DHFR dihydrofolate

Vertebrate reference sequences

>DHFR_homSap Homo sapiens (human) AC130896 tightly syntentic -+ MSH3 chr5
0 MVGSLNCIVAVSQNMGIGKNGDLPWPPLR 2
1 NEFRYFQRMTTTSSVE 1
2 GKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELK 2
1 EPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYK 0
0 EAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPE 2
1 YPGVLSDVQEEKGIKYKFEVYEKND* 0

>DHFR_panTro Pan troglodytes (chimp) XM_001138522
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELASKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_gorGor Gorilla gorilla (gorilla) CU104654
MVGSLNCIVAVSQNMGIGKNGVLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_ponAbe Pongo abelii (orangutan) XM_002815704 syntenic 
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_nomLeu Nomascus leucogenys (gibbon) XM_003261693
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNYPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEFPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_macMul Macaca mulatta (rhesus) XM_001110551 syntenic
MVSSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_papAnu Papio anubis (baboon) FC175564
MARSLNCIVAVSQNMGIGKNGDLPWPPLRSEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGNSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_calJac Callithrix jacchus (marmoset) XM_002745986 tightly syntentic -+ MSH3 4 recent pseudogenes
MVQLLNCIVAVSQNMGIGKNGELPWPPLRNEFRYFQRMTTTSSVEDKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGALLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPQYPGVPSDDQEEKGIKYKFEVYEKND 

>DHFR_saiBol Saimiri boliviensis (squirrel_monkey) AGCE01006205/AGCE01053749 numerous pseudogenes syntentic MSH3
MVQLLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSKELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIIGGSSVYKEAMDHPGHLKLFVTRIMQDFESDTFFPEIDlkkykllseYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_tarSyr Tarsius syrichta (tarsier) ABRT010238682/ABRT010238686/ABRT010468683
MVRPLYCIVAVSQNMGIGKDGTLPWPPLRNEFRYFQRMTTMSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKVPPPGAHFLAKSLDDALKLIEQPELANKVDKVWIVGGNSVYREAMSQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_otogar Otolemur garnettii (bushbaby) AAQR03040271/AAQR03040268
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFNYFQRMTKTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESPQGAHFLAKSLDDALKLIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYKFEVYEKND

>DHF1_micMur
MVRTLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPE YPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_tupBel Tupaia belangeri (treeshrew) AAPY01231248/AAPY01231253/AAPY01729648/SRA|SRR005484.246484.2
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNIVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANQVDMVWIVGGSSVYREAMNQPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPE
 
>DHFRL1_homSap Homo sapiens (human) NM_001195643 synteny +ARL13B/-DHFRL1/+NSUN3 chr3
MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGVLSDVQEGKHIKYKFEVCEKDD

>DHFRL1_panTro Pan troglodytes (chimp) ti|1986883368 syntenic short c-terminal
MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLNDRINLVLSRELKEPPQGAHFFARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGVLSDVQEGKHIKYKFEVYEND

>DHFRL1_gorGor Gorilla gorilla (gorilla) ti|2051780408/ti|2033585870 10 bp deletion so frameshift synteny unknown but 97% identical homSap
MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAISLDDALKLTERPKLANKVDMIWIVGGSSVYKEAMNh lKLFVTRIMQDFASDTFFSEIDLEKYKLLPEYPGVLSDVQEgKHIKYKFEAYEKND
 
>DHFRL1_ponAbe Pongo abelii (orangutan) genome/blat syntenic 
MVRLLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL1_nomLeu Nomascus leucogenys (gibbon) ADFV01005477 syntenic 
MVRLLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLEDRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNRSGHLKLFVTRIMQDFESDMFFSEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL1_macMul Macaca mulatta (rhesus) genome/blat syntenic
MVRSLNCIVAVSQNMGIRKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL1_papHam Papio hamadryas (baboon) ti|1986883368 synteny unknown 
MVHSLNCIVAVSQNMGIGKNGDLPWPPLRNEFGYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLARSLDDALKLTEQPELANKVDMIWIVGGSSVYKEAMNHPGHLKLFLTRIMQDFESDTFLPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKN

>DHFRL1_calJac Callithrix jacchus (marmoset) genome/blat frameshift and stop codon but syntenic
mvrllCIVAVSQNMGIGKKGDLPWPPLRNEFRYFQRMTTTSSIEGKQNLVIMGRKTWFSIPEKNRPLKDRINFVLSRELQEPPQGAHFLARSLDDALNLTEQPELANKVDMIWIVGGSSVYKEAMNRPGHLKLFVTRIMQDFESDTFFPEIDLEK*KLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL1_otogar Otolemur garnettii (bushbaby) AAQR03025778 synteny unknown
MVHRLNCIIAVSQNRGIGKNGDLPWPPLRNEFNYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKESSQGAHFLAKSLDDALKHIEEPELANKVDMVWIVGGSSVYKEAMNQPGHVKLFVTRIMQDFECDTFFPEIDLKKYKLLSEYPGVLSDVQEEKGIKYRFEVYEKND

>DHFRL2_micMur Microcebus murinus (lemur) ABDC01288917 synteny unknown 
MVRTLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQKMTTTSSVEGKQNLVIMGRKTWFSIPEKSRPLKDRINVVLSRELKEPPQGAHFVAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAMNQPGHLKLFVTRIMQDFESDTFFPEIDLKKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFRL3_micMur Microcebus murinus (lemur) ABDC01470170 synteny unknown two stop codons and a frameshift
MVHTLNCLVAVSQNMGIGKNGDLPWPPLRNEFKYFQKMTTTSSVEGKQNLVIMGKKTWFSIPEKN*PLKDRINVVLSREL*EPPQGAHFIAKSLDDALKLIEQPELANKVDMVWIVGGSAVYKEAKNQPGHLKLFVTRIMQDFESDTFFPEIDMKKYKLLSECPGVLSDVQEEKGMKYKFEVYERnd

>DHFRL4_tupBel Tupaia belangeri (treeshrew) AAPY01242529 several candidates but synteny unknown retrogene shown two frameshifts
MVRPLDCIVAVSQmGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNTVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPRGAHFLAKSLDDALKLIEQPELANQVDMVWIAGGGSVYKEAMNQPGHLKPFVTRIMQDFESDTFfPEIDLEKYKLLPGYPGVLSDDQEEKGIKYKFEVYEEN

>DHFR_musMus Mus musculus (mouse) AK031241
MVRPLNCIVAVSQDMGIGKNGDLPWPPLRNEWKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQPELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKKD

>DHFR_ratNor Rattus norvegicus (rat) NM_130400
MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELASKVDMVWVVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEIQEEKGIKYKFEVYEKKD

>DHFR_criGri Cricetulus griseus (hamster) NM_001244016
MVRPLNCIVAVSQNMGIGKNGDFPWPMLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELADKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFETDTFFPEIDLEKYKLLPEYPRVLPEVQEEKGIKYKFEVYEKKG

>DHFR_perMan Peromyscus maniculatus (deermouse) GH506523
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA

>DHFR_perPol Peromyscus polionotus (deermouse) GH462222
MVRPLNCIVAVSQNMGIGKNGDLPWPLLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWVVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPDIDLEKYKLLPEYPGVLSEVQEEEGIKYKFEVYEKKA

>DHFR_dipOrd Dipodomys ordii (kangaroo_rat) wgsBlast
MGRSLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDEALKLIEQPELNNKVDMVWIVGGSSVYKEAMNQPGHHKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSEVQEEKGIKYKFEVYEKID

>DHFR_speTri Spermophilus tridecemlineatus (ground_squirrel) ti|1005446275
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMMTTPSVEGKQNLVIIGRKTWFSIPEKNRPLNDRINIVLSRELKELPKGAHFLAKSLTDALKLTEQPELASKIDMIWIVGGSSVYKEAMIQPGHIKLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILSEVQEEKGIKYKFEVYEKTD

>DHFR_cavPor Cavia porcellus (guinea_pig) XM_003479518 Hystricognathi
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFGYFQRMTTTPSVEGKQNLVIMGRKTWFSLPEKSRPLKDRINVVLSRELKEPPQGAHFLAPSLDDALKLIEQPGLANEVDMVWIVGGSSVYKEAMERPGHIKLFVTRIMQEFESDTFFPEIDLKKYRLLPEYPGVPSEIQEEKGIKYKFEVYEKND

>DHFR_oryCun Oryctolagus cuniculus (rabbit) FJ687582
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINVVLSRELKEPPQGAHFLAKSLADALKLIEEPELAEKVDMAWIVGGSSVHKEAMNQPGHLRLFVTRIIQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_ochPri Ochotona princeps (pika) wgsBlast/ti|1497151195
MVRPLNCIVAVSQNMGIGRNGDLPWPPLRNEFKYFQRMTTTSTVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPPGAHFLAKSLDDALKLIEEPELAERVDMVWIVGGSSVYKEAMDKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGILPEVQEERGIKYRFEVYEKND

>DHFR_felCat Felis catus (cat) wgsBlast
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALELTEQPELAHKVDMVWVVGGSSVYKEAMNRPGHVRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDIQEEKSIKYKFEVYEKNN

>DHFR_canFam Canis familiarus (dog) XM_003432480
MVRTLNCIVAVSQNMGIGRNGDLPWPPLRNEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_vulVul Vulpes vulpes (fox) SRA|SRR094997.876315.2
MVRTLNCIVAVSQNMGIGRNGTVPWPPLRNEFKYFQRMTTNSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRDLKEPPQGAHFLAKSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMHEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_musPut Mustela putorius (ferret) AEYP01048652/AEYP01048654/AEYP01048651/SRA|SRR071690.159842.2
MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEknd

>DHFR_ailMel Ailuropoda melanoleuca (panda) XM_002916218
MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKFFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRDLKEPPQGAHFLAKSLDDALKLIEQPELANKVDIVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLDKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKND 

>DHFR_ursAme Ursus americanus (bear) GW292063 fragment
MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKFFQRMTTTSSVEGKQNLVIMGRKT

>DHFR_equCab Equus caballus (horse) XM_001504643
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTASSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALSLLEQPELANKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDFEKYKLLPEYPGVLSDIQEEKGIKYKFEVYEKND

>DHFR_vicPac Vicugna paca (vicuna) wgsBlast
MVRPLDCIIAVSQNMDMGKNGDLPWPplrNEFKYFQRMTTASSAEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLANSLDDALKVIEQPELTNKVDMVWIVGGSSVYKEAMDKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLLSEYPGVPSDVQEEKGIKYKFEVYEKNN

