Adding New Tracks to a browser installation
Adding a custom track
See Also
For a generic discussion of how to load any type of track into your local browser mirror, refer to the document in the source tree src/product/README.trackDb and the discussion of how the trackDb entries function in src/hg/makeDb/trackDb/README
Gotchas
Your .bed file better not have any browser or track lines if you want the loader to work
You need to hop all over the source tree like a frog on a griddle to get things done...
The docs are a little misleading perhaps. I seemed to need a .hg.conf file in my home directory - setting env options didn't seem to help a bit for commandline building access.
Instructions
From the readme on addTrack.txt
I. Introduction.
This describes how to add a track to the UCSC genome browser. The two major steps in adding a track are creating a table containing the track information, and putting a description of the track in trackDd. The browser has one mysql database for each version of each genome that it displays. Both the track table and the track description live in this database. The current human genome database is hg13, while the current mouse database is mm2.
II. MySQL Preliminaries.
Before you get started it is good to look at these databases a little, and make sure that you have update access to them. You could do this directly with the 'mysql' command, but let's do it instead with the 'hgsql' command, which will keep you from having to type your user name and password all the time.
Assuming you've got the browser source already installed in ~/kent/src do the following to create hgsql cd ~/kent/src/lib make This make almost always goes smoothly on Linux. You may need to remove the '-ggdb' flag in the makefile on other systems, and possibly set up a MACHTYPE environment variable, and then mkdir $MACHTYPE on some systems. Next cd ../hg/lib make The main problem that can happen with this make is if the mysql libraries and include files are not found. See kent/src/README for details. The next step is cd ../hgsql make rehash The hgsql program is just a thin wrapper around mysql. It looks for the password and username in the file ~/.hg.conf. Here's the necessary parts of .hg.conf:
- db.host is the name of the MySQL host to connect to
db.host=localhost
- db.user is the username is use when connecting to the host
db.user=mysqlUserName
- this is the password to use with the above hostname
db.password=mysqlPassword
This .hg.conf is similar to the cgi-bin/hg.conf file that the browser uses, but it need not contain everything that file does. Also it's advisable to have a read-only user/password in cgi-bin/hg.conf while you'll want a read-write user/password in ~/.hg.conf. Setting this up can involve doing some 'grants' in mysql. See the documentation at www.mysql.com for how do set up various users.
Assuming your mysql and .hg.conf are set up, and that you already have a mirror site going then the command hgsql database (where database is something like hg13 or mm2) should bring you to the mysql prompt. Do mysql> show tables; and you should see a large list of tables. When you've finished adding a track, the track(s) for your tables will be among them. Also try doing: mysql> describe trackDb; This will list the fields of the trackDb table, which has a row for each track. Then do mysql> select tableName,shortLabel,type from trackDb; This will show you some of the key fields from this table. You won't be updating this table directly, but it can be handy to look at it sometimes for debugging purposes. Some other useful mysql commands are
mysql> select count(*) from sex;
This will count up all the items in the sex table. You thought there were only two? This table reflects the diversity of the sex fields in genbank. Try mysql> select * from sex; to see the full diversity. Well, enough of that non-normalized nightmare. To get out do:
mysql> quit
III. Loading the main track table.
The UCSC Genome Browser Database is usually loaded from a text file of some sort. The most popular types of text files are .psl files for blat alignments, .bed files for a wide variety of data, and GTF files for gene predictions. See http://genome.ucsc.edu/goldenPath/help/customTrack.html for further information on these formats. For now we'll assume you have a file in one of these formats that you want to add to the browser.
A) Creating the loader programs cd ~/kent/src/hg/makeDb cd hgLoadPsl make cd ../hgLoadBed make cd ../ldHgGene make cd ../hgPepPred
The makeDb directory also contains loaders for a number of more specialized tables including hgLoadOut for RepeatMasker data. There is also a .doc file describing in detail how we created each database in the files named things like makeHg13.doc and makeMm2.doc.
