Selenoprotein evolution: SECIS

Introduction to selenocysteine insertion into selenoproteins

The SECIS element in 3' UTR of selenoproteins allows the insertion of selenocysteine for the appropriate TGA codon at time of translation. Auxillary gene products are needed for this to happen, including a selenocysteine-specific translation elongation factor (eEFSec), Sec-tRNA[Ser]Sec, ribosomal protein L30, and a binding protein (SBP2).

The SBP2 protein has three domains, including a conserved L7Ae RNA binding motif for SECIS RNA binding, a not completely overlapping ribosome binding site and a redox-sensitive cysteine-rich domain. SBP2 shuttles between the nucleus and the cytoplasm via nuclear localization motif SELEQNESSKKNKKKKEKSKSSYEVLPVQE and nuclear export signal motifs, the latter using the CRM1 pathway.

The kink-turn tertiary structure in the SECIS element is at the core of binding recognition. A mutation in that of SEPN or in SB2 lead to rigid spine muscular dystrophy or abnormal thyroid hormone function, the latter through inadequate production of DIO2 needed to activate thyroid hormone T4.

A strong paralog of BP2 was noticed and briely studied earlier. That protein, KIAA0256, also has 17 coding exons with near-identical intron locations and phases and full-length alignability, establishing KIAA0256 and BP2 as resulting from an ancestral gene duplication. The percent identity overall is only 42% but reaches 72% (human to human) in the 3 exons defining the L7Ae kink-turn binding motif.

Since both genes can be recovered from chondrichthyes, lamprey, and amphioxus, the duplication event preceded the emergence of chordates. The roots of the selenoproteome and the insertion mechanism lie much deeper in pre-metazoa so it follows that the parent gene must have assumed the key SECIS recognition roles (as did both descendent genes, at least initially).

We could ask if KIAA0256 still plays a role in SECIS in contemporary organisms. One possibility is that some of the 30-odd selenoproteins preferentially use KIAA0256 over BP2, correlating to the two observed structural classes of SECIS elements (which needs to be revisited in the genomics era). Another possibility is differential use during development or in various cell types. It's worth noting that KIAA0256 is considerably more conserved across its length than BP2, which has only a few exons conserved back to fish) suggesting the latter has a more limited role.

A literature review establishes that DIO, GPX, and SEPP1 were generally taken as proxies for all selenoprotein SECIS elements in terms of establishing a role for BP2. Yet the timing of expansion of these and specialization of the former to thyroid during the period of gene duplication may have tainted their representativeness. Possibly many other selenoproteins utilize KIAA0256 at least to some extent. That would account for the greater conservation of KIAA0256 and the difficulty finding SECIS elements in expected position for certain selenoproteins, ie their elements may be adapted to a different receptor and so have low Cove scores to the extent that tool is based on the BP2 fold paradigm.

Ribosomal protein L30 appears also to bind the SECIS kink-turn and play a role in selenocysteine insertion despite its meagre length of 114 amino acids. Likely many ribosomal proteins, L30 has remarkable sequence conservation, here still 94% at human-lamprey.

L30 has weak homology and secondary structure parallels to the pertinent region of BP2 and KIAA0256. These latter proteins might be viewed as domain composites that have accreted the kink-turn binding domain long ago from this undoubtedly ancient ribosomal protein. This opens a window on the origin of selenocysteine insertion in early eukaryotes.

Reference set of 27 vertebrate SECIS BP2 L7Ae motif exons

>SECISBP2_homSap Homo sapiens (human) chr9 uc004aqj exons 1416
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEKIQSK 1
2 GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTVAARQAYKTMLENVQQELVGEPRPQAPPSLPTQGPSCPAEDGPPALKEKEEPHY 1

>SECISBP2_panTro Pan troglodytes (chimp)
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEKIQSK 1
2 GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTMAARQAYKTMLENVQQELVGEPRPQAPPSLPTQGPSCPAEDGPPALTEKEEPHY 1

>SECISBP2_macMul Macaca mulatta (rhesus)
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEKIQSK 1
2 GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTMAARQAYKTMLENVQQELAGEPRPQAPPSPPTQGPSCPAEDGPPALTEKEEPHY 1

>SECISBP2_otoGar Otolemur garnettii (bushbaby)
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKERRLVLGLREVLKHLKLKKLICVISPNCERQSK 1
2 GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTMAARQAYKTMLENVQRELAGEPGPQVPSSLPMEGPSCSVEDSPPAPTEKEEPHY 1

>SECISBP2_tupBel Tupaia belangeri (tree_shrew)
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRVVLGLREVLKHLKLKKLKCVIISPIZEKIQSK 1
2 GGLDDTLHTIIAYACAQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTMEARQAYRSMLESARQELAGEPGLQAPPQPPVQGPRASSEGSAPAPTGRQEPHC 1

>SECISBP2_musMus Mus musculus (mouse)
1 YCSQMLSKEVDACVTGLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKTQSK 1
2 GGLDDTLHTIIDCACEQNIPFVFALNRKALGRSLNKAVPVSIVGIFSYDGAQ 0
0 DQFHKMVELTMAARQAYKTMLETMRQEQAGEPGPQSPPSPPMQDPIPSTEEGTLPSTGEEPHY 1

>SECISBP2_ratNor Rattus norvegicus (rat) exons 1416
1 YCSQMLSKEVDACVTGLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKTQSK 1
2 GGLDDTLHTIIDCACEQNIPFVFALNRKALGRSLNKAVPVSIVGIFSYDGAQDQ 0
0 FHKMVELTMAARQAYKTMLETMRQEQAGEPGPQTPPSPPMQDPIQSTDEGTLASTGEEPHY 1

>SECISBP2_cavPor Cavia porcellus (guinea_pig)
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCIIISP 1
2 GLDDTLHTIIDYACAQNIPFVFALNRKALGRSLNKTVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTMAARQAYKTMLENVRQELAGEPRPQMPPDPPSEGPSSSLEDTAPDPSAEEPHY 1

>SECISBP2_oryCun Oryctolagus cuniculus (rabbit)
1 YCSQMLSKEVDACVTDLFKELVRFHDLMYQDPVKATTKCQFELRVGKALDHLRLKKLKCIIVFPKHKKQS 1
2 TIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTMAARQAYKTMLENMRHELAGEPGPPTPQPVQGPSCSAEDGPPAPTEGEVPHY 1

>SECISBP2_canFam Canis familiaris (dog)
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKIQSK 1
2 GGLDDTLHTIIDYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHRMVELTMAARQAYKTMLENVRQELAGEPGTPALANPPMQGLGCSTQDSPPAPTEKEEPHY 1

>SECISBP2_felCat Felis catus (cat)
1 YCSQMLSKEVDACVTDLLRELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKIQSK 1
2 GGLDDTLHTIIGYACEQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHRMVELTMAARQAYKTMLENARQELAGEPGPPAPGSPPPQPPAPAGRDEPRY

>SECISBP2_equCab Equus caballus (horse)
1 YCSQILSKEVDACVTELLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLRKLKCIIISPNCEKIQSK 1
2 GGLDDTLHTIIDYACEQNIPCVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTKAARQAYKAMLENVHQELAGEPGPQAPASPPAQGPSCSTEGAPPAPTGKEEPHY 1

>SECISBP2_bosTau Bos taurus (cow)
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKAKRRLVLGLREVLKHLKLRKLKCIIISPNCEKIQSK 1
2 GGLDDTLHTIIDYACDQNIPFVFALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTMAARQAYRTMLENARQELPGELGPCAPVGPPSQGPGCPVEDSPLAPTEKEEPHY 1

>SECISBP2_eriEur Erinaceus europaeus (hedgehog)
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCIIISPNCEKIQSK 1
2 GGLDETLHTIIDCACEQNIPFVFALNRKALGRSLNKGVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTMAARQAYKALLENMRQELAEESGSPAPSSPPVQSPSEDGPPAPAEKEEPHY 1

>SECISBP2_dasNov Dasypus novemcinctus (armadillo)
1 YCSQVLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEKIQSK 1
2 GGELDDTLHTIIDYAASRHSICVALNRKALGRSLNKAVPVSVVGIFSYDGAQ 0
0 DQFHKMVELTMAARQAYKAMLENVRKELAGEPGPRSPPSPPALGPHSSAGDVHPTSAGKEEPHY 1

>SECISBP2_loxAfr Loxodonta africana (elephant)
1 YCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRLVLGLREVLKHLKLKKLKCVIISPNCEKIQSK 1
2 GGLDDTLHTIIDYACEQNIPFVFALHRKALGRSLNKPVPVSVVGIFSYDRAQ 0
0 DQFHKMVELTMAARQEYKTMLESVRQELAEEPRAGSPPSPPTQGPGCSAEVPRPAPTEKEEPRY 1