>DHFR_susScr Sus scrofa (pig) NM_001244064
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEYKYfQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDDALKLTEQPELKNKVDMVWIVGGSSVYKEAMNKPGHIRLFVTRIMKEFESDTFFPEIDLEKYKLLSECSGVPSDVQEEKGIKYKFEVYEKNN

>DHFR_turTru Tursiops truncatus (dolphin) DT661408
MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEYRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPQGAHFLAKSLDGALKLIEQPELTNTVDMVWIVGGGSVYKEAMNKPGHLRLFVTRIMQEFESDTFFPEIDLEKYKLIPEYPGVPSDVQEEKGIKYKFEVYEKNN

>DHFR_oviAri Ovis aries (sheep) GO728717
MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN

>DHFR_capHir Capra hircus (goat) JO612108
MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFKYFQRMTTVSSEEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDMVWIVGGSSVYKEAMNKPGHLRLFVTRIMQEFESDVFFPEIDFEKYKLLPEYPGVPSDVQEEKGIKYKFEVYEKNN

>DHFR_bosTau Bos taurus (cow) NM_001077883
MVRPLNCIVAVSQNMGIGKNGNLPWPPLRNEFQYFQRMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPKGAHFLAKSLDDALELIEDPELTNKVDVVWIVGGSSVYKEAMNKPGHVRLFVTRIMQEFESDAFFPEIDFEKYKLLPEYPGVPLDVQEEKGIKYKFEVYEKNN

>DHFR_myoLuc Myotis lucifugus (microbat) wgsBlast
MVRTLNCIAAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEEPELTNQVDMVWIVGGSSVYKEAMSKPGHQRLFVTRIMQEFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKNE
 
>DHFR_pteVam Pteropus vampyrus (macrobat) wgsBlast
MVLSLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSveGKRNLVIMGRKTWFSIPKKNRPLKDRINLVLSRELKEPPKGAHFLAKSLDDALKLIEQPELRSKVDMVWIVGGSSVYKEAMNKPGHIKLFVTKIMQEFESDIFFPEIDLGKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_eriEur Erinaceus europaeus (hedgehog) wgsBlast/ti|909141827
MVRPLNCMVAVSQNMGIGKNGELPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDAALKLIDQPELTNKVDRV----------EVMNRPGHIRLFVTRILQEFECDTFFPEIDLKNYRLLP--------------------------

>DHFR_sorAra Sorex araneus (shrew) wgsBlast
MVRPLNCIVAVSQNMGIGKNGELPWPPLRNEFTYFRKMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPTGAHFLAKSLDDALKLIEQPELTNKVDMVWIVGGSSVYQEVMNKPGHLRLFVTRIMQEFECDTFFPEIDLEKYKLLTDYPGVVSDVQEEKGIKYKFEVYEKKD

>DHFR_loxAfr Loxodonta africana (elephant) XM_003408117 error single trace TTTTTSSV
MVRPLNCIVAVSQNMGIGKNGDLP*PplRNEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRGLKEPPQGAHFLAKSLDDALKLIEQPELRNKVDMVWIVGGSSVYKEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLEKYKRLSEYPGVLSDVQEEKGIKYKFEVYEKKD

>DHFR_proCap Procavia capensis (hyrax) wgsBlast
MVRPLSCIVAVSQNMGIGKNGDLPWPPLRNEFKHFRTMTSTPSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLAKSLDDALKLIEQPELTNKVDLVWIIGGSSVYKEAMSQPGHLTLFVTRIMQEFESDTFFPEIDLEKFKLLPEYPGVPSDVQEEKGIKYKFEVYEKKD

>DHFR_dasNov Dasypus novemcinctus (armadillo) AAGV020342833
mVRPLSCYDAVYQNMGIGKNGDMPWPPLRNEYKYFQKMTTVSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELREPPQGAHFLAKSLDDALKLIEQPELADKVDMIWIVGGSSVYK---------------------------------------YPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_choHof Choloepus hoffmanni (sloth) genome/blat many pseudogenes
-----------------------------NEFKYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELK------------------------------------------EAMNRPGHLRLFVTRIMQEFESDTFFPEIDTEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

>DHFR_monDom Monodelphis domestica (opossum) XM_001363581
MVRQLNCIAAVSKNMGIGKDGDLPWPLLRNEFKYFQKMTTTPSVEGKQNLVIMGKKTWFSIPEKCRPLKDRINVVLSRELKEPPQGAHYLASSLDNALNLLELPELADKVDMVWIVGGSSVYKEAMNKPGHQRLFITRILQEFESDTFFPEIDLKKYKQLQDYPGVSPDVQEEKGIQYKFEVYEKHE

>DHFR_macEug Macropus eugenii (wallaby) wgsBlast
MAPTINCIVAVAQNMGMGKNGDLPWPPLRKEFQYFQKMTTTPSVEGKQNLLIMGKKTWFSIPEKNRPLKDRINLVLSRELKDPPLGAHYLAKSLDDALDLLRLPELANTVDMVWVVGGSSVYKEAMNKPGHQRLFVTRILQEFESDTFFPEIDLKRYKLLPEYPGVLTDVQEEKGIQHKCEIHEKHD

>DHFR_sarHar Sarcophilus harrisii (tasmanian_devil) AFEY01286966/AFEY01033989/AFEY01359230
MVRPINCIAAVSQNMGIGKNGDLPWPPLRNEFNYFQKMTTTSSVEGKQNLLIMGKKTWFSIPEKHRPLKDRINLVLSRELKEPPPGAHYLAKSLDDALDLLELPELADKVDMIWVIGGSSVYKEAMEKPGHQRLFVTRILQEFECDTFFPEIDLKKYKLLPEYPGVLTDIQEEKGIQYKFEVYEKHD

>DHFR_triVul Trichosurus vulpecula (brushtail) EG594753
MVRTINCIVAVSQNMGIGKNGDLPWPPLRNDFKHFQKMTTIPSVEGKQNLLITGKKTWFSIPEKSRPLKDRINVVLSRELKEPPPGAHYLAKSLDDALDLLELPELANKVDMIWVVGGSSVYKEAMNKRGHNRLFVTRILQEFESDTFFPEIDLKRYKLLXEYPGVLTDV

>DHFR_ornAna Ornithorhynchus anatinus (platypus) XM_001512186
MGPLLNCIVAVAKNGGIGNKGDLPWPALRNEFRYFQKMTTTPTVKGKQNLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLHLPELASKVDMVWIIGGSSVYKEAMEKGGHQRLFVTRILQDFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI

>DHFR_tacAcu Tachyglossus aculeatus (echidna) SRA:EUGXWLM02GHH9L/EUPZL4S02GL94B
MGRPLNCIAAVAKNGGIGNKGDLPWPPLRNEYRYFQKMTTTPTVEGKENLVIMGKKTWFSIPEKSRPLKDRINIVLSRELKEPPQGAHYVAKSLDDALNLLNLPELASKVDMVWIIGGSSVYKEAMEKPGHQRLFVTRILQEFESDTFFPEIDLEKYKLIPEYPGVPADTQEEKGIQYRFEVYEKHI

>DHFR_galGal Gallus gallus (chicken) NM_001006584 
MVRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTAVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEENGIQYKFEVYQKSVLAQ

>DHFR_melGal Meleagris gallopavo (turkey) genome/blat
------------QNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVE------------------------------------EAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLKEYPGVPADVQEENGIQYKFEVYQKSVLAQ

>DHFR_lagLag Lagopus lagopus (ptarmigan) GW704257
MVRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYQKSVLAQ

>DHFR_anaPla Anas platyrhynchos (duck) scaffold675
MVRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADVQEENGIQYKFEVYEKTVLAQ

>DHFR_taegut Taeniopygia guttata (finch) XM_002190440
MPRSLNSIVAISQNMGIGKDGRLPWPPLRNDYKYFQRMTSASPVEGKKNAVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELQSKVCMVWIVGGSSVYKEAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKTVVP

>DHFR_ficHyp Ficedula hypoleuca (flycatcher) SRA|SRR029160.210264.2
MVRSLNSIVAVSQNMGIGKDGRLPWPPLRNEYKYFQRMTSTPRVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELKEAPEGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPIHHRLFVTRILKEFESDTFFPEIDHKDYKLLTEYPGVPADIQEENGIQYKFEVYEKAVV*

>DHFR_mrlUnd Melopsittacus undulatus (budgerigar) SRA|SRR029329.119695.2
MVRSLNSIVAVCQNMGIGKDGSLPWPPFRNEYKYFQRMTSTSQVEGKQNALIMGKKTWFSIPEKNRPLKDRINIVLSRELQETPKGAHYLSKNLDDALALLDSPELKSKVDMVWIVGGTSVYKAAMEKPINHRLFVTRILQEFESDTF

>DHFR_allMis Alligator mississippiensis (alligator) genome/blat 
MVSSLNAIAAVSQNMGIGKNGTLPWPPLRNEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKT

>DHFR_croPor Crocodylus porosus (crocodile) genome/blat composite seq with alligator
MVSSLNAIAAVSQNMGIGKNGTLPWPPLRNEFKYFQRMTTTPSVPGKQNVLIMGKKTWFSIPEKNRPLKDRINIVLSRELAEVPKGAHHLARSLDDALVLLDSPELKNKIDLVWIVGGSSVYKAAMEKPINQRLFVTRILHEFESDTFFPEIDLEKYRLLPEFPGVPADVQEENGIHYKFEVYEKTV

>DHFR_chrPic Chrysemys picta bellii (turtle) AHGY01047456 AHGY01047458
MVRPLNCIAAVCQNMGIGKNGDLPWPPLRNEFKYFQRMTTTTTVEGKQNVLIMGKRTWFSIPEKNRPLKDRINIVLSRELKETPKGAHYLAKSLDDALVLLDSPELKSKIDMVWIIGGSSIYKAAMEKPVHQRLFVTRILHEFESDTFFPEIDLEKYKHLPEYPGVPADVQEENGIRYKYEVYEKTV*

>DHFR_anoCar Anolis carolinensis (lizard) genome/blat XM_003216272
MVLSLNSIAAVCQNMGIGKNGQLPWPPLR 2NEFKYFQKMTMTPTQEGKQNVVIMGKRTWFSIPEKNRPLKDRINIILSREMKEVPKGAHYLARSLDEALDLLQSPELENKIDLVWIVGGSSVYK AAMDKPIHHRLFVTRILHDFESDTFFPEIDLEKYKLIPSYAGVPTDIQEENGIRYRFEVYEKNI

