLSQLEGERYGSKKSMVILTSTLTAGAAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDT------DLYLTIPTARSVGAADG--SSWEGVGVVPDVAVPAEAALTRAQEML
 M4_monDom  RFNIGGPTS-SISALCSYFFDEG-QEVLLDQIYNRPNDSISEIWTQSQVAGERYGSKKSVIILTSSMTAGAAEEFVYVMQRLGRALVIGEVTSGGCQPPQTYHVDDT------DLYITIPTARSVGSGDK--PSWEGVGVAPHVEVPADQALSKAKEMF
 M4_macEug  RFNIGGPTS-SISALCSYFFDEG-QKVLLDRIYNRPNDSIVEIWTQPHVTGERYGSKKSVIILTSSTTAGAAEEFVYIMQGLGRALVIGEVTSGGCQPPQTYHVDDT------DLYITIPTAQSVGSGDR--PSWEGIGVTPHVEVPADQALSKAKEMF
 M4_sacHar  RFNIGGPTS-SISAMCSYFFDEG-QGVLLDRIYNRPNDSISEIWTQPQVIGERYGSKKTVVILTSSMTAGAAEEFVYIMQRLGRALVIGEVTSGGCQPPQTYHVDDT------DLYITIPTTRSVVSGDK--SSWEGVGVVPHMEVPADQALSKAKEMF
 M4_ornAna  R-NIGGPTS-SISALCSYFFDED-HPVLLDKIYNRPNDSISEIWTHSHIAGERYGSRKSVVILTSNMTAGAAEEFVSIMKRLGRALVVGEVTGGGCHPPQTYHVDDT------HLYITIPTSRSVGSEDG--SSWEGVGVTPHLVVPADVALSRAKDLF
 M4_xenLae  RFNIGGPTS-SIPIFCSYFFDEG-TPVLLDKIYSRTSNAMTDIWTLPDLVGKTFGSKKPLIILTSSLTEGAAEEFVYIMKRLGRAYVVGEVTSGGCHPPQTYHVDDT------HLYLTIPTSRSASAEPG--ESWEGKGVLPDLEISSETALLKAKEIL
 M4_xenTro  RFNIGGPTS-SIPTFCSYFFDEG-TPVLLDKIYSRTTNAITDVWTLPHLVGNAFGSKKPVIILTSSLTEGAAEEFVYIMKRLGRAYVIGEVTSGGCHPPQTYHVDDT------HLYLTIPTSRSASAKPG--ESWEGKGVLPDLEITSETALMKAKEIL
 M4_taeGut  RYNIGGSTT-PIAILCSYFFDEG-HPVLLDRVYDRPSDSVKEIWTQPQLKGERYGSQKGLVILTSAVTAGAAEEFVYIMKRLSRALIIGEQTSGGCHSPQTYQVDET------NFYVVIPTSRSVTSADS--TSWEGKGVSPHIETPAETALIKAKEML
 M4_galGal  RYNIGGYTN-SIPILCSYFFDEG-HQVLLDKVYDRPSDSVKEIWTQPQLRGERYGSQKGLIILTSAVTAGAAEEFVFIMKRLGRALIIGEQTSGGSHSPQTYQVDDT------NFYIIIPTARSVISAES--ASWEGKGVPPHMETPAVTALIKAKEVL
 M4_anoCar  RYNVGGPAC-SVPLLCSYFFDEG-HPILLDKVYNRPNDTTSNIWTVSKLAGKRYGLNKGLIILTSSVTSGAAEEFAHIMKRLGRAFIIGQKTSGGCHPPQTFHVDGT------NLYITTPVSRSVFSVN---DSWEGVGVSPHLDVSTDVALIKAKEML
 M4_tetNig  RNNVGGPTT-AIAGFCSYFFDAD-KQNRVGQAVRQASGTTTELLTLSELTGVRYGSKKSLIILTSGATAGAAEEFVYIMKKLGRAMIVGETTAGASHPPQTFRVGET------DVFLLIPTVHSDTGA-G--PAWEGAGIAPHIPASAEAALGTARAIL
 M4_takRub  RNNVGGPTT-AIAGFCSYFFDAD-KLIVLDKLHDRPSGTTTELLTLPELTGVRYGSKKSLIILTSGATAGAAEEFVYIMKKLGRAMIVGETTAGASHPPQVFSVGEI------GIFLSIPTVHSDTAA-G--PAWEGTGITPHIPVSAEAALGTAKGIL
 M4_gasAcu  RNNIGGPTT-AIAGFCSYFFDSD-KQIVLDRLYDRPSGTTTELRTLPELTGTRYGSKKSLVMLTSRATAGAAEEFVYIMKKLGRAMIVGETTAGTSHPPKTFRVGET------DIFLSIPTVHSDTAA-G--PAWEGAGVAPHIPVPADAALETAKGIF
 M4_oryLat  RNNVGGPTT-AIAGFCSYFFDGD-KQILLDKLYDRSTGTTTDLLTLGELTGERYGSKKSLIILASRATAGAAEEFVYIMKRLGRAMIVGETTAGASHPPKVFQVGES------DIFLSIPTVHSDTSA-G--PGWEGAGVAPHIPVAAGAALETAKAIL
 M4_danRer  RNNIGGHAS-SIAGFCSYFFDAD-KQIVLDHIYDRPSNTTRDLQTLEQLTGRRYGSKKSVVILTSGVTAGAAEEFVFIMKRLGRAMIIGETTHGGCQPPETFAVGES------DIFLSIPISHS-TAQ-G--PSWEGAGIAPHIPVPAGAALDTAKGML</font>

=== Evolutionary origin of RBP3 (IRBP) ===

Lamprey sequence was recovered by a French group in 2008 from unassembled genome project contigs but not explicitly provided in the article or posted at GenBank. They reported four modules despite uncertainty in whether these all resided in the same gene. Below, a full length lamprey gene is independently recovered from the initial assembly and parsed into its modules using Superfamily HMM. It indeed has four modules classifying to the expected types and ordering, proving the gene was fully established prior to the common ancestor of mammals and lamprey. As the lamprey ancestor already had fully modern ciliary color vision (so need for retinol shuttling), the role of RBP3 (IRBP) in extant species may have already been established 500 myr ago.  

Unexpectedly, lamprey lacks the final intron: exons 3/4 are fused. While no full length chondrichthyes sequence is available, close study of elephantshark contigs establishes that exon 3 has acquired an intron with the same phase 0 and position seen in later-diverging tetrapods. While intron loss in lamprey is possible, the simpler explanation is intron gain in the stem for reasons provided below.

 Callorhinchus milii contig AAVX01012059 establishes that exon 3 has standard flanking phase 1 introns on both sides: 
 
 VIASTSSLIVDLR<font color="blue">YNIGGPTSSIPILCSYFFDDDKTVLLDTVYSRPTDTISEMKAIPQVAGNGSTESSVHSYI</font>GERYGSKKSMVIL
 +I  T+SL  D R<font color="blue">YNIGGPTSSIPILCSYFFDDDKTVLLDTVYSRPTDTISEMKAIPQVAGNGSTESSVHSYI</font> E     K  +I+
 LIIETNSL-RDHR<font color="blue">YNIGGPTSSIPILCSYFFDDDKTVLLDTVYSRPTDTISEMKAIPQVAGNGSTESSVHSYI</font>CEDLHHCKYGLII
 
Previous efforts to trace the gene back to earlier deuterostomes (or metazoa) proved futile. That remains the case in March 2009 for tunicate (with a third assembly and massive transcript set) and much-studied sea urchin, establishing that in all likelihood homologs have been lost. However it is possible but implausible that sequences have diverged to the point of unrecognizability. It is equally implausible that a complex fold matching known bacterial folds arose de novo in deuterostomes. This creates the unresolved dilemma of ghost gene retention over an immense time frame in ancestral eyeless species yet loss in almost all extant clades. 

With the advent of the second assembly of the cephalochordate Branchiostoma floridae (which has far fewer polymorphism-related assembly stutters than the first release), it is straightforward to recover a homolog in this species (after rubbishing an unmotivated fusion to the adjacent sulfotransferase in the JGI gene model). The considerably smaller gene here consists of a single module that clusters best with M3 and M4. This suggests that the module number expansion -- like so many key events in vertebrate gene evolution -- took place between cephalochordate and agnathan divergences. 

The shocking aspect of the Branchiostoma RBP3 (IRBP) gene is its 9 exons. These range in size from 30 to 66 amino acids, quite typical of the average vertebrate gene. The anomaly here is really in the immense size of the first exon of the later four-module gene which extends for 1018 residues in human. That can be placed in prospective using the UCSC [http://genome.cse.ucsc.edu/cgi-bin/hgTables Table Browser] to determine the overall size distribution of the 190,000-odd human coding exons. The average protein has 450 residues and 8-9 exons.

The second anomaly is that the placement and phasing of the Branchiostoma introns do not correspond at all via blastp alignment to those of module 4, the only vertebrate module with internal exons (3 of them). A great majority of introns are immensely conserved -- from human to cnidarian -- so the notion of massive erasure followed by de novo intronation in either the Branchiostoma or vertebrate gene can be discarded. However some explanation is required because the genes are strongly homologous (chance expectation e-30) though at best 31% identical and gappy in alignment.

The Branchiostoma gene can be assumed orthologous for lack of a better candidate, but nearby genes in the assembly (EARS2 DNAJC19 UGT2B4 CD79B) bear no obvious synteny to those flanking the human RBP3 gene (RBP3  ZNF488 GDF2 GDF10 ANXA8L1) and only broken chromosomal correspondence is seen in whole genome alignment to human (net track). 

It follows that searches for earlier diverging species that still carry a homolog should be carried out with a single-domain protein as query. The amphioxus protein is the only one currently available and may be highly diverged from its ancestral form. In any event, it does not expose any cryptic homologs in tunicate or echinoderm, much less lophotrochozoa, arthropods or cnidaria established to have ciliary opsin systems.

Under the twin assumptions of orthology and approximately ancestral intronation pattern in Branchiostoma, how do we get from a one module gene with 8 introns to a four module gene with 2 unrelated introns in lamprey? This in some ways may have been the critical step in the evolution of imaging eyes with their massive requirement for retinol recycling that vastly exceeded that of the ancestral cephalochordate. Thus the gene co-evolved its interstitial location and shuttling function with the newly developed supporting role of the retina pigmented epithelium, which together were essential for effective vertebrate imaging vision.

One hypothetical scenario is formation first of a retroprocessed intronless gene. This may have displaced the nine-exon parental gene because of selection for more efficient translation. The protein may have been initially a tetramer of discrete subunits. The gene dosage then doubled allowing the protein to become a dimer of dimers, perhaps through a transcript that read through two close tandem copies. Next the intervening untranslated region experienced deletions fusing the previously independent modules. The gene complex then doubled again perhaps through recombinational mismatch to meet the newly evolving need for rapid retinol recycling -- at this point all four modules bound retinol. After two introns were acquired in the terminal module and lamprey diverged, a third and final intron was gained. Finally a need for allosteric regulation or interaction with other proteins allowed subfunctionalization of other domains to non-retinol shuttling roles despite the apparent loss of previously selected efficiency.

While this scenario is speculation and non-unique, elements of it are not uncommon in other genes, even in opsins. For example, a retroprocessed fish RHO1 completely displaced the parental 5 exon gene and LWS repeated spawned secondary cone opsin genes that were initially tandem. A great many human proteins have internally repeated domains, some like titin quite large numbers of them. There may not exist sufficient surviving members of the cephalochordate, hagfish and lamprey  clades to specifically illuminate what happened here. The highest priority would be to study localization and function of RBP3_braFlo within that organisms visual systems.

 >RBP3_braFlo Branchiostoma floridae Region: 9 exons; 1 domain: 83-381 
 0 MTRPSKVDIVFPIKPFTIPTAHEQVKGEGPVDINKNALCKSADEGHTHP 1
 2 VSIAMAPTAYIVFVALVPTVLSVDWLD<font color="blue">VVMGIGDVMADHYLDQDLRALNDQSLLQRWNRTLVHRFQ 0
 0 SWSQDDMSDSLRMEEGLTSELRNITGDETIK 0
 0 VWDFGVYENTTQEPVPREFYNFSTFVDNFK 2
 1 KNREKHINVTMLEGNVGYVSIRSMSHIVDIILPDPEMTEFFLSKMAALNESK 0
 0 AIILDLRYNLGGDREGVVHWASFFFNATPSVPLSDVYYRDGVNQYWTLLE 0
 0 VPGGIRFPDMPLYLLTSNRTSREAEEFAYAMQVVNRTTIIGETT 1
 2 AGEEFTGMWFPIDQTDVHLLTRTNVVRNPITQDSWSGK 1
 2 GVTPDIIVPSEKALTVALRKIQ</font>GSEDTKMAASSGNIEPPRWTVYLVFICTSIAILTYPTFM* 

=== RBP3 (IRBP) use in marsupial phylogeny ===
 
The first three homology domains and part of the fourth are all encoded by the first large exon of 1090 amino acids. Sequenced fragments of this exon (often starting internally within module M1) has been [http://www.ncbi.nlm.nih.gov/pubmed/19249373 much used in marsupial phylogeny] (along with the first intron of transthyretin). Indeed the 96 marsupial species in 51 genera having partially determined IRBP sequences at GenBank include a Dec 2008 partial sequence for the extinct species [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=158668312 Thylacinus cynocephalus], as well as for [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=47117971 Sarcophilus harrisii] (replaced here by a full length sequence derived from the genome project).

The use of IRBP in phylogenetic trees requires a high level of sequence accuracy, particularly since 'informative' characters are precisely the differences. However the PCR survey sequences exhibit various discrepancies in comparison to very high coverage genomic sequences. Some differences could arise from valid polymorphisms in individual animals used as dna source or represent an acceptable (low) level of PCR sequencing error, but other discrepancies appear to be gross errors in GenBank submissions (all from the same research group). 

The Sacrophilus entry AY532685 has 6 amino acids inconsistent with genomic data (too many for polymorphic variation) as well as a gross error affecting a 17 residue block. It seems likely in the rush to obtain enough 'informative' characters, a completely unworkable error rate has been tolerated. All marsupial phylogenetic studies based in part on analysis of IRBP thus come into question.

None of the fragementary marsupial GenBank entries provide a numbering system relative to a full length marsupial IRBP gene (eg Monodelphis) or its modules; the 4x module repeat structure of the gene is conducive to erroneous module cross-alignment. cDNA sequences typically start at position 73 of M1 (beginning of first hypervariable indel region) and continue half way through M2 (to positon 143), for example EF028750 of Myrmecobius fasciatus (numbat). Thus the region commonly sequenced is poorly coordinated with internal modular structure or existing 3D data.