>SECISBP2_monDom Monodelphis domestica (opossum)
1 YCSQMLSKEVDDCVMDLLKELVRFQDRMYQKDPVKAKTKRRLVMGLREVLKHLKLKKLKCVIISPNCEKSKSK 1
2 GGLDETLHTIIDYACEQNVPFVFALNRKALGRSVNKVVPVSVVGIFSYDGAQ 0
0 DQFHKMIALTMEARQAYKIMLSTLKEEPALETENPPSPSLPRPSESCPSELGQTDPTQEEEPNY 1

>SECISBP2_triVul Trichosurus vulpecula (possum)
1 YCSQMLSKEVDDCVMDLLKELVRFQDRMYQKDPVKAKTKRRLVMGLREVLKHLKLKKLKCVIISPNCEKSKSK 1
2 GGLDETLHTIIDYACEQNVPFVFALNRKALGRSVNKVVPVSVVGIFSYDGAQ 0
0 DQFRKMIELTMEARQAYKVMLATLKEGAEALQTENPLPTSLTPQGQGCSSELSKTTDPTKEEEPNY 1

>SECISBP2_galGal Gallus gallus (chicken)
1 YCSQVLSKEVDSCVTDLLKELVRFQDRLYQKDPVKAKIKRRLVMGLREVLKHLRLKKLKCVIISPNCEKIQSK 1
2 GGLDETLHNIIDCACEQNIPFVFALNRKALGRCVNKAVPVSVVGIFSYDGAQ 0
0 DHFHRMVQLTTEARKAYKDMVAALEEELKELSKPLNZKSCLSETGKTSSTKEDIPNY 1

>SECISBP2_anoCar Anolis carolinensis (lizard)
1 YCTQVLSKEVDSCVTDLLKELVRFQDRLYQKDPVKAKTKRRLVMGLREVLKHLKLKKLKCVIISPNCEKIQSK 1
2 GGLDETLHLIIDSACEQNIPFVFALNRKALGRCLNKAVPVSVVGIFSYDGAQ 0
0 DYFHKMVELTMEARQAYKDMISALERELKKKTVRKKPLQSRPLDTVEASSTEEDVPDY 1

>SECISBP2_xenTro Xenopus tropicalis (frog) NM_001097262
1 YCSQVLSKDVDNCVMELLKELVRFQDRLFLKEPAKAKSKRRLVMGLREVLKHLKLQKLKCIIISPNCEKIQSK 1
2 GGLDDTLQTIISHACEQNVPFVFALNRKALGRCLNKAVPVSVVGVFSYDGAQ 0
0 DHFHKLCELTVQARQAYKDMIAAAQEQQSETEAGKNEEDPVAVNGQNKSDDMREESKAEEPDEPNY 1

>SECISBP2_danRer Danio rerio (zebrafish)
1 YCNQVLSKDVDECVSNLLKELVRFQDRLYQKDPMKARMKRRLVMGLREVLKHLKLKKVKCVIISPNCERIQSK 1
2 GGLDEALHNIIDTCRDQSVPFVFALSRKALGRCVNKAVPVSLVGIFNYDGAQ 0
0 DFYHKMIELSSEARTAYEVMLLNLEQTDAEEAQQTSPLAEKVETSSGDPQPEEPEY 1

>SECISBP2_tetNig Tetraodon nigroviridis (pufferfish)
1 YCNQVLSKEIDESVTLLLQELVRFQERVYQKDPTKAKSKRRLVMGLREVTKHMKLQTIKCVIISPNCEKIQAK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYSGAE 0
0 DFYHKMIELSSEARIAYEVMLSNLEQTSAEEEPQTCTLAEKINTSSEDAQPEEPEY 1

>SECISBP2_takRub Takifugu rubripes (fugu)
1 YCTQMLSKDVDECVTTLLKELVRFQDRLYQKDPIKARMKRRIVMGLREVQKHLKLRKLKCVIISPNCERIQSK 1
2 GGLDEALHTIIDTCREQAVPFVFALSRRALGRCVNKAVPVSLVGIFNYDGAQ 0
0 DFYHKMIELSSEARTAYEVMLLNLEQTDAEEAQQTSPLAEKVETSSGDPQPEEPEY 1

>SECISBP2_gasAcu Gasterosteus aculeatus (stickleback)
1 YCNQVLSKEIDESVTMLLQELVRFQERIYQKDPTKAKTKRRLVMGLREVTKHMKLNKIKCVLISPNCEKIQAK 1
2 GGLDEALYNVIAMARDQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYSGAE 0
0 DFYHKMIELSSEARRAYEVMVSSLEQTGQADPESVEEKLQISSAAEEAELGRDITPPEEPEY 1

>SECISBP2_oryLap Oryzias latipes (medaka)
1 YCSQMLRKDVDECVTVLLKELVRFQDRLYHKDPIKARMKRRLVMGLREVLKHLKLRKVKCVIISPNCEQIQSK 1
2 GGLDEALHTIIQTCREQAVPFVFALSRKALGHCVNKAVPVSLVGIFNYDGAQ 0
0 DHYHKMIELSAEARKAYEVLVSSLERDQQEESHPDRGTCFGSVTAEPEKPHY 1

>SECISBP2_calMil Callorhinchus milii (elephantfish) AAVX01044988
1 YCSQVLSKDVDSCVTDLLKELVRFQDRLYQKDPIKAKKKRRIVMGLREVLKHLKLKRLKCIIISPNCEKIQSR 1
2 GGLDDALHNIISIACEQEIPFVFALNRKALGQCVNKPVPVSVLGIFSYDGAE 0
0 NQFHQMVEITEEARKAYQEMLDALQQELEADEEKGDSEEQPLISSESSTIHFNNVTSQPFSEADEPEY 1

>SECISBP2_braFlo Branchiostoma floridae (amphioxus) extra exon
1 YCNQVLDKEIDATVTMLLQDLVRFQDRQYHK 00 DPIKAKAKRRIVMGLREVTKHLKLRKLKCIIIAPNLEKIQSK 1
2 GGLDDAIETILNLCMEQDVPFVFALGRKALGRAVNKLVPVSVVGVFNYDGAE 0
0 1

Reference set of 22 deuterostome SECIS KIAA0256 L7Ae motif exons

>KIAA0256_homSap Homo sapiens (human) uc001zxd chr15 exons 14-16 75% 69% 51%
1 YCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
0 SLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_panTro Pan troglodytes (chimp)
2 YCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
0 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
1 SLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_macMul Macaca mulatta (rhesus)
2 YCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
0 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
1 SLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_tupBel Tupaia belangeri (tree_shrew)
1 YCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
0 SLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_musMus Mus musculus (mouse)
0 YCNQVLSKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
1 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
2 SLFNRLVELTEEARKAYKDMVAATEQEQAEEALRSVKTVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_ratNor Rattus norvegicus (rat)
2 YCNQVLSKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
0 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
1 SLFNRLVELTEEARKAYKDMVAATEQEQAEEALRSVKAVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_canFam Canis familiaris (dog)
1 YCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
0 SLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_equCab Equus caballus (horse)
1 YCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
0 SLFNKLVALTEEARRAYKDMVAALEQEQAEEASKNVKKGPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_dasNov Dasypus novemcinctus (armadillo)
1 YCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSKG 1
2 GLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
0 SLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_monDom Monodelphis domestica (opossum)
0 YCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVKAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
1 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYCGAE 0
2 SLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_galGal Gallus gallus (chicken)
1 YCNQVLSKEIDECVTLLLQELVSFQERIYQKDPMRAKARRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYSGAE 0
0 DLFNKLVSLTEEARKAYRDMVAAMEQEQAEEALKNVKKAPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_anoCar Anolis carolinensis (lizard)
1 YCNQVLSKEIDECVTLLLQELVSFQEQIYQKDPMRAKAKRRLVMGLREVTKHMKLSKIKCVIISPNCEKIQSK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYSGAE 0
0 NLFNKLVSLTEEARKAYRDMVAAMEQEQEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_xenTro Xenopus tropicalis (frog)
1 YCNQVLSKEIDECVTVLLQELVSFQERVYQKDPVKAKSKRRLVMGLREVTKHMKLNKIKCVIISPNCEKIQSK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFSYSGAE 0
0 SLFHNLVSLTEEARKAYKDMVSSMEQEQAEEALKNIKKVHMGHSRNPSAASAISFCSVISEPISEVNEKDY 1

>KIAA0256_danRer Danio rerio (zebrafish)
1 YCNQVLSKEIDESVTLLLQELVRFQERVYQKEPSKAKAKRRLVMGLREVTKHMKLHKIKCVIISPNCEKIQAK 1
2 GGLDEALHNIIDTCRDQSVPFVFALSRKALGRCVNKAVPVSLVGIFNYDGAQ 0
0 GLFNKLVSLTEEARRAYKEMVSALEQEQAEEALKNVKKVPHHMGHSRNPSAASAISFCSVISEPISEVNEKEY 1