>DHFR_pytMol Python molurus (python) AEQU011279324/AEQU010134262/AEQU010204446/AEQU010142865/AEQU010435622/AEQU010656196
MVASLHSIVAVCNNMGIGKDGKLPWPPLRNEFKHFQKMTMTTKEEGKQNVVIMGKKTWFSIPEKHRPLKNRINVVLSKELKDVPEGAHYLAKSLEEALDLLETSEMERKVDKVWIVGGSSVYK AAMEKPIHHQLFVTRIMHDFESDTFFPEIDLKKYKLLPKYTGIPTDIQEENGIQYKFEVYENTI

>DHFR_ambMex Ambystoma mexicanum (axolotl) CN050864
MGRILNAIVAVCPSMGIGKDGNLPWPILRNEFKYFQRMTMTATQEGKQNVVIMGRKTWLSIPEKNRPLKGRINIVLSRELTAPPPGAHYLANSLDGALELLETRELAGKVDLLWIIGGSSLYRELIGRPHHQRLFVTRILKEFECDTFLPEFDLEKYRLLPEYPGVSLGIQEENGIQYKFEVYEKVEEA

>DHFR_xenTro Xenopus tropicalis (frog) CT025223
MRNPFLHAVVAVCPPNQGIGKEGSLPWPLLRNEFKHFQRLTMTPTVEDKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLAEAIDLLEDPELKDKVDLVWVIGGSSLYQELMEKPVNQRWFVTRILQEFECDTYLPEIDLNSFRLLPEYPGISPELQEENGVQYKFEVYEKIV

>DHFR_xenLae Xenopus laevis (frog) NM_001095037
MRNQFLHAVVAVCPPNQGIGKGGSLPWPLLRNEFKHFQRLTMTPTVEGKKNVVIMGRKTWFSIPEKNRPLKERINIVLSKELKEPPTGAHYLSKSLTEAIDLLEDPELKDKVDLVWIIGGSSLYQELMEKPINQRWFVTRILQEFECDTYLPEINLNSFQLLPQYPGISPELQEENGVQYKFEVYEKIV

>DHFR_ranCat Rana catesbeiana (bullfrog) GO463031
---------------------GGTLPWPLLRNEFKHFQRLTMTPTTEGKQNVVIMGRRTWFSIPEKNRPLKGRINIVLSRELTEPPNGAHYLAKSLDEAVHLLKTPEMKEKIDLIWIIGGSSLYKEVMDKPVSQRLFITRIQQEFECDTFLPDVDLQSYKLLPEYPGISSEMQEENGIQYKFEVYEKML

>DHFR_latCha Latimeria chalumnae (coelocanth) AFYH01150805/AFYH01150806/AFYH01150807/AFYH01150815/AFYH01150814
MGAARLLNSIVAVCPNLGIGKDGNLPWHPKRLSNEFRYFQKMTTTPTVEGKQNVVIMGRKTWFSIPEKNRPLKGRVNVVLSRELQEAPEGAHYLARDLDSALKLLESPELADRVDLVWIIGGSALYKEALEHPSQHRLFVTRVLQQFECDTYLPEINFNKFTLLPEFPGVSSAIQEESGIQYKFEVYEKNS
 
>DHFR_gadMor Gadus morhua (cod) FF410604 novel insertion 3 transcripts
MSRVLNCIVAVCPDAGIGYKGDLPWHPTRLNNEFKHFRRLTVTrtPGAEDKQNVVIMGRKTWNSIPEKNRPLNDRINIVLSRQLEAPPSGAHHLARDLPSALRLLDVPEGLLADQVWLIGGSSMYSEVMETPCPRRLFVTRILKQFPSDTFFPDISPAQFRLLPEYPGVSSELQEENGIQYRFQVYQSVDP

>DHFR_tetNig Tetraodon nigroviridis (pufferfish) CR710339
MARVLNAIVAVCPDLGIGRNGDLPWHPIRLDNEFKHFRKMTSTPSVNGKQNVVIMGRKTWFSIPEKHRPLANRINIVLSRRSREPPAGAHHLAHDLSSALQLVETQLAEQADQVWVIGGSSLYQEMMERPGTSRLFVTHVLKQFDCDTFLPEIRPQRYRLLPQFPGVPQELQEEKGIQYRYQVYESLEP

>DHFR_hipHip Hippoglossus hippoglossus (halibut) EB031730 
MSRILNGIVAVCPDLGIGNRGNLPWHPVRLSNEFKHFRSMTATPSEKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKAPPAGAHHLSADFSSALRLVDTELADQADQVWVIGGSSLYKEMMESPGTRRLFVTRILKQFDCDTFLPEISPDKYRQLPEFPGVPQELREENGIQYRFEVYESIEP

>DHFR_solSen Solea senegalensis (sole) FF286406 
MSRVLNGIVAVCPDMGIGMTGNLPWHPVRLSNEFKHFRTMTATSSVKGKKNVVIMGRKTWFSIPEKNRPLNNRINIVLSRQCKLPPAGAHHLAADFSSALRLVETELADEADQIWIIGGSSLYKEMMESPGIKRLFVTRILKQFECDTFLPEISLDKYRLLPEFAGVSQELQEENAIQYRFEVYESVEQ

>DHFR_oreNil Oreochromis niloticus (tilapia) GR620119
MPRVLNAIVAVCPDRGIGNKGNLPWHPIRLSKEFAHFRKMTATPSVKGKQNVVIMGKKTWYSIPEKNRPLSNRINIVLSRECKVPPAGAHYLASDFSSALRLIDTELADQADQVWVIGGSSLYKEMMGSTGMRRLFVTQILKQFECDTFLPEISLDKYRLLPEFPDVPQELQEENGIQYRFKVYESIQE

>DHFR_dicLab Dicentrarchus labrax (seabass) FM022259 
mSRILNGIVAVCPDLGIGMNGNLPWHPVRLGNEFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSKECKVPPAGAHHLAADFNSALRLVETELADQADQVWVIGGSSLYKELMESPGTRRLFVTRILKQFECDTFLPEICPDKYQLLPEFPGVSQELQEENGIQYRFEVYESTEQ

>DHFR_perFla Perca flavescens (perch) GO655828 
MSRILNGIVAVCPDLGIGNNGNLPWHPVRLNNEFKHFRTMTATPSVNGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRECKAPPAGAHHLARDFSSALRLVDTELAQQADTVWVIGGSSLYKEMMESPGTRRLFVTRILKQFECDTFLPEITPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIQQ

>DHFR_spaAur Sparus aurata (seabream) FM155123 
MSRIVNGIVAVCPDLGIGNNGNLPWHPVRLNREFKHFRTMTATPSVKGKQNVVIMGRKTWFSIPDKFRPLNNRINIVLSRECKVPPAGAHHLAADFSSALSLVDTELADQADQVWVIGGSSLYKELMESTRTSRLFVTRILKQFECDTFLPEISPDKYSLLPEFPGVPQGLQEENGIQYKFEVYERIKQ

>DHFR_gasAcu Gasterosteus aculeatus (stickleback) ENSGACT00000004939 
MSRVLNGIVAVCPDLGIGCHGNLPWHPLRLSNEFKHFRRMTATASVKDKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKAPPAGAHHLAPDFSSALRLVDTELAEQADQVWVIGGSSLYKELLESPGTRRLFVTRILKQFECDTFLPEICPDRYRLLPEFPGVPEELQEENGVQYRFEVYESIDV

>DHFR_oryLat Oryzias latipes (medaka) BJ496706
MTRTLNGIVAVCPDLGIGKGGNLPWHPLRLSKDFALFRKMTSTPLVAGRQNVVIMGRKTWFSIPEKNRPLQNRINIVLSRQLKAPPAGAHFVAADFAAALRLVDSELAERADQVWIIGGSSIYXEMMESPAPRRLIVTRVQKQFECDTFFPEIRPEQFRLLPEFPGVSQELQEENGVSYKFEVYESIQQ

>DHFR_anoFim Anoplopoma fimbria (sablefish) BT082890
MSRVLNAIVAVCPDLGIGRNGDLPWHPVRLNNEFKHFRRMTSTPSVEGKQNVVIMGRKTWFSIPEKNRPLNNRINIVLSRELKTPPAGAHHLAQDFNSALRLVDMKLAEQVDQVWVIGGSSLYKELMESQGIRRLFVTQILKQFECDTFLPKISPDKYRLLPEFPGVPQELQEENGIQYRFEVYESIEQ

>DHFR_esoLuc Esox lucius (pike) BT079933
MSRVLNCIVAVCPNMGIGNKGNLPWHPKRLNNEFKYFQKMTMTPFVEGKQNVDIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPPGAHHLASDFSSALRLLDTAEMAERVDQVWVIGGSSLYKEMMESEGIRRLFVTRILQQFESDTFLPEINQDKYRLLPEFPGVPQDVQEESGIQYRFEVYESVDH

>DHFR_salSal Salmo salar (salmon) BT047529
MSRVPNCIVAVCPDVGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNVVIMGRKTWFSIPERNRPLKNRINIVLSRELKKPPAGAHHLASDFRSALCLLDMAEMAERADQVWVIGGSSLYKEMMESQGTRRLFVTRILQQFKSDTFLPEINQDQYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ

>DHFR_oncMyk Oncorhynchus mykiss (trout) EZ786723
MSRVLNCIVAVCPDMGIGNNGNLPWHPKRLNNEFKYFQKMTMTSSVEGKQNAVIMGRKTWFSIPERNRPLKNRINIVLSRELKEPPAGAHQLASDFRSALRLLDTAEMAERVDQVWVIGGSSLYEEMMESQGTRRLFVTRILQQFKSDTFLPEINQDKYRLLPEFPGVPQDLQEEAGLQYRFEVYESIDQ

>DHFR_danRer Danio rerio (zebrafish) NM_131775
MSRILNCIVAVCPDMGIGKNGNLPWHPIRLSNELKHFQKMTMTPSDEGKKNVVIMGRKTWFSIPAAHRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSGELEKLVDQVWIIGGSSLYKEVMERSGHRRLFVTRILKQFDCDTFIPNFDMDKYKLLPEFPGVPVGLQEDNGVQYLFEVYESIKH