The closest matches to the thylacine IRBP are shown in the difference alignment of the first 60 residues below. These species all lie with the Dasyuromorphia. The indicated E-->K may be one of several phyloSNPs breaking this group into <font color="blue">blue</font> and <font color="green">green</font> subclades. The numbat <font color="red">Myrmecobius</font> fits implausibly (its amino terminal sequence [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=124054062 EF028750] needs verification) -- its affinities seem to lie with the <font color="brown">Didelphimorphia</font> in view of the shared Q.VV.K motif. Thylacinus is not basal within Dasyuromorphia relative to Myrmecobius using IRBP. This is not a case of mis-comparison of modules. 
   *           *                                     *<font color="blue">
 STSKAPQHDSKFTNATQEELLALFQQIIKYQVLEGNVGYLRVDYIPGREMIEEVGEFLVN EU091365  0 Thylacinus cynocephalus
 .........P..A..................I............................ AY532676  3 Myoictis wallacei
 ........NP..A............................................... AY532687  3 Neophascogale lorentzii
 ........NP..A........T...................................... AY532686  4 Phascolosorex dorsalis
 .........P..V............................................... AY532670  2 Parantechinus apicalis
 ....V....P..A..................I.....................L...... AY532675  5 Myoictis melas
 .........P..A...................................D........... AY532679  3 Dasyurus hallucatus
 ...E.....P..A............K........D.............D........... AY532685  6 <font color="magenta">Sarcophilus harrisii</font>
 ...E.......RA..........L............................Q..K.... EF028748  6 Sminthopsis crassicaudata
 .......R.P.LA.........SL.......................Q....Q....... EF028749  8 Planigale ingrami</font>
 <font color="green">..A......P.LA.V.....................................K....... EF028736  6 Antechinus stuartii
 ..A......P.L..V.....................................K....... EF028743  5 Micromurexia habbema
 ..A......P.LA.V.....................................K....... EF028744  6 Murexchinus melanurus
 ..A......P.L..V....V................................K....... EF028746  6 Paramurexia rothschildi
 ..A......P.LA.V.....................................K....... EF028747  6 Phascogale calura
 ..A......P.LA.V.....................................K....... EF028745  6 Phascomurexia naso
 .SA......P.LA.V.....................................K....... AY532667  7 Murexia longicaudata</font>
 <font color="red">......K..PNLA........T.L..R....................Q.VV.K....... EF028750 12 Myrmecobius fasciatus</font>
 <font color="brown">..PET...VP..A.V........L..M....................Q.VV.K....... AY233765 13 Caluromys philander
 ..PET...VP.LA.V.......QL..M....................Q.VV.K....... AF257675 15 Caluromysiops irrupta
 ..PET...VP.LA.V......T.L..M....................Q.VV.K....... AF257688 15 Glironia venusta  
 .IPET...VP..A.V.R....T.L..M....................Q.VV.K....... AF257683 16 Didelphis albiventris
 .IPE....VP.LA.I......T.L..M....................Q.VV.K....... AF257686 15 Gracilinanus microtarsus
 .IPET...VP..A.V......T.L..M....................Q.VV.K....... AF257676 15 Marmosops noctivagus
 .IPET...VP.LA.V........L..M....................Q.VV.K....... AY233788 15 Philander opossum
 .IPET...VP.LA.I......T.L..M....................Q.VV.K....... AF257689 16 Thylamys pallidior</font>

Using Sarcophilus as probe in a different region, 721-900, we find a very peculiar outcome for numerous GenBank marsupial entries: the 17 residue region orthologous to 38-54 in M2 (VLTEEDLAAKLNAMLQA in M2_sacHar) has been replaced by a non-homologous 17 residue section copied over from 143-158 of M1 (SLVLDLQHSRGGEVSG in M2_myrFas), explaining non-alignment in the latter region (blue shows M2):

 myrFas:    61 DIWKKVMETSSL<font color="red">VLDLQHSRGGEVSG</font>IPFVISYLHQGDILLHVDTIYDRPSNTTTEIWTL 120
               DIWKKVMETSSLVLDLQHSRGGEVSGIPFVISYLHQGDILLHVDTIYDRPSNTTTEIWTL
 sacHar:   154 DIWKKVMETSSLVLDLQHSRGGEVSGIPFVISYLHQGDILLHVDTIYDRPSNTTTEIWTL 213
 
 myrFas:   121 PQVLGERYSGEKDIVVLTSHHTVGVAEDIAYILKEMRRAIVVGEQTQGGVLDLRKLRIGQ 180
               PQVLGERYSGEKDIVVLTSHHTVGVAEDIAYILK+MRRAIVVGEQTQGG LDLRKLRIGQ
 sacHar:   214 PQVLGERYSGEKDIVVLTSHHTVGVAEDIAYILKKMRRAIVVGEQTQGGSLDLRKLRIGQ 273
 
 myrFas:   181 SDFFITVPVARSLSPLGGGSQTLEGSGVVPCVGIPAEKALEKTLAILTLR<font color="blue">RARPGAIQRL 240
               SDFFITVPVARSLSPLGGGSQT EGSGVVPCVGIPAE+ALEK LAILTLR<font color="blue">RARPGAIQRL
 sacHar:   274 SDFFITVPVARSLSPLGGGSQTWEGSGVVPCVGIPAEQALEKALAILTLR<font color="blue">RARPGAIQRL 333
 
 myrFas:   241 MEILQKYYTLVDRVPALLHHLTAIDYSSSL<font color="red">VLDLQHSRGGEVSG</font>TVSEDPRLLVRVLRSE 300</font>
               MEILQKYYTLVDRVPALLHHLTAIDYSS L  +   ++   +   VSEDPRLLVRVLR E</font>
 sacHar:   334 MEILQKYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAMLQAVSEDPRLLVRVLRPE 393</font>

The source of this error cannot plausibly be explained XY difference, pseudogene, balanced polymorphism, nonhomologous recombination, frameshifts, internal inversion, or systemic experimental error (eg Dasyurus maculatus AY532680 is identical to AY243439 outside the 15 amino acid block). Genomic reads from Sarcophilus, Macropus and Monodelphis show no sign of novel substitution gene despite excellent coverage. All Didelphimorphia and Diprotodontia entries at Genbank are normal; sauropods, platypus and dozens of placentals establish this as ancestral. For some marsupials, independent sequencing finds the normal version of the gene.

These peculiar sequences were all submitted by C. Krajewski on 23-JAN-2004 and utilized in a paper entitled "Molecular systematics of the enigmatic phascolosoricine marsupials of New Guinea" published in the Aust. J. Zool. 52, 389-415 (2004). Two similarly flawed sequences submitted the same day (AY532677, AY532679) were subsequently corrected on 25-SEP-2006 by the same individual resulting in GenBank entries being fixed. However many subsequent studies of IRBP may have used the 17 flawed sequences that were never corrected. The same authors resequenced species such as Pseudantechinus roryi (EU086689) obtaining the correct sequence in this region on 07-AUG-2007 but still did not fix their earlier entry AY532673.
 
 AY532685 MEILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE <font color="blue">Sarcophilus harrisii</font>
 AY532684 ....E................................S....................P. Dasyurus geoffroii
 AY532681 ....E................................S....................P. Dasyurus albopunctatus
 AY532683 ....E................................S....................P. Dasyurus viverrinus
 AY532682 ....E........................P.......SE...................P. Dasyurus spartacus
 AY532680 ....E..............R.................SR...................P. <font color="red">Dasyurus maculatus</font>
 AY532678 ..V..................................S....................P. Dasycercus cristicauda
 AY532669 ..V..................................S....................P. Dasykaluta rosamondae
 AY532676 ..V..................S...............S....................P. Myoictis wallacei
 AY532675 ..V..................S...............S....................P. Myoictis melas
 AY532687 ..V........N.L.......................S....................P. Neophascogale lorentzii
 AY532671 ..V..................................S....................P. Parantechinus bilarni
 AY532670 ..V.................................TS.........RG.........P. Parantechinus apicalis
 AY532686 ..V..................................S........P...........p. Phascolosorex dorsalis
 AY532674 ..V.......................................................P. Pseudantechinus ningbing
 AY532672 ..V..................................S....................P. Pseudantechinus woolleyae
 AY532673 ..V........N..R......................S...................SP. <font color="magenta">Pseudantechinus roryi</font>
 454 read MEILQKYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAMLQAVSEDP           <font color="blue">Sarcophilus harrisii</font>
 EF028739 ............................V.TEEDLAAKLNAMLQA.............P. Antechinus minimus
 AY243439 ....E..............R........V.TEEDLAAKLNAMLQA.............P. <font color="red">Dasyurus maculatus</font>
 EF028750 ....K................KT.....I.TEEDLAAKLNAILQA.............P. Myrmecobius fasciatus
 EF028737 ..V.........................V.TEEDLAAKINAMLQA.............P. Antechinus flavipes
 EF028748 ..V.........................V.TEEDLAAKLNA.LQA.............P. Sminthopsis crassicaudata
 AY243438 ..V.........................V.TEEDLAAKLNA.LQA.............P. Planigale sp.
 EF028749 ..V.........................V.TEEDLAAKLNA.LQA.............P. Planigale ingrami
 AY532679 ..V.........................V.TEEDLAAKLNAMLQA............... Dasyurus hallucatus
 AF025382 ..V.........................V.TEEDLAAKLNAMLQA.............P. Phascogale tapoatafa
 EF028741 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus godmani
 AY532666 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus swainsonii
 EF028736 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus stuartii
 EF028742 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus agilis
 EF028738 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus bellus
 EF028740 ..V.........................V.TEEDLAAKLNAMLQA.............P. Antechinus leo
 EF028747 ..V.........................V.TEEDLAAKLNAMLQA.............P. Phascogale calura
 EF028744 ..V.........................V.TEEDLAAKLNAMLQA.............P. Murexchinus melanurus
 EF028743 ..V.........................V.TEEDLAAKLNAMLQA.............P. Micromurexia habbema
 EU086688 ..V.........................V.TEEDLAAKLNAMLQA.............P. Pseudantechinus macdonnellensis
 EU086689 ..V.........................V.TEEDLAAKLNAMLQA.............P. <font color="magenta">Pseudantechinus roryi</font>
 EU086686 ..V.........................V.TEEDLAAKLNAMLQA............SP. Pseudantechinus macdonnellensis
 EU086687 ..V.........................V.TEEDLAAKLNAMLQA..........G..P. Pseudantechinus mimulus
 AY532667 ..V.........................V.TEEDLAAKLNAMLQA.............P. Murexia longicaudata
 EF028746 ..V.........................V.TEEDLAAKLNAMLQA.............P. Paramurexia rothschildi
 AY532677 ..V.........................V.TEEDLAAKLNAMLQA.............P. Dasyuroides byrnei
 EF028745 ..V..........I..............V.TEEDLAAKLNAMLQA.............P. Phascomurexia naso
 
 Macropus eugenii assembly         
 sacHar   MEILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE 
          ME+LQ YYTLVDRVPALLHHLTAIDYSS L  +   ++       VSEDPRLLVRVLR E
 macEug   MEVLQNYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPE  
 
 Monodelphis domestica assembly     TSSLVLDLQHSSGGEISG 
 sacHar   MEILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE  
          ME+LQ YYTLVDRVPALLHHLTAIDYSS L  +   ++       VSEDPRLLVRVLR E
 monDom   MEVLQNYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPE  
 
 Ornithorhynchus anatinus assembly
 sacHar    EILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE 
           ++L+ YY LVDRVPALL HL A+D SS L  +   SR        SEDPRLLVR L  E
 ornAna    DLLRDYYALVDRVPALLRHLAALDLSSVLSEEDLTSRLNAGLQAASEDPRLLVRRLEPE  
 
 Equus caballus assembly
 sacHar    EILQKYYTLVDRVPALLHHLTAIDYSSSLVLDLQHSRGGEVSGTVSEDPRLLVRVLRSE  
           E LQ YYTLVDRVPALLHHL ++D+SS +  D   ++       VSEDPRLLV V+RS+
 equCab    EALQDYYTLVDRVPALLHHLASMDFSSVVSEDDLVAKLNAGLQAVSEDPRLLVWVVRSK

It emerges from direct tblastn that the Sacrophilus individual sequenced was female. That is, ATRX is well represented but not ATRY (though the situation is somewhat confused due to additional paralogs). Marsupial XY are [http://www.ncbi.nlm.nih.gov/pubmed/11173870,12508115,16209912,17333539,18185981,9215558 quite different] from placentals: 
<blockquote>
"Many or most genes on the mammal Y chromosome evolved a testis-specific function after diverging from an X-borne copy with a general function in both sexes. In marsupial but not eutherian mammals, a testis-specific orthologue (ATRY) of the widely expressed X-borne ATRX gene lies on the Y chromosome. Since mutations in human ATRX cause sex reversal, it is possible that one function of ATRY in marsupials is testicular differentiation. We report here the isolation and sequencing of the tammar wallaby (Macropus eugenii) ATRY cDNA, and comparison of its sequence with that of tammar ATRX. The evolution of a testis-specific function for the ATRY protein distinct from the general role of ATRX in both sexes has been accompanied by sequence changes in many protein domains that would alter protein binding partners. A large open reading frame encodes a 1771 amino acid ATRY protein that has diverged extensively from ATRX. The conservation and loss of particular motifs identify those required for testicular function (ATRY) and function in other tissues (ATRX)."</blockquote>

== Reference sequences ==

These are organized both parsed into functional modules (signal peptide and spacer residues are dropped) as determined by global multiple alignment consistency and as intronated genes showing position and phase of intron breaks. Both sets are in phylogenetic order with respect to the canonical deuterostome tree.

=== RBP3 (IRBP) sequences parsed into structural modules ===

<pre>
>M1_homSap
LFQPSLVLDMAKVLLDNYCFPENLLGMQEAIQQAIKSHEILSISDPQTLASVLTAGVQSSLNDPRLVISYEPSTPEPPPQVPALTSLSEEELLAWLQRGLRHEVLEGNVGYLRVDSVPGQEVLSMMGEFLVAHVWGNLMGTSALVLDLRHCTGGQVSGIPYIISYLHPGNTILHVDTIYNRPSNTTTEIWTLPQVLGERYGADKDVVVLTSSQTRGVAEDIAHILKQMRRAIVVGERTGGGALDLRKLRIGESDFFFTVPVSRSLGPLGGGSQTWEGSGVLPCVGTPAEQALEKALAIL
>M1_bosTau
LFQPSLVLEMAQVLLDNYCFPENLMGMQGAIEQAIKSQEILSISDPQTLAHVLTAGVQSSLNDPRLVISYEPSTLEAPPRAPAVTNLTLEEIIAGLQDGLRHEILEGNVGYLRVDDIPGQEVMSKLRSFLVANVWRKLVNTSALVLDLRHCTGGHVSGIPYVISYLHPGSTVSHVDTVYDRPSNTTTEIWTLPEALGEKYSADKDVVVLTSSRTGGVAEDIAYILKQMRRAIVVGERTVGGALNLQKLRVGQSDFFLTVPVSRSLGPLGEGSQTWEGSGVLPCVGTPAEQALEKALAVL
>M1_monDom
IFQPSLVRDMAKILLDNYCFPENLMGMQEVIEQAIKSGEILDISDPQMLASVLTAGVQGALNDPRLVISFEPSIPETPQHVPKLANVTQEELLILLQQMIKYQVLEGNVGYLRVDYIPGQEVVEKVGEFLVNNIWKKLMGTSSLVLDLQHSSGGEISGIPFVISYLHQGDILLHVDTVYDRPSNTTTEIWTLPQVLGERYGGEKDMVVLTSHRTVGVAEDIAYILKKLRRAIVVGEQTLGGALDLRKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALGKALAIL
>M1_macEug
IFQPSLVLDMAKILLDNYCFPENLMGMQEAIEQAIKSGEILDISDPQMLASVLTAGVQGSLNDPRLVISYEPSPAEAPQQSPKLTSLTQEELLTLLQQMIKYQVLDGNVGYLRVDYIPGQEVVEKVGEFLVNDIWKKLMGTSSLVLDLQHSTEGEVSGIPFVISYLHEGDILLHVDTVYDRPSNTTTEIWTLPQVLGERYSGEKDLVILTSHRTVGVAEDIAYILKKMRRAIVVGEQTLGGALDLRKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKALAIL
>M1_sacHar
IFQPTLVLDMAKILLDNYCFPENLMGMQEAIEQAIKSGEILDISDPQMLANVLTAGVQGSLNDPRLVISYEPSTSEAPQHDPKFANATQEELLALFQKIIKYQVLEDNVGYLRVDYIPGRDMIEEVGEFLVNDIWKKVMETSSLVLDLQHSRGGEVSGIPFVISYLHQGDILLHVDTIYDRPSNTTTEIWTLPQVLGERYSGEKDIVVLTSHHTVGVAEDIAYILKKMRRAIVVGEQTQGGSLDLRKLRIGQSDFFITVPVARSLSPLGGGSQTWEGSGVVPCVGIPAEQALEKALAIL
>M1_ornAna
VSQPSMVLDVAKILLDNYCYPENLMGMQEAIEEAIQRGEILDIADPKRLASVLTAGVQGSLNDPRLVISYEPAPVAVSQQPPEPASLPAEQPLERLRPAVGSEVLEGNVGYLRVDRLPGREEIERVGAVLGRDIWEKLLGTSALVLDLRHSTGGHVSGIPFFISYFYPEGPALHVDTVYDRPSNATRQLWTLPRVLGARYAADKDVVVLTSRLTAGVAEDVAYILQQMRRAIVVGERTAGGPLVFRKLRVGLSDFFITVPVACSLGPLGGGGRSWEGSGVLPCVAVPADRALDEALDIL
>M1_galGal
IFQPTLVLDMAKVLLDNYCYPENLVGMQEAIEQAIKSGEILDISDPKMLANVLTAGVQGALNDPRLVISYEPSLHAAPKQEAETYPTREQLLSLIEHVVIYDKLEGNVGYLRIDYIIGQEVVEKVGAFLVDKVWKTLINTSALVIDLRYSTGGQISGIPFIISYLHEADKMLHVETVYNRPSNTTTEIWTLPKVLGERYSKDKDVIVLISHHTTGVAEDVAYILKHMNRAITLGEKTAGGSLDIQKLRIGPSNFYMMVPVSRSVSPLSGGGQSWEVSGVMPCVASEAEQALKKSLDIL
>M1_taeGut
IFQPTLVLDMAKVLLDNYCYPENLVGMQEAIEQAIKSGEILDISDPKMLANVLTAGVQGALNDPRLVISYEPLPHSGPKQEAEGSPTREQLLSLIEHVIMYDKLEGNVGYLRIDYIIGEEVVQKVGAFLVDKVWKTLIETSALVIDLRHSTGGQISGLPFIISYLHEQDKILHVETVYNRPSNTTTEIWTLPKVLGERYSKDKDVIVLISHHTTGVAEDVAYILKHMNRAITVGEKTAGGSLDIQKLRIGPSNFYMMVPVSRSVSPLSGGGQSWEVSGVMPCVATEAEQALQKSLDIL
>M1_anoCar
VLQSTLVLDMAKLLLDNYCLPENLVGMREAIEQAIKNGEVLDISDPKLLATVLTAGVQGALNDPRLVISYEPTAPAAPKQRMETSLTPEQLLSLIQHTVKYEVLDDNVGYLRIDYIMGQDIVQKIGSFLVEKVWKTLLGTSALILDLRYTTGGDVSGIPFIISYLYNGDKVLHVDTVYNRPSNTTVEILTLPKVLGVRYSKDKDVILLISKYTTGVAENVAYILKHMHRTIIVGEKSAGGSLDTQKMQIGNSQFYMTVPLSCSVSPLSGSGQSWEISGVTPCVVISAEQALDKALAIL
>M1_xenLae
LFQPSLVMDMAKVLLDNYCFPENLVGMQETIEQAVKGGEILHISDPDTLANVFTSGVQGYLNDPRLVVSYEPNYSGPQTEQSLELTPEQLKFLINHSVKYDILPGNIGYLRIDFIIGQDVVQKVGPHLVNNIWKKLMPTSALILDLRYSTQGEVSGIPFVVSYLCDSEIHIDSIYNRPSNTTTDLWTLPELMGERYGKVKDVVVLTSKYTKGVAEDASYILKHMNRAIVVGEKTAGGSLDTQKIKIGQSDFYITVPVSRSLSPLTGQSWEVSGVSPCVVVNAKDALDKAQAIL
>M1_xenTro
VFQPSLVMDMAKVLLDNYCFPENLVGMQETIEQAMKSGEILHISDPETLANVFTSGVQGFLNDPRLVVSYEPNYSGPRKEQSPEPTLEQLKFLLDHSVTYDLLPGNIGYLRIDFIIGQDVVQKVGPLLVNNIWKKLMPSSALILDLRYSTQGKVSGIPFVVSYLTDPQIHIDSIYNRPSNTTTDLWTLSELMGERYGKDKDVVVLTSKYTEGIAEGAAYILKHMSRAIVVGEKTAGGSLDIQKIKIGQSEFYITVPVSRSISPLTGQSWEVAGVFPCVVVNANNALNKAQGIL
>M1_tetNig
AFPPSLIADMAKIVLDNYCSPEKLAGMKEAIKAAGTNTEVLNIPDGESLARVLSAGVQGTVSDPRLMVSFQPNYVPAGPHKMPPLPPEHLVAVLQTSVKLDILEGNTGYLRIDHILGEEVADKVGPALIDLIWNKILPTSALIFDLRYTSSGDISGIPYIVSYFTQAEPVVHIDSVYDRPSNTTTKLLSLPNLLGQRYGVSKPLIVLTSKNTKGIAEDVAYCLKNLKRATIVGEKTAGGSLKLDTFKVGDTDFYITVPTAKSINPITGSSWEIRGVTPHVEVNAEDALATAIKIV
>M1_takRub
AFPPSLITDMAKIVLDNYCSPEKLAGMKEAIEAAGTNTEVLNIPDGESLARVLSAGVQGTVSDSRLMVSYQPDYVPAVPPKMPPLPPEHLVAVLQTSIKLDLLEGNTGYLRIDHIIGEDVAEKVGPSLIDLIWNKILPTSALIFDLRYTSSGEISGIPYIVSYFTQAEPVVHIDSVYDRPSNTTTKLFSLSNLLGERYGITKPLIILTSKNTKGIAEDVAYCLKNLKRATIVGERTAGGSVKLDNFKVGSTDFYITVPTAKSINPVTGSSWEITGVKPDVEVNAEDALATAIKIV
>M1_gasAcu
GFAPNVIIDMAKIVIDNYCSPEKLAGMKEAIEAAGSNTEVLSIPDAETLANVLSAGVQTTVSDPRLMISYEPNYVPVVPPKMPPLPPDQVIAVLQTSIKLDILEGNIGYLRIDHILGEDVAEKVGPLLLDLVWNKILPTSALIFDLRYTSSGDISGIPYIVSYFTEAGTPIHIDSIYDRPSNTTTKLFSMSTLLGERYSTSKPLIILTSKNTKGIAEDVAYCLQNLKRATIVGEKTAGGSVKVDKIQVRDTGFYVTVPTAKSVNPITGSTWEVTGVTPNVEVNAEDALATAIKIV
>M1_oryLat
SFPPSLITDLAKIVMDNYCSPEKLSGMKEDIATAGANTDVLNIPDGEALAKVLTDGVQTTVSDPRLRVSYEPNYVPVVPPQLPPEQLIAVLQTSIKLDILEGNIGYLRIDSIIGEEVAEKVGPLLLELVWSKILPTSALIFDLRYTSSGDITGIPYIISYLTDAKSEIHIDTIYDRPLNTTTKLLSMQSTLGQTYGGTKPLLVLTSKNTKDIAEDVAYCLKNLKRATIVGEKTAGGSAKIKKFRVGDTDFYVTLPTAKSINPITGSSWEVTGVKPNVEVNAEEALATALKII
>M1_danRer
NFSPTLIADMAKIFMDNYCSPEKLTGMEEAIDAASSNTEILSISDPTMLANVLTDGVKKTISDSRVKVTYEPDLILAAPPAMPDIPLEHLAAMIKGTVKVEILEGNIGYLKIQHIIGEEMAQKVGPLLLEYIWDKILPTSAMILDFRSTVTGELSGIPYIVSYFTDPEPLIHIDSVYDRTADLTIELWSMPTLLGKRYGTSKPLIILTSKDTLGIAEDVAYCLKNLKRATIVGENTAGGTVKMSKMKVGDTDFYVTVPVAKSINPITGKSWEINGVAPDVDVAAEDALDAAIAII
>M1_petMar
KFDTAVVLHLAKVLLDNYCIPENLVGMDEAIQRAVDNGELLGVSDPESAASALTEGIQAALNDPRIAVSYVAPPHTFEELLATIPQKTSFAVLDGNVGYLRADEIISEATIKKLGPVIVQRIWNRLVDTDTFVLDLRYNSHGDITGLPYLVSCFCEPRPVVHLDTVYYRPTNESKEIWSLPDLQGARFAKHKDVFVLVSANTEGVAENVAYVLKHLHRATVIGEQTAGGSLEVERFRLGDSRFFVTVPTARSEPADRSWGVFPCVSAPSERALDKALEIL
>M1x_takRu
FYQHTLVLEMAKLLLENYCIPENLVGMQEAIQRAIKSREILQISDRKTLATVLTVGVQGALNDPRLSVSYEPSFSPLPLQALSSLPVEQQLRLLRNSIKLDILDSDVGYLRIDRIIDEETLLKFGPLLRENVWDKAAQTSSLILDLRFSTAGGWSGIPSIVSYFTEPHSLVHIDTVYDRPSNTTTELWTMSSVRGKTFGGKKDMIVLIGRRTAGAAEAVAYTLKHLNRAIVVGERSAGGSLKVRKFRIAESDFYITMPVARSVSPITGKSWEVSGISPTVNVAAREALAKAQTFL
>M1x_danRe
SFQSALVLDMAKILLDNYCFPENLIGMQEAIQQAINSGEILHISDRKTLASVLTAGVQGALNDPRLTVSYEPNYTLITPPALHSLPTEQLIRLIRSTVKLEVMDNNIGYLRIDRIIGQETVVKLGRLLHNNIWKKVAHTSAMIFDLRFSTAGELSGLPYIVSYFSDSDPLLHIDTIYERPTNITRELWTLPTLLGERFGKRKDLIVLISKRTIGAAEGVAYILKHLKRAVIIGERSAGGSVRVDKLKIGDSGFYITVPVARSVNPVTGQSWEVSGVAPSVTVNPKESIAKAKSLI

>M2_homSap
SALPGVVHCLQEVLKDYYTLVDRVPTLLQHLASMDFSTVVSEEDLVTKLNAGLQAASEDPRLLVRAIGPTETPSWPAPDAAAEDSPGVAPELPEDEAIRQALVDSVFQVSVLPGNVGYLRFDSFADASVLGVLAPYVLRQVWEPLQDTEHLIMDLRHNPGGPSSAVPLLLSYFQGPEAGPVHLFTTYDRRTNITQEHFSHMELPGPRYSTQRGVYLLTSHRTATAAEEFAFLMQSLGWATLVGEITAGNLLHTRTVPLLDTPEGSLALTVPVLTFIDNHGEAWLGGGVVPDAIVLAEEALDKAQEVL
>M2_bosTau
RALPGVIQRLQEALREYYTLVDRVPALLSHLAAMDLSSVVSEDDLVTKLNAGLQAVSEDPRLQVQVVRPKEASSGPEEEAEEPPEAVPEVPEDEAVRRALVDSVFQVSVLPGNVGYLRFDSFADASVLEVLGPYILHQVWEPLQDTEHLIMDLRQNPGGPSSAVPLLLSYFQSPDASPVRLFSTYDRRTNITREHFSQTELLGRPYGTQRGVYLLTSHRTATAAEELAFLMQSLGWATLVGEITAGSLLHTHTVSLLETPEGGLALTVPVLTFIDNHGECWLGGGVVPDAIVLAEEALDRAQEVL
>M2_monDom
RARPGAIQRLMEVLQNYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPEEATMGSEASEEEDATPAANSLPEDESQRQALVDSVFQVSVLPGNVGYLRFDEFADSSVLGTLAPYVIRQVWEPLQDTNHLIMDLRYNPGGPSSAVPLLLSYFQDPAAGPIRLFTTYDRQTNQTQEHLSRAELLGKPYGAQRGVYLLTSHHTATAAEEFAFLMQSLGRATLVGEITAGSLMHTRTFPLLQPPNGNLVLTVPILTFIDNNGECWLGGGVVPDAIVLAEEALDKAKEVL
>M2_macEug
RARPAAIQRLMEVLQNYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPEEATAGAESREEAATAAPVPLPDGESQRQALVNSVFQVSVLPGNVGYLRFDEFADSSVLGALAPYVLQQVWEPLQDTDHLIMDLRYNPGGPSSAVPLLLSYFQDPAAGPVRLFATYDRQTNQTREYRSQAELLGKPYGAQRGVYLLTSHHTATAAEEFAFLMQSLGRATLVGEITAGNLMHTRTFSLLQPPDGSLVLTVPILTFIDNHGECWLGGGVVPDAIVLAEEALDKAKEVI
>M2_sacHar
RARPGAIQRLMEILQKYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAMLQAVSEDPRLLVRVLRPEEATVEAEPGEESATPASVSLPESDAERQALIDSVFQVSVLPGNVGYLRFDEFADNSVLGTLAPYVLRQVWEPLQDTDHLIMDLRYNPGGPSSAVPLLLSYFQDPSAGPVRLFATYDRQTNQTQEYRSRAELLGKPYGAERGVYLLTSYHTATAAEEFAFLMQSLGRATLVGEITAGSLMHTRTFPLLQPPNGSLVLTVPTLTFIDNHGECWLGGGVVPDAIVLAEEALDKAKEVL 
>M2_ornAna
GAVPGAVAHLADLLRDYYALVDRVPALLRHLAALDLSSVLSEEDLTSRLNAGLQAASEDPRLLVRRLEPEEAERGPPRKEEEQKEEEEEDQPSPGASILPGDGSSLFRVSVLPGNVGYLCFDEFPEASALERLGPLLGRRVWEPLEATDHLMVDLRNNPGGPSSAVPLLLSYFQDPAAGPIRLFTTYNRPADVTREYASRAGALEKPYGARRGVYLLTSHRTATAAEEFAYLMQALGRATLVGEITAGRLLHSRTFPLLRPPWEGLVLTVPFLTLFDPHGEGWLGGGVVPDAIVLAEEALEKAGEVL
>M2_galGal
RAVPGTLSRLTDILKDYYSLVERVPVLLRHLTTSDFSSVQSAEDLATKLNTEMQTLSEDPRLLVRTMMPGEAAAPPAEMPIAMAANLPDNEQLLHALVDTVFKVSVLPGNVGYMRFDEFADASVLVKLGPYIVKKVWEPLQNTENLIMDLRYNPGGPSSSAVPMLISYFQDPTAGPVHLFTTYDRRTNHTQEHNSQAELLAQPYGAQRGIYVLTSRHTATAAEEFAYLMQSLGRATLIGEITAGSLSHTCTFPLVQPEQGITRGLTITVPVITFIDNHGESWMGGGVVPDAIVLAEDALEKAEEVL
>M2_taeGut
RAVPGTISHLKNILKDYYSLVERVPALLRRLTTSDFSSVQSSEDLATKLNTELQALSDDPRLMVRVMMPGEAADSPAEKPVGMAADLPDNEQLLHALVDTVFKVSVLPGNVGYMRFDEFADASVLVKLGPYLVHKVWEPLQNTENLIMDLRYNLGGPSSSAVPVLLSYFQDPAAGPVHLFTTYDRRTNHTQEHNSQAELLGQSYGAKRGVYLLTSHHTATAAEEFAYLMQSLGRATLIGEITAGSLSHTRTFPLLQPGPGITRGLTITVPVITFIDNHGESWMGGGVVPDAIVLAEDALEKAEEVL
>M2_anoCar
KAIPNSMSYLVDIIKNNYSMLEQVPVLLQHLSTFDYSSVLSVKDLASKLNAELQTISEDPRLFLRVPASDEAVTSQTDEKVAMASDLPNNEQLMKALVMTVFKVSVLPGNVGYMRFDEFGDATVLVKLGPYLLQHVWEPLQATDYLIIDLRYNIGGPSSSAVPVLLSYFQDPSAGPVHFFTTYNRLTNQTQAYSSSAEMVGKPYGARRGVYLLTSHNTATAAEEFAYLMQTLGRATLVGEITAGSLSHTHTFCILELGGGCGLLINVPVITLIDNHGEYWLGGGVVPDSIVLADEALEKAREVL
>M2_xenTro
SSITHILLQLSEILVNNYAFSERIPTLLQHLPNLDYSSVISEEDITAKLNYELQSLTEDPRLVLKSKTDSLVMPEDSTQVENLPDDEATLQALVNTVFKVSILPGNIGYLRFDEFADVSVLAKLGPYIVNTVWDPITVTENLIIDLRYNIGGSSTSIPLLLSYFQEPENRIHLFTIYNRQQNSTNEVYSLPKVLGKPYGSKKGVYVLTSHETATAAEEFAYLMQSLSRATIIGEITSGNLMHSKAFPLDGTRLSVTVPIMNFIDNNGDYWLGGGVVPDAIVLADEALDKAKEII
>M2_xenLae
SSVTHVLHQLCDILANNYAFSERIPTLLQHLPNLDYSTVISEEDIAAKLNYELQSLTEDPRLVLKSKTDTLVMPGDSIQAENIPEDEAMLQALVNTVFKVSILPGNIGYLRFDQFADVSVIAKLAPFIVNTVWEPITITENLIIDLRYNVGGSSTAVPLLLSYFLDPETKIHLFTLHNRQQNSTDEVYSHPKVLGKPYGSKKGVYVLTSHQTATAAEEFAYLMQSLSRATIIGEITSGNLMHSKVFPFDGTQLSVTVPIINFIDSNGDYWLGGGVVPDAIVLADEALDKAKEII
>M2_petMar
GVARKAVEAAGELLLSSYTFVERASAIADHLSWSEYGSVVSVEDLTSKLTQDLQSVAEDPRLVVSNREPEWVGAADPPGPPAPLPDDEQMLEAIVDSAFKVEVLEGNIGYLRFDEFGDASAVMKLRKQLVSKVWERIHPTDDVIIDLRYNLGGSSTAIPIVLSYFQDVAPVHFYTVYDRLRNVTAEFHTVSNLTSQLYGSKKGVYLLTSQHTATAAEEFTYLMQSLNRATIVGEITSGRLAHSLAFRLSDTGLYMTVPIVNFIDNNDEYWLGGGVVPDAIVLAENALDAAKEII