>KIAA0256_tetNig Tetraodon nigroviridis (pufferfish)
1 YCNQVLSKEIDESVTLLLQELVRFQERVYQKDPTKAKSKRRLVMGLREVTKHMKLQTIKCVIISPNCEKIQAK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYSGAE 0
0 SLFNQLVSLTEEARKAYKDMVSALEQEQTEEALKNEKKVPHQMGHYRNHSAASAVSFCSIFSEPISEVNEKEY 1

>KIAA0256_takRub Takifugu rubripes (fugu)
1 YCNQVLSKEIDESVTLLLQELVRFQERVYQKDPTKAKSKRRLVMGLREVTKHMKLQTIKCVIISPNCEKIQAK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNFSGAE 0
0 SLFNQLVSLTEEARKAYKDMVSALEQEQTEEALKNEKKVPHQMGHYRNHSAASAVSFCSIFSEPISEVNEKEY 1

>KIAA0256_gasAcu Gasterosteus aculeatus (stickleback)
1 YCNQVLSKEIDESVTMLLQELVRFQERIYQKDPTKAKTKRRLVMGLREVTKHMKLNKIKCVLISPNCEKIQAK 1
2 GGLDEALYNVIAMARDQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYSGAE 0
0 GLFNRLVSLTEEARKAYKDMVSALEQEQAEEAQKNDKKLPHHMGHSRNHSAASAISFCSIFSEPISEVNEKEY 1

>KIAA0256_oryLap Oryzias latipes (medaka)
0 YCNQVLSKEIDESVTLLLQELVRFQERVYQKDPSKAKSKRRLVMGLREVTKHMKLHKIKCVIISPNCEKIQAK 1
1 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYSGAE 0
2 GLFNQLVSLTEEARKAYKEMVSALEQEQAEEALKHDKKVPHHMGHSRNHSAASAISFCSILSEPISEVNEKEY 1

>KIAA0256_calMil Callorhinchus milii (elephantfish) AAVX01105236
1 YCNQVLSKDIDECVTLLLQELVRFQERVYQKDPIKAKMKRRLVMGLREVTKHMKLRKIKCVIISPNCEKIQSK 1
2 GGLDEALYNVIAMAREQEIPFVFALGRKALGRCVNKLVPVSVVGIFNYFGAE 0
0 1

>KIAA0256_petMar Petromyzon marinus (lamprey)
1              LHKLRALIISPNCEKIQAK 1
2 GGLDEALQTVIALASEQSVPFVFALNRKALGHCLNKKVPVSVVGVFHYGGAE 0
0 THFQRLVALTEEARSAYRNMVSSLQRQEAAATSEPTGHTEDPLEASASPPSVPAHDPTALLHLLRPQQGPREDDPAEASGRSPGRNA 1

>KIAA0256_cioInt Ciona intestinalis (tunicate)
1 YCCQVLDKRVDEMSNQMLQRLVYFQDR 21 RLYKTDPAKAKRKRRVVLGFREVTKHLKMKKLRCVIISPNLEKIESK 1
2 GGLDDVLHEILDLCKEQNIPYVFALGKKALGRAVSKTVPVSIVGVFDYSGAE 0
0 1

>KIAA0256_strPur Strongylocentrotus purpuratus (sea_urchin)
1 YCNQVLDKDIDGCCTTLLQTLVKFQDRQYHKDPAK 00 AKMKRRLVMGLREVTKHLKLKKIKCVVVSPNLERIQSK 1
2 GGLDEAMDRISSLASEQNVPLIFALGRKALGRAVNKVVPVSVVGIFNYDGAE 0
0 DTYKQLLDLSTRARNAYADMVRKFQQELEAANAASAARMAKHRHHMGHNRNLFKG 1

Reference set of 10 deuterostome ribosomal L30 L7Ae motifs

>L30_homSap Homo sapiens (human) 4 exons numerous pseudogenes
0 MVAAKKT 0
0 KKSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALR 2
1 KSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDP 1
2 GDSDIIRSMPEQTGEK* 0

>L30_tupBel Tupaia belangeri (tree_shrew)
0 MVAAKKT 0
0 KKSLESINSQLQLAMKDGKYVLGYKQTLKMIRQGKAKLVILANNCPALR 2
1 KSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRACTLAIMDP 1
2 GDSDIIRSMPEQTGEK* 0

>L30_ratNor Rattus norvegicus (rat) Sep15 Gpx4 Gpx1 Dio1 quite weak homology 35% with BP2 exons
0 MVAAKKT 0
0 KKSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALR 2
1 KSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDP 1
2 GDSDIIRSMPEQTGEK* 0

>L30_myoLuc Myotis lucifugus (microbat)
0 MVAAKKT 0
0 KKSLESINSRLQLVMKSGKYLLGYKQTLKMIRQGKAKLVILANNCPALR 2
1 ISEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDP 1
2 GDSD-IRSMPEQTGEK* 0 

>L30_echTel Echinops telfairi (tenrec)
0 MVAAKKT 0
0 KNSLESINSRLQLVMKSGKYMLGYKQMLKMIRQGKAKLVVLANNCPALR 2
1 KSEIEYYAMLAKTGVHHYSGHNIELGTACGKSCRVCTLAITDP 1
2 GDADIIRSMPEQTGEK* 0

>L30_anoCar Anolis carolinensis (lizard)
0 MVAAKKT 0
0 KKSLESINSRLQLVMKSGKYVLGYKQTLKMIQQGKAKLVILANNCPALG 2
1 KSEIEYYAMLAKTGVHHYSGNNIEMGTACGKYYRVCTLAIIDP 1
2 GDSDIIRSMQEQTAEK* 0

>L30_danRer Danio rerio (zebrafish) 94%
0 MVAAKKT 0
0 KKSLESINSRLQLVMKSGKYVLGYKQSQKMIRQGKAKLVILANNCPALR 2
1 KSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDP 1
2 GDSDIIRSMPDQQQGGEK* 0

>L30_squAca Squalus acanthias (spiny dogfish) 97%
0 MVAAKKT 0
0 KKSLESINSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALR 2
1 KSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDP 1
2 GDSDIIRSMPEQISEK* 0

>L30_petMar Petromyzon marinus (lamprey) 94%
0 MSAKKT 0
0 KKAIESINSRLQLVMKSGKYCLGYRQTLKMIRQGKAKLVLLANNCPALR 2
1 KSEIEYYAMLAKTGVHHYSGNNIEMGTACGKYYRVCTLAIIDP 1
2 GDSDIIRSMPEQQQPQPGDK* 0

>L30_braFlo Branchiostoma floridae (amphioxus) 84% to homSap
0 MKQK 0
0 RKTMESINSRLQLVMKSGKYVLGLKETLKVLRQGKAKLIIIANNTPALR 2
1 KSEIEYYAMLAKTGVHHYSGNNIELGTACGKYFRVCTLAITDP 1
2 GDSDIIRSMPAEDKGESK* 0

Positioning of SECIS elements in 3' UTR

..................

Reference sets of vertebrate SECIS elements

SEPP2: 5 SECIS sequences

>SEPP2_SECIS_monDom Monodelphis domestica (opossum) chr2:9455773-9456962 COVE: 30 55 bp after stop codon
ACGTGCCGCTGCCCCTCCCTCCCTCCAAGAATGACGCCCACAGTGAAACCCAGAGAACTGGTCCCTGTGGGCTGATGCCCCAGAGGGGAGGAGAGGC

>SEPP2_SECIS_macEug Macropus eugenii (wallaby) SECIS COVE score: 26.38 trace ti|975557399 contains terminal exon
CCTCATTCCTCTTCTCCATGGCATCAGCCCACCATGGCCGGTTCCCCGGGTTTCACTGTGGGCATCATTAATTGAGGGAGGGAAGGGCAATGGGCAAG

>SEPP2_SECIS_ornAna Ornithorhynchus anatinus (platypus)Ultra131:321101-348720 COVE: 34.28 no match to chicken
TCCCTCGACTCCCGCTCCCGCCTCGCACTCATGACGTCCACGGTGTCAACCGGCCCGCCGGGCACCGTGGACTGACGCCGGTCGAGGCGGAGGGGT

>SEPP2_SECIS_ornAna Ornithorhynchus anatinus (platypus) Ultra131:321101-348720 COVE: 22.82 no match to chicken
GGAATCAGGAACCCAGTAACATGAGGTCATCTTCGGAAGCCTGTGCCTAGAGGACCAAGATAATGGAAAAAGTGACGGACAAGGGTGTGTAGCTGG

>SEPP2_SECIS_tetNig Tetraodon nigroviridis (pufferfish) COVE: 17
GCTGGACCCAGGCTGCTGGTGGTCCCGTTGATGACGTCTGCGCTGGTAAACCTGCCTGCAGGAGCCTGTGGACCGACGTGTGTGGACCCACCGGCAG