>DHFR_cteIde Ctenopharyngodon idella (carp) CK233011 
MSRILNCIVAVCPDMGIGRKGNLPWHPIRLSNEFKHFQKMTMTPSVEGKKNVVIMGRKTWFSIPAQNRPLKNRINIVLSRELKTAPEGAHYLASDLSSALHLLDSSELEKQVDQVWIIGGSALYKEVMERSGPRRLFVTRVLKQFDCDTFIPEINMDKYKLLPEFPGVPTGLQDENGVQYVFECMIVS

>DHFR_cypCar Cyprinus carpio (carp) CA968873 
MSRILNSIVAVCPDMGIGKNGNLPWHPIRLSNEFKHFQKMTMTPLVEGKKNVVLMGRKTWFSIPAANRPLKNRINIVLSRELKTAPEGAHYLASDFSSALHLLDSSELEKQVDQVWIIGGSALYKEVMESSGPRRLFVTRVLKQFDCDTFIPDINMDKYKLLPEFPGVPTGLQEENGVQYVFEVYESTDH

>DHFR_ictPun Ictalurus punctatus (catfish) FD330522 
MGRVLNCIVAVCPDMGIGRNGNLPWHPIRLSKEFKHFQKMTMTPTVEGKKNVVIMGRKTWFSIPAQNRPLKNRINVVLSRELKTAPEGAHYLAADFSSALRILENAELEAQADQVWIIGGSSLYKEVMESSAHQRLFVTRILKQFDCDTFIPNIDLDKFRLLPEFPGVPAGLQEENGLQYVFEVYESVEQ

>DHFR_leuEri Leucoraja erinacea (skate) EE989413 
MTRLINSIVAVCPNMGIGNNGNFPWHPIRLSKEFKHFQRMTSTPSVEGKCNAVIMGRKTWHSIPEKNRPLKNRINIVLSRELKEVPEGAHYLAPDLDSALALLDSSDLQDKVDLVWIIGGSALYKEALESPASHRLFVTRILQDFDSDTFIPEINLNKFKLLPEFPDVPADIQEENGIRFKYEVYERISPV

>DHFR_squAca Squalus acanthias (dogfish) DV497998 
mPRLVNCIVAVCPNMGIGKDGNFPWHPIRLSKELKHFQKMTATPSVEGKRNAVIMGRKTWFSIPEKHRPLKDRLNIVLSRELKELPHGANYLAHDLDSALAHLDSSELQDKVDLVWIIGGSTLYKEAMESPVSHRIFVTRILKDFESDTFLPEMDLNKFKLLPKFPDVPTEVQEDNGIQYKFEVYERIAHA

>DHFR_eptBur Eptatretus burgeri (hagfish) BJ646201
MAQNPVNVIAAVLPNMGIGWKGGLPWHSKSLVKEMKHFTRLTSAAAEGKQNAVVMGRKTWESIPEKFRPLRNRINVVLSRTLSHLPLGAHYLATDFSNALAQLSESELCCRIDHIWIIGGSSIYKEALLHPACHRIFITRIMHDFEADTFFPHIDSKRFSLCQSYPGIPEEIQEENGVQYKFEVYEVVAK

>DHFR_petMar Petromyzon marinus (lamprey) Contig6033:1-27,714
GKQNVVIMGRKTWFSIPEKKRPLKNRINIVLSRELKCLPEGAHYLVPDLNSALSLIDSTPAIQEKLDGVWILGGGGVYKEAMEHSACRRLFITRVLQTMEADAFFPDIDADKFKLLP

Urochordate, cephalochordate, hemichordate and echinoderm reference sequences

>DHFR_cioInt Ciona intestinalis (tunicate) F971791 dozens of transcripts
0 MPAKDIQIHSVVACCNNGGIGFKGRLPWRLP 2
1 KEMKYFKRITTGEVEEFG GRRNaiIIGRKTWESIPKSFKPLKDRINIVISRTLDKDCEGP 0
0 DLVVNSLDELIdLLHSKPWCDKINQVFNIGGNEIYK 0
0 LIHNSQYCGKIYLTRVLADlQCDTFLENLDDNFTKLPTEG 2
1 FPEVPQGIQMDKNDLQWKVEVYEKKTT* 0

>DHFR_cioSav Ciona savignyi reftig_155
0 MPAKELKIHSIVACCNNRGIGNKGRLPWRLP 2
1 KEMKHFTSLTTGDVENLN GKRNAVVVGRKTWESIPKSFKPLKNRVNIVISRSLSKEYEGP 0
0 DAVVNSLEELVQLLQSDHWSNRINQVFNIGGSEVYK 0
0 LINNSKYCGTIYLTRVLADFECDTFLDKFDDTFKQLDTTK 2
1 FPEVPQGEQMDKNDISWRVEVYVKKS* 0

>DHFR_halRor Halocynthia roretzi (squirt) DB618016/EY457317
0 MKIHAVVACCNNRGIGKDGTLPWRLP 2
1 KEMAHFQKITKGSPPK  GKKNAVVMGRKTWEEIK   RPLKDRYNIVMSRSLSEKLKGM 0
0 DALVRSLGELEDLLSSGEWTGEINEVFNIGGAEICR 0
0 LINGSSLCGRLCVTQMVTDFECDAFLPEIDDGEYLR LDE 2
1 YPEDVATLLPDGEVVEANGVKWAVEVYEAI* 0

>DHFR_botSch Botryllus schlosseri (tunicate) HO068783
0 MEIHTVVVCDDNWGISKDGCLPFGTK 2
1 KEAAHFYRFIADTSHLPP GRKNVLIMGRKTWTE ETPADAPQSRSVTYNFLLSKTLKERPEEV 0
0 DGIVSSVEEFVTLMESPRWKDKIGRIYNIGGGQACE 0
0 AIYASKYCGRAYMSLVRGDFHCDTFAPNLD

>DHFR_molTec Molgula tectiformis CJ399083 poor quality fragment
MGRNTWNSIPSKFRPlKnRWNIVLSRTlKDPyDGM
HAVVSSlDQlYKlLQDEAWKDKVNEIFCIGGAQVYE
VIDNSPYIGLiFMTRIRKKYDCDTFYSEVNKDKFILLKSCP

>DHFR_oikDio Oikopleura dioica (tunicate) CABV01002330/FP801526 completely different intronation from Ciona
0 MNKSGWNMILAADIKGGIGLRNDLPWRIPQDLKHFQMLTKGTPNQESVVVMGRNTWQSIPEKFRPLK 0
0 GRVNVVITNTLESENFIKCTSLEAAIAYINENHVNATKWIIGGAtLYNRGLKENFIDEVYLTRIQGDY 1
2 NCDTYVKDLYKILEVDYRLETSPKFDSKEVIGESGIKYAFERYVKNSGI* 0

>DHFR_braFlo Branchiostoma floridae (amphioxus) ABEP02022512/XM_002597864/BW702700 triplicated contig misassembly
0 MKTKKLSLVVAACNNMGIGVDGKIPWTLR 2
1 GDMKFFSRLTSGTEEA 1
2 GKQNAVVMGRKTWFSIPDRFRPLPKRLNVVLSRNLT 2
1 TPPEGAHHLAGSLEEAVKMLTETTVADTIDKVFIIGGNSVYK 0
0 DALSHPCCHRVYLTRVYKDFHCDTFFPNMDDTFKLVS 2
1 DPTIPSEMQEENGIEYKYEVYEKVA* 0

>DHFR_sacKow Saccoglossus kowalevskii (acornworm) ACQM01022605 exons 2-3 fused
0 MKKISLVAAACNNMGIGKNGDLPWRLR 2
1 KEMSFFTKVTSETKED GKQNAVVMGRKTWFSIPEKYRPLAGRYNVILSKNLK 2
1 ECPSGADMLSESLDDAIKQLSEPPLVDKIDKVYIIGGSSVYK 0
0 ESLESAACYRIYLTRVMADFDCDTFFPEFDTNLFNLVS 2
1 DPDVPSEIQEEKGIQFKYEVYEKK* 0

>DHFR_balCla Balanoglossus clavigerus (acornworm) FN986971
mQKISPVAAACNSMGIGKNGNLPWRLRKEMKYFTNVTSETVEEEKQNAVIMGRKTWFSIPEKYRPLNNRFNVILSNTLKECPAGADLLSPSLEEAVKLLSESPLEGKIDKVFIIGGSSVYKESIQSSLCHRIYLTRIMADFDCDTFFPNFDTNIFKLISDPSIPSEIQEEKGIQFKYEVYERQDV

>DHFR_strPur Strongylocentrotus purpuratus (urchin) AAGJ04143548  exons 2-3 fused
0 MAEKKLNLIAAACTSkGKMGIGINGNLPWRLR 2
1 QEMAYFERLTKTAQME GMKNAVIMGRKTWDSIPEKFRPLKDRVNVVLSNSLT 2
1 ECPPGADHLCSSLNEAVKLFSSPPLSETVDMVWITGGSAVYK 0
0 DGIDSPHCHRIYLTRIMKEIECDTFFPEFDLDRFKLVT 2
1 DPAVDQDTQEEKGIQYKFEIYESSS* 0

>DHFR_parLiv Paracentrotus lividus (urchin) AM567148
MADKRLNLIAAACTSNGKMGIGINGNLPWRLRQEMAYFERLTKTSQMEGMKNAVVMGRKTWFSIPEKFRPLKDRVNVVLSSSLTECPPGADHLCSSLSEAVKLLSSPPLSQTVDMVWITGGSSVYKDGIDSPLCHRIYLTRIMKEIECDTFFPEFDLDRFKLVTDDAVSQDIQEEKGIQYKFEIYESS

>DHFR_lytVar Lytechinus variegatus (urchin) JI431194
MAEKKLNLIAAACTSSGKMGIGINGNLPWRLRQEMAYFERLTKTPQLEGMKNAVIMGRKTWFSIPEKFRPLKDRINVVLSNTLTECPSGADHLCSSVSEAVKLLSCPPHSQTVDMLWIIGGSAVYKAGIESPCCHRIYLTRVLKEIECDTFFPEFDLEKFKLVVDPAVSQDIQEEKGIQYKFEIYENSS

>DHFR_patPec Patiria pectinifera (starfish) DB389444/DB385866 also called Asterina
MAGQKQcNLIVAACKCKDSLGIGINGTIPWKLRTDMKFFSTQTSTTAEN DKKNAVIMGRKTWLSIPDKFRPLPNRVNIVLSKTLSECPAGADHLCRSLDHAVKLLSEPPVADGIDVVWIIGGSSVYKEAMESSLCHRIYLTRIHSQFECDTFMPEIDTSKFKVVSDPAVSGERQKENDIEFNFEIHQNIQHTSTS

Protostome reference sequences

>DHFR_droMel Drosophila melanogaster (fruitfly) NM_001043255 Diptera; Muscomorpha 2-6 fused 
MLRFNLIVAVCENFGIGIRGDLPWRIKSELKYFSRTTKRTSDPTKQNAVVMGRKTYFGVPESKRPLPDRLNIVLSTTLQESDLPKGVLLCPNLETAMKILEEQNEVENIWIVGGSGVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIPDSFREVAPDSDMPLGVQEENGIKFEYKILEKHS

>DHFR_droSim Drosophila simulans (fruitfly) XM_002103136 Diptera; Muscomorpha 2-6 fused
0 MLRFNLIVAVCENFGIGIKGDLPWRIK 2
1 SELKYFSRTTKRTSDPTKHNAVVMGRKTYFGVPESKRPLPDRLNIVLSTTLQESDLPKGVLLCPNLETAMKILEEQNEVENIWIVGGSGVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIPDSFREVAPDSDMPLGVQEENGIKFEYKVLEKQS
	
>DHFR_droSec Drosophila sechellia (fruitfly) XM_002041171 Diptera; Muscomorpha 2-6 fused
0 MLRFNLIVAVCENFGIGIKGDLPWRIK 2
1 SELKYFSRTTKRTSDPTKHNAVVMGRKTYFGVPESKRPLPDRLNIVLSTTLQESDLPKGVLLCPNLETAMKILQEQNEVENIWIVGGSGVYEEAMASPRCHRLYITKIMQKFDCDTFFPAIPDSFREVAPDSDMPLGVQEENGIKFEYKVLEKQS
	
>DHFR_droEre Drosophila erecta (fruitfly) XM_001980159 Diptera; Muscomorpha 2-6 fused
0 MIRFNLIVAVGENFGIGYKGDLPWRIK 2
1 SELKYFSRITKRTNDPSKHNAVVMGRKTYFGVPESKRPLSNRLNVVLSTTLQENELPEGVLLCSNLETALKVLENKNDVENVWIVGGSGVYKEAMASPRCHRLYITKILHKFECDTFFPAIPDSFREVAPDSDIPLGVQEENGIHFEYKVLEKQSK

>DHFR_droYak Drosophila yakuba (fruitfly) XM_002097560 Diptera; Muscomorpha 2-6 fused
0 MLSFNLIVAVCENFGIGINGNLPWRIR 2
1 SELKYFSRTTKRTSDPSKHNAVVMGRKTYFGVPEGKRPLPDRLNIVLSTTLQENELPKGVLLCRNLEAAMKALEDKNEVENIWIVGGSGVYEEAMASTRCHRLYITKIHEKFECDTFFPAIPDSFREVAPDSDTPLGLQEENGIKFEYKVLEKQ

>DHFR_droAna Drosophila ananassae (fruitfly) XM_001954865 Diptera; Muscomorpha 2-6 fused
0 MRRFSLIVAVSENLGIGIKGDLPWRIK 2
1 SELKYFSRTTKRTSDPSKRNAVIMGRKTYFGVPESKRPLPNRLNIVLSTTLQPEDLPKDVMLCPNLEAAMKHLEGDDQIENVWVVGGSGVYAEAMDSNRCHRLYITRIHQKFECDTFFPTIPESFREVAPDPDTPAGVQEENGIEYEYKVMEKETK

>DHFR_droPse Drosophila pseudoobscura (fruitfly) XM_001359349 Diptera; Muscomorpha 2-6 fused
0 MLRFNLIVAVSENFGIGLKGDLPWRLK 2
1 SELKYFSRTTKRTSDPNKRNVVIMGRKTYFGVPASKRPLPERLNIVLSTTLKPAELPSDVVLCHSLEEAMKHLEEETLRDQIENVWIVGGSGVYEEAMASSRCNRLYITKIQQNFECDTFFPSIPDSFREVPPDADTPVGVQEENGIKYEYKVLEKRE
	
>DHFR_droPer Drosophila persimilis (fruitfly) XM_002017170 Diptera; Muscomorpha 2-6 fused
MLRFNLIVAVSENFGIGLKGDLPWRLK 2
1 SELKYFSRTTKRTSDPNKRNVVIMGRKTYFGVPASKRPLPERLNIVLSTTLQPAELPSDVVLCPSLEEAMKHLEEETLRDQIENVWIVGGSGVYEEAMASSRCNRLYITKIQQNFECDTFFPSIPDCFREVPPDADTPVGVQEENGIKYEYKVLEKRE

>DHFR_droWil Drosophila willistoni (fruitfly) XM_002070058 Diptera; Muscomorpha 2-6 fused
0 MLRFNLIVAVCENSGIGLKGDLPWRIR 2
1 SELKYFSNTTKRRLDSSKRNVVIMGRKTYFGVPPSKRPLPDRVNVILSSTLKSNELPNEVLIYSSLEGAMQELEKQKDQIENVWIVGGSSVYAESMASPRCHRLYLTKIYAEFECDTFFPKIPESFREVSPDKDTPPGIQEENGLKYEYKVFEKQE

>DHFR_droMoj Drosophila mojavensis (fruitfly) XM_001998634 Diptera; Muscomorpha 2-6 fused
0 MLKYNLIVAVCENFGIGFKGDLPWRLK 2
1 SELKYFSRRTKRINDPSKRNVVIMGRKTYFGVPPSKRPLPDRLNIVLSTTLTQKELPEGVLLCGSLDEAMQTLEKKPLCDQVENIWIVGGSGVYAEAMESPRCHRLYITKIQERFECDTFFPHIPASFHEVELTEDTPHGVQEENGIKFEYKILEKREHII

>DHFR_droVir Drosophila virilis (fruitfly) XM_002056347 Diptera; Muscomorpha 2-6 fused
0 MLRYNLIVAVCENFGIGLKGDLPWRLK 2
1 SELKYFSRTTKRTRDPAKRNVVIMGRKTYFGVPPSKRPLPDRLNIVLSTTMSRAELPNDVLLCRSLEEAMKMLESESEPWCDQIENIWIVGGSGVYEEAMASPRCYRLYITKIQEQFECDTFFPNIPADFHEVPLDEDTPHGVQEENGIKYEYKILEKQ

>DHFR_droGri Drosophila grimshawi (fruitfly) XM_001990307 Diptera; Muscomorpha 2-6 fused
0 MLKFNLIVAVCENFGIGFKGNLPWNLK 2
1 SELKYFSRTTKRVCDPSKRNVVIMGRNTYMGVPPSKRPLPDRLNIVLSTTMSAADLPKEVLLCRSLNEAMETIESPEWCKQVENVWIVGGSRVYAEAMDSERFHRLYLTKIHEKFECDTFFPEIPDCFKEIPLDEHTPDGIQEEDGIKYEYKILEKQ

>DHFR_gloMor Glossina morsitans (tsetse_fly) EZ423753 Diptera; Muscomorpha
MLKFNLIVAVSKNFGIGLKGGLPWELKSELRYFSELTKRVFDSTKRNVVIMGRKTYFGIPLNNRPLRNRLNIVLSTTLNKVGELPEEVLLQPNLEAAMKFLEDNNTLKSNIENIWIIGGASVFKEAMASKRCHRLYITEIQSSFESDVFLPTIPNDFEQIIPGPEVPQTVQVENCICFRYKVLEKRE

>DHFR_haeIrr Haematobia irritans (buffalo fly) AY099474 Diptera; Muscomorpha
MLKFSLIVAVCENFGIGIKGDLPWLLKSELKYFSTTTKRVHDPSKRNVVIMGRKTYFGVPESKRPLQQRLNIVLSTTLKQSDLPKDVLLYPNLESAMSFLENEKDINQQIETVWIVGGSGVYKEAMASPRCHRLYLTKIYAKFDCDTFFPDIPADFKEVDLDPETPKGIQEENNITYEYKILEKQDHK

>DHFR_sarCra Sarcophaga crassipalpis (flesh_fly) EZ609788 Diptera; Muscomorpha
MLKFSLIVAVCENFGIGIKGDLPWKIKSELKYFSSTTKRVRDPSKRNVAIMGRKTYFGIPESKRPLPERLNIVLSTTLKPSDLPSDVLLYPNLESAMKMLEENEKLKQEIETVWIVGGSGVYAEAMASQRCHRLYLTKIQASFECDTFFPKIPDDFVEVELDEETPKGIQEENNIKYEYKILEKKT

>DHFR_culQui Culex quinquefasciatus (mosquito) XM_001864570 Diptera; Nematocera exons 2-6 fused
0 MKKFSLIVAVCSNGGIGIKGDLPWRLR 2
1 SELRHFARMTKRVADPGKRNAIIMGRKTYFGIPEGRRPLPDRLNVVLTRDPASNTYPEGVLVCTSLEDALAKIQDLVENIWIIGGSAVYKEAMESRHCHRIYLTEILAPFECDAFFPEIGKEFRLVGNDADLAEEVQEENGVRFQYKIYEKKDID

>DHFR_anoGam Anopheles gambiae (mosquito) XM_319471 Diptera; Nematocera exons 2-6 fused
0 MSKKFSCIVAVCENRGIGINGDLPWKLK 2
1 QELKYFSHTTKKVNDADKRNAVIMGRKTYFGVPESKRPLPERLNIVLTRNAAAYTFPPDVLVCGSLQEALHKLDSTDHGEHIENIWVVGGNSVYKEAMESDRCHRIYLTEIKKQFDCDAFFPEIPNSFMVIDNDPDTPSGVQEENGIQYVYKIYENKQQ

>DHFR_aedAlb Aedes albopictus (mosquito) X60192 Diptera; Nematocera exons 2-6 fused
0 MKKFSLIVAVCANGGIGIKGDLPWRLR 2
1 QELKYFSRMTKKIQDSGKRNAIIMGRKTYFGVPESKRPLPERLNIILTRDPSANAYPSEVMVCTSMQEALKKLDEAPLVNEIENVWIVGGNAVYKEAMQSDRCHRIYLTEIKETFECDAFFPEITSDFQLVKNDDDVPEDIQEENGIQYQYRIYEKVPK

