Updating blat servers: Difference between revisions

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(created page on how to edit the blat server lines in hgcentral)
 
(Replacing page with 'This page is no longer maintained.')
 
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* Do a select statement on dev to make sure that everything is where you expect it to be:
This page is no longer maintained.
<pre>
hgwdev: hgsql hgcentraltest
mysql> select * from blatServers where host="blat#" ORDER BY port;
+---------+--------+-------+---------+--------+
| db      | host  | port  | isTrans | canPcr |
+---------+--------+-------+---------+--------+
| apiMel1 | blat12 | 17778 |      1 |      0 |
| apiMel1 | blat12 | 17779 |      0 |      1 |
| monDom1 | blat12 | 17786 |      1 |      0 |
| monDom1 | blat12 | 17787 |      0 |      0 |
| caeRei0 | blat12 | 17790 |      1 |      0 |
| caeRei0 | blat12 | 17791 |      0 |      1 |
+---------+--------+-------+---------+--------+
</pre>
* Get DNA and protein sequences for a gene for given assembly and paste below for future reference:
:DNA
:protein
* Open 9 windows: BLAT DNA, BLAT protein, PCR for dev, beta, RR. Select the assembly of interest, put in DNA or protein as needed and hit submit. Make sure that all 9 windows function without errors.
<pre>
Getting into hgcentral database on dev, beta, RR:
 
DEV: hgwdev: hgsql hgcentraltest
BETA: hgwbeta: hgsql hgcentralbeta
RR: hgwdev: hgsql -h genome-centdb hgcentral
         
Statements for changing pointers:
       
update blatServers set host='blat#', port=177## where db='xxxXxx#' and isTrans=1;
update blatServers set host='blat#', port=177## where db='xxxXxx#' and isTrans=0;
 
**Note that the admins will simiply say:
bosTau3
trans port 17786
untrans port 17787
 
This means put the trans port number for isTrans=1 and the untrans port number for isTrans=0
</pre>
* Go to hgcentral on dev and change the pointers for the assembly of interest.
* Go back to the browser, hit back for the 3 windows open on dev, and then click re-submit. Check to see if broken. If not, go on; if yes, troubleshoot until solution is found.
* Repeat steps 4-5 for beta and the RR.

Latest revision as of 19:26, 10 March 2011

This page is no longer maintained.