SELI: 17 SECIS sequences

>SELI_SECIS_homSap Homo sapiens (human) COVE score: 21.61
UUUCACUGAA UGAAGUU UGUGCUUGA AUGAA GAGUGUAUCUUA AA CCCCCUUUUUUUGGA CAGGCUGCACUU GGAU AAAAUA GGCACCA CUGUGUUGAU

>SELI_SECIS_panTro Pan troglodytes (chimp) COVE score: 21.61
uuucacugaa ugaaguu ugugcuuga augaa gaguguaucuua aa cccccuuuuuuugga caggcugcacuu ggau aaaaua ggcacca cuguguugau

>SELI_SECIS_macMul Macaca mulatta (rhesus) COVE score: 21.25
uuucacugaa ugaaguu ugugcuuga augaa gaguguaucuua aa ccuccuuuuuuugga caggcugcacuu ggau aacaua ggcacca cuguguugau

>SELI_SECIS_otoGar Otolemur garnettii (bushbaby) COVE score: 21.25
uuucacuaag ugaaguu ugugcuuga augaa gaguguaucuu aa acccuuuuuuuuggac agguugcacuu ggau aaaaua ggcacca uuguguugau

>SELI_SECIS_musMus Mus musculus (mouse) COVE score: 24.62
uuccacugaa ugaaguu ugugcuuaa augaa gagugugucuu aa acccuuuuuuuuggac agguugcacuu ggau aaaaua ggcacca cuguguugau

>SELI_SECIS_ratNor Rattus norvegicus (rat) COVE score: 24.62
uuccacugaa ugaaguu ugugcuuaa augaa gagugugucuu aa acccuuuuuuuuggac agguugcacuu ggau aacaua ggcaccg cuguguugau

>SELI_SECIS_cavPor Cavia porcellus (guinea_pig) COVE score: 17.42
uuccagugaa ugaaguu ugugcuuga augaa gaguguaucuua aa cccuuuuuuuuuugga cagguugcacuu ggau aaaaua ggcacca cuguguugau

>SELI_SECIS_oryCun Oryctolagus cuniculus (rabbit) COVE score: 19.47
ucuacugaau gaaguuu gugcuuga augaa gaguguaucuua aa cccuuuuuuuuugga cagguugcacuu ggau aaaau aggcac cacuguugau

>SELI_SECIS_sorAra Sorex araneus (shrew) COVE score: COVE score: 8.13 loose
uuccacugaa ugaaguu ugugcuuga augaa gaguguaucuu aa acccuuuauuuuguuuuuuugga cggauuacacuu ggau auaaua ggcacca uucuguugau

>SELI_SECIS_eriEur Erinaceus europaeus (hedgehog) COVE score: 14.43 loose
cacggaauga aguaugu gcuuga augaa gaguguaucuu aa acccuuuuuuuuuuuugga cagguugcacuu ggau aua auaggc accacucugu

>SELI_SECIS_canFam Canis familiaris (dog) COVE score: 14.04
uuccacugaa ugaaguu ugugcucga augaa gaguguaucuua aa cccuuuuuuuuuugga uggauugcacuu ggau aaaaua ggcacca cuguguugau

>SELI_SECIS_equCab Equus caballus (horse) COVE score: 19.57
uuucacugag ugaaguu ugugcucga augaa gaguguauucuu aa acccuuguuuuugga uggauugcacuu ggau aaaaua agcacca cuguguugau

>SELI_SECIS_dasNov Dasypus novemcinctus (armadillo) COVE score: 17.76 loose
cuacugaauu aaguuug ugcuuga augaa gaguguaucuu aa acccuuuguuuuuuugga cagguugcacuu ggau aaaa uaggca ccacuauguu

>SELI_SECIS_loxAfr Loxodonta africana (elephant) COVE score: 25.81
uuccacugaa ugaaguu ugugcuuga augaa gaguguaucuu aa acccuuuuuuuuggac agguugcacuu ggau aaagua ggcacca cuguguugau

>SELI_SECIS_monDom Monodelphis domestica (opossum) COVE score: 26.46
uucuacugaa ugcaauu ugugcuuga augaa gagugugucuu aa auccuuuauauggac aggcugcacuu ggag aga auaagca caaccauguu

>SELI_SECIS_ornAna Ornithorhynchus anatinus (platypus) COVE score: 19.25
ucccagugaa ugaagcu ugugcuuga augaa gagugcaucuua aa cccauuuuuuuugga aaagcugcacuu ggag agaaag ggcacg acuguguuua

>SELI_SECIS_anoCar Anolis carolinensis (lizard) COVE score: 22.02
uccccuuugu guguguc cuuugugcgugu augaa gagugcggccuc aa cccaggcgucuugga agggccgcaccc ggaa gaa acggagc acagcaaaga

>SELI_SECIS_galGal Gallus gallus (chicken) COVE score: 24.62
uuuuauugag uuuauuu gugcuuaa augaa gagugcgcuuc aa acccagaccaggag agggcgcacuu ggag ugagc gagucaa accuugcucc

SELK: 19 SECIS sequences

>SELK_SECIS_homSap Homo sapiens (human) COVE score: 34.53
acaaggacug cucugug uccucacagauga augag gucaugcuggg aa uucccucugcaggga acuggccugac ugac augcaguuc cauaaa ugcagauguu

>SELK_SECIS_panTro Pan troglodytes (chimp) COVE score: 34.53 
acaaggacug cucugug uccucacagauga augag gucaugcuggg aa uucccucugcaggga acuggccugac ugac augcaguuc cauaaa ugcagauguu

>SELK_SECIS_macMul Macaca mulatta (rhesus) COVE score: 36.14 
acaaggacug cucugug uccucacagauga augag gucaugcuagg aa uucccucuacaggga acuggccugac ugac augcaguuc cauaaa ugcagauguu

>SELK_SECIS_otoGar Otolemur garnettii (bushbaby) COVE score: 31.99
caaggauugc ucugugu cuucacagauga augag gucaggcuggg aa uucucucuucaggga acuggccugac ugac augcagu ucuauaa acgcacuuuu
 
>SELK_SECIS_tupBel Tupaia belangeri (tree_shrew) COVE score: 36.65
acaaggauug cucugug uccccacagauga augag guuaugccggg aa uucccuccacaggga ucuggccugac ugau acgcaguuc uauaaa ugcacauguu

>SELK_SECIS_musMus Mus musculus (mouse) COVE score: 31.73
acaaggauug cucugug uccccacagauga augag gucaugcuggg aa uucccucugcagga ucuagccugac ugau acgcaguuc uauaaa uguacauguu
 
>SELK_SECIS_ratNor Rattus norvegicus (rat) COVE score: 33.02
acaaggacug cucugug uccucacagaaga augag gucaugcuggg aa cucccucugcagga ucuggccugac ugau gugcaguuc uauaaa uguacaugug
 
>SELK_SECIS_cavPor Cavia porcellus (guinea_pig) COVE score: 33.72
cucuguguuc ucacaga uaa augag gucacgccagg aa uucucucagcaggga ucuggcuugac ugau acgcagu ucucua aaugcauaug
 
>SELK_SECIS_oryCun Oryctolagus cuniculus (rabbit) COVE score: 38.80 
accaggauug cucugug uccccacagauga augau gucaggcuggg aa uucccuccacaggga ucuggccugau ugag augcaguuc uauaaa ugcguauguu

>SELK_SECIS_sorAra Sorex araneus (shrew) COVE score: 40.91
auaaggacug uucugug uccacacagauga augag gucaugcuggg aa uucccucuacgggga ucuggcaugac ugau augcag uucgaua aaugcacaug

>SELK_SECIS_eriEur Erinaceus europaeus (hedgehog) COVE score: 37.30
acaaggauug cucugug uucucacagguga augag guuaugcuggg aa uucccuccaugggga ucuggcaugac ugau augcaguuc uauaaa ugcacauguu

>SELK_SECIS_canFam Canis familiaris (dog) COVE score: 36.30 
acaaggauug cucugug ccuucacagacgg augag guugugcuagg aa uucccuccccaggga ucuggcaugac ugac augcaguuc uauaaa ugcacauguu

>SELK_SECIS_felCat Felis catus (cat) COVE score: 37.35
acgagaauug cucugug uccucacagacag augag gucgugcuggg aa uucccuccccaggga ucuggcaugac ugac augcaguuc uauaaa ugcacauguu

>SELK_SECIS_bosTau Bos taurus (cow) COVE score: 39.16 
cucugugucc ucacaga cga augag gucaugcuggg aa uucccuccgcaggga ucuggcaugac ugac augcagu ucuaua aaugcacguu