>DHFR_aedAeg Aedes aegypti (mosquito) AY432005 Diptera; Nematocera exons 2-6 fused
MKKFSLIVAVCANGGIGIKGDLPWRLR 2
1 QELKYFSRMTKKITDTSKRNAIIMGRKTYFGVPESKGPLPERLNIILTRDPSANTYPSDVMVCKSMQEALTKLDESPLANEIEKIWIVGGNAVYKEAMQSERCHRIYLTEIKETYECDAFFPEITSEFQLVKNDDDVPEEIQEENGIQYQYRIYEKLPK

>DHFR_armSub Armigeres subalbatus (mosquito) EU205282 Diptera; Nematocera
MKKFSLIVAVCANGGIGIKGDLPWRLKQELKYFSRMTKKKQDTSKRNAIIMGRKTYFGVPESKRPLPERLNIILTRDPSAHVYPPDVMVCTSMQDALNRLDETPLVDEIENIWIVGGNAVYKEAMQSARCHRIYLTEIKKTFECDAFFPEINSDFQLVKND

>DHFR_danPle Danaus plexippus (monarch) AGBW01009470/AGBW01006850 Lepidoptera
0 MSKVKLNLIAAACENMGIGSNGSLPWRLK 2
1 KEMEYFTTMTTKVKDA SKINAVIMGRRTWDCIPNKNRPLENRLNIVLTRHVDVIKDK 0
1 KEMAYFTTMTTSVSDK KKVNAVIMGRRTWDCIPIKYRPLSNRINIVLTHNVDYVKKQ 0
0 VPKDVVVVSGLDEAVSYIIDRNDVESTWVIGGSSVYQ 0
0 ASMDHPNCGKIYLTEIQKTFDCDTFFPNINRQRFHLVDEDDIPRDIQTEGDISYYFRVYKRL* 0

>DHFR_bomMor Bombyx mori (silkworm) BABH01041437+BABH01041438 Endopterygota; Lepidoptera
0 MSRTQLNLIAAACENMGIGINGTLPWKLK 2
1 KEMAYFTTMTTSVSDK KKVNAVIMGRRTWDCIPIKYRPLSNRINIVLTHNVDYVKKQ 0
0 VPEGVIVVPGLDEAIDHIDSRSDIESTWVIGGSSIYK 0
0 AAMEHPNCGKIYLTEIQKSFDCDTFFPDINKQRFHLVNEEEVPGEKQSEGDINYYYRIYKRL* 0

>DHFR_helVir Heliothis virescens (budworm) AF104106 Lepidoptera
MSQVKLNLIAAACDNMGIGVNGALPWRLKKEMAYFTTMTSKVSEPTKVNAVIMGRRTWDCIPDKYRPLQDRVNIVLTHNVDSVKENVPEGVMVFPGLDEAIKYIEGREDIESTWVIGGSSIYRAAMTHPNCGKIYLTEIQKSFDCDTFFPNIDKQQFHLVDEEQIPGEKQVEGNISYYFRVYKKL

>DHFR_triCas Tribolium castaneum (beetle) AAJJ01000197  Coleoptera exons 2-4 fused
0 MVIKFDLIAAACENMGIGKNNDLPWRLK 2
1 SELAFFSQMTTQTSDESKKNVVLMGRKTWDSIPPKFKPLHQRFNFIMSRAGVNLEGYKDCFSFKSLDEVISKLQDEKFQQLYENVWVIGGSYIYE 0
0 ATMASKYFHRLYLTKVLKTFDCDTFFPKIRDDLIEVRYL 2
1 DPRVPEGVQEENGIQFVYHVYQNPDFNKL* 0

>DHFR_denPon Dendroctonus ponderosae (beetle) GO487443 Coleoptera
 MALKLNLIVAASENWGIGKNGTLPWKLKKEMAFFRTMTSATEDKSKKNVVIMGRRTWESIPAQFKPLPNRINFVLSRSGLNLDQYSNTQSFTNFREALDKLEESAFRQLYENVWIIGGSSLYNETFRSPHFHKLYLTKIHKEFDCDTFLP ALPGNLKEINDPAVTED   IQEENDIEYTFQVFEVIN

>DHFR_apiMel Apis mellifera (bee) HP575683 Hymenoptera
0 MNLHLIAATCEGMGIGIKGTLPWKLK 2
1 SELAFFTYMTTNTKNPNKRNVVLMGRRTWESIPKENRPLKNRINIVLTSQSL 2
1 DYGNDVIVCKNIPHAFEVIEKIKDRIENIWVTGGSSVYK 0
0 EAMESPNFYRLYLTRIKKYFECDTFFPTIPNDFVLTE 2
1 DPNIPQGIQEENDIQFVYEVYKKNRFLIWFQNNIFRIL* 0

>DHFR_bomImp Bombus impatiens (bumblebee) JI114531 Hymenoptera
0 MNLNLIAAACEGMGIGVKGTLPWRLK 2
1 SEMAFFTSMTTNTKDPNKKNVVLMGRRTWECIPKKYKPLKNRINIVLTSQSL 2
1 DYGDDAIVCKSIPHALEVIKKPTLKDQIEGIWVIGGSSVYK 0
0 AAMESPNFHRLYLTRIKKHFVCDTFFPTIPNNFVLID 2
1 DPNIPQGVQEENDIQFVYEVYEKNRFLYLKII

>DHFR_eugCor Euglossa cordata (bee) HP973249 Hymenoptera distal frameshift fixed
MSLNLNIIAAVCEGMGIGVKGSLPWKLKSEMAFFTSMTTQTKDPK KNVVIMGRRTWECIPTKYRPLKDRINVVLTSQNLDYGVNAIICKSMSHALELLKKPPFSDEIESVWVIGGNLVYKAAMESPNFHRLYLTRIKSILTVIHFFPPIPNDFALIQDPNIP

>DHFR_nasVit Nasonia vitripennis (wasp) XM_001605731 Hymenoptera
0 MQVKLKLIAAACENMGIGVNGDLPWRLRK 2
1 EMDFFTKMTSTTKDENKKNVVLMGRRTWESIPKKFKPLSNRINMVLTSQPL 2
1 DLGEDAIVCKNLPEAIEKISQSPLKDKVEQVWVIGGSLVYK 0
0 TSMESPNFYRLYLTRVKKDFKCDAFFPEIPNDYILVK 2
1 DPEVPEGIQEEKDIKFEYEVYEKKQN

>DHFR_copFlo Copidosoma floridanum (wasp) JI834972 Hymenoptera
MQVKLKLIAAACDNMGIGINGDLPWRLRKEMDFFTKMTSTTKDPNKQNVVLMGRKTWESIPKKYKPLANRINVVLSSQPLDLGKDAIACNSLPEAIQQISQDHLRDKVEQVWVIGGSSIYQAAMDSPNFYRLYLTRVKKHFDCDTFFPEIPKDYALVKDPEVPEGIQEEKGINFEYEVYQKA

>DHFR_attCep Atta cephalotes (leafcutter-ant) ADTU01012310  Hymenoptera
0 MPPKLELIAAACENMGIGVNGNLPWRLK 2
1 AEMAYFTRMTTNTRNKNKRNVVLMGRRTWECIPEKYRPLKDRINMVLTSQSL 2
1 NYGDDAIACKSILHALDVISEMQNEVERVWVIGGSSVYK 0
0 SAMESPHFGRLYLTRIKKKFECDTFFPSIPNDLVLIE 2
1 DSTVPQGIQEEKGIEFVYEVYERR* 0

>DHFR_camFlo Camponotus floridanus (carpenter_ant) XP_393902 Hymenoptera 
MPPKLELIAAACENMGIGINGGLPWRLK
TEMAFFTRMTTDTKDKDKKNIVLMGRRTWDCIPEKYKPLENRINMVLTSQSL
NYGDKAIICKSIPHAIEVIAEMQNQVERVWVIGGSRVYK
DVMGSPYFGRLYLTRIKKEFKCDTFFPPIPSGFVLIK
DPTVPQHIQEEKGIKFIYEVYEKK 

>DHFR_harSal Harpegnathos saltator (jumping_ant) XP_393902 Hymenoptera 
MLPKLELIAAACENMGIGIKGDLPWRLK
TEMEYFTRMTTETKDKNKKNVVLMGRRTWDCLPETYRPLRNRINMVLTSQL
MNYGDKAIVCTSIPNALEIITKIQNEVERVWVIGGSKVYK
EAMESPYFGRLYLTRIRKRFECDTFFPPIPNNFVLVQ
DPIVPEDIQEEKGIQFVYEVYEKR

>DHFR_linHum Linepithema humile (argentine_ant) LH21356-RA Hymenoptera
MQLKLELIAAACENMGIGNNGALPWRLK
TEMEYFTRMTIDTKDKNKKNVVLMGRRTWDSIPIKYRPLNDRINMVLTSQSL
DYGDKAIVCKSIPHALEVIAGMQNQIERVWIIGGSSVYK
DAMESPHFGRLYLTRIKKEFECDTFFPPIPNDFVLVT
DPAVPQDVQEEKGIKFVYEVYEKR

>DHFR_pogBar Pogonomyrmex barbatus (harvester_ant) B10193-RA Hymenoptera
MSHKLELIAAACENMGIGINGDLPWRLK
TEMAFFTRMTTETNHTNKKNVVLMGRRTWECIPDKYRPLKDRINMVLTSKSL
DYGNETITCKSISHAFDIITQMQNQIERVWVIGGSSVYK
SAMESPYFGRLYLTRIKKKFECDTFFPPIPNDFVLIE
DPTIPQGIQKENGIEFVYEVYERR 

>DHFR_solInv Solenopsis invicta (fire_ant) AEAQ01011575+AEAQ01011576 Hymenoptera
MPPKLELIAAACENMGIGVNGDLPWRLK
TEMAFFTRMTTDTKQNNKKNVVLMGRRTWECIPKKYRPLKDRINMVLSSQSL
DYKDEAIVCKSIPHALDVISGMQNQVERVWVIGGSSVYK
SAIESPYFGRLYLTRIKKKYECDTFFPLIPNDFVLVE
DPTVPQGIQEENGIEFVYEVYERR

>DHFR_bemTab Bemisia tabaci (whitefly) EZ942456 Hemiptera
MSSLRLSIIVAMTADNGIGFKNSLPWSLPNELRNFAKLTKNCKDKSKQNAVVMGRNTWESIPAQHRPLKDRLNVVLSSTLKDAGEGVLVCPRLKDALTLLEENYQNKIENVWIIGGARVYEDALNLSNCHKLHVTHIQEKPECDVFFPTIPRDFQIITTSEMVEENGFHYNYQVYERRTSPDGEANGIEDSTKS

>DHFR_acyPis Acyrthosiphon pisum (aphid) NM_001162022 Hemiptera one exon
MVYSVIAAVSKNGGIGYKGNLPWKIKKEMEYFNLMTTRVNLKGVQNAVIMGRCTWQSIPDKYRPLKGRINVVISKTLNSVPEGVLLYPKLEEALKSLYLNDRIENLWVIGGSGLYKEAVNDKNCKKLFITKIDQEYLCDTFFPDFDTKKFEETSEANVPKGIQEDNGIKYEFKVFKRL

>DHFR_blaGer Blattella germanica (cockroach) FG125641 Orthopteroidea
MNLKLXLIVAVSENMGIGLNGDLPWRLRKELAHFSRLTKRTIDSNKQNAVIMGRRTWESIPIKNRPLPERLNVVLTKQELDFGKEVLVCNSLNSALEKLCEPRLDKTIESIWVIGGSSVYKEAMSSPHCHRIYLTKIFKTFECDTFLPEIPGNSFKIVRDPDVADEIQEENGLTYEYQVYERILV

>DHFR_pedHum Pediculus humanus (louse) XM_002432121 Phthiraptera
0 MVNNFNLIAAVCSNNGIGYKGNLPWNLR 2
1 KELQYFNRMTKDVKNPEKKNAVIMGRKTWDSLPHNWKPLPGRYNFVLTTQSL 2
1 NLDGAVVCSDLNDLIKKINSPDYSNLIETAWVIGGSKVYESVLKYGLCHRFYLTRIKKEFECDCFFNYDFTKDFKEVS 2
1 DDRVPKGVQKENEIEYVFHVYEKVNESKDSSN* 0

>DHFR_onyArc Onychiurus arcticus (springtail) EW753645 Hexapoda; Collembola
MGPKLYIIAAACENMGIGYKNDLPWKLRNEMAYFNRMTQIVENPGSGSELKNAVIMGRRTWDSIPEKFRPLRNRLNIVLTSRPDFKQPNVLSYPGWYEAVKDLATPEKSLQIDKVWVIGGNSTYKLAVESPDCHRIYLTRVLKHFECDAFFPEFDKSKFRLANDPNVPQDIQEEDGIQYHFEI

>DHFRcalCle Caligus clemensi (sea_louse) BT080999 Crustacea; Maxillopoda; Copepoda
MTSLKCVKINVIAAACKSSRGIGKDNDLPWKLPTDMKYFREKTASTTEIRVGVIMGRRTWESVPPKFRPFKNRFNVVLTSREDFEAKGAVIARTLEDAVKKCSNNVDSIWVIGGSGVYAEALNKYPCRVYLTEIDKEFDCDAFFPPLDEKKYKLASVSPDHQENDITFRFKVYDVIEL

>DHFR_lepSal Lepeophtheirus salmonis (sea_louse) BT121746 Crustacea Copepoda
0 MSKSIPTLHVIAAACRPTNGIGKNNTLPWNLPTDLKYFKTTTSSTPDSKVAVIMGRRTWESIPSKPRPLKNRINVVLTTNSNFVAEGAILA
SSLEEGIKSCMDQADKIWIIGGANLYKEAMQYYKCQIFLTEIDKEFECDTFFPSIDSTKYKEVSVSEDHVENGITFRFKRFERLLQENN

>DHFR_litVan Litopenaeus vannamei (shrimp) FE141783 Crustacea Malacostraca 
MSVRLNIIVAACENHGIGINGELPWKLREEMKYFSRMTKATKSLEKQNAVMMGRKTWESIPAKFRPLPGRLNLVISSRAKDPGPEFNGSTVCASFKEAIDEVQSRTDEVESLWAIGGSSIYE
MALESEHLHRIYLTRILKDFECDTFLPLFDPKKFQIVTD
PAVPAELQQEGDITYKYEIYEKVK

>DHFR_celPug Celuca pugilator (crab) JO488787 Arthropoda; Crustacea
MGPRLNIIVAVAENHGIGKGGELPWRLREEMKHFSRMTKRLDSPATQNVVLMGRKTWESIPLKFRPLPGRLNVVLSSQMKQQQDSSGKVIVCQDFESALKSVSTENPEVETVWVIGGHSVYKMALESEHLHRIYLTKVMKEFECDVFFPSFKMNEFKLVEDPDVPGQV

>DHFR_dapPul Daphnia pulex (water_flea) FE400591 Crustacea Branchiopoda
0 MKLNLIVATASNMGIGFQGTIPWRLK 2
1 KDMALFAKLTKNTNDTNKQNAVVMGRKTWESIPEKNRPLSNRLNIVLSSSQQ 2
1 IENTLTCKSLESAMQLLQTPPYLDQIENIWIIGGASVYK 0
0 EAMEHPSCHRIYVTHILKDFECDVFMPAIDPAKFSLVG 2
1 DSKVPEGTEEENGISFNVKVYEKV* 0

>DHFR_ixoSca Ixodes scapularis (tick) EW930272 Chelicerata Arachnida 3 re-intronated exons
0 MCPGVQESLQYFAIAAMCHNRGIGVLNTLPWRLKKEMAYFSRITSQAAEGKTNAVVMGRNTWDSIPPKYKPLPGR 2
1 VNVVVSRTLEKVPDGHHVARSFSDALSLLQPLVDSGK 0
0 VDKTFVVGGAQLYREALADPRCTRVYLTEIDADFECDVFFPELELGQFRLVQEEGVPQEEQTEGGLTYRFNVYQRISSGSV* 0

>DHFR_ambMac Amblyomma maculatum (tick) JO841362 Chelicerata Arachnida
MCPSHKSVVSCFAIAAMCRNRGIGFKNALPWRLKKEMAFFKRMTSEAAEGKQNAVVMGRNTWESIPPKFRPLNNRINVVVSKTLTEVPEGHHVASSFPAALQLLQTLVDTGKVDKVFLVGGAQLYREALESGHCTRIYLTEVDKDFECDVFFPEFDNSVFSPVEEEGVPQEPQQEDGVTFRFRVYERVQN

>DHFR_varDes Varroa destructor (mite) ADDG01075186 Chelicerata; Arachnida one exon
MAVPPLCAIVAVCKASKGIGNKGSLPWGRSLPNEMKHFARVTTNTTDPDKCNAVVMGRRTWESIPENRRPLPRRINIVISSGLCQESVPEGVKVAKSFDEALEIAGTGCCKGQSRVERIFVIGGTQVYVDAMRHPAMDTVYLTEVLGQFECDTFLNIDTRKFTDIEDPAVTNEEQEENGIRYKYRVLKKIPQ

>DHFR_perSed Peripatopsis sedgwicki (velvetworm) FN242195 Onychophora 
MHRYLNLVVAVCNNLGIGIGGELPWRLRGDMKFFSKLTSETKDSEKTNVVIMGRKTWASIPEKFRPLPKRTNIVISSSLNSVPEGVLIARDFEHAMSLAFGLQQDNIVEQVFVIGGSSVYEQALKSDFPCRIYLTRVMGDFKCDTFFPKLNVDKYALVKVPEIPEEVQEENGISYLFQVLQEKVES

>DHFR_milTar Milnesium tardigradum (tardigrade) EZ761524
--------VAACRNGGIGIRGDLPWTLRGDMRFFTKITSQTKDPQKKNAVIMGRKTWFSIPERFRPLSKRINVVISRTMFGIQPNAIQTQSLEEALDLLSKEPLSSEIETVWIIGGESLYKMAIHSPQLYRVYFTNVMADFECDTFFPTQELQRMKVVSDADVNGDVQMENDVKFQHEIYEP 

>DHFR_triSpi Trichinella spiralis (nematode) ES273161 Nematoda Enoplea
MAQVNIIVAICEKYGIGKKNSLPWHLSKEMQHFKKMTTSVSDPNKINAVIMGRNTWYSIPEKYRPLSGRFNIIISGTMPQLSDEDVIVTWHWREAIEIVEHPISKKAIETFWICGGAKLYNDVIESGLWNRLYVTWIMKEFDCDAFFSFPDKNTIKLVEDDRIPSDIQVEKGIHYKYEVYENCMKMQS

>DHFR_xipInd Xiphinema index (nematode) CV509322 Nematoda Enoplea
MNLIVAACDNMGIGHGNELPWPKLPNESKHFLKLTAGTRDPGKQNAVIMGRKTWEIIPVEHRPLKHRLNVVISRTLSRKDVPPGVQLFEDFEPAMRYLLSPQVSQNIETIWNVGGSEIYALGLDHPSFHRLYFTRVSGTYDCDTYFPQVDWTMLKRVEDPNISTGLHEENGVQYKFETYEHAF

>DHFR_caeEle Caenorhabditis elegans (nematode) NM_059963 Nematoda Chromadorea 4 exons re-intronated
0 MRKMNLIVAMDAEGGIGKNGVLPWRIKKDMQYFASVTKNVSDQSK 0
0 RNAVLMGRKCWESIPVTRRPLAGRLNIVLSRQLPAQKSDDYIVV 2
1 NSLEAAMKLLSEPPFVDSIETIWNIGGAEIYDLALRENLVDEIHLTRIFKN 2
1 FEADVHLKSLDFSKMEKVQNAEVSSENSEIFEENGLKFEFCKWKVVENH* 0

>DHFR_melInc Meloidogyne incognita (nematode) CF803120 Nematoda Chromadorea 3 exons re-intronated
MNIIAAVDENFGIGKNNSLPWRLPKEYKHFINLTTTTKNPNKINAVLMGRKCWESIPEKYRPLKNRLNIVMTKTWVTPEF 0
0 VGENLIFINSLDSLNLILESKPYENLIETIW 2
1 NIGGKQIYSLGIEHQNLNKIVLTKIDKNFDCDVKFPEINWNEFIEEENGEIVEEKGLCWKAITYIKK* 0

>DHFR_ascSuu Ascaris suum (roundworm) JI215038/AEUI01062849/AEUI01051380 Nematoda Chromadorea
0 MVSPKLPINIIVAMDSRGGIGKNGALPWHIPEDLKYFQTMTTKTIDPTKQNAIVMGRKVWESLPAKWRPLKNRLNVVLSNS 0
0 MDDPHDGSYIVTRTFESAIDILNGMSDKIETIWDIGGRRPYEEGLKSSQLRQ 0
0 LYVTFVEGDFDADVFFPDVDFKKFSKQNGDKQSSEHHYEGITYRFETFTALHTDTELS* 0

>DHFR_schMed Schmidtea mediterranea (planaria) DN307116 Platyhelminthes Turbellaria 6 standard exons
0 MKRLNLIVAACENQGIGKNGKLPWNLK 2
1 NEMIYFNNITTSVSDS 1
2 NKQNVVIMGRITWESIPNKFRPMPKRINIVISSTLSR 2
1 DHDNLTGTYLFNNLSEALSFTNDELSDKVEEVFVIGGSSLYK 0
0 AALSSSYPLRLYYTKIFESLDCDTFFPAFEWEQFKSVK 2
1 LDHVPSEVQQENGIRYQFMVLDRC* 0

>DHFR_schMan Schistosoma mansoni (tapeworm) XM_002580511 Platyhelminthes Digenea
0 MRLNVVVAVSENWGIGKGGGLPWKIK 2
1 KDMEFFKTVTTKAHP 1
2 GLKNAVVMGRVTWESIPESFKPLKDRINIVVSSTLSH 2
1 APSFVQVVPSLNAAIDLLYNEEFSSIVDEVFIIGGYRLYK 0
0 EALKQS YPVRIYCTHILSEVDCDTYFPKVDWDKLKK 2
1 VDLPDIPADTFTENGFTFKFCVYDVPSEQFI

>DHFR_taeSol Taenia solium (tapeworm) PEL761437 Platyhelminthes Cestoda
MGLRRLNVIAAVAKNGGIGKENKLPWHIREDMAFFSRITSTAQEGKKNAVVLGRRTWLSFPPKFRPLPNRVNVLVSTQLESVPEGAYLVKSFEDSLRLVESLVDGGEVDEVFIIGGHGLYKEALEQEVYPVRLYYTHIMKDFDCDTFFPSVDWERFTPIQLDTVDSNPKYCGEIEFRFAVYEKGPHRFHDN

>DHFR_aplCal Aplysia californica (sea_hare) AASC02008512 Mollusca Gastropoda one exon
MTSTKLNIVVAVCTNMGIGIEGRLPWRLKQDMAFFKQLTVETQDEQKKNMVIMGKKTWMSIPTKFRPLQDRVNVVLSTQLTEPPSGALLASSLKEAVNLAKSDANVENVFVIGGASVYREAVEGDCPCRIYLTRVDKEFECDTFFPKFDTDVFERIQNPNNVPTGINVEGDLSFTFEVYEKNLFPH

>DHFR_lotGig Lottia gigantea (limpet) FC707856 Mollusca Gastropoda one exon 
MCTKRLNIVVAACKNNGIGVNGSIPWRLKKDMAMFRHITSDTVDESKQNAVIMGRKTWMSIPDKFRPLKNRVNIILSNTLSESPDGTYIVKNFQQALSLVNSDKMKQQIESVHIIGGSSVYKEAMESDYDCRVYLTKVDADFECDTFLPPIEESLFQRVKNPSNIPSDVQEENGIQFTYEIYDKTTDQSDRESPVSR

>DHFR_phyAcu Physella acuta (snail) BW985974 Mollusca Gastropoda 
MPKLNIIVAACNNNGIGIEGRLPWRLKSDMSFFKQITLRTQDAEKKNAVIMGKNTWFSIPSKFRPLVGRINVVLSTQMTDAPEGTQLAKSLNQAVSLLESLNVENIFVIGGSTVYKQAMEGEHPCRIFLTRIAADFLRDTFLPDIPN

>DHFR_pinMax Pinctada maxima (oyster) GT283724 Mollusca Bivalvia
MSKCKLNLVVAACNNRGIGIDGQLPWRLRKDMDFFKKITTETNDPEKRNAVIMGRKTWFSIPEKFRPLSKRINIILSREMKEVPENTYVARSLDEAVQLVSDGGELADRVESIHIIGGSSVYKEALASSHPCRIYFTRVLADFKCDTFLPEFDENKYQKLENMENIPTEIMTEKDINF

>DHFR_mytCal Mytilus californianus (mussel) ES395435 Mollusca Bivalvia
MSKTKSTLNLVVAACNSRGIGINGKLPWRIRKDMDFFKKITMETKNPDKKNVVIMGRKTWFSIPEKFRPLPKRINIILSREMKEAPPGVYIARSFDDAITMVNKDLSDKAESIYVIGGSSIYKAAMDSDYDIRIFLTKILADYDCDTFLPQFDENLYKIIENYEGVPKGKQTENDIDFIFEVYEKVR

>DHFR_dreRos Dreissena rostriformis (quagga_mussel) EY433849 Mollusca Bivalvia
MSMPRLNLVVAICNNNGIGINGSLPWKLRKDMDFFKTITMTTTDPEKQNAVIMGRKTWQSIPENFRPLRNRINIILSRDMNAPNGTHVARSLPDALSLLSSEELADRVEQIHIIGGSSVYKEAMTGPHPCRLYLTRILADFECDAFLPEIDDSSFTKIRNPDNVPTDLQEENGLKFRFEVYDKL

>DHFR_alvPom Alvinella pompejana (bristleworm) GO125274 Annelida Polychaeta 
MPPTLNLIVAMCNNNGIGIQGKLPWKIK
GDMAFFRKMTTETKNPAKMNAVIMGRKTWFSIPEKNRPLNKRMNIILSQTMSSVPDGVHLARSFSEALNLISTKPLADIIEGIFVIGGASVYKEALESSYPCRIYLTRVFADVPCDTFMPTFSGFKQIECAEIDNTVQKENDWQYKFEVYEKV

>DHFR_helRob Helobdella robusta (leech) EY386022 Annelida Clitellata six exons
0 MQPKLQIVVALCVKNRGIGLNNSIPWKLP 2
1 GDMTFFRKLTSETSIL 1
2 GSKNAILMGRKTWDSIPSNLKPLKNRLNVVISRTLE 2
1 cPDInGRLHVSKSFEDAVEFVASDKSIDKIFIIGGSSIYD 0
0 LALASSTCYNVFITEISNDFQCDTFFPKFNQDAYKLIK 2
1 YPGHSTSVQFENGIGYQFTCHHKVSK* 0

Cnidarians, ctenophores, trichoplax, sponge and choanoflagellate reference sequences

>DHFR_nemVec Nematostella vectensis (anemone) ABAV01013911 Cnidaria six exons
MAAVGVRFSCIVAMDLKRGIGKNNDLPWKIK
GDMKFFTEKTSEVKTE
GKQNAVIMGRKVWESIPEKFRPLKGRLNIVLSRTLS
EPPQGAQLCRSFEQALTILSTDPYTKKIENVFVCGGSALYK
DAMAHSACTRIYITYIDQEFDCDVFFPEFDQNTYHLVE
DPDVPSVQHEEKGIKYKFCVYDRCQ

>DHFR_acrMil Acropora millepora (coral) EZ020360 GO002175 Cnidaria Anthozoa Hexacorallia 
VAVADNGGIGKENRLPW HLK
GDMKFFSHLTSTVATE
GKQNAVIMGRNTWESIPKKYRPLPRRLNIVLSRKMS
EPPEGAVLCSSLQDALDRLRSAPYTDTVEKVFVIGGATVYE
EAMQHHQCYRLYITHIYKDFECDVKFPEFDKRIYKETR
DIDVSSEMLEEKGIPYEFKVYQKD

>DHFR_hydMag Hydra magnipapillata (hydra) XM_002157178 Cnidaria Hydrozoa one exon
MVQLRKVHCIAAIDSEWGIGLKGKLPWNLPREYKFFQNITTKVISEGKQNAVIMGKNTWFSIPQQHRPLKNRLNVILSSSFIKEDYPKNVLLESSLEAAILRLSDEFYANTVENIFVIGGSRVYKEAMEKFCDKIYLTKIEQDYSCDVFYPIFDTNKFKEIDDEEVDKNKQIENGVSYTFHVYSSNYTV

>DHFR_mneLei Mnemiopsis leidyi (sea_walnut) AGCP01012173 Ctenophora exons 2-3 fused
0 MKLRFNIIVATDLEGGIGKNNNLPWRCR 2
1 KEFRHFTDTTSKLQNGKQGAAVMGRKTWESIPEKFRPLKNRHNFVISSTLS 2
1 VANPGNVPVYKTISDCMTALDQDKD IGSVWIVGGWGIYK 0
0 EAMESERLHRMYITTVQGKFDCEVFFPSFDERNFNLVI 2
1 DPDIPNETVTEDNGISWSVSVYERK

>DHFR_triAdh Trichoplax adhaerens (placozoa) ABGP01000098 same six exons
0 MQKNFRMIAAMTRDRGIGYKNDLPWKLR 2
1 NEYKYFARLTTSTKDS 1
2 AKKNAVLMGRNTWVSIPEKNRPLRNRINIVISSQLR 2
1 AEEVPEGVHVVASIPEVIELVQSPLLKDIVETVWIVGGAPVYK 0
0 GFMSHPQCDRIYLTKLDANYECDVFFPEMSDDFKEIS 2
1 DPDVSQEVQEENGLKYTFHVYQRN* 0

>DHFR_subDom Suberites domuncula (sponge) GH558547 Porifera Demospongiae
MYIMVAKEIKSGIGNKGKIPWPRINTDMNFLETMTKKTRNKTKQNAAIMGRLTWESIPAKYRPLPERFNIVLSSKKFEDIAPGAHMVCSSLSFAMEKLRAPPLSDQIENVWIFGGAGVYEESMKHPDCDKLYVTEVLQEFPCDAFCPPIDSDIYKLIDHSDDPDVPKGEFVENGVKFKFEVYQRQ

>DHFR_monBre Monosiga brevicollis (choanoflagellate) ABFJ01001321
0 MRVALVVAATKAGGIGHQGQLPWHLK 2
1 ADMQHFRDITTKTEQP 1
2 AAINAVIMGRKTFESIPDKFRPLHGRLNVVLSTQKP 2
1 VSLPLHTPHDTNPPQPLPHPACLAHPQPEVIETAYIIGGASVYN 0
0 EALAKPEYSHYIFMTKVHKDVLCDTVMDMKHLDAYEHDPNFPVq 2
1 * 0