B) Loading a bed file
Loading a bed file is the most straightforward. First decide on
the name you want to call the table. Then do
hgLoadBed database tableName file.bed
type hgLoadBed with no arguments for further information.
Database will be something like hg13 or mm2.
C) Loading a psl file
Loading a psl file is also easy. Make sure that the psl file
is sorted by chromosome (tName) and start position (tStart). Use
kent/src/hg/pslSort or just plain Unix sort for this if necessary.
If the number of alignments is somewhat modest (say less than
500,000) then do
hgLoadPsl database -table=tableName file.psl -tNameIx
This will load everything into one big table. For huge numbers of
alignments the browser will be faster if you first split up the
data into one file for each chromosome. Name these files
chr1_tableName.psl chr2_tableName.psl and so forth. Then do
hgLoadPsl database chr*_tableName.psl
This will end up making a separate table for each chromosome.
Unfortunately it is still a bit complicated to make the details
pages for a psl format track to include the alignments themselves.
Please contact us at UCSC if this is a priority for you and we
will try to make it easier.
D) Loading a GTF (or GFF) file
Generally GTF is a much more tightly defined standard than GFF, so
GTF files are more likely to work without tweaking. However most
reasonable GFFs will work as well. To load do
ldHgGene database tableName file(s).gtf
This will make a gene-prediction type table. You often will want
to create an associated predicted peptide table as well. To do this
do
hgPepPred database generic tableNamePep file(s).fa
The first word after the '>' in the fasta files should use the same
symbol as the 'group' in GFF files or 'transcript_id' in GTF files.
IV. Updating trackDb
Your data will not display in the browser until you load it into trackDb. To do this first cd ~/kent/src/hg/makeDb/trackDb and look at the file trackDb.ra, and read the README file. Then decide whether your new track should be global, organism specific, or assembly specific, and edit the corresponding trackDb.ra file. Generally it's good to find an existing track as similar as possible to the track you want to add, copy and paste it, and modify the copy. Then put any explanitory text you want on the track in trackName.html in the appropriate directory. After this do a make alpha to update the trackDb table, or a
make
to update trackDb_user (where user is you Unix username). If multiple engineers are working on the project you can set up cgi-bin-user directories with hg.conf files that will tell the browser to use trackDb_user instead of trackDb to avoid conflicts with other engineers code.
After the 'make alpha' the browser should show your track. Congratulations if you've made it this far. See also http://www.soe.ucsc.edu/~sugnet/doc/trackHowto/browserTalk.pdf for Charles Sugnet's description of how to add a track including some code customization.
-Jim Kent Feb 14, 2003.
A BED track description for a custom SNP track
The .bed file has lines like this:
chr22 49512530 49512531 rs8137951 0 + 49512530 49512531 255,0,0 chr22 49518559 49518560 rs756638 0 + 49518559 49518560 255,0,0 chr22 49522492 49522493 rs3810648 0 + 49522492 49522493 255,0,0 chr22 49524956 49524957 rs2285395 0 + 49524956 49524957 255,0,0 chrX 100017093 100017094 rs5921682 0 + 100017093 100017094 255,0,0 chrX 100019802 100019803 rs5967204 0 - 100019802 100019803 255,0,0
Load the .bed file into a custom track called Ill550v3 Note the .bed file must have NO browser or track lines - otherwise hgLoadBed will barf
/var/www/cgi-bin/loader/hgLoadBed -noBin -tab hg18 Ill550v3 /home/rerla/public_html/illHH550v3.BED
Adjust your trackDb.ra file
I'm working (eg) on meme in
/home/rerla/hg18/kent/src/hg/makeDb/trackDb/human/hg18
I add these settings - they seem to work the way I want, allowing the name field from the .bed file to appear in full view:
track Ill550v3 priority 20.1 shortLabel Illum550kv3 longLabel Illumina 550k v3 snps visibility pack type bed 9 . color 255,0,0 group varRep thickDrawItem on
Recompile the trackDb
make alpha DBS=hg18