>SELK_SECIS_dasNov Dasypus novemcinctus (armadillo) COVE score: 29.00
acaaggauug cucugug uccucacagauga augag gucauguuuggg aa uucccucugcaggg aucuggcaugac ugac uugcaguuc cauaaa ugcacauguu

>SELK_SECIS_loxAfr Loxodonta africana (elephant) COVE score: 34.85 
acaaagacug cucugug uccccacagacgg augag gcugugcuggg aa uucccucugcaggga ucuggcauggc ugac augcaguuc cauaaa ugcacauguu

>SELK_SECIS_monDom Monodelphis domestica (opossum) COVE score: 21.75
aagaauugcu cugucua cacagauua augau guugugcuggg aa cucccaucuuacagga uccaguguaac ugau ugcaa uuguaua aaugcacaug

>SELK_SECIS_ornAna Ornithorhynchus anatinus (platypus) COVE score: 33.68
aauaauugug cugugaa caagcagauua augau guuuugcuggg aa uuccuucaggga uccaguauaac ugau aaagc aauuaua uaaaggcaca

>SELK_SECIS_anoCar Anolis carolinensis (lizard) COVE score: 33.87
aauucccugc ucugcca auuggcgggacc augau guuguccuggg aa uuccuuauucuggga uccagggcaac ugaa aagcaguuc uguuaa auuaaaugca

>SELK_SECIS_galGal Gallus gallus (chicken) COVE score: 30.90
ugagaacugu ucugcaa uauaagcagauga augaa guuguacuggg aa cuccuucaagga uccaguguaac ugaa gugcagug uuauuaa auacauguuu

GPX3: 15 SECIS sequences

>GPX3_SECIS_homSap Homo sapiens (human) COVE score: 27.80
ACGGACCCCA UGGCAGG GGUGGCGUCUUC AUGAG GGAGGGGCCCA AA GCCCUUGUGGGC GGACCUCCCC UGAG CCUG UCUGAG GGGCCAGCCC

>GPX3_SECIS_panTro Pan troglodytes (chimp) COVE score: 27.80  
cacguacccc augucgg ggguggugucuuc  augag  ggaggggccca  aa  gcccuugugggc ggaccucccc ugag ccugu cugagg ggccagcccu

>GPX3_SECIS_macMul Macaca mulatta (rhesus) COVE score: 27.80 
cacguacccc augucag ggguggcgucuuc  augag  ggaggggccca  aa  gcccuugugggc ggaccucccc ugag ccugu cugagg ggccagcucu

>GPX3_SECIS_tupBel Tupaia belangeri (tree_shrew) COVE score: 29.38 
ccacaucccu gugucag ggguggcaucucc  augag  ggaggggcccg  aa  gcccuuguggg cggaccucccc ugag ccugu cugagg ggccggcccu

>GPX3_SECIS_musMus Mus musculus (mouse) COVE score: 33.37 
cguguacccc aggucag ggguggugucucu  augaa  ggaggggcccg  aa  gcccuuguggg cgggccucccc ugag cccgu cuguggu gccagcccuu
 
>GPX3_SECIS_ratNor Rattus norvegicus (rat) COVE score: 33.37 
caugugcucc aagucag ggguggugucucc  augaa  ggaggggcccg  aa  gcccuuguggg cgggccucccc ugag cccgu cuguggu gccagcccuu
 
>GPX3_SECIS_canFam Canis familiaris (dog) COVE score: 29.96
cauguucccc gugucag gaauggcaucucc  augaa  ggaggggccc  ga  agcccucaugggc ggaccucccc ugag ccugu cugaag ggccggcccu

>GPX3_SECIS_felCat Felis catus (cat) COVE score: 30.08 
gccacguccc gugucag ggauggcgucucc  augaa  ggaggggccc  ga  agcccuugugggc ggaccucccc ugag ccugu cugaag ggccagcccu

>GPX3_SECIS_equCab Equus caballus (horse) COVE score: 26.20 
uacauucccc augucag ggguggcaucucc  augau  ggaggggcccg  aa  gcccugguggg cggaccucccc agag ccugu cugaag ggccagcccu

>GPX3_SECIS_bosTau Bos taurus (cow) COVE score: 30.87 
acguuccccg ugucagg gggcggcaucgcc  augaa  ggaggggcccg  aa  gcccgcguggg cgggccucccu ugag ccu gucugag gggccagccu

>GPX3_SECIS_loxAfr Loxodonta africana (elephant) COVE score: 16.32 
cuacgucccc gugucaa gagcggcaucucc  augau  gguggggcccg  aa  gccccugugg cggaccucccc agag ccugucc caugggc cagcccuuag

>GPX3_SECIS_echTel Echinops telfairi (tenrec) COVE score: 12.78 
ggcugugucc cgugcag agggagcaucucc  augag  ggugaggcccg  aa  gcccccgugg cggaccucgac ccgag ucug cucugg gccugccuuc

>GPX3_SECIS_monDom Monodelphis domestica (opossum) COVE score: 22.94
ugaacauaag ggauggc aucucu  augau  ggugggaucca  aa  gccucuucaggg cggguuccauc agag ccugcaaaa gguguc aggacccuua

>GPX3_SECIS_ornAna Ornithorhynchus anatinus (platypus) COVE score: 31.33
ugucccagaa uggggag guggcaucauc  augac  agcggggucug  aa  agccccuccugga uggaccccgcc cgaa ccug cucggcg guggcaugac

>GPX3_SECIS_anoCar Anolis carolinensis (lizard) COVE score: 28.20 
GAGAUUUUGG GCCAAGG AAGUUGCAUCACU  AUGAG  GGUUAGGUCUG  AA  AGCUCCCAAAAAGAG CGGACCUAGCC UGAG GCUGCAAA GCUCUGGU GUAGCCCUUU

>GPX3_SECIS_galGal Gallus gallus (chicken) COVE score: 30.65
GCCCUGGGGA GCAGAGG AUGACAUCUCC  AUGAA  GGCCUGGCCUG  AA  AGCCCCCACCAUGGGG UGGGCUCGGCC CGAU CCCG CCCAGGC GCGGUGCAGC 

GPX6: 10 SECIS sequences (most species have selenocysteine

>GPX6_SECISa_homSap Homo sapiens (human) chr6:28,578,901-28,580,158 Cove score: 18.10
cccaccucac augaagg gaagggcaucucc  augau  gguggauccc  aa  aaccccucuggguc gcacccugcc agag ccu uccuug gugccugucc
 
>GPX6_SECIS_panTro Pan troglodytes (chimp) COVE score: 23.45
cccaccucac augaagg gaggggcaucucc  augac  gguggguccc  aa  aaccucucgggguc ggacccugcc agag ccu uccuug gugccugucc
 
>GPX6_SECIS_macMul Macaca mulatta (rhesus) COVE score: 21.81 
accucauuca cagaagg gaggggcaucuuu  augau  ggugggucuc  aa  aaccucucuggguc agacccuacc agag ccu uccuug gugccugucc

>GPX6_SECIS_cavPor Cavia porcellus (guinea_pig) AAKN02051715 Cove score: 19.91
AGAAGCCUCC CUAGAAG GAAGGGUGUCUCC  AUGAU  GGUGGGUCCC  AA  AAGCCCUGGAUC GGACCCUACC AGAA CCU UCCCUGG UGCCUGUCCU

>GPX6_SECIS_canFam Canis familiaris (dog) Cove score: 24.33 but not found in 28way
CCUGGCCUCA UAUGAAA GGAGGGCAUCUCC  AUGAU  GGUGGGUUCC  CA  AGCCCCUGCGGUC GGACCCUACC AGAG CCU UCUUGG GUGCCUGUCC

>GPX6_SECIS_equCab Equus caballus (horse) COVE score: 25.00
uucaucucac augagcu gaggggcaucucc  augau  gguggguccc  aa  agccccugugggc aggacccaac cagaa cucug ugccugu cccuuagugc
 
>GPX6_SECIS_bosTau Bos taurus (cow) COVE score: 23.33 
cucacuucac augagga aaagggcaucucc  augau  gguggguccc  aa  agccucucuggguc ggaccccacc agag ccu uccuugg ugccuguccc
 
>GPX6_SECIS_eriEur Erinaceus europaeus (hedgehog) COVE score: 24.33
ccuggccuca uaugaaa ggagggcaucucc  augau  gguggguucc  ca  agccccugcgguc ggacccuacc agag ccu uccuugg ugucuguccu

>GPX6_SECIS_sorAra Sorex araneus (shrew) frag COVE score: 22.95
uuucauauga aaggagg gggcaucucc  augau  ggugggucuc  aa  agccccucuggguc ggacccuacc agag cccugcugg guguccu gucccuuugu

>GPX6_SECIS_dasNov Dasypus novemcinctus (armadillo) COVE score: 19.82 
cucaccuuac augaagg gagggacauaucc  augau  gguggguccu  aa  agcccuucuggguc agacccuacc agaa ccu ucucug gugccugucc

>GPX6_SECIS_loxAfr Loxodonta africana (elephant) COVE score: 19.28 
cucaccucac augaaug gaggggcauaucc  augau  gguggguccc  aa  agccucucuagguc ggacccuauc agaa ucuucccugg ugccugu cccuuagugc
 
>GPX6_SECIS_echTel Echinops telfairi (tenrec) frag COVE score: 31.33 
ugugucccag aaugggg agguggcaucauc  augac  agcggggucu  ga  aagccccuccuggau ggaccccgcc cgaa ccu ucccug gugccugucc

GPX1: 17 SECIS insertion sequences

>GPX1_homSap Homo sapiens (human) COVE score: 20.45
UGCUGUCUCG GGGGGGU UUUCAUCU AUGAG GGUGUUUCCUCU AA ACCUACGA GGGAGGAACACC UGAU CUUACAGAAA AUACCAC CUCGAGAUGG

>GPX1_panTro Pan troglodytes (chimp) COVE score: 20.45 
gugcugcucu guggggg gguuuucaucu  augag  gguguuuccucu  aa  accuacga gggaggaacacc ugau cuuacagaaa auacccc cucgagaugg

>GPX1_macMul Macaca mulatta (rhesus) COVE score: 26.45 
gcagcgcugc ucucugg gggguuuucaucu  augag  gguguuuccucu  aa  accuaca aggaggaacacc ugau aau acagaa aauacccccu

>GPX1_otoGar Otolemur garnettii (bushbaby) COVE score: 25.30 
auagcgcugc ucucugg ggggguuucauuc  augau  aguguuaccucu  aa  acuugcau gggggaacacc ugau gcc ccagaaa auccccugag

>GPX1_tupBel Tupaia belangeri (tree_shrew) COVE score: 21.14
cagcgcugcg ucugggg gguuucaucc  augac  ggugucuccucu  aa  accccga aggaggaacgcc ugau guccggaaaa cccccca ggugggcgcc

>GPX1_musMus Mus musculus (mouse) COVE score: 29.78 
ggcugcacuc ugggggg cgguucuucc  augau  gguguuuccucu  aa  auuugca cggagaaacacc ugau uuccaggaa aaucccc ucagaugggc

>GPX1_ratNor Rattus norvegicus (rat) COVE score: 25.72
ggcugcccuc cgggggg agguuuuucc  augac  gguguuuccucu  aa  auuuaca uggagaaacacc ugau uuccagaaa aaucccc ucagaugggc

>GPX1_cavPor Cavia porcellus (guinea_pig) COVE score: 15.34 
uugcccagau gcucucc ugaggguucuucc  augaa  gguguuuccucuc  aa  ccugua uagaggaacauc cgau uccca ggaauu ucccagagag

>GPX1_oryCun Oryctolagus cuniculus (rabbit) COVE score: 24.38 
guggccugcu gcucucu ggggguuucaucc  augag  ggcguucccccg  aa  aacaaa uggaggaacgcc ugau gucc gggaaac ccccaggugg

>GPX1_eriEur Erinaceus europaeus (hedgehog) COVE score: 19.31 
auagccgcga gccgcug ggcaucucaucc  augac  ggcgccgccuuc  aa  accugcgagcag gaaggagcgcc cgau agc cgcgaga gcccccagcg

>GPX1_canFam Canis familiaris (dog) COVE score: 22.40 
ucacagcgcu gcccucu ggggauuucaucc  augau  gguguuuccuug  aa  aucugca uggaggaacgcc ugau uucca ggaaagu ccccugagcu

>GPX1_felCat Felis catus (cat) COVE score: 27.75 
cacagcacug cccucug gggauuucaucc  augau  ggcguuuccucg  aa  auuugca uagaggaacgcc ugau uuc cagaag aauccccuga

>GPX1_equCab Equus caballus (horse) COVE score: 27.08
ucacagugcu uuucucu ggggauuucaucc  augau  ggcguuuccucu  aa  acaugc augaggaacgcc ugau guu aaggaga aucccccgag

>GPX1_bosTau Bos taurus (cow) COVE score: 29.83 
agucagcgcu gcucucc agggauuuugccc  augaa  gguguucccucu  aa  accuacg uggaggaaugcc ugau gucca ggaaaau ccccugaggu

>GPX1_dasNov Dasypus novemcinctus (armadillo) COVE score: 28.92
uguccuccac acggggu uuucaucc  augac  gguguuuccucu  aa  accugca gggaggaacacc ugaa guccggcaaa aucccc cgagaugggu

>GPX1_loxAfr Loxodonta africana (elephant) COVE score: 25.48 
acuaggcggc uuuccgu ggggguuucauua  augag  gguguuuucucu  aa  accugaa uggaggaacacc ugau guc ugggaa gauacccccc

>GPX1_monDom Monodelphis domestica (opossum) COVE score: 26.38 
gugcuaaggg uccguga ggguuuuaucu  augau  gguguuguuuu  aa  accauuaagga gaaagaacacu ugau aaugc uuguaa aaucccauga

>GPX1_ornAna Ornithorhynchus anatinus (platypus) COVE score: 25.55
ugcuauuugc ccauggg ggagaucucauuu  augau  ggugcuccuucu  aa  accuuua cggaagagcacu ugau gga uccugag gaacuccaug

GPX2: 17 SECIS insertion sequences

>GPX2_homSap Homo sapiens (human) COVE score: 29.54 
AGACUUGGGU AAGCUCU GGGCCUUCACAGA  AUGAU  GGCACCUUCCU  AA  ACCCUCA UGGGUGGUGUC UGAG AGGCGUGA AGGGCCU GGAGCCACUC

>GPX2_panTro Pan troglodytes (chimp) COVE score: 29.54 
agacuugggu aagcucu gggccuucacaga  augau  ggcaccuuccu  aa  acccuca ugggugguguc ugag aggcguga agggccu ggagccacuc

>GPX2_macMul Macaca mulatta (rhesus) COVE score: 29.54
agacuugggu aagcucu gggccuucacaga  augau  ggcaccuuccu  aa  acccuca ugggugguguc ugag aggcguga agggccu gaagccaccc

>GPX2_otoGar Otolemur garnettii (bushbaby) COVE score: 27.86  
agacuugggu gggcucu gggccuucacaga  augau  ggcaccauccu  aa  acgccuc ugggugguguc ugag aagagcg gaaggcc uggagccacc

>GPX2_tupBel Tupaia belangeri (tree_shrew) COVE score: 28.69 
agacuuaggu gggcucu gagccuucacaga  augau  agcaccuuccu  aa  acccccc cgggagguguc ugag aagugug acaggcc cggagccagc

>GPX2_musMus Mus musculus (mouse) COVE score: 33.65 
agucuggggu agguucu gggccuucacaga  augau  ggcaucuuccu  aa  acccuuc ugggagauguc ugag aaguugug aaggguc cagagccagu

>GPX2_ratNor Rattus norvegicus (rat) COVE score: 30.80 
cugggguagg ugcuagg ccuucuucacaga  augau  ggcaucuuccu  aa  acccuuc ugggggauguc ugag acg uugugaa gggcccagag

>GPX2_cavPor Cavia porcellus (guinea_pig) COVE score: 20.08  
cuaggacagg uggcucu gggccuucacaga  augac  ggcaccguccu  aa  acgcua ugggugguauc ugag aagugug aauggcug gagccagccu

>GPX2_sorAra Sorex araneus (shrew) COVE score: 29.00 
aagcuggggc aaacucu gggccuucgcaga  augau  ggcaccucccu  aa  auccau ggggugguguc ugag gcgugcg agggccu ggaaacagcc

>GPX2_eriEur Erinaceus europaeus (hedgehog) COVE score: 25.06 
aaagcugggu gguuucu ggaccuucacaga  augau  agcaccuuccu  aa  accuaua gggaugguguc ugag aaaugu gaagggc cugaaguaaa

>GPX2_canFam Canis familiaris (dog) COVE score: 22.57  
aagacuuggg uggcucu gggccuucacaga  augau  ggcaccuuccu  aa  auagua ugggcgguguc ugag aagugug aagggcu cagagccagc

>GPX2_felCat Felis catus (cat) COVE score: 22.45 
aagacuuggg uggaucu gggccuucacaga  augau  ggcaucuuccu  aa  acugua ugggcgguguc ugag gaguguga agggcu cggagccagc

>GPX2_equCab Equus caballus (horse) COVE score: 28.45
agacuucggu aggcucu gggccuucacaga  augau  ggcaccuuccu  aa  accugua uggacgguguc ugaa aagcgug aagggcc ccgagucagc

>GPX2_bosTau Bos taurus (cow) COVE score: 26.14
agacaugggu gggcucu gugucuucacaga  augau  ggcaccuuccu  aa  aucugua ugggcgguguu ugag aagagu gaaggcc uggagccagc

>GPX2_loxAfr Loxodonta africana (elephant) COVE score: 27.08  
agacuugggu aggcucu ggaccuucgcaga  augau  ggcaccuuccu  aa  acucag ugggugguguc ugag aaaugug aagggccu agggccagcc

>GPX2_echTel Echinops telfairi (tenrec) COVE score: 29.95 
agugcuggug uggcucu gggccuucacaga  augau  ggcaccuuccu  aa  acccuc cggaagguguc ugag aaaugug aagggcc uggggccggc

>GPX2_monDom Monodelphis domestica (opossum) COVE score: 26.38 
gugcuaaggg uccguga ggguuuuaucu  augau  gguguuguuuu  aa  accauuaagga gaaagaacacu ugau aaugc uuguaa aaucccauga

>GPX2_ornAna Ornithorhynchus anatinus (platypus) COVE score: 27.55
gggcucgagc agccucc agaccuucacaga  augac  ggugucuccuu  aa  acccuaac cgggaggcacc cgag agccggu gaagggc cuggugacug

GPX4: 14 SECIS sequences

>GPX4_SECIS_homSap Homo sapiens (human) chr19:1,057,512-1,057,793 COVE score: 29.19
CCACGCCCUU GGAGCCU UCCACCGGCACUC  AUGAC  GGCCUGCCUG  CA  AACCUGCUGGU GGGGCAGACC CGAA AAUCC AGCGUGC ACCCCGCCGG

>GPX4_SECIS_panTro Pan troglodytes (chimp) COVE score: 29.19
CCACGCCCUC GGAGCCU UCCACCAGCACCC  AUGAc  ggccugccug  ca  aaccugcuggu ggggcagacc cgaa aaucc agcgugc acccugccgg

>GPX4_SECIS_macMul Macaca mulatta (rhesus) COVE score: 33.09
CCACGCCCUC GGAGCCU UCCACCGGCACCC  AUGAc  ggccugcuugc  aa  accagcuccuggu gaggcagacc cgaa aaucc agcgugc accccgcugg

>GPX4_SECIS_tupBel Tupaia belangeri (tree_shrew) COVE score: 31.05 
CCGCGCCCCG GAACCUU CCACCCGGCACCU  UUGAc  ggucugccuau  aa  accugccacug gugaggcagacc cgag aaccu ggcgugc acccugccgg

>GPX4_SECIS_musMus Mus musculus (mouse) COVE score: 35.62 
UGACCCCUGG AGCCUUC CACCCCGGCACUC  AUGAa  ggucugccug  aa  aaccagccugcuggu ggggcagucc ugag gaccu ggcgugc acccugccgg

>GPX4_SECIS_ratNor Rattus norvegicus (rat) COVE score: 34.33
UGACCCCUGG AGCCUUC CACCCCGGCACUC  AUGAc  ggucugccug  aa  aaccagcccgcuggu ggggcagucc cgag gaccu ggcgugc accccgccgg

>GPX4_SECIS_cavPor Cavia porcellus (guinea_pig) COVE score: 34.46 
UGGCCGCCCC GAGUCUC CUACCCGGGUGCC  AUGAc  ggccugccug  ca  aaccagcgug cugguggggc agac ccg aggaugc gugcacugcc

>GPX4_SECIS_canFam Canis familiaris (dog) COVE score: 31.12 
AUGCCCCUCG GAGCCUU CCACCCGGCACCC  AUGAc  agucugucuaa  aa  accagcccgcugg uggggcagacc cgag aaccc ggcgugc acccugccgg

>GPX4_SECIS_sorAra Sorex araneus (shrew) COVE score: 36.85 
CUGCCCCUCG GAGCCUU CCACCCGGCGCCC  AUGAc  ggucugccugc  aa  accagccc gcugguggggc agac ccgagaaccc ggcgugc accuugccgg

>GPX4_SECIS_bosTau Bos taurus (cow) COVE score: ?
tgttccgagaAGCCTTCCCCCGGtgCctATGACGGTCTGtCTGAAAACCAGCCCCTGGTGGGGCAGaCCtGAGaACCTGGCGTGCACtccttccgg

>GPX4_SECIS_echTel Echinops telfairi (tenrec) COVE score: 40.88
UUGAUCCCCA GAGUCUU CCACCCGGCACCA  AUGAc  ggucugccuuc  aa  accaggccucugg ugaggcagacc cgau gaccc ggcgugc acucagccgg

>GPX4_SECIS_monDom Monodelphis domestica (opossum) COVE score: 40.88 
UUGAUCCCCA GAGUCUU CCACCCGGCACCA  AUGAC  GGUCUGCCUUC  AA  ACCAGGCCUCUGG UGAGGCAGACC CGAU GACCC GGCGUGC ACCUCAGCCG

>GPX4_SECIS_ornAna Ornithorhynchus anatinus (platypus) COVE score: 33.89
ACCUCCGCUU UCCCGGG ACGCUCUGCCUCC  AUGAc  ggccggccuu  ca  agccaaaaccaguugg uggggccggcc ugaa caa accggca cgggucccgg

>GPX4_SECIS_anoCar Anolis carolinensis (lizard) COVE score: 38.77
UGCUCCCCGU GGGCCCC CUCCUCCAGCACC  AUGAc  ggccugccuug  aa  gccagcuugcugg ugaggcagacc cgaa gauuc ggcgugc acugcuggag

>GPX4_SECIS_galGal Gallus gallus (chicken) COVE score: 38.77
UGCUCCCCGU GGGCCCC CUCCUCCAGCACC  AUGAc  ggccugccuug  aa  gccagcuugcugg ugaggcagacc cgaa gauuc ggcgugc acugcuggag

SEPW: 13 SECIS sequences

>SEPW_SECIS_homSap Homo sapiens (human) COVE score: 36.01
GACCCAGCCC CUCUCAG CAGACGCUUC AUGAU AGGAAGGACUG AA AAGUCUUGUGGACACC UGGUCUUUCCC UGAU GUU CUCGUGG CUGCUGUUGG

>SEPW_SECIS_panTro Pan troglodytes (chimp) COVE score: 36.01
GACCCAGCCC CUCUCAG CAGACGCUUC AUGAU AGGAAGGACUG AA AAGUCUUGUGGACACC UGGUCUUUCCC UGAU GUU CUuGUGG CUGCUGUUGG

>SEPW_SECIS_ponPyg Pongo pygmaeus (orang_sumatran)C OVE score: 30.55
GACCCAGCCC CUCUCAG CAGACGCUUC AUGAU AGGAAGGACUG AA AAAUCUUGUGGACACC UGGUCUUUCCC UGAU GUU CUCGUGG CUGCUGUUGG

>SEPW_SECIS_macMul Macaca mulatta (rhesus) COVE score: 33.55
GACCCAGCCC CUCUCAG CAGACGCUUC AUGAU AGGAAGGACUG AA AAGUCUUGUGGACgCC UGGUCUUUCCC UGAU GUU CUCGUGG CUGCUGUUGG

>SEPW_SECIS_musMus Mus musculus (mouse) COVE score: 34.04
UUGGCCCAGC CCCUCGU GGCAGACGCUUC AUGAU GGGAAGAACUG AA AUGUCUCGUGGACGC CUGGUCUUUCC CUGAU GUCCCU GCGACUG CCACGUAGGG

>SEPW_SECIS_ ratNor Rattus norvegicus (rat) COVE score: 26.55
UGGCCGGCCU UUCUUGG CAGCCGCUUC AUGAC AGGAAGGACUG AA AUGUCUCAAAGACCUG UGGUCUUUCUU CGAU GUU CCUGCGG CCACCAAGUC

>SEPW_SECIS_oryCun Oryctolagus cuniculus (rabbit) no SECIS element found
AGTAACCTTGACCCAGCCCCTTTCATGCCTCAGCCTCGTCTCCATAGGCTAAGACTGGAGAAATGAGTCCCCTGAAGAACTGAAACTGGGGGTAGAGGGTTGGTGTTTTAAGATGTGGATGAGCTGGTCTTTAC

>SEPW_SECIS_canFam Canis familiaris (dog) COVE score: 34.04
UUGGCCCAGC CCCUCGU GGCAGACGCUUC AUGAU GGGAAGAACU GA AAUGUCUCGUGGACGCC UGGUCUUUCC CUGAU GUCCCU GCGACUG CCACGUAGGG

>SEPW_SECIS_susScr Sus scrofa (pig) no SECIS element found
AGTAACCTTGACCCAGCCCCTTTCATGCCTCAGCCTCGTCTCCATAGGCTAAGACTGGAGAAGTCTTGTGGACGCCTGGTCTTTCCCTGATGTTCTCGTGGCTGCTGTTGGGGGCAGAGATGGATGAGCTGGTCTTTAC

