Finding nearby genes: Difference between revisions

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(Adding other script examples for other gene sets/assemblies)
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* [http://main.g2.bx.psu.edu/ Galaxy] has a "Fetch closest non-overlapping feature" tool
* [http://main.g2.bx.psu.edu/ Galaxy] has a "Fetch closest non-overlapping feature" tool
* the BedTools include a tool "closestBed"
* the BedTools include a tool "closestBed"
 
===Script for knownGene on hg18===
<PRE>
<PRE>
#!/bin/sh
#!/bin/sh
Line 51: Line 51:
+------+--------+--------+------------+----------+
+------+--------+--------+------------+----------+
</PRE>
</PRE>
===Script for ncbiRefSeq on hg38===
Here is a script for the gene set ncbiRefSeq on hg38:
<PRE>
#!/bin/sh
# for gene set ncbiRefSeq
# given position chr1:991973-991973
# find a sample of genes near this upstream and downstream
# Input your assembly
G=hg38
# Input the chr for reference point
C=chr1
# Input start for reference point
S=991973
# Input end for reference point
E=991973
# Input the number of nearby transcripts to output
N=10
# Any gene set can be used. If a different gene set is used, check that
# the field names are the same, they may need updating. To check this,
# go to the Table Browser, select your gene set, and click the link for
# "table schema" to see field names. Older assemblies may use the related
# kgXref table for gene alias/gene name.
# The last column is the distance from the comparison point.
echo "closest upstream transcripts from ${C}:${S}-${E} in ${G} for ncbiRefSeq set"
echo "last column is distance from reference point to transcript, ${S} - txEnd"
echo "Note: for reverse - strand items, txEnd is the 5' end, the transcription \
start site"
mysql --user=genome --host=genome-mysql.soe.ucsc.edu -A -e \
'select e.chrom,e.txStart,e.txEnd,e.strand,e.name,j.name,"'${S}'" - e.txEnd
AS "'${S}'-txEnd" FROM
  ncbiRefSeq e,
  ncbiRefSeqLink j
WHERE e.name = j.id AND e.chrom="'${C}'" AND e.txEnd < "'${S}'"
ORDER BY e.txEnd DESC limit '${N}';' $G
echo "closest upstream transcripts from ${C}:${S}-${E} in ${G} for ncbiRefSeq set"
echo "last column is distance from reference point to transcript, ${E} - txEnd"
echo "Note: for reverse - strand items, txStart is the 3' end, not the transcription \
start site"
mysql --user=genome --host=genome-mysql.soe.ucsc.edu -A -e \
'select e.chrom,e.txStart,e.txEnd,e.strand,e.name,j.name,"'${E}'" - e.txStart
AS "'${E}'-txStart" FROM
  ncbiRefSeq e,
  ncbiRefSeqLink j
WHERE e.name = j.id AND e.chrom="'${C}'" AND e.txStart > '${E}'
ORDER BY e.txStart ASC limit '${N}';' $G
</PRE>
Which gives the output:
</PRE>
closest upstream transcripts from chr1:991973-991973 in hg38 for ncbiRefSeq set
last column is distance from reference point to transcript, 991973 - txEnd
Note: for reverse - strand items, txEnd is the 5' end, the transcription start site
+-------+---------+--------+--------+----------------+---------+--------------+
| chrom | txStart | txEnd  | strand | name          | name    | 991973-txEnd |
+-------+---------+--------+--------+----------------+---------+--------------+
| chr1  |  975198 | 982117 | -      | NM_001291367.1 | PERM1  |        9856 |
| chr1  |  975198 | 982117 | -      | NM_001291366.1 | PERM1  |        9856 |
| chr1  |  975198 | 982093 | -      | XM_017002583.1 | PERM1  |        9880 |
| chr1  |  975198 | 982021 | -      | XM_017002584.1 | PERM1  |        9952 |
| chr1  |  975197 | 981657 | -      | XM_017002585.1 | PERM1  |        10316 |
| chr1  |  966496 | 975108 | +      | NM_032129.2    | PLEKHN1 |        16865 |
| chr1  |  966496 | 975108 | +      | NM_001160184.1 | PLEKHN1 |        16865 |
| chr1  |  965819 | 974587 | +      | XM_006710944.3 | PLEKHN1 |        17386 |
| chr1  |  965819 | 974587 | +      | XM_017002476.1 | PLEKHN1 |        17386 |
| chr1  |  965819 | 974587 | +      | XM_017002474.1 | PLEKHN1 |        17386 |
+-------+---------+--------+--------+----------------+---------+--------------+
closest upstream transcripts from chr1:991973-991973 in hg38 for ncbiRefSeq set
last column is distance from reference point to transcript, 991973 - txEnd
Note: for reverse - strand items, txStart is the 3' end, not the transcription start site
+-------+---------+---------+--------+----------------+--------------+----------------+
| chrom | txStart | txEnd  | strand | name          | name        | 991973-txStart |
+-------+---------+---------+--------+----------------+--------------+----------------+
| chr1  |  998961 | 1000172 | -      | NM_021170.3    | HES4        |          -6988 |
| chr1  |  998961 | 1001052 | -      | XM_005244771.4 | HES4        |          -6988 |
| chr1  |  998963 | 1000172 | -      | NM_001142467.1 | HES4        |          -6990 |
| chr1  | 1013466 | 1014540 | +      | NM_005101.3    | ISG15        |        -21493 |
| chr1  | 1020101 | 1056119 | +      | XM_011541429.2 | AGRN        |        -28128 |
| chr1  | 1020101 | 1056119 | +      | XR_946650.2    | AGRN        |        -28128 |
| chr1  | 1020101 | 1056119 | +      | XM_005244749.3 | AGRN        |        -28128 |
| chr1  | 1020122 | 1056119 | +      | NM_198576.3    | AGRN        |        -28149 |
| chr1  | 1020122 | 1056119 | +      | NM_001305275.1 | AGRN        |        -28149 |
| chr1  | 1059706 | 1066441 | +      | XR_001737601.1 | LOC100288175 |        -67733 |
+-------+---------+---------+--------+----------------+--------------+----------------+
</PRE>
===Script for ncbiRefSeq on hg38===
Here is a script for the gene set refGene on hg19:


[[Category:Technical FAQ]]
[[Category:Technical FAQ]]
[[Category:User Developed Scripts]]
[[Category:User Developed Scripts]]

Revision as of 18:52, 26 May 2017

Let's say you had a position, and you wanted to find a sample of nearby genes upstream and downstream from this position.


This can be done with a MySQL query to the public MySQL server

Alternatives:

  • Galaxy has a "Fetch closest non-overlapping feature" tool
  • the BedTools include a tool "closestBed"

Script for knownGene on hg18

#!/bin/sh

# given position chr1:710000-720000
# find a sample of genes near this upstream and downstream
C=chr1
S=710000
E=720000

echo "three upstream genes from ${C}:${S}-${E}"
mysql --user=genome --host=genome-mysql.soe.ucsc.edu -A -N -e \
'select e.chrom,e.txStart,e.txEnd,e.alignID,j.geneSymbol FROM
   knownGene e,
   kgXref j
WHERE e.alignID = j.kgID AND e.chrom="'${C}'" AND e.txEnd < '${S}'
ORDER BY e.txEnd DESC limit 3;' hg18

echo "three downstream genes from ${C}:${S}-${E}"
mysql --user=genome --host=genome-mysql.soe.ucsc.edu -A -N -e \
'select e.chrom,e.txStart,e.txEnd,e.alignID,j.geneSymbol FROM
   knownGene e,
   kgXref j
WHERE e.alignID = j.kgID AND e.chrom="'${C}'" AND e.txStart > '${E}'
ORDER BY e.txStart ASC limit 3;' hg18

This produces the output:

three upstream genes from chr1:710000-720000
+------+--------+--------+------------+----------+
| chr1 | 690107 | 703869 | uc001abo.1 | BC006361 |
| chr1 | 665195 | 665226 | uc001abn.1 | DQ599872 |
| chr1 | 665086 | 665147 | uc001abm.1 | DQ600587 |
+------+--------+--------+------------+----------+
three downstream genes from chr1:710000-720000
+------+--------+--------+------------+----------+
| chr1 | 752926 | 778860 | uc001abp.1 | BC102012 |
| chr1 | 752926 | 778860 | uc001abq.1 | BC042880 |
| chr1 | 752926 | 779603 | uc001abr.1 | CR601056 |
+------+--------+--------+------------+----------+

Script for ncbiRefSeq on hg38

Here is a script for the gene set ncbiRefSeq on hg38:

#!/bin/sh

# for gene set ncbiRefSeq
# given position chr1:991973-991973
# find a sample of genes near this upstream and downstream

# Input your assembly
G=hg38
# Input the chr for reference point
C=chr1
# Input start for reference point
S=991973
# Input end for reference point
E=991973
# Input the number of nearby transcripts to output
N=10

# Any gene set can be used. If a different gene set is used, check that
# the field names are the same, they may need updating. To check this,
# go to the Table Browser, select your gene set, and click the link for
# "table schema" to see field names. Older assemblies may use the related
# kgXref table for gene alias/gene name.
# The last column is the distance from the comparison point.