>M2x_takRu
SRIPKVLQIVLDIIGRFYAFADRVQALLQQLESADLFSVVSEEDLAARLNHDLQTASEDPRLIIRHKRDNIPRAEEEPELHAANDHDGELVEGFTVQVLPHNTGYLRLDRFVRCSEGDKLEEIVAEKVWGPLKDTQNLIIDLRHNTGGSSTSVALLLSYLRDPLPKRHFFTIYDSVQNTTTEYGSRPHIPGPSYGSERGVYVLTSHYTAGAAEEFAYLIQSLHFGTVVGEITSGTLMHSKTFQVEGTDIFITVPFINFLDNNGEYWLGGGVVPDAIVLAEEALEHVNRTA     
>M2x_danRe
KTIPKAVRRVSDIIKRYYSFKDKIPALLNQLAKADYFTVVSEEDLAGKLNHEMQSVFEDPRLLIKATQVLTDDASSEDRSSSDDLTDPLFKLEMISGNNGYLRFDRFPTPEVLLRLEDHIKKKIWQPVQETENLVIDLRFNTGGSTEALPILLSYMFDTSSSTYLFSIYDSIKNTTFDFHTLNNISGPSYGSTKGVYVLTSYYTAEAGEEFAYLMQSLHRGTVIGEITSGMLLHSKTFQIEQTSLAITVPIINFIDVNGECWLGGGVVPDAIVLAEEALERAHEII

>M3_homSap
QSLGALVEGTGHLLEAHYARPEVVGQTSALLRAKLAQGAYRTAVDLESLASQLTADLQEVSGDHRLLVFHSPGELVVEEAPPPPPAVPSPEELTYLIEALFKTEVLPGQLGYLRFDAMAELETVKAVGPQLVRLVWQQLVDTAALVIDLRYNPGSYSTAIPLLCSYFFEAEPRQHLYSVFDRATSKVTEVWTLPQVAGQRYGSHKDLYILMSHTSGSAAEAFAHTMQDLQRATVIGEPTAGGALSVGIYQVGSSPLYASMPTQMAMSATTGKAWDLAGVEPDITVPMSEALSIAQDIV
>M3_bosTau
RSLGELVEGTGRLLEAHYARPEVVGQMGALLRAKLAQGAYRTAVDLESLASQLTADLQEMSGDHRLLVFHSPGEMVAEEAPPPPPVVPSPEELSYLIEALFKTEVLPGQLGYLRFDAMAELETVKAVGPQLVQLVWQKLVDTAALVVDLRYNPGSYSTAVPLLCSYFFEAEPRRHLYSVFDRATSRVTEVWTLPHVTGQRYGSHKDLYVLVSHTSGSAAEAFAHTMQDLQRATIIGEPTAGGALSVGIYQVGSSALYASMPTQMAMSASTGEAWDLAGVEPDITVPMSVALSTARDIV 
>M3_monDom
QRLGALVEGTGHLLEAHYALPEVVGQASALLKAKLEHGTYRTAVDFESLASQLTSDLQEVSGDHRLHVFHSPGEPVSEELTPPQKGVPSPEELTYLIEALFKTEVLPGQLGYLRFDMMAEAETVRAIAPQLVELVWEKLVHTEALVVDLRYNPGGYSTAVPLLCSYFFEAEPRRHLYTIFDRAASQLTEVWTLPQVAGERYGSQKDLYILISHTSGSAAEAFVHTMKDQHRATVIGEPTGGGALSVGIYQVENSPLYASMPTQVAISPVTGKAWDMAGVEPDVSVLSSEALMTTQGIV 
>M3_macEug
QRLGALVEDAGHLLEAHYALPEVVGQASALLRARLVHGTYRTAVDFESLASQLTSDLQEVSGDHRVHVFHSPGELIPEELSPPQNVVPSPEELTYLIEALFKTEVLPGQLGYLRFDMMAEAETVRAIGPQLIELVWEKLVNTEALVVDLRYNPGSYSTSVPLLCSYFFEAEPRKHLYTIFDRAASQATEVWTLPQVAGERYGSQKDLYILISHTSGSAAEAFAHAMKDLRRATVIGEPTAGGALSVGIYQVSNSPLYASMPTQVAISPVTGKAWDIAGVEPDVSVPAREALITTQGIV
>M3_sacHar
QRLGALVEGTGHLVEAHYALPEVVGQASAFLRATLAHGTYRTAVDFESLASQLTSDLQEVSGDHRLHVFHSPGEPVPEESSPPHKGVPSPEELTYLIEALFKTDVLPQLGYLRFDMMAEVETVRAIGPQLVELVWEKLVNTEALVVDLRYNPGSYSTTVPLLCSYFFEAEPRKHLYTVFDRATSQFTEVWTLPQVTGERYGSQKDLYILISHTSGSAAEAFAHIMKDLQRATVIGEPTAGGALSVGIYQVGDSPLYVSMPTQVALSPVTGKAWDMAGVEPDVSVLANEALITAQGIV
>M3_taeGut
KNMGVLLEGTGQLLEDHYAIPEVAAKASAMLSTKRAQGGYRSAIDSETLASQLTSDLQEASGDHRLHVFHSHVEPTPEEQLPNVIPSPEELSYIIEALFKIEVLPGNLGYLRFDMMAEAETVKAIGPQLLQMVWNKLVDTDAMIIDMRYNTGGYSTAIPILCSYFFDPEPRKHLYTVFDRSTSRSTEVWTLPQLAGKRYGSLKDIYILTSHMSGSAAEAFTRSMKDLHRATVVGEPTVGGSLSVGIYRVGNSSLYASIPSQVVLSPVTGKVWSVSGVEPHITIQASEAMAAAQHIA 
>M3_galGal
RKMGILLESTGQLLEAHYAIPEVAEKASVMLSTKRVQGGYRSAVDFETLASQLTSDLQEASGDHRLHVFHSHVEPTPEEQLPNMIPSPEELSYIIEALFKIEVLPGNLGYLRFDMMAEAETVKAIGPQLVQMVWNKLVDTDAMIIDMRYNTGGYSTAVPILCSYFFEPEPRQHLYTVFDRSTSRSTEVWTLPKVTGKRYGSLKDIYILTSHMSGSAAEAFTRSMKDLHRATVIGEPTVGGSLSVGIYRVGNSSLYRSIPSQVVLSPVTGKVWSVSGAEPHITIQASEALAAAKHIA 
>M3_anoCar
KGMGSLIERVGQLLEAHYAIPEMARRVSSMLNSKLAQGGYRTAVDFETLASQLTNDLQETSGDHQLHVFHSHVEPSLEEQSPFKTLTPEELNFIIEALFKVDVLPGNVGYLRFDMMAEFESVKTIEPQILHMVWEKLVETSAMIVDMRYNTGSYSTAVPMFCSYFFDAEPQQHLYTIIDRSTSQSTEVWTSSQVSGKRYGSTKDLYILISHASGSAAEAFTRSLKDLHRATVIGEPTVGGSLSASIYNIGSTPLYASIPSQIVLSPVSGKVWSLSGIQPHVTTQSNEALASAQNII
>M3_xenTro
PSVFALVEGTGHLLEVHYAIPEVAYKVSSVLQNKWSEGGYRSVVDLESLASQLTSEMQENSGDHRLHVFYSDTEPEILEDQPPKIPSAEELNYIIDALFKIEVLQGNVGYLRFDMMADTEIIKAIGPQLVSLVWNKLVETNSLIIDMRYNTGGYSTAIPIFCSYFFDPEPLQHLYTVYDRSTSSGTDIWTLPEVVGERYGSTKDIYILTSHMTGSAAEVFTRSMKELNRATIIGEPTSGVSLSVGMYKVGESNLYVSIPNQVVISSVTGKVWSVSGVEPHVIAQASEAMNVAHHII
>M3_xenLae
PSIFPLVKGTGHLLEVHYAIPEVAYKVSSVLQNKWSEGGYRSVVDLESLASLLTSEMQENSGDHRLHVFYSDTEPEILEDQPPKIPSPEELNYIIDALFKIEVLPGNVGYLRFDMMADTEIIKAIGPQLVSLVWNKLVETNSLIIDMRYNTGGYSTAIPIFCSYFFDPEPLQHLYTVYDRSTSTGKDIWTLPEVFGERYGSTKDIYILTSHMTGSAAEVFTRSLKDLNRATLIGEPTSGVSLSVGMYKVGDSNLYVTIPNQVVISSVTGKVWSVSGVEPHVIIQANEAMNIAHRII
>M3_petMar
AKMASLLELAGALVEGYYAMLSDGENATAEILLKYREGWYRSVVDYEALASQLTSDLHEIWGDHRLHAFYSDLQIERMDEDKTPSVPSPEELSVLIDTVFKVDILANNVGYLRFDMMTDAEVLKHVGPQLVEKVWNKISSTRSLVIDVRYNMGGYSTSIPILCSYFFDASPPRHLYTVFDRPSRSSTQVFTVPRVLGQRYGASKDVYILTSHMTGSAGEILTRVMSDLKRATVIGEPTAGGSLSTGTYRIGDSRLYVFIPNQAGVSPSGGRTWSVAGVEPHVQTKASEALQSALRMV

>M3x_takRu
QGLRSLIGRTGELLEKHYAIQEVAQKVGEVLLSKWAEGLYRSVVDLESLASQLTADLQEASGDHRLHVFRCDVELESLHGVPKIAAVEEAGFVIDALFKSELLPRNVGYLRFDTMADIEAAKGAAPRLVKSVWNKLVDTDSLIIDMRYNAGGSSTAVPLWCSYFVDGEPLQHLYTVYDRTTKTRVEVMTLPEVSGQRYDPGKDVYILTSHMTGSAAEAFVRAMRDLNRVTIVGEPTAGGSLSSATYQIGESVLYASIPNQVVTSAATGKLWSISGVEPDVFAQARDALPVAQRII
>M3x_danRr
KNIQGLVQEAGDLLEKHYSVPEVAAKVSRLLQSKLTEGLYRSVVDYESLASQLTSDLQETSGDQRLHIFYCETEPETLHDTPKIPSPEEAGFIVEALFKVDVMSGNIGYLRFDMMEDIKVLQAINPEFLKVVWNKLVNTDMLIIDVRYNTGGYSTAIPLLCTYFFDAQPLTHIYTLFDRSTATVTKVTTLPDVLGQKYSSQKDVYILTSHITGSAAEAFTRTMKDLKRATVIGEPTIGGALSSGTYQIGNSILYASIPNQAVLNAVTGKPWSISGVEPHIVAQASDALIVAQKII

>M4_homSap
AKVPTVLQTAGKLVADNYASAELGAKMATKLSGLQSRYSRVTSEVALAEILGADLQMLSGDPHLKAAHIPENAKDRIPGIVPMQIPSPEVFEELIKFSFHTNVLEDNIGYLRFDMFGDGELLTQVSRLLVEHIWKKIMHTDAMRIIDMFNIGGPTSSIPILCSYFFDEGPPVLLDKIYSRPDDSVSELWTHAQVVGERYGSKKSMVILTSSVTAGTAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDTNLYLTIPTARSVGASDGSSWEGVGVTPHVVVPAEEALARAKEML
>M4_bosTau
AKVPTVLQTAGKLVADNYASPELGVKMAAELSGLQSRYARVTSEAALAELLQADLQVLSGDPHLKTAHIPEDAKDRIPGIVPMQIPSPEVFEDLIKFSFHTNVLEGNVGYLRFDMFGDCELLTQVSELLVEHVWKKIVHTDALIVDMRFNIGGPTSSISALCSYFFDEGPPILLDKIYNRPNNSVSELWTLSQLEGERYGSKKSMVILTSTLTAGAAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDTDLYLTIPTARSVGAADGSSWEGVGVVPDVAVPAEAALTRAQEML
>M4_monDom
AKVPTILQTAGKLVADNYASLEVGSRVASKLAKLQTQYRQVTSEGELADMLGADLQTLSGDRHLKTAHIPEDAKDRIPGIVPMQLPSPEAFEDLIKFSFHTNVFEGNIGYLRFDMFGDCELLTQVSDLLVEHVWKKVVHTDGMIIDMRFNIGGPTSSISALCSYFFDEGQEVLLDQIYNRPNDSISEIWTQSQVAGERYGSKKSVIILTSSMTAGAAEEFVYVMQRLGRALVIGEVTSGGCQPPQTYHVDDTDLYITIPTARSVGSGDKPSWEGVGVAPHVEVPADQALSKAKEMF 
>M4_macEug lower case: sacHar fix
SKVPTILQTAGKLVADNYASPEVGSRVAAKLARLQTQYRQVTSEGELADMLGADLQTLSGDSHLKTAHIPEDSKDRIPGIVPMQlpspeafedlikfsFHTNVFEGNIGYLRFDMFGDCELLTQVSDLLVEHVWKKVVHTDGMIIDMRFNIGGPTSSISALCSYFFDEGQKVLLDRIYNRPNDSIVEIWTQPHVTGERYGSKKSVIILTSSTTAGAAEEFVYIMQGLGRALVIGEVTSGGCQPPQTYHVDDTDLYITIPTAQSVGSGDRPSWEGIGVTPHVEVPADQALSKAKEMF
>M4_sacHar
AKVPTILQTAGKLVADNYASPEVGSRVAAKLASLQIQYGKVTSEGELADMLGADLQTLSGDRHLKTAHIPEDAKDRIPGIVPMQLPSPEAFEDLIKFSFHTNVFEGNIGYLRFDMFGDCELLIQVSDLLVEHVWKKVMHTDGMIIDMRFNIGGPTSSISAMCSYFFDEGQGVLLDRIYNRPNDSISEIWTQPQVIGERYGSKKTVVILTSSMTAGAAEEFVYIMQRLGRALVIGEVTSGGCQPPQTYHVDDTDLYITIPTTRSVVSGDKSSWEGVGVVPHMEVPADQALSKAKEMF
>M4_ornAna
SKVPTVLRTAAKLVADNYAFRETGAGVAAQMGGLQARCGRVTSEGALAEVLGAHLRALSGDPHLQMVYIPEDAKDRIPGVVPMQIPSAETFEDLIKFSFHTSVMEGNIGYLRFDMFGDCELLTQVSELMVEHVWKKIVHTDGLIIDMRNIGGPTSSISALCSYFFDEDHPVLLDKIYNRPNDSISEIWTHSHIAGERYGSRKSVVILTSNMTAGAAEEFVSIMKRLGRALVVGEVTGGGCHPPQTYHVDDTHLYITIPTSRSVGSEDGSSWEGVGVTPHLVVPADVALSRAKDLF 
>M4_taeGut
AQVPQILQTVGKLVADNYAFVNTGTVIASNLTKNIHKDNYKRINTEEDLAGKVTAILQALSDDKHLKLLYIPEHAKDSIPGIMPKQIPPPEVFEDLIKFSFHTNVFENNIGYLRFDMFGDSELLTQLSDLMIEHVWKKIFHTDALIIDLRYNIGGSTTPIAILCSYFFDEGHPVLLDRVYDRPSDSVKEIWTQPQLKGERYGSQKGLVILTSAVTAGAAEEFVYIMKRLSRALIIGEQTSGGCHSPQTYQVDETNFYVVIPTSRSVTSADSTSWEGKGVSPHIETPAETALIKAKEML 
>M4_galGal
TQVPQIVQTVGKLVAENYAFVDIGTDIASNLTKSVNKENYKRINSEKELARKLTAILQALSDDEHLKILYIPEHAKDSIPGILPKQIPSPEVFEDLIKFSFHTNVFENNIGYLRFDMFGDCELLTQVSDLLVEHVWKKIVHTDALIIDMRYNIGGYTNSIPILCSYFFDEGHQVLLDKVYDRPSDSVKEIWTQPQLRGERYGSQKGLIILTSAVTAGAAEEFVFIMKRLGRALIIGEQTSGGSHSPQTYQVDDTNFYIIIPTARSVISAESASWEGKGVPPHMETPAVTALIKAKEVL
>M4_anoCar
TKLPSVLNTIGKLVADNYAFADIGATVAAKFADYAKKGTYRKINSEIELSGKLAADLKALSGDRHLMISHIPERSKGRILGLVPMQQIPPPEILEDLIKFSLHTNVFENNIGYLRFDMFGDCELMSQVSELLVQHVWNKIVNTDALIIDMRYNVGGPACSVPLLCSYFFDEGHPILLDKVYNRPNDTTSNIWTVSKLAGKRYGLNKGLIILTSSVTSGAAEEFAHIMKRLGRAFIIGQKTSGGCHPPQTFHVDGTNLYITTPVSRSVFSVNDSWEGVGVSPHLDVSTDVALIKAKEML
>M4_xenLae
TKIPTVIQTAAKLVADNYAFADTGANVASKFIALVDKIDYKMIKSEVELAEKINDDLQSLSKDFHLKAVYIPENSKDRIPGVVPMQIPSPELFEELIKFSFHTDVFEKNIGYIRFDMFADSDLLNQVSDLLVEHVWKKVVDQDALIIDMRFNIGGPTSSIPIFCSYFFDEGTPVLLDKIYSRTSNAMTDIWTLPDLVGKTFGSKKPLIILTSSLTEGAAEEFVYIMKRLGRAYVVGEVTSGGCHPPQTYHVDDTHLYLTIPTSRSASAEPGESWEGKGVLPDLEISSETALLKAKEIL
>M4_xenTro
TKIPSVIQTAGKLVADNYAFADTGADVASKLIALVDKINYKMIKSEVELAEKLNYDLQSLSKDVHLKAVYIPENSKDRIPGVVPMQIPSPEMFEDLIKFSFHTDVFEKNLGYIRFDMFADSDLLNQVSDLLVEHVWKKVVNQDALIIDMRFNIGGPTSSIPTFCSYFFDEGTPVLLDKIYSRTTNAITDVWTLPHLVGNAFGSKKPVIILTSSLTEGAAEEFVYIMKRLGRAYVIGEVTSGGCHPPQTYHVDDTHLYLTIPTSRSASAKPGESWEGKGVLPDLEITSETALMKAKEIL
>M4_tetNig
AQIPAIIEGTAALVANNYAFEATGADVAKELRELQANGQYSSVVSKESLEAALSADLQRLSGDKSLKTTPNTPVLPPMDYTPEMYIELIKVSFHTDVFENNIGYLRFDMFGDFEEVKAIAQIIVEHVWNKVVNTDALILDLRNNVGGPTTAIAGFCSYFFDADKQNRVGQAVRQASGTTTELLTLSELTGVRYGSKKSLIILTSGATAGAAEEFVYIMKKLGRAMIVGETTAGASHPPQTFRVGETDVFLLIPTVHSDTGAGPAWEGAGIAPHIPASAEAALGTARAIL   
>M4_takRub
AQIPAIIEGAATLIAKNYAFEATGADVATKLRELLAKGQYNSVVSSESLEVALSADLQRLSGDKSLKATQNAPVLPPMDYSPEMYIELIKVSFHTDVFENNIGYLRFDMFGDFEEVKAIAQIIVEHVWNKVVNTDALILDLRNNVGGPTTAIAGFCSYFFDADKLIVLDKLHDRPSGTTTELLTLPELTGVRYGSKKSLIILTSGATAGAAEEFVYIMKKLGRAMIVGETTAGASHPPQVFSVGEIGIFLSIPTVHSDTAAGPAWEGTGITPHIPVSAEAALGTAKGIL   
>M4_gasAcu
NRVPAIIEGSATLIADNYAFEDIGAAVAEKLKGLLANGEYSKVVSKDSLEMKLSADLRTLSGDKSLKTTSNVPALPPMNYSPEMYIELIKVSFHTDVFEDNIGYLRFDMFGDFEEVKAIAQIIVEHVWNKVVNTDAMIVDLRNNIGGPTTAIAGFCSYFFDSDKQIVLDRLYDRPSGTTTELRTLPELTGTRYGSKKSLVMLTSRATAGAAEEFVYIMKKLGRAMIVGETTAGTSHPPKTFRVGETDIFLSIPTVHSDTAAGPAWEGAGVAPHIPVPADAALETAKGIF
>M4_oryLat
LQVPAIIEESATLVANNYAFESTAADVAEKLKGHLANGDYNMVVSKESLEAKLSADLQSLSGDKSLTVSSNTGAPPPMEYTPEMYIELIKISFHTDVFENNIGYLRFDMFGDFEEVKAIAQVIVEHVWNKVLHTDAMIIDLRNNVGGPTTAIAGFCSYFFDGDKQILLDKLYDRSTGTTTDLLTLGELTGERYGSKKSLIILASRATAGAAEEFVYIMKRLGRAMIVGETTAGASHPPKVFQVGESDIFLSIPTVHSDTSAGPGWEGAGVAPHIPVAAGAALETAKAIL   
>M4_danRer
AEIPALAQAAATLIADNYAFPSIGEHVAEKLEAVVAGGEYNLISTKEDLEERLSEDLLKLSEDKCLKTTSNIPALPPMNPTPEMFIALIKSSFQTDVFENNIGYLRFDMFGDFEHVATIAQIIVEHVWNKVVDTDALIIDLRNNIGGHASSIAGFCSYFFDADKQIVLDHIYDRPSNTTRDLQTLEQLTGRRYGSKKSVVILTSGVTAGAAEEFVFIMKRLGRAMIIGETTHGGCQPPETFAVGESDIFLSIPISHSTAQGPSWEGAGIAPHIPVPAGAALDTAKGML   
>M4_petMar
ADAPSILRTVGKLVADGYSRAEAALGVPSKLAALLEAGEYGALRSEEELAFKLTVHLQLITGDRHLKAVCVPEHATDRMPGIVPMQMPPTESFEDLIKFSFITDVLEGNIGYLRFDLFSDLEALEHVAHLLVEHVWKKICDTEILIIDLRYNMGGYSTSIPILCSYFFDASPPRHLYTVFDRPSRSSTQVFTVPRVLGQRYGASKDVYILTSHMTGSAGEILTRVMSDLKRATVIGEPTAGGSLSTGTYRIGDSRLYVFIPNQAGVSPSGGRTWSVAGVEPHVQTKASEALQSALRMV   

>Mn_braFlo RALNDQSL SKAIILD LNEL insertions omitted
VDWLDVVMGIGDVMADHYLDQDLLQRWNRTLVHRFQSWSQDDMSDSLRMEEGLTSELRNITGDETIKVWDFGVYENTTQEPVPREFYNFSTFVDNFKKNINVTMLEGNVGYVSIRSMSHIVDIILPDPEMTEFFLSKMAASKAIILDLRYNLGGDREGVVHWASFFFNATPSVPLSDVYYRDGVNQYWTLLEVPGGIRFPDMPLYLLTSNRTSREAEEFAYAMQVVNRTTIIGETTAGEEFTGMWFPIDQTDVHLLTRTNVVRNPITQDSWSGKGVTPDIIVPSEKALTVALRKI
 </pre>

=== RBP3 (IRBP) sequences from human to amphioxus ===
<pre>
>RPB3_homSap human
0 MMREWVLLMSVLLCGLAGPTHLFQPSLVLDMAKVLLDNYCFPENLLGMQEAIQQAIKSHEILSISDPQTLASVLTAGVQSSLNDPRLVISYEPSTPEPPPQV
PALTSLSEEELLAWLQRGLRHEVLEGNVGYLRVDSVPGQEVLSMMGEFLVAHVWGNLMGTSALVLDLRHCTGGQVSGIPYIISYLHPGNTILHVDTIYNRPSNTTTEIWTLPQVLG
ERYGADKDVVVLTSSQTRGVAEDIAHILKQMRRAIVVGERTGGGALDLRKLRIGESDFFFTVPVSRSLGPLGGGSQTWEGSGVLPCVGTPAEQALEKALAILTLRSALPGVVHCLQ
EVLKDYYTLVDRVPTLLQHLASMDFSTVVSEEDLVTKLNAGLQAASEDPRLLVRAIGPTETPSWPAPDAAAEDSPGVAPELPEDEAIRQALVDSVFQVSVLPGNVGYLRFDSFADA
SVLGVLAPYVLRQVWEPLQDTEHLIMDLRHNPGGPSSAVPLLLSYFQGPEAGPVHLFTTYDRRTNITQEHFSHMELPGPRYSTQRGVYLLTSHRTATAAEEFAFLMQSLGWATLVG
EITAGNLLHTRTVPLLDTPEGSLALTVPVLTFIDNHGEAWLGGGVVPDAIVLAEEALDKAQEVLEFHQSLGALVEGTGHLLEAHYARPEVVGQTSALLRAKLAQGAYRTAVDLESL
ASQLTADLQEVSGDHRLLVFHSPGELVVEEAPPPPPAVPSPEELTYLIEALFKTEVLPGQLGYLRFDAMAELETVKAVGPQLVRLVWQQLVDTAALVIDLRYNPGSYSTAIPLLCS
YFFEAEPRQHLYSVFDRATSKVTEVWTLPQVAGQRYGSHKDLYILMSHTSGSAAEAFAHTMQDLQRATVIGEPTAGGALSVGIYQVGSSPLYASMPTQMAMSATTGKAWDLAGVEP
DITVPMSEALSIAQDIVALRAKVPTVLQTAGKLVADNYASAELGAKMATKLSGLQSRYSRVTSEVALAEILGADLQMLSGDPHLKAAHIPENAKDRIPGIVPMQ 0
0 IPSPEVFEELIKFSFHTNVLEDNIGYLRFDMFGDGELLTQVSRLLVEHIWKKIMHTDAMIIDMR 2
1 FNIGGPTSSIPILCSYFFDEGPPVLLDKIYSRPDDSVSELWTHAQVV 1
2 GERYGSKKSMVILTSSVTAGTAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDTNLYLTIPTARSVGASDGSSWEGVGVTPHVVVPAEEALARAKEMLQHNQLRVKRSPGLQDHL* 0
 
>RBP3_bosTau cow run-on terminal exon
0 MVRKWALLLPMLLCGLTGPAHLFQPSLVLEMAQVLLDNYCFPENLMGMQGAIEQAIKSQEILSISDPQTLAHVLTAGVQSSLNDPRLVISYEPSTLEAPP
RAPAVTNLTLEEIIAGLQDGLRHEILEGNVGYLRVDDIPGQEVMSKLRSFLVANVWRKLVNTSALVLDLRHCTGGHVSGIPYVISYLHPGSTVSHVDTVY
DRPSNTTTEIWTLPEALGEKYSADKDVVVLTSSRTGGVAEDIAYILKQMRRAIVVGERTVGGALNLQKLRVGQSDFFLTVPVSRSLGPLGEGSQTWEGSG
VLPCVGTPAEQALEKALAVLMLRRALPGVIQRLQEALREYYTLVDRVPALLSHLAAMDLSSVVSEDDLVTKLNAGLQAVSEDPRLQVQVVRPKEASSGPE
EEAEEPPEAVPEVPEDEAVRRALVDSVFQVSVLPGNVGYLRFDSFADASVLEVLGPYILHQVWEPLQDTEHLIMDLRQNPGGPSSAVPLLLSYFQSPDAS
PVRLFSTYDRRTNITREHFSQTELLGRPYGTQRGVYLLTSHRTATAAEELAFLMQSLGWATLVGEITAGSLLHTHTVSLLETPEGGLALTVPVLTFIDNH
GECWLGGGVVPDAIVLAEEALDRAQEVLEFHRSLGELVEGTGRLLEAHYARPEVVGQMGALLRAKLAQGAYRTAVDLESLASQLTADLQEMSGDHRLLVF
HSPGEMVAEEAPPPPPVVPSPEELSYLIEALFKTEVLPGQLGYLRFDAMAELETVKAVGPQLVQLVWQKLVDTAALVVDLRYNPGSYSTAVPLLCSYFFE
AEPRRHLYSVFDRATSRVTEVWTLPHVTGQRYGSHKDLYVLVSHTSGSAAEAFAHTMQDLQRATIIGEPTAGGALSVGIYQVGSSALYASMPTQMAMSAS
TGEAWDLAGVEPDITVPMSVALSTARDIVTLRAKVPTVLQTAGKLVADNYASPELGVKMAAELSGLQSRYARVTSEAALAELLQADLQVLSGDPHLKTAH
IPEDAKDRIPGIVPMQ 0
0 IPSPEVFEDLIKFSFHTNVLEGNVGYLRFDMFGDCELLTQVSELLVEHVWKKIVHTDALIVDMR 2
1 FNIGGPTSSISALCSYFFDEGPPILLDKIYNRPNDSVSELWTLSQLE 1
2 GERYGSKKSMVILTSTLTAGAAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDTDLYLTIPTARSVGAADGSSWEGVGVVPDVAVPAEAALTRAQEMLQHTPLRARRSPRLHGRRKGHHRQSQGRAGSLGRNQGVgRPEVLTEAPSGQKRGLLQCG* 0

>RBP3_monDom opossum
0 MTSQCLLLFSALLFSLAHAEQIFQPSLVRDMAKILLDNYCFPENLMGMQEVIEQAIKSGEILDISDPQMLASVLTAGVQGALNDPRLVISFEPSIPETPQ
HVPKLANVTQEELLILLQQMIKYQVLEGNVGYLRVDYIPGQEVVEKVGEFLVNNIWKKLMGTSSLVLDLQHSSGGEISGIPFVISYLHQGDILLHVDTVY
DRPSNTTTEIWTLPQVLGERYGGEKDMVVLTSHRTVGVAEDIAYILKKLRRAIVVGEQTLGGALDLRKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSG
VLPCVGIPAEQALGKALAILTLRRARPGAIQRLMEVLQNYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPEEATMGSEASEEEDATPAANS
LPEDESQRQALVDSVFQVSVLPGNVGYLRFDEFADSSVLGTLAPYVIRQVWEPLQDTNHLIMDLRYNPGGPSSAVPLLLSYFQDPAAGP
IRLFTTYDRQTNQTQEHLSRAELLGKPYGAQRGVYLLTSHHTATAAEEFAFLMQSLGRATLVGEITAGSLMHTRTFPLLQPPNGNLVLTVPILTFIDNNG
ECWLGGGVVPDAIVLAEEALDKAKEVLEFHQRLGALVEGTGHLLEAHYALPEVVGQASALLKAKLEHGTYRTAVDFESLASQLTSDLQEVSGDHRLHVFH
SPGEPVSEELTPPQKGVPSPEELTYLIEALFKTEVLPGQLGYLRFDMMAEAETVRAIAPQLVELVWEKLVHTEALVVDLRYNPGGYSTAVPLLCSYFFEA
EPRRHLYTIFDRAASQLTEVWTLPQVAGERYGSQKDLYILISHTSGSAAEAFVHTMKDQHRATVIGEPTGGGALSVGIYQVENSPLYASMPTQVAISPVT
GKAWDMAGVEPDVSVLSSEALMTTQGIVALRAKVPTILQTAGKLVADNYASLEVGSRVASKLAKLQTQYRQVTSEGELADMLGADLQTLSGDRHLKTAHI
PEDAKDRIPGIVPMQ 0
0 LPSPEAFEDLIKFSFHTNVFEGNIGYLRFDMFGDCELLTQVSDLLVEHVWKKVVHTDGMIIDMR 2
1 FNIGGPTSSISALCSYFFDEGQEVLLDQIYNRPNDSISEIWTQSQVA 1
2 GERYGSKKSVIILTSSMTAGAAEEFVYVMQRLGRALVIGEVTSGGCQPPQTYHVDDTDLYITIPTARSVGSGDKPSWEGVGVAPHVEVPADQALSKAKEMFNHHLQRAK* 0

>RBP3_sacHar Sarcophilus harrisii
0 MTSQCLLLFSALLFSLAHGEEIFQPTLVLDMAKILLDNYCFPENLMGMQEAIEQAIKSGEILDISDPQMLANVLTAGVQGSLNDPRLVISYEPSTSEAPQ
HDPKFANATQEELLALFQKIIKYQVLEDNVGYLRVDYIPGRDMIEEVGEFLVNDIWKKVMETSSLVLDLQHSR
GGEVSGIPFVISYLHQGDILLHVDTIYDRPSNTTTEIWTLPQVLGERYSGEKDIVVLTSHHTVGVAEDIAYILKKMRRAIVVGEQTQGGSLDLRKLRIGQSDFFITVPVARSLSPLGGGSQTWEGSG
VVPCVGIPAEQALEKALAILTLRRARPGAIQRLMEILQKYYTLVDRVPALLHHLTAIDYSSVLTEEDLAAKLNAMLQAVSEDPRLLVRVLRPEEATVEAEPGEESATPASVSLPESDAERQALI
DSVFQVSVLPGNVGYLRFDEFADNSVLGTLAPYVLRQVWEPLQDTDHLIMDLRYNPGGPSSAVPLLLSYFQDPSAGPVRLFATYDRQTNQ
TQEYRSRAELLGKPYGAERGVYLLTSYHTATAAEEFAFLMQSLGRATLVGEITAGSLMHTRTFPLLQPPNGSLVLTVPTLTFIDNHGECWLGGGVVPDAIVLAEEALDKAKEVLEFHQRL
GALVEGTGHLVEAHYALPEVVGQASAFLRATLAHGTYRTAVDFESLASQLTSDLQEVSGDHRLHVFHSPGEPVPEESSPPHKGVPSPEELTYLIEALFKTDVLP
QLGYLRFDMMAEVETVRAIGPQLVELVWEKLVNTEALVVDLRYNPGSYSTTVPLLCSYFFEAEPRKHLYTVFDRATSQFTEVWTLPQVTGERYGSQKDLYILISHTSGSA
AEAFAHIMKDLQRATVIGEPTAGGALSVGIYQVGDSPLYVSMPTQVALSPVTGKAWDMAGVEPDVSVLANEALITAQGIVALRAKVPTILQTAGKLVADNYA
SPEVGSRVAAKLASLQIQYGKVTSEGELADMLGADLQTLSGDRHLKTAHIPEDAKDRIPGIVPMQ 0
0 LPSPEAFEDLIKFSFHTNVFEGNIGYLRFDMFGDCELLIQVSDLLVEHVWKKVMHTDGMIIDMR 2
1 FNIGGPTSSISAMCSYFFDEGQGVLLDRIYNRPNDSISEIWTQPQVI 1
2 GERYGSKKTVVILTSSMTAGAAEEFVYIMQRLGRALVIGEVTSGGCQPPQTYHVDDTDLYITIPTTRSVVSGDKSSWEGVGVVPHMEVPADQALSKAKEMFTHQLQKTK* 0

>RBP3_macEug wallaby 3 alleles to FJ603206 Macropus eugenii frag exon 2 lower case: sacHar fix
0 MTSQCLLLFSALLLSLAHAEQIFQPSLVLDMAKILLDNYCFPENLMGMQEAIEQAIKSGEILDISDPQMLASVLTAGVQGSLNDPRLVISYEPSPAEAPQQSPKLTSLTQEELLTLLQQM
IKYQVLDGNVGYLRVDYIPGQEVVEKVGEFLVNDIWKKLMGTSSLVLDLQHSTEGEVSGIPFVISYLHEGDILLHVDTVYDRPSNTTTEIWTLPQVLGERYSGEKDLVILTSHRTVGVAE
DIAYILKKMRRAIVVGEQTLGGALDLRKLRIGQSDFFITVPVSRSLSPLGGGSQTWEGSGVLPCVGIPAEQALEKALAILTLRRARPAAIQRLMEVLQNYYTLVDRVPALLHHLTAIDYS
SVLTEEDLAAKLNAGLQAVSEDPRLLVRVLRPEEATAGAESREEAATAAPVPLPDGESQRQALVNSVFQVSVLPGNVGYLRFDEFADSSVLGALAPYVLQQVWEPLQDTDHLIMDLRYNP
GGPSSAVPLLLSYFQDPAAGPVRLFATYDRQTNQTREYRSQAELLGKPYGAQRGVYLLTSHHTATAAEEFAFLMQSLGRATLVGEITAGNLMHTRTFSLLQPPDGSLVLTVPILTFIDNH
GECWLGGGVVPDAIVLAEEALDKAKEVIEFHQRLGALVEDAGHLLEAHYALPEVVGQASALLRARLVHGTYRTAVDFESLASQLTSDLQEVSGDHRVHVFHSPGELIPEELSPPQNVVPS
PEELTYLIEALFKTEVLPGQLGYLRFDMMAEAETVRAIGPQLIELVWEKLVNTEALVVDLRYNPGSYSTSVPLLCSYFFEAEPRKHLYTIFDRAASQATEVWTLPQVAGERYGSQKDLYI
LISHTSGSAAEAFAHAMKDLRRATVIGEPTAGGALSVGIYQVSNSPLYASMPTQVAISPVTGKAWDIAGVEPDVSVPAREALITTQGIVTLRSKVPTILQTAGKLVADNYASPEVGSRVA
AKLARLQTQYRQVTSEGELADMLGADLQTLSGDSHLKTAHIPEDSKDRIPGIVPMQ 0
0 lpspeafedlikfsFHTNVFEGNIGYLRFDMFGDCELLTQVSDLLVEHVWKKVVHTDGMIIDMR 2
1 FNIGGPTSSISALCSYFFDEGQKVLLDRIYNRPNDSIVEIWTQPHVT 1
2 GERYGSKKSVIILTSSTTAGAAEEFVYIMQGLGRALVIGEVTSGGCQPPQTYHVDDTDLYITIPTAQSVGSGDRPSWEGIGVTPHVEVPADQALSKAKEMFIHHLQRAD* 0

>RBP3_ornAna platypus genome rife with frameshifts, dels, misassembly frag
0 MGVCLPLLLVAQFSLTGHVEPVSQPSMVLDVAKILLDNYCYPENLMGMQEAIEEAIQRGEILDIADPKRLASVLTAGVQGSLNDPRLVISYEPAPVAVSQ
QPPEPASLPAEQPLERLRPAVGSEVLEGNVGYLRVDRLPGREEIERVGAVLGRDIWEKLLGTSALVLDLRHSTGGHVSGIPFFISYFYPEGPALHVDTVY
DRPSNATRQLWTLPRVLGARYAADKDVVVLTSRLTAGVAEDVAYILQQMRRAIVVGERTAGGPLVFRKLRVGLSDFFITVPVACSLGPLGGGGRSWEGSG
VLPCVAVPADRALDEALDILALRGAVPGAVAHLADLLRDYYALVDRVPALLRHLAALDLSSVLSEEDLTSRLNAGLQAASEDPRLLVRRLEPEEAERGPP
RKEEEQKEEEEEDQPSPGASILPGDGSSLFRVSVLPGNVGYLCFDEFPEASALERLGPLLGRRVWEPLEATDHLMVDLRNNPGGPSSAVPLLLSYF
QDPAAGPIRLFTTYNRPADVTREYASRAGALEKPYGARRGVYLLTSHRTATAAEEFAYLMQALGRATLVGEITAGRLLHSRTFPLLRPPWEGLVLTVPFL
TLFDPHGEGWLGGGVVPDAIVLAEEALEKAGEVLAFHQTLEALVETTGHLLEAHYCFPAGARRAGAQPWPVAGVEPDVMAQAAEALAVAQGIAALRSKVP
TVLRTAAKLVADNYAFRETGAGVAAQMGGLQARCGRVTSEGALAEVLGAHLRALSGDPHLQMVYIPEDAKDRIPGVVPMQ 0 
0 IPSAETFEDLIKFSFHTSVMEGNIGYLRFDMFGDCELLTQVSELMVEHVWKKIVHTDGLIIDMR 2
1 NIGGPTSSISALCSYFFDEDHPVLLDKIYNRPNDSISEIWTHSHIA 1
2 GERYGSRKSVVILTSNMTAGAAEEFVSIMKRLGRALVVGEVTGGGCHPPQTYHVDDTHLYITIPTSRSVGSEDGSSWEGVGVTPHLVVPADVALSRAKDLFRAHLEHRD* 0

>RBP3_taeGut Taeniopygia guttata
0 MIRTHFLLLSALIMCSIPAEEIFQPTLVLDMAKVLLDNYCYPENLVGMQEAIEQAIKSGEILDISDPKMLANVLTAGVQGALNDPRLVISYEPLPHSGPK
QEAEGSPTREQLLSLIEHVIMYDKLEGNVGYLRIDYIIGEEVVQKVGAFLVDKVWKTLIETSALVIDLRHSTGGQISGLPFIISYLHEQDKILHVETVYN
RPSNTTTEIWTLPKVLGERYSKDKDVIVLISHHTTGVAEDVAYILKHMNRAITVGEKTAGGSLDIQKLRIGPSNFYMMVPVSRSVSPLSGGGQSWEVSGV
MPCVATEAEQALQKSLDILAVRRAVPGTISHLKNILKDYYSLVERVPALLRRLTTSDFSSVQSSEDLATKLNTELQALSDDPRLMVRVMMPGEAADSPAE
KPVGMAADLPDNEQLLHALVDTVFKVSVLPGNVGYMRFDEFADASVLVKLGPYLVHKVWEPLQNTENLIMDLRYNLGGPSSSAVPVLLSYFQDPAAGPVH
LFTTYDRRTNHTQEHNSQAELLGQSYGAKRGVYLLTSHHTATAAEEFAYLMQSLGRATLIGEITAGSLSHTRTFPLLQPGPGITRGLTITVPVITFIDNH
GESWMGGGVVPDAIVLAEDALEKAEEVLAFHKNMGVLLEGTGQLLEDHYAIPEVAAKASAMLSTKRAQGGYRSAIDSETLASQLTSDLQEASGDHRLHVF
HSHVEPTPEEQLPNVIPSPEELSYIIEALFKIEVLPGNLGYLRFDMMAEAETVKAIGPQLLQMVWNKLVDTDAMIIDMRYNTGGYSTAIPILCSYFFDPE
PRKHLYTVFDRSTSRSTEVWTLPQLAGKRYGSLKDIYILTSHMSGSAAEAFTRSMKDLHRATVVGEPTVGGSLSVGIYRVGNSSLYASIPSQVVLSPVTG
KVWSVSGVEPHITIQASEAMAAAQHIANLRAQVPQILQTVGKLVADNYAFVNTGTVIASNLTKNIHKDNYKRINTEEDLAGKVTAILQALSDDKHLKLLY
IPEHAKDSIPGIMPK 0
0 QIPPPEVFEDLIKFSFHTNVFENNIGYLRFDMFGDSELLTQLSDLMIEHVWKKIFHTDALIIDLR 2
1 YNIGGSTTPIAILCSYFFDEGHPVLLDRVYDRPSDSVKEIWTQPQLK 1
2 GERYGSQKGLVILTSAVTAGAAEEFVYIMKRLSRALIIGEQTSGGCHSPQTYQVDETNFYVVIPTSRSVTSADSTSWEGKGVSPHIETPAETALIKAKEMLNAHLHSSR* 0

>RBP3_galGal Gallus gallus 1236 aa N-terminal 21 aa signal peptide 5 glyc (3 unique) two W per repeat
0 MRTYFFLFSVLIVCSISAEEIFQPTLVLDMAKVLLDNYCYPENLVGMQEAIEQAIKSGEILDISDPKMLANVLTAGVQGALNDPRLVISYEPSLHAAPKQ
EAETYPTREQLLSLIEHVVIYDKLEGNVGYLRIDYIIGQEVVEKVGAFLVDKVWKTLINTSALVIDLRYSTGGQISGIPFIISYLHEADKMLHVETVYNR
PSNTTTEIWTLPKVLGERYSKDKDVIVLISHHTTGVAEDVAYILKHMNRAITLGEKTAGGSLDIQKLRIGPSNFYMMVPVSRSVSPLSGGGQSWEVSGVM
PCVASEAEQALKKSLDILAVRRAVPGTLSRLTDILKDYYSLVERVPVLLRHLTTSDFSSVQSAEDLATKLNTEMQTLSEDPRLLVRTMMPGEAAAPPAEM
PIAMAANLPDNEQLLHALVDTVFKVSVLPGNVGYMRFDEFADASVLVKLGPYIVKKVWEPLQNTENLIMDLRYNPGGPSSSAVPMLISYFQDPTAGPVHL
FTTYDRRTNHTQEHNSQAELLAQPYGAQRGIYVLTSRHTATAAEEFAYLMQSLGRATLIGEITAGSLSHTCTFPLVQPEQGITRGLTITVPVITFIDNHG
ESWMGGGVVPDAIVLAEDALEKAEEVLTFHRKMGILLESTGQLLEAHYAIPEVAEKASVMLSTKRVQGGYRSAVDFETLASQLTSDLQEASGDHRLHVFH
SHVEPTPEEQLPNMIPSPEELSYIIEALFKIEVLPGNLGYLRFDMMAEAETVKAIGPQLVQMVWNKLVDTDAMIIDMRYNTGGYSTAVPILCSYFFEPEP
RQHLYTVFDRSTSRSTEVWTLPKVTGKRYGSLKDIYILTSHMSGSAAEAFTRSMKDLHRATVIGEPTVGGSLSVGIYRVGNSSLYRSIPSQVVLSPVTGK
VWSVSGAEPHITIQASEALAAAKHIASLRTQVPQIVQTVGKLVAENYAFVDIGTDIASNLTKSVNKENYKRINSEKELARKLTAILQALSDDEHLKILYI
PEHAKDSIPGILPK 0
0 QIPSPEVFEDLIKFSFHTNVFENNIGYLRFDMFGDCELLTQVSDLLVEHVWKKIVHTDALIIDMR 2
1 YNIGGYTNSIPILCSYFFDEGHQVLLDKVYDRPSDSVKEIWTQPQLR 1
2 GERYGSQKGLIILTSAVTAGAAEEFVFIMKRLGRALIIGEQTSGGSHSPQTYQVDDTNFYIIIPTARSVISAESASWEGKGVPPHMETPAVTALIKAKEVLSAHLHSSR* 0

>RBP3_anoCar lizard
0 MLRKCLWLSIVLVCCSSYADSVLQSTLVLDMAKLLLDNYCLPENLVGMREAIEQAIKNGEVLDISDPKLLATVLTAGVQGALNDPRLVISYEPTAPAAPK
QRMETSLTPEQLLSLIQHTVKYEVLDDNVGYLRIDYIMGQDIVQKIGSFLVEKVWKTLLGTSALILDLRYTTGGDVSGIPFIISYLYNGDKVLHVDTVYN
RPSNTTVEILTLPKVLGVRYSKDKDVILLISKYTTGVAENVAYILKHMHRTIIVGEKSAGGSLDTQKMQIGNSQFYMTVPLSCSVSPLSGSGQSWEISGV
TPCVVISAEQALDKALAILSLRKAIPNSMSYLVDIIKNNYSMLEQVPVLLQHLSTFDYSSVLSVKDLASKLNAELQTISEDPRLFLRVPASDEAVTSQTD
EKVAMASDLPNNEQLMKALVMTVFKVSVLPGNVGYMRFDEFGDATVLVKLGPYLLQHVWEPLQATDYLIIDLRYNIGGPSSSAVPVLLSYFQDPSAGPVH
FFTTYNRLTNQTQAYSSSAEMVGKPYGARRGVYLLTSHNTATAAEEFAYLMQTLGRATLVGEITAGSLSHTHTFCILELGGGCGLLINVPVITLIDNHGE
YWLGGGVVPDSIVLADEALEKAREVLEFHKGMGSLIERVGQLLEAHYAIPEMARRVSSMLNSKLAQGGYRTAVDFETLASQLTNDLQETSGDHQLHVFHS
HVEPSLEEQSPFKTLTPEELNFIIEALFKVDVLPGNVGYLRFDMMAEFESVKTIEPQILHMVWEKLVETSAMIVDMRYNTGSYSTAVPMFCSYFFDAEPQ
QHLYTIIDRSTSQSTEVWTSSQVSGKRYGSTKDLYILISHASGSAAEAFTRSLKDLHRATVIGEPTVGGSLSASIYNIGSTPLYASIPSQIVLSPVSGKV
WSLSGIQPHVTTQSNEALASAQNIILFRTKLPSVLNTIGKLVADNYAFADIGATVAAKFADYAKKGTYRKINSEIELSGKLAADLKALSGDRHLMISHIP
ERSKGRILGLVPM 0
0 QIPPPEILEDLIKFSLHTNVFENNIGYLRFDMFGDCELMSQVSELLVQHVWNKIVNTDALIIDMR 2
1 YNVGGPACSVPLLCSYFFDEGHPILLDKVYNRPNDTTSNIWTVSKLA 1
2 GKRYGLNKGLIILTSSVTSGAAEEFAHIMKRLGRAFIIGQKTSGGCHPPQTFHVDGTNLYITTPVSRSVFSVNDSWEGVGVSPHLDVSTDVALIKAKEMLKAHLH* 0

>RBP3_xenLae Xenopus laevis
0 MPPLFQALTTALFFCGIASNPLFQPSLVMDMAKVLLDNYCFPENLVGMQETIEQAVKGGEILHISDPDTLANVFTSGVQGYLNDPRLVVSYEPNYSGPQT
EQSLELTPEQLKFLINHSVKYDILPGNIGYLRIDFIIGQDVVQKVGPHLVNNIWKKLMPTSALILDLRYSTQGEVSGIPFVVSYLCDSEIHIDSIYNRPS
NTTTDLWTLPELMGERYGKVKDVVVLTSKYTKGVAEDASYILKHMNRAIVVGEKTAGGSLDTQKIKIGQSDFYITVPVSRSLSPLTGQSWEVSGVSPCVV
VNAKDALDKAQAILAVRSSVTHVLHQLCDILANNYAFSERIPTLLQHLPNLDYSTVISEEDIAAKLNYELQSLTEDPRLVLKSKTDTLVMPGDSIQAENI
PEDEAMLQALVNTVFKVSILPGNIGYLRFDQFADVSVIAKLAPFIVNTVWEPITITENLIIDLRYNVGGSSTAVPLLLSYFLDPETKIHLFTLHNRQQNS
TDEVYSHPKVLGKPYGSKKGVYVLTSHQTATAAEEFAYLMQSLSRATIIGEITSGNLMHSKVFPFDGTQLSVTVPIINFIDSNGDYWLGGGVVPDAIVLA
DEALDKAKEIIAFHPSIFPLVKGTGHLLEVHYAIPEVAYKVSSVLQNKWSEGGYRSVVDLESLASLLTSEMQENSGDHRLHVFYSDTEPEILEDQPPKIP
SPEELNYIIDALFKIEVLPGNVGYLRFDMMADTEIIKAIGPQLVSLVWNKLVETNSLIIDMRYNTGGYSTAIPIFCSYFFDPEPLQHLYTVYDRSTSTGK
DIWTLPEVFGERYGSTKDIYILTSHMTGSAAEVFTRSLKDLNRATLIGEPTSGVSLSVGMYKVGDSNLYVTIPNQVVISSVTGKVWSVSGVEPHVIIQAN
EAMNIAHRIIKLRTKIPTVIQTAAKLVADNYAFADTGANVASKFIALVDKIDYKMIKSEVELAEKINDDLQSLSKDFHLKAVYIPENSKDRIPGVVPM 0
0 QIPSPELFEELIKFSFHTDVFEKNIGYIRFDMFADSDLLNQVSDLLVEHVWKKVVDQDALIIDMR 2
1 FNIGGPTSSIPIFCSYFFDEGTPVLLDKIYSRTSNAMTDIWTLPDLV 1
2 GKTFGSKKPLIILTSSLTEGAAEEFVYIMKRLGRAYVVGEVTSGGCHPPQTYHVDDTHLYLTIPTSRSASAEPGESWEGKGVLPDLEISSETALLKAKEILESQLEGRR* 0

>RBP3_xenTro Xenopus tropicalis 89% xenLae
0 MSPLFKALTTVLFFCIVASNPVFQPSLVMDMAKVLLDNYCFPENLVGMQETIEQAMKSGEILHISDPETLANVFTSGVQGFLNDPRLVVSYEPNYSGPRK
EQSPEPTLEQLKFLLDHSVTYDLLPGNIGYLRIDFIIGQDVVQKVGPLLVNNIWKKLMPSSALILDLRYSTQGKVSGIPFVVSYLTDPQIHIDSIYNRPS
NTTTDLWTLSELMGERYGKDKDVVVLTSKYTEGIAEGAAYILKHMSRAIVVGEKTAGGSLDIQKIKIGQSEFYITVPVSRSISPLTGQSWEVAGVFPCVV
VNANNALNKAQGILAVRSSITHILLQLSEILVNNYAFSERIPTLLQHLPNLDYSSVISEEDITAKLNYELQSLTEDPRLVLKSKTDSLVMPEDSTQVENL
PDDEATLQALVNTVFKVSILPGNIGYLRFDEFADVSVLAKLGPYIVNTVWDPITVTENLIIDLRYNIGGSSTSIPLLLSYFQEPENRIHLFTIYNRQQNS
TNEVYSLPKVLGKPYGSKKGVYVLTSHETATAAEEFAYLMQSLSRATIIGEITSGNLMHSKAFPLDGTRLSVTVPIMNFIDNNGDYWLGGGVVPDAIVLA
DEALDKAKEIIAFHPSVFALVEGTGHLLEVHYAIPEVAYKVSSVLQNKWSEGGYRSVVDLESLASQLTSEMQENSGDHRLHVFYSDTEPEILEDQPPKIP
SAEELNYIIDALFKIEVLQGNVGYLRFDMMADTEIIKAIGPQLVSLVWNKLVETNSLIIDMRYNTGGYSTAIPIFCSYFFDPEPLQHLYTVYDRSTSSGT
DIWTLPEVVGERYGSTKDIYILTSHMTGSAAEVFTRSMKELNRATIIGEPTSGVSLSVGMYKVGESNLYVSIPNQVVISSVTGKVWSVSGVEPHVIAQAS
EAMNVAHHIIKLRTKIPSVIQTAGKLVADNYAFADTGADVASKLIALVDKINYKMIKSEVELAEKLNYDLQSLSKDVHLKAVYIPENSKDRIPGVVPMQ 0
0 IPSPEMFEDLIKFSFHTDVFEKNLGYIRFDMFADSDLLNQVSDLLVEHVWKKVVNQDALIIDMr 2
1 FNIGGPTSSIPTFCSYFFDEGTPVLLDKIYSRTTNAITDVWTLPHLV 1
2 GNAFGSKKPVIILTSSLTEGAAEEFVYIMKRLGRAYVIGEVTSGGCHPPQTYHVDDTHLYLTIPTSRSASAKPGESWEGKGVLPDLEITSETALMKAKEILVSQLEGR* 0

>RBP3_tetNig frameshifts in genome two domains: 23-324,326-612 no upstream dup
0 MAKALFTVASLLLLANGFFVGAAFPPSLIADMAKIVLDNYCSPEKLAGMKEAIKAAGTNTEVLNIPDGESLARVLSAGVQGTVSDPRLMVSFQPNYVPAG
PHKMPPLPPEHLVAVLQTSVKLDILEGNTGYLRIDHILGEEVADKVGPALIDLIWNKILPTSALIFDLRYTSSGDISGIPYIVSYFTQAEPVVHIDSVYD
RPSNTTTKLLSLPNLLGQRYGVSKPLIVLTSKNTKGIAEDVAYCLKNLKRATIVGEKTAGGSLKLDTFKVGDTDFYITVPTAKSINPITGSSWEIRGVTP
HVEVNAEDALATAIKIVNLRAQIPAIIEGTAALVANNYAFEATGADVAKELRELQANGQYSSVVSKESLEAALSADLQRLSGDKSLKTTPNTPVLPPM 0
0 DYTPEMYIELIKVSFHTDVFENNIGYLRFDMFGDFEEVKAIAQIIVEHVWNKVVNTDALILDLr 2
1 NNVGGPTTAIAGFCSYFFDADKQNRVGQAVRQASGTTTELLTLSELT 1
2 GVRYGSKKSLIILTSGATAGAAEEFVYIMKKLGRAMIVGETTAGASHPPQTFRVGETDVFLLIPTVHSDTGAGPAWEGAGIAPHIPASAEAALGTARAILNKHFAGQK* 0

>RBP3_takRub fugu two domains:  23-324,326-612 plus upstream dup
0 MAKALFLVASLLLLANDVLVRAAFPPSLITDMAKIVLDNYCSPEKLAGMKEAIEAAGTNTEVLNIPDGESLARVLSAGVQGTVSDSRLMVSYQPDYVPAV
PPKMPPLPPEHLVAVLQTSIKLDLLEGNTGYLRIDHIIGEDVAEKVGPSLIDLIWNKILPTSALIFDLRYTSSGEISGIPYIVSYFTQAEPVVHIDSVYD
RPSNTTTKLFSLSNLLGERYGITKPLIILTSKNTKGIAEDVAYCLKNLKRATIVGERTAGGSVKLDNFKVGSTDFYITVPTAKSINPVTGSSWEITGVKP
DVEVNAEDALATAIKIVSLRAQIPAIIEGAATLIAKNYAFEATGADVATKLRELLAKGQYNSVVSSESLEVALSADLQRLSGDKSLKATQNAPVLPPM 0
0 DYSPEMYIELIKVSFHTDVFENNIGYLRFDMFGDFEEVKAIAQIIVEHVWNKVVNTDALILDLR 2
1 NNVGGPTTAIAGFCSYFFDADKLIVLDKLHDRPSGTTTELLTLPELT 1
2 GVRYGSKKSLIILTSGATAGAAEEFVYIMKKLGRAMIVGETTAGASHPPQVFSVGEIGIFLSIPTVHSDTAAGPAWEGTGITPHIPVSAEAALGTAKGILNKHFGGQK* 0

>RBP3_gasAcu sticklebck two domains: 27-317,323-612 no upstream dup
0 MAKLIFLVAPLLVLGNIAFIHAGFAPNVIIDMAKIVIDNYCSPEKLAGMKEAIEAAGSNTEVLSIPDAETLANVLSAGVQTTVSDPRLMISYEPNYVPVV
PPKMPPLPPDQVIAVLQTSIKLDILEGNIGYLRIDHILGEDVAEKVGPLLLDLVWNKILPTSALIFDLRYTSSGDISGIPYIVSYFTEAGTPIHIDSIYD
RPSNTTTKLFSMSTLLGERYSTSKPLIILTSKNTKGIAEDVAYCLQNLKRATIVGEKTAGGSVKVDKIQVRDTGFYVTVPTAKSVNPITGSTWEVTGVTP
NVEVNAEDALATAIKIVTLLNRVPAIIEGSATLIADNYAFEDIGAAVAEKLKGLLANGEYSKVVSKDSLEMKLSADLRTLSGDKSLKTTSNVPALPPM 0
0 NYSPEMYIELIKVSFHTDVFEDNIGYLRFDMFGDFEEVKAIAQIIVEHVWNKVVNTDAMIVDLR 2
1 NNIGGPTTAIAGFCSYFFDSDKQIVLDRLYDRPSGTTTELRTLPELT 1
2 GTRYGSKKSLVMLTSRATAGAAEEFVYIMKKLGRAMIVGETTAGTSHPPKTFRVGETDIFLSIPTVHSDTAAGPAWEGAGVAPHIPVPADAALETAKGIFKKHFAGQK* 0
 
>RBP3_oryLat medaka two domains: 28-314,320-605  no upstream dup
0 MAKTLFLVASLLVLGNVVFLHASFPPSLITDLAKIVMDNYCSPEKLSGMKEDIATAGANTDVLNIPDGEALAKVLTDGVQTTVSDPRLRVSYEPNYVPVV
PPQLPPEQLIAVLQTSIKLDILEGNIGYLRIDSIIGEEVAEKVGPLLLELVWSKILPTSALIFDLRYTSSGDITGIPYIISYLTDAKSEIHIDTIYDRPL
NTTTKLLSMQSTLGQTYGGTKPLLVLTSKNTKDIAEDVAYCLKNLKRATIVGEKTAGGSAKIKKFRVGDTDFYVTLPTAKSINPITGSSWEVTGVKPNVE
VNAEEALATALKIINLRLQVPAIIEESATLVANNYAFESTAADVAEKLKGHLANGDYNMVVSKESLEAKLSADLQSLSGDKSLTVSSNTGAPPPM 0
0 EYTPEMYIELIKISFHTDVFENNIGYLRFDMFGDFEEVKAIAQVIVEHVWNKVLHTDAMIIDLR 2
1 NNVGGPTTAIAGFCSYFFDGDKQILLDKLYDRSTGTTTDLLTLGELT 1
2 GERYGSKKSLIILASRATAGAAEEFVYIMKRLGRAMIVGETTAGASHPPKVFQVGESDIFLSIPTVHSDTSAGPGWEGAGVAPHIPVAAGAALETAKAILNKHIGGQQHAAS* 0

>RBP3_danRer zebrafish upstream frag as well two domains: 22-322,324-609
0 MAQALVLLVSLLFFSNVAHCNFSPTLIADMAKIFMDNYCSPEKLTGMEEAIDAASSNTEILSISDPTMLANVLTDGVKKTISDSRVKVTYEPDLILAAPP
AMPDIPLEHLAAMIKGTVKVEILEGNIGYLKIQHIIGEEMAQKVGPLLLEYIWDKILPTSAMILDFRSTVTGELSGIPYIVSYFTDPEPLIHIDSVYDRT
ADLTIELWSMPTLLGKRYGTSKPLIILTSKDTLGIAEDVAYCLKNLKRATIVGENTAGGTVKMSKMKVGDTDFYVTVPVAKSINPITGKSWEINGVAPDV
DVAAEDALDAAIAIIKLRAEIPALAQAAATLIADNYAFPSIGEHVAEKLEAVVAGGEYNLISTKEDLEERLSEDLLKLSEDKCLKTTSNIPALPPM 0
0 NPTPEMFIALIKSSFQTDVFENNIGYLRFDMFGDFEHVATIAQIIVEHVWNKVVDTDALIIDLr 2
1 NNIGGHASSIAGFCSYFFDADKQIVLDHIYDRPSNTTRDLQTLEQLT 1
2 GRRYGSKKSVVILTSGVTAGAAEEFVFIMKRLGRAMIIGETTHGGCQPPETFAVGESDIFLSIPISHSTAQGPSWEGAGIAPHIPVPAGAALDTAKGMLNKHFSGQK* 0

>RBP3x_takRub fugu single upstream exon 42% frameshift no transcripts three domains: 23-323,325-615,618-907
MAPRTPVLLLVLLFCALPVRSFYQHTLVLEMAKLLLENYCIPENLVGMQEAIQRAIKSREILQISDRKTLATVLTVGVQGALNDPRLSVSYEPSFSPLPLQALSSLPVEQQLRLLRN
SIKLDILDSDVGYLRIDRIIDEETLLKFGPLLRENVWDKAAQTSSLILDLRFSTAGGWSGIPSIVSYFTEPHSLVHIDTVYDRPSNTTTELWTMSSVRGK
TFGGKKDMIVLIGRRTAGAAEAVAYTLKHLNRAIVVGERSAGGSLKVRKFRIAESDFYITMPVARSVSPITGKSWEVSGISPTVNVAAREALAKAQTFLA
VRSRIPKVLQIVLDIIGRFYAFADRVQALLQQLESADLFSVVSEEDLAARLNHDLQTASEDPRLIIRHKRDNIPRAEEEPELHAANDHDGELVEGFTVQV
LPHNTGYLRLDRFVRCSEGDKLEEIVAEKVWGPLKDTQNLIIDLRHNTGGSSTSVALLLSYLRDPLPKRHFFTIYDSVQNTTTEYGSRPHIPGPSYGSER
GVYVLTSHYTAGAAEEFAYLIQSLHFGTVVGEITSGTLMHSKTFQVEGTDIFITVPFINFLDNNGEYWLGGGVVPDAIVLAEEALEHVNRTATFHQGLRSLIGRTGELLEKHYAIQEVAQKVGEV
LLSKWAEGLYRSVVDLESLASQLTADLQEASGDHRLHVFRCDVELESLHGVPKIAAVEEAGFVIDALFKSELLPRNVGYLRFDTMADIEAAKGAAPRLVKSVWNKLVDTDSLIIDMRYNA
GGSSTAVPLWCSYFVDGEPLQHLYTVYDRTTKTRVEVMTLPEVSGQRYDPGKDVYILTSHMTGSAAEAFVRAMRDLNRVTIVGEPTAGGSLSSATYQIGESVLYASIPNQVVTSAATGKL
WSISGVEPDVFAQARDALPVAQRIISARLLKREKGR* 0

>RBP3x_danRer zebrafish single upstream exon 55%/41% transcript DN857398 3 domains: 21-321,324-609,612-901 expressed: inner nuclear layer and ganglion cell layer
MAGVFVFILVTYRVLLVNASFQSALVLDMAKILLDNYCFPENLIGMQEAIQQAINSGEILHISDRKTLASVLTAGVQGALNDPRLTVSYEPNYTLITPPA
LHSLPTEQLIRLIRSTVKLEVMDNNIGYLRIDRIIGQETVVKLGRLLHNNIWKKVAHTSAMIFDLRFSTAGELSGLPYIVSYFSDSDPLLHIDTIYERPT
NITRELWTLPTLLGERFGKRKDLIVLISKRTIGAAEGVAYILKHLKRAVIIGERSAGGSVRVDKLKIGDSGFYITVPVARSVNPVTGQSWEVSGVAPSVT
VNPKESIAKAKSLISVRKTIPKAVRRVSDIIKRYYSFKDKIPALLNQLAKADYFTVVSEEDLAGKLNHEMQSVFEDPRLLIKATQVLTDDASSEDRSSSD
DLTDPLFKLEMISGNNGYLRFDRFPTPEVLLRLEDHIKKKIWQPVQETENLVIDLRFNTGGSTEALPILLSYMFDTSSSTYLFSIYDSIKNTTFDFHTLN
NISGPSYGSTKGVYVLTSYYTAEAGEEFAYLMQSLHRGTVIGEITSGMLLHSKTFQIEQTSLAITVPIINFIDVNGECWLGGGVVPDAIVLAEEALERAH
EIIAFHKNIQGLVQEAGDLLEKHYSVPEVAAKVSRLLQSKLTEGLYRSVVDYESLASQLTSDLQETSGDQRLHIFYCETEPETLHDTPKIPSPEEAGFIV
EALFKVDVMSGNIGYLRFDMMEDIKVLQAINPEFLKVVWNKLVNTDMLIIDVRYNTGGYSTAIPLLCTYFFDAQPLTHIYTLFDRSTATVTKVTTLPDVL
GQKYSSQKDVYILTSHITGSAAEAFTRTMKDLKRATVIGEPTIGGALSSGTYQIGNSILYASIPNQAVLNAVTGKPWSISGVEPHIVAQASDALIVAQKI
IATKQQKKNSGK* 0

>RBP3x_salSal Salmo salar transcript frag DY725143
EETAAKLGPLLRENIWTKVTHASSLIFDLRYSTAGELSGVPFIISYFSDPEPLIHIDTVFDRPSNTTKELWTMSSIMGERYGKRKDLIVLTSKRTMGAAEAIAYTLKHLNRAIIVGERSA
GGSVKVQKIRIGDSGFYITVPVARSVNPITGQSWEVSGVSPSVNINAKEAVANAKNLLAVRSAIPNAVQSVSDIIRQYYSFTDRVPALLQHLESTDFFSVISEEDLANKFNNELQSVSEDPRLMIKL

>RBP3_calMil elephantfish frag 2 domains 6-243,334-531
PPVTRESSPTSDKLPEDPTFLQALVDTVFKVSVLPDNTGYFRFDEFPEISVMSKLVQYIIEKVWLPVKDTDRLIVDLRHNVGGHSSVVPLLLSYFYDPEP
PVGLFTVYNRLTNTTSHTTLPGVGQHVYGSRKDIYVLTSHRTATAAEELAYLLQSLNRATIVGEITSGSLLHSRSFQIPSTHLVITIPFINFMDNHGECW
LGGGVVPDSIVLAEDTLERTKEIIGFHAQVAELVESTGKLLAVHYAIPEVAAEVSAVLSAKLTQGLYRSVVDWESLASRLTVDLQETSVWSVSGAEPHVI
VQANEAMTVALGIINLRAKIPSIFQAAGKLVADNYAFAQTGAGVAETIADLIEGTGYGMINTEGKLAEVLSDTLQQLSGDKHLKAVHIPGDSKHQTPGIAMIQ 0
0 QMPPPEILEDLVKFSYQTKVLENNVGYLRFDMFGDNEMITQVSELMAKHVWNVIASTSSLIVDLR 2
1 YNIGGPTSSIPILCSYFFDDDKTVLLDTVYSRPTDTISEMKAIPQVAGNGSTESSVHSYI 1
2  * 0

>RBP3_petMar lamprey exon3/4 fused, exon4 run-on, fixed genomic frameshift; four domains: 34-312,327-615,625-914,916-1217
0 MAGSREQRTAFSTRLLLLLLLPLATCPSQAPYKFDTAVVLHLAKVLLDNYCIPENLVGMDEAIQRAVDNGELLGVSDPESAASALTEGIQAALNDPRIAV
SYVAPPHTFEELLATIPQKTSFAVLDGNVGYLRADEIISEATIKKLGPVIVQRIWNRLVDTDTFVLDLRYNSHGDITGLPYLVSCFCEPRPVVHLDTVYY
RPTNESKEIWSLPDLQGARFAKHKDVFVLVSANTEGVAENVAYVLKHLHRATVIGEQTAGGSLEVERFRLGDSRFFVTVPTARSEPADRSWGVFPCVSAP
SERALDKALEILNARGVARKAVEAAGELLLSSYTFVERASAIADHLSWSEYGSVVSVEDLTSKLTQDLQSVAEDPRLVVSNREPEWVGAADPPGPPAPLP
DDEQMLEAIVDSAFKVEVLEGNIGYLRFDEFGDASAVMKLRKQLVSKVWERIHPTDDVIIDLRYNLGGSSTAIPIVLSYFQDVAPVHFYTVYDRLRNVTA
EFHTVSNLTSQLYGSKKGVYLLTSQHTATAAEEFTYLMQSLNRATIVGEITSGRLAHSLAFRLSDTGLYMTVPIVNFIDNNDEYWLGGGVVPDAIVLAENALDAAKEIIEFHAKMASL
LELAGALVEGYYAMLSDGENATAEILLKYREGWYRSVVDYEALASQLTSDLHEIWGDHRLHAFYSDLQIERMDEDKTPSVPSPEELSVLIDTVFKVDILANNVGYLRFDMMTDAEVLKHV
GPQLVEKVWNKISSTRSLVIDVRYNMGGYSTSIPILCSYFFDASPPRHLYTVFDRPSRSSTQVFTVPRVLGQRYGASKDVYILTSHMTGSAGEILTRVMSDLKRATVIGEPTAGGSLSTG
TYRIGDSRLYVFIPNQAGVSPSGGRTWSVAGVEPHVQTKASEALQSALRMVALRADAPSILRTVGKLVADGYSRAEAALGVPSKLAALLEAGEYGALRSEEELAFKLTVHLQLITGDRHL
KAVCVPEHATDRMPGIVPMQ 0
0 MPPTESFEDLIKFSFITDVLEGNIGYLRFDLFSDLEALEHVAHLLVEHVWKKICDTEILIIDLR 2
1 YNMGGYSTSIPILCSYFFDASPPRHLYTVFDRPSRSSTQVFTVPRVL 1^2 GQRYGASKDVYILTSHMTGSAGEILTRVMSDLKRATVIGEPTAGGSLSTGTYRIGDSRLYVFIPNQAGVSPSGGRTWSVAGVEPHVQTKASEALQSALRMVALRADAPSILRTVGKLVADGYSRAEAALGVPSKLAALLEAGEYGALRSEEELAFKLTVHLQLITGDRHLKAVCVPEHATDRMPGIVPMQVNVVRTRI* 0

>RBP3_braFlo Branchiostoma floridae Region: 9 exons 1 domain: 83-381 ClpP/crotonase e-38 419-630; misfused to PAPS sulfotransferase
0 MTRPSKVDIVFPIKPFTIPTAHEQVKGEGPVDINKNALCKSADEGHTHP 1
2 VSIAMAPTAYIVFVALVPTVLSVDWLDVVMGIGDVMADHYLDQDLRALNDQSLLQRWNRTLVHRFQ 0
0 SWSQDDMSDSLRMEEGLTSELRNITGDETIK 0
0 VWDFGVYENTTQEPVPREFYNFSTFVDNFK 2
1 KNREKHINVTMLEGNVGYVSIRSMSHIVDIILPDPEMTEFFLSKMAALNESK 0
0 AIILDLRYNLGGDREGVVHWASFFFNATPSVPLSDVYYRDGVNQYWTLLE 0
0 VPGGIRFPDMPLYLLTSNRTSREAEEFAYAMQVVNRTTIIGETT 1
2 AGEEFTGMWFPIDQTDVHLLTRTNVVRNPITQDSWSGK 1
2 GVTPDIIVPSEKALTVALRKIQGSEDTKMAASSGNIEPPRWTVYLVFICTSIAILTYPTFM* 0
</pre>

[[Category:Comparative Genomics]]