>SEPW_SECIS_borAnc Boreoeuthere ancestralis (ancestral) COVE score: 33.54
GGCCCAGCCC CUCUCAG CAGAUGCUUC AUGAC AGGAAGGACUG AA AUGUCUUGUGGACGCC UGGUCUUUCCC UGAU GUU CUUGUGG CUGCUGGUUG

>SEPW_SECIS_dasNov Dasypus novemcinctus (armadillo) COVE score: 28.71
CCCAGCUGCC CUUGGCA GACGCUUC AUGAG GGGAAGGACCU AA AUGCGUCGUGGAUGCC UGGUCUUUCCC UGAU GCUCCUUCAC CUGCCAG AUGGGGCAGA

>SEPW_SECIS_loxAfr Loxodonta africana (elephant) no SECIS element found
GGGACCTTGGCCCAGCCCCTTTCAGCAGACACTTCATGACAGGAGGACTGAAATGTCTCCCAGACGCCTGGCTCTTTCCCTGAATCTGTCGGCTGCAGGACAGGGCAGCGGTTGACTCTCTCGTTTTTTGCAT

>SEPW_SECIS_echTel Echinops telfairi (tenrec) no SECIS element found
AGGCCAGAGACCTTGGCCCAGTCCCTCCATGACAGGCAGAACTGAAATGTCCTCTGGACAAGTGGTCTTTTCCAGAAACCCCAGGGCTGCTGGGCCGGAGCCGAGGCTGACAACCCTGGTCTTTGCCT

DIO1: 29 SECIS sequences

>DIO1_SECIS_homSap Homo sapiens (human) COVE score: 29
ttttaactctgtgtctttacatatttgtttatgatggccacagcctaaagtacacacggctgtgacttgattcaaaagaaaatgttataag

>DIO1_SECIS_ponPyg Pongo pygmaeus (orang_sumatran) COVE score: 29
ttttaactctgtgtctttacatatttgtttatgatggccacagcctaaagtacacacggctgtgacttgattcaaaagaaaatgttataag

>DIO1_SECIS_macMul Macaca mulatta (rhesus) COVE score: 29
tttcaactctgtgtctttacatatttgtttatgatggccacagcctaaagtacacacggctgtgacttgattcaaaagaaaatgttataag

>DIO1_SECIS_cavPor Cavia porcellus (guinea_pig) COVE score: 24
tgttaactctgcttcttttcatatttgttcatgacggtcacagtctaaagtacacacagctgtgacctgatttgaaagaaaatgttttaag

>DIO1_SECIS_canFam Canis familiaris (dog) COVE score: -
ttttaactctgcttcttttcatgtttgtctatgacggccacagcctaaagcacacacagctgtgacttgatttgaaagaaaatgttttaag

>DIO1_SECIS_felCat Felis catus (cat) COVE score:  -
ttttaactctgcttcttttcacgtttgtctatgacggccacagtctaaagtgcacacagctgtgacttgacttgaacgaaaatgttttaag

>DIO1_SECIS_bosTau Bos taurus (cow) COVE score: 28
ttttaactctgcctcttttcatatttgttcatgacggccacagcctaaagtacacacggctgtgacttgatttgaaagaaaatgttttaag

>DIO1_SECIS_sorAra Sorex araneus (shrew) COVE score: 24
cggaaactcagcttctcttcatatttgtttatgacagccccagctgaaagtacacacagctgtggcttgattggaaagaaaatgttttaag

>DIO1_SECIS_eriEur Erinaceus europaeus (hedgehog) COVE score: 26
tttaactctgctttcttctcatatttgcttatgatggtcacagcttaaagtatacacagctgtgacttgattggaaagaaaatattttaag

>DIO1_SECIS_borAnc Boreoeuthere ancestralis (ancestral) COVE score: 29
ttttaactctgcttcttttcatatttgttcatgatggccacagcctaaagtacacacggctgtgacttgatttgaaagaaaatgttttaag

>DIO1_SECIS_dasNov Dasypus novemcinctus (armadillo) COVE score: -
ttttaactctgcttcttttcatatttgtttatgatggccacagtttaaagtacatacagctgtgacttgatatgaaaaagaaatattttaag

>DIO1_SECIS_loxAfr Loxodonta africana (elephant) COVE score: - 
ttttaactctgcttcttttttcatgtatttatgatgggccacagcctaaagtgcacaacagctgtgacttgatttgaaaaacatctttaag

>DIO1_SECIS_monDom Monodelphis domestica (opossum) COVE score: -
tttccatcctgcttctacaaatatttatttatgacaatcacagcctaaagctcagggcagctgggattcgacgggagaaaaagtttgtaag

>DIO1_SECIS_ornAna Ornithorhynchus anatinus (platypus) COVE score: 24
ccccggatccggttccgtgaatattggtttatgagggtcacagtgtaaagcgcatgcagctgtgacttgatctgagaaaatatttctgcggc
 
 
>DIO2_SECIS_homSap Homo sapiens (human) 5,630 bp utr COVE score: 30
cagagatgtgcagagttgaccagtgtgcggatgataactactgacgaaagagtcatcgactcagttagtggttggatgtagtcacattagtttgcctctc

>DIO2_SECIS_macMul Macaca mulatta (rhesus) COVE score: 30
cagagatgtgcagagttgaccagtgtgcggatgataactactgacgaaagagtcatcgactcagttagtggttggatgtagtcacattagtttgcctctc

>DIO2_SECIS_musMus Mus musculus (mouse) COVE score: 29
cggagatgttcagagctcactggtgtgcgaatgataactactgacgaaagagctgtctgctcagtctgtggttggatgtagtcacacgagtctgcctttctgca

>DIO2_SECIS_ratNor Rattus norvegicus (rat) COVE score: 28
ccgagatgttcggagctcactggtgtgcgaatgataactactgacgaaagagtcatctgctcagtctgtggttggatgtagtcacacgagtctgcctctccatc

>DIO2_SECIS_canFam Canis familiaris (dog) COVE score: 27
ctgggatgtgcagaggtgaccagtgtgcgaatgataactactgatgaaagagtcactgactcagttagtggttggatacagtcacattagttttcctct 

>DIO2_SECIS_borAnc Boreoeuthere ancestralis (ancestral) COVE score: 30
ctgggatgtgcagaggtgaccagtgtgcaaatgataactactgatgaaagagtcattgactcagttagtggttggatgtagtcacattagtttgcctctc

>DIO2_SECIS_dasNov Dasypus novemcinctus (armadillo) iodothyronine deiodinase type II
ctgggaagttcagaggctaccagtgtgccaatgataactactgacgaaagaggcatcgactcagttagtggttggatgtagccacattagtttgcctctc
 
 
>DIO3_SECIS_homSap Homo sapiens (human) COVE score: 31
ttgggtgcacaggagccccactgctgatgacgaactatctctaactggtcttgaccacgagctagttctgaattgcaggggcctcaaagcagca

>DIO3_SECIS_macMul Macaca mulatta (rhesus) COVE score: 30 
ttgggtgcacaggagccccactgctgatgacgaactgtctctaactggtcttgaccacgagctagttctgaattgcaggggcctcaaaacagca

>DIO3_SECIS_musMus Mus musculus (mouse) COVE score: 26  
ttgggtgcgctggagccctggctgctgatgacgaaccgcctctaactgggcttgaccacgggtcggctctgaattgcagagaggctcgaaacagc

>DIO3_SECIS_ratNor Rattus norvegicus (rat) COVE score: 26 
ttgggtgcgctggagccctggctgctgatgacgaaccgcctctaactgggcttgaccacgggtcggctctgaattgcagagaggctcgaaacagc

>DIO3_SECIS_canFam Canis familiaris (dog) COVE score: 26
ttgggtgctggcgagccccactgctgatgacgagccgcctctaactggtcttgaccacgagctggttctgagttgcaggggggcttgcagcggc

>DIO3_SECIS_bosTau Bos taurus (cow) COVE score: 30
ttgggtgctcacgagccccactgctgatgaagagctgtctctaactggcctcgaccacgagctggttctgatttgcaggaggctcgcagcagc

>DIO3_SECIS_borAnc Boreoeuthere ancestralis (ancestral) COVE score: 27
ttgggtgctcaggagccccactgctgatgacgaactgtctctaactggtcttgaccacgagctggttctgaattgcagggggctcgcagcagca

>DIO3_SECIS_loxAfr Loxodonta africana (elephant) COVE score: 22
ttcggtgcgctagagccccactgctgatgacgaactgtctctaactggtcttgaccacgagctgattccgaattgcagggaactcgcagcagc

>DIO3_SECIS_echTel Echinops telfairi (tenrec) COVE score: -
ttcggtgctctgcagccccactgctgatgacgaactgcctctcactggtcttgaccacgagctgcttctgaaatgcaggggactcgcagccgca