echo "closest upstream transcripts from ${C}:${S}-${E} in ${G} for ncbiRefSeq set"
echo "last column is distance from reference point to transcript, ${S} - txEnd"
echo "Note: for reverse - strand items, txEnd is the 5' end, the transcription \
start site"
mysql --user=genome --host=genome-mysql.soe.ucsc.edu -A -e \
'select e.chrom,e.txStart,e.txEnd,e.strand,e.name,j.name,"'${S}'" - e.txEnd
AS "'${S}'-txEnd" FROM
   ncbiRefSeq e,
   ncbiRefSeqLink j
WHERE e.name = j.id AND e.chrom="'${C}'" AND e.txEnd < "'${S}'"
ORDER BY e.txEnd DESC limit '${N}';' $G


echo "closest upstream transcripts from ${C}:${S}-${E} in ${G} for ncbiRefSeq set"
echo "last column is distance from reference point to transcript, ${E} - txEnd"
echo "Note: for reverse - strand items, txStart is the 3' end, not the transcription \
start site"
mysql --user=genome --host=genome-mysql.soe.ucsc.edu -A -e \
'select e.chrom,e.txStart,e.txEnd,e.strand,e.name,j.name,"'${E}'" - e.txStart
AS "'${E}'-txStart" FROM
   ncbiRefSeq e,
   ncbiRefSeqLink j
WHERE e.name = j.id AND e.chrom="'${C}'" AND e.txStart > '${E}'
ORDER BY e.txStart ASC limit '${N}';' $G

Which gives the output:

closest upstream transcripts from chr1:991973-991973 in hg38 for ncbiRefSeq set last column is distance from reference point to transcript, 991973 - txEnd Note: for reverse - strand items, txEnd is the 5' end, the transcription start site +-------+---------+--------+--------+----------------+---------+--------------+ | chrom | txStart | txEnd | strand | name | name | 991973-txEnd | +-------+---------+--------+--------+----------------+---------+--------------+ | chr1 | 975198 | 982117 | - | NM_001291367.1 | PERM1 | 9856 | | chr1 | 975198 | 982117 | - | NM_001291366.1 | PERM1 | 9856 | | chr1 | 975198 | 982093 | - | XM_017002583.1 | PERM1 | 9880 | | chr1 | 975198 | 982021 | - | XM_017002584.1 | PERM1 | 9952 | | chr1 | 975197 | 981657 | - | XM_017002585.1 | PERM1 | 10316 | | chr1 | 966496 | 975108 | + | NM_032129.2 | PLEKHN1 | 16865 | | chr1 | 966496 | 975108 | + | NM_001160184.1 | PLEKHN1 | 16865 | | chr1 | 965819 | 974587 | + | XM_006710944.3 | PLEKHN1 | 17386 | | chr1 | 965819 | 974587 | + | XM_017002476.1 | PLEKHN1 | 17386 | | chr1 | 965819 | 974587 | + | XM_017002474.1 | PLEKHN1 | 17386 | +-------+---------+--------+--------+----------------+---------+--------------+ closest upstream transcripts from chr1:991973-991973 in hg38 for ncbiRefSeq set last column is distance from reference point to transcript, 991973 - txEnd Note: for reverse - strand items, txStart is the 3' end, not the transcription start site +-------+---------+---------+--------+----------------+--------------+----------------+ | chrom | txStart | txEnd | strand | name | name | 991973-txStart | +-------+---------+---------+--------+----------------+--------------+----------------+ | chr1 | 998961 | 1000172 | - | NM_021170.3 | HES4 | -6988 | | chr1 | 998961 | 1001052 | - | XM_005244771.4 | HES4 | -6988 | | chr1 | 998963 | 1000172 | - | NM_001142467.1 | HES4 | -6990 | | chr1 | 1013466 | 1014540 | + | NM_005101.3 | ISG15 | -21493 | | chr1 | 1020101 | 1056119 | + | XM_011541429.2 | AGRN | -28128 | | chr1 | 1020101 | 1056119 | + | XR_946650.2 | AGRN | -28128 | | chr1 | 1020101 | 1056119 | + | XM_005244749.3 | AGRN | -28128 | | chr1 | 1020122 | 1056119 | + | NM_198576.3 | AGRN | -28149 | | chr1 | 1020122 | 1056119 | + | NM_001305275.1 | AGRN | -28149 | | chr1 | 1059706 | 1066441 | + | XR_001737601.1 | LOC100288175 | -67733 | +-------+---------+---------+--------+----------------+--------------+----------------+

Script for ncbiRefSeq on hg38

Here is a script for the gene set refGene on hg19: