Opsin evolution: Difference between revisions

Revision as of 21:33, 17 November 2007

Below is a large set of phylogenetically representative hand-curated opsin sequences that serves as a gene family classifier ... just blast an unknown sequence against the database below (using http://www.proweb.org/proweb/Tools/WU-blast.html) and look for consistent labelling of the top hits.

The set of sequences is not intended to be exhaustive. Rather, if a given clade has many available similar sequences, those with genome assemblies are chosen to represent the group, for example anole is preferred to gecko, and (rightly or wrongly) any experimental results transfered over. This avoids uninformative clutter from near-identical sequences. If the clade reflects a very deep divergence such as lamprey or amphioxus, all available sequences are provided so as to break up long branches. About half the sequences are not available from GenBank but rather are culled from trace archives, genomic contigs, and genome assemblies, typically by blastx against the full (and growing) set of reference sequences. The level of error is very low, declines with time as anomalies are revisited and fixed, but never reaches zero because of problems inherent to experimental data, incomplete assemblies, and sequence manipulation.

The fasta header of each sequence is a miniature database, with fields showing the opsin type, genus, species and common name, accession number or other source, indels, introns, sequence length, lambda max adsorption, and G protein type with which it interacts. These new standardized fasta headers also serve to summarize the collection.

The protein sequences are broken into their constituent exons using genomic information when available. When not available (eg the opsin originated as a cDNA in a species lacking a genome project), the exons are inferred from the phylogenetically closest opsins. The numbers flanking exons, 012, show the phasing of each intron, eg 12 means an overhang of 1 bp at the 3' end of an exon with that fragmentary codon completed by a 2 bp overhang at the beginning of the next exon. Intron position and phasing are generally conserved over great evolutionary distances -- note here lamprey eel has identical intronation of its opsin genes orthologous to human. Cone and rod opsin paralogs are intronated identically in all species with the exception of LWS opsins which have an extra early intron of phase 12. LWS must have acquired this prior to divergence of lamprey.

Syntentic relationships are also shown. The nearest flanking HUGO-named genes are first chosen for the human opsin, two on each side. The strand orientation noted relative to a fixed convention of plus strand for the human opsin. Then each assembly is revisited to determine the extent of conservation of these flanking genes. In the event humans lack the gene, synteny is defined by the nearest diverging species, typically platypus, that has the gene. Sometimes the original synteny is only partly retained (left- or right-synteny). For deeply diverging species such as amphioxus with an assembly, flanking genes there are pushed forward into other species to help define orthologous opsins (blast clustering can be uncertain because of the diminishing percent identity).

Melanopsins, the unexpected rhabdomeric-class Gq-coupled opsin recently found in upper deuterostomes, are readily confused homologically due to various expansions and contractions. Mammals, human through platypus, have a single melanopsin. However chicken, lizard, frog, and teleost fish experienced a multi-gene segmental duplication and the resulting melanopsins were both retained (though diverged substantially). In ray-finned fish, a processed retrogene arose that may be functional in zebrafish though lost in fugu and stickleback. After its whole genome duplication, zebrafish also retained two copies of the original melanopsin. Chondrichthyes also have a second copy of the primary melanopsin but synteny -- which is essential for analysis since intron placement is uninformative in duplications and sequence alignment is too dependent on unknown rates -- is not available in the current contig-level assembly.

Amphioxus also contains two melanopsins from an apparently independent duplication. Flanking gene order today bears no relation to vertebrate gene order. The lamprey situation awaits assembly of its traces or targeted transcript studies. At this time, only a four exon fragmentary melanopsin can be recovered (however with high percent identity, 80%). Possibly orthologs of this melanopsin locus could be tracked into the highly derived tunicates, acorn worm, and sea urchins. The distinctive intron pattern may even allow melanopsin antecedents to be identified in Cnidaria and Protostomia. At this point, the best blastp match to insects stands at 37% with no evident syntenic or intronic support

While clade-specific proliferation of melanopsins -- and implied role subfunctionalization -- confounds the situation for chordates, it really has little impact on the opsin classifier described here. Unknown sequences will readily find their place because of excellent phylogenetic distribution of reference sequences and the inherent distance of melanopsins from the ciliary collection. The main utility at the level of opsin classifier is the ability to identify other rhabdomeric opsins in later deuterostomes should they occur. At the level of alignment, the melanopsis serve as outgroup to ciliary opsins and so help define motifs specific to Gt-coupled signaling and other structure/function issues.

Please do not add or edit sequences at this time -- email me with suggestions. tom @ cyber-dyne. com. After finishing the encephalopsins upgrade, I will update the alignments, fasta headers, rare genomic event sectors (indels and introns), provide some ancestral sequences at the common ancestor to lamprey, and provide a definitive gene tree. Some sections, such as vision in the ancestral mammal will be split off into separate articles.

A dozen very recent publications have shaken our understanding of the evolution of light reception capabilities. After reviewing topics such as ciliary opsin in protostomes, rhabdomeric opsins in deuterostomes, rich opsin repertoires in cnidarians, and other novel opsin classes, I will consider topics such as the origin of image-forming eyes beween amphioxus and lamprey divergences, noting however that our notion of 'eye' is much more nuanced today. The reconstruction of the ur-bilateran eye probably awaits additional cnidarian genomes -- no new ones are being undertaken unfortunately. However the plethora of new arthopod and lophotrochozoan genome assemblies has opened up new avenues of research as the realization grows that fly and nematode are exceedingly derived, with better ancestral characters retained in other species.

Numerous conflicting gene trees have been published for ciliary opsins. Some methodologies have bordered on the preposterous -- thin phylogenetic coverage, dimly related outgroups such as drosophila rhabdomeric opsin, and naive fixed underlying mutational models assumed for maximal likelihood software despite the great diversity of species and many billions of years of branch length. Nonetheless, the resultant trees have only moderate conflict, suggesting that a definitive opsin tree might not be far off.

Rare genomic changes have lately come into vogue as a supplement to traditional maximal likelihood methods, primarily to resolve polytomies (divergence nodes tightly spaced close in time) and otherwise uncertain gene or species tree topologies. The rare genomic changes applicable to opsins include coding indels (deletions and insertions), intron placement (position and phase comparison), synteny (gene order along the chromosome), and gene copy number change (gene gain from retropositional, tandem, segmental, and whole genome duplications; gene loss from pseudogenization or deletion). Results from these methods must be evaluated for their susceptibility to homoplasy (misleading recurrent independent events that mimic a single event) and incomplete penetration in the population level at the time of speciation (lineage sorting).

Among other phylogenetically informative rare genomic events, we'll be looking at a 6 bp amino acid insert, a novel 12 upstream intron in LWS, and post-GWSR introns in rod/cone opsins, all events located between transmembrane helices TM2 and TM3, ie in extracellular loop 2. Their lack of homoplasy can be seen in the massive alignments below.

Because not all cDNA sequences takes place in species having genome projects and not all species having genome projects have cDNAs, existing cDNAs had to be aligned within the heterologous genome project in order to determine their intron placement. As an example, lamprey opsins from Geotria australis and Lethenteron japonicum worked as queries to locate orthologs within the Petromyzon maritimus genome project (which consists solely of 19 million traces as of mid-November 2007).

The first point to be understood in ciliary opsin evolution is jawless fish such as lamprey exhibit a full-blown set of modern rod and cone opsins whereas early deuterostomes such as hemichordates, echinoderms, amphioxus and tunicates genomes totally lack them (Xenoturbella is not available yet) and indeed altogether lack conventional imaging eyes while using protostome-like rhabdomeric opsins with their disjunct signaling system for photorecepton. Of course, characters in extant (living) species should never be confused with ancestral characters at the time of divergence nodes (last common ancestors); conceivably these early diverging deuterostomes have lost opsin genes, perhaps due to a habitat shift to deep water or burrowing habitat.

However the molecular evidence is quite clear that full-blown pentachromatic color vision and most other modern ciliary opsin classes first appeared during the evolutionary stem preceding lamprey divergence. The oldest known fossil lamprey, Priscomyzon, dates at 360 myr to the Devonian. Molecular clocks place lamprey appearance at approximately 430 myr, some 100 million years after Chengjiang and Burgess Shales fossil Lagerstatte formed. Like most soft tissues, eyes seldom leave a good fossil record, though bilateral placement might be reflected in bone orbits.

Hagfish, sister group to lamprey, have imaging eyes but have not been studied; their opsins situation may be derived due to deepwater marine habitat (similarly deepwater coelocanth opsins are adapted to 420 nm). The next-diverging chondrichtyes have inadequate data at GenBank -- only a few rhodopsin genes from skates and dogfish.

This makes even fragments from the partially sequenced elephantfish Callorhyncus milii quite valuable. Those 9 fragments and 3 from the lamprey genome are provided in the data section. The opsin classifier tool can reliably type a fragment from a single mid-sized exon. While full length genes are always preferable, these fragments serve to prove existence of that gene class at the time of a given divergence node. Further, they can validate certain rare genomic events provided the fragment happens to overlap the region of interest.

Despite 6 sequenced opsin mRNAs in the amphioxus Branchiostoma belcheri and an initial assembly in Branchiostoma floridae, no rod/cone opsin can be located there or in earlier diverging deuterostomes with genome projects (3 unicates, 2 urchins, 1 acorn worm). These species may have larval eye spots, ocelli, pigment cells, and related photoreceptors but lack imaging eyes.

The fossil record is unsatisfactory: less than 1 bilateran in 10,000 in Chengjiang and Burgess Shale fossils is even a candidate for deuterostomy. Low numbers of specimens and poor preservation conspire with career pressure and cite-seeking journals to egregiously misinterpret data in the analysis of Hou, discoverer of the Chinese lagerstaette. Myllokunmingia is in the best situation with 500 specimens but Haikouichthys as stem deuterostome, Metaspreggina as post-ediacaran, and Yunnanozoan are all problematic (in the eye of the beholder). While signs of bilaterily disposed eyes are sometimes inferred, it does not follow these were image-forming eyes. Indeed contemporary Branchiostoma and tunicate larva have an eye-spot (ocellus); the genomes contain ciliary opsins clustering to approximately ENCEPH and PPIN -- still a long long road to imaging opsins. Echinoderms and hemichordates genomes have opsins but even more remote. Sea urchin genome encodes at least six opsins, four of these cluster classify to rhabdomeric, ciliary and Go-type. Tube feet are apparently the photosensory organ in adult urchins.

Meanwhile, thousands of high-quality Cambrian arthropod fossils unmistakably show stalked paired eyes. Fossil trilobite eyes are much studied, due to use of calcite as lens crystalin. Imaging eyes of contemporary arthropods and lophotrochozoa are rhabdomeric, utilizing depolarizing Gq-type receptor, phospholipase C, phosphoinositola, diacylglycerol, and transient receptor potential TRP and TRPL channel signaling. However their genomes can also contain ciliary opsins, using hyperpolarizing Gt-type transducins and phosphodiesterase cGMP second-messaging (as well as Go-type gustducin ciliary opsins in other types of photoreceptors).

Vertebrates are just the opposite, having crossed over to a ciliary opsin-based imaging system, while retaining rhabdomeric signaling in melanopsin retinal ganglion cells. Cnidarian opsins are available from Hydra and Nematostella genomes. Hydra expresses a ciliary-type opsin in ectodermal sensory nerve cells whereas Nematostella has opsins classifying between melanopsin and encephalopsin.

It must not be thought that bilaterans invented imaging eyes because earlier diverging cubomedusan jellyfish Carybdea marsupialis has 4 eyestalks each with 6 photoreceptors of 4 types: simple eyespots, pigment cups, complex pigment cups with lenses, and camera-type eyes with a cornea, lens, and retina. This jellyfish tracks, captures, and eats teleost fish. The species very much needs a genome project.

Thus there is no evidence whatsoever -- and every reason to doubt from genomic analysis -- that deuterostomes had imaging eyes during the Cambrian. Despite this, a BBC series, Walking With Monsters, portrayed a school of 25 mm Haikouichthys attacking and wounding an Anomalocaris twenty times their size. It is easy to guess at the scientific advisory panel. This recurrent anthropocentric theme is echoed by fantastic museum imagery of early mammals nimbly predating on dinosaur nests -- dioramas quietly dismantled after Yucatan meteriorite discovery.

Imaging eyes are not essential to survival; even today subterranean mammals such as blind mole rat flourish without them. Discounting ray-finned fish numbers, a very substantial proportion of extant animal species lack imaging eyes 525 myr after the Cambrian. Of 33 animal phyla, a one-third have no specialized organ for detecting light, one-third have light-sensitive organs, and the remaining 6 have imaging eyes (Cnidaria, Mollusca, Annelida, Onychophora, Arthropoda, and Chordata). Thus 82% of animal phyla have survived well over 500 myr without imaging eyes despite the supposedly unrelenting competition/predation from animals with them.

The table below is a tab-delimited database that can be pasted into excel for further analysis. For example, sorting on the second column would show all the opsin sequences available for a fixed species. Sorting on the lambda max absorption column would group the data by color property (when that column is better filled in). The columns show gene name, genus, species, common name, heterotrimeric G protein alpha subunit used in signaling, intron structure, synteny (2 flanking genes on each side of the opsin), indel status, sequence length, lambda max, and comment field.

1	>RHO1_homSap	Homo	sapiens	(human)	Gt	0...2.1.0.0	-MBD4	+IFT122	+H1FOO	-PLXND1	indel	349 aa	497 nm	
2	>RHO1_monDom	Monodelphis	domesticus	(opossum)	Gt	0...2.1.0.0	-MBD4	+IFT122	+H1FOO	-PLXND1	indel	349 aa	000 nm	
3	>RHO1_ornAna	Ornithorhynchus	anatinus	(platypus)	Gt	0...2.1.0.0	-	+IFT122	-	-PLXND1	indel	354 aa	000 nm	
4	>RHO1_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	-MBD4	+IFT122	+H1FOO	-PLXND1	indel	352 aa	000 nm	
5	>RHO1_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2.1.0.0	-MBD4	+IFT122	-	-PLXND1	indel	343 aa	000 nm	
6	>RHO1_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.1.0.0	-MBD4	+IFT122	-	-PLXND1	indel	355 aa	000 nm	
7	>RHO1_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	000 nm	17961206
8	>RHO1_latCha	Latimeria	chalumnae	(coelacanth)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	478 nm	10339578
9	>RHO1_takRub	Takifugu	rubripes	(pufferfish)	Gt	0...2.1.0.0	-MBD4	+IFT122	-	-PLXND1	indel		000 nm	
10	>RHO1_leuEri	Leucoraja	erinacea	(skate)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	000 nm	
11	>RHO1_calMil	Callorhinchus	milii	(elephantfish)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	000 nm	
12	>RHO1_petMar	Petromyzon	marinus	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	354 aa	000 nm	
13	>RHO1_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	354 aa	497 nm	17463225
14	>RHO1_letJap	Lethenteron	japonicum	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	354 aa	000 nm	
15	>RHO2_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	-IHPK3	-LEMD2	-GRM4	+HMGA1	indel	356 aa	000 nm	
16	>RHO2_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2.1.0.0	-IHPK3	-LEMD2	-GRM4	+HMGA1	indel	356 aa	000 nm	
17	>RHO2_gekGek	Gekko	gekko	(gecko)	Gt	0...2.1.0.0	x	x	x	x	indel	356 aa	000 nm	
18	>RHO2_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	356 aa	000 nm	17961206
19	>RHO2_latCha	Latimeria	chalumnae	(coelacanth)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	485 nm	10339578
20	>RHO2_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	492 nm	17463225
21	>SWS2_ornAna	Ornithorhynchus	anatinus	(platypus)	Gt	0...2.1.0.0	-IRAK1	-MECP2	-	+TKTL1	indel	364 aa	000 nm	
22	>SWS2_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	x	x	x	x	indel	362 aa	000 nm	
23	>SWS2_taeGut	Taeniopygia	guttata	(finch)	Gt	0...2.1.0.0	x	x	x	x	indel	363 aa	000 nm	
24	>SWS2_utaSta	Uta	stansburiana	(lizard)	Gt	0...2.1.0.0	x	x	x	x	indel	364 aa	000 nm	
25	>SWS2_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.1.0.0	-IRAK1	-MECP2	-	-	indel	363 aa	000 nm	
26	>SWS2_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	364 aa	000 nm	17961206
27	>SWS2_takRub	Takifugu	rubripes	(pufferfish)	Gt	0...2.1.0.0	x	x	x	x	indel	351 aa	000 nm	genome
28	>SWS2_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gt	0.2.2.1.0.0	x	x	x	x	indel	359 aa	000 nm	genome
29	>SWS2_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	362 aa	439 nm	17463225
30	>SWS1_homSap	Homo	sapiens	(human)	Gt	0.2.2.1.0.0	-FAM137A	-CALU	-NAG6	-FLNC	indel	348 aa	000 nm	
31	>SWS1_monDom	Monodelphis	domesticus	(opossum)	Gt	0...2.1.0.0	-FAM137A	-CALU	-NAG6	-FLNC	indel	347 aa	000 nm	
32	>SWS1_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	x	x	x	x	indel	348 aa	000 nm	
33	>SWS1_taeGut	Taeniopygia	guttata	(finch)	Gt	0...2.1.0.0	x	x	x	x	indel	347 aa	000 nm	
34	>SWS1_anoCar	Anolis	carolinensis	(lizard)	Gt	0.2.2.1.0.0	x	-CALU	x	x	indel	347 aa	000 nm	genome
35	>SWS1_utaSta	Uta	stansburiana	(lizard)	Gt	0...2.1.0.0	x	x	x	x	indel	348 aa	000 nm	
36	>SWS1_xenLae	Xenopus	laevis	(frog)	Gt	0...2.1.0.0	-	-CALU	-	-	indel	348 aa	000 nm	
37	>SWS1_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	347 aa	000 nm	17961206
38	>SWS1_danRer	Danio	rerio	(zebrafish)	Gt	0...2.1.0.0	-	-CALU	-	-	indel	337 aa	000 nm	
39	>SWS1_oryLat	Oryzias	latipes	(medaka)	Gt	0...2.1.0.0	-	-	-	-	indel	336 aa	000 nm	
40	>SWS1_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	346 aa	359 nm	17463225
41	>LWS_homSap	Homo	sapiens	(human)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-TEX28	+TKTL1	indel	364 aa	530 nm	
42	>LWS_monDom	Monodelphis	domesticus	(opossum)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-	+TKTL1	indel	368 aa	000 nm	
43	>LWS_ornAna	Ornithorhynchus	anatinus	(platypus)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-	-	indel	365 aa	000 nm	
44	>LWS_galGal	Gallus	gallus	(chicken)	Gt	0.2.2.1.0.0	x	x	x	x	indel	363 aa	000 nm	
45	>LWS_anoCar	Anolis	carolinensis	(lizard)	Gt	0.2.2.1.0.0	-	-	-TEX28	+TKTL1	indel	370 aa	000 nm	
46	>LWS_xenTro	Xenopus	tropicalis	(frog)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-	-	indel		000 nm	
47	>LWS_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0.2.2.1.0.0	x	x	x	x	indel	365 aa	000 nm	17961206
48	>LWS_takRub	Takifugu	rubripes	(pufferfish)	Gt	0...2.1.0.0	x	x	x	x	indel	358 aa	000 nm	genome
49	>LWS_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gt	0.2.2.1.0.0	-	-	-	-	indel	358 aa	000 nm	genome
50	>LWS_petMar	Petromyzon	maritimus	(lamprey)	Gt	0.2.2.1.0.0	x	x	x	x	indel	366 aa	000 nm	
51	>LWS_letJap	Lethenteron	japonicum	(lamprey)	Gt	0.2.2.1.0.0	x	x	x	x	indel	365 aa	000 nm	
52	>LWS_geoAus	Geotria	australis	(lamprey)	Gt	0.2.2.1.0.0	x	x	x	x	indel	365 aa	560 nm	17463225
53	>PIN_galGal	Gallus	gallus	(chicken)	Gt	0...2.2.0.0					indel	352 aa	000 nm	
54	>PIN_utaSta	Uta	stansburiana	(lizard)	Gt	0...2.2.0.0	x	x	x	x	indel	359 aa	000 nm	
55	>PIN_pheMad	Phelsuma	madagascariensis	(gecko)	Gt	0...2.2.0.0	x	x	x	x	indel	358 aa	000 nm	
56	>PIN_podSic	Podarcis	sicula	(lizard)	Gt	0...2.2.0.0	x	x	x	x	indel	354 aa	000 nm	
57	>PIN_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.2.0.0					indel	346 aa	000 nm	
58	>PIN_bufJap	Bufo	japonicus	(frog)	Gt	0...2.2.0.0	x	x	x	x	indel	347 aa	000 nm	
59	>VAOP_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	+INPP5A	-NXK6	+C10orf61	+ALDH18A1	indel	393 aa	000 nm	
60	>VAOP_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2.1.0.0	+INPP5A	-NXK6	+GPR125	+KNDC1	indel	389 aa	000 nm	
61	>VAOP_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.1.0.0	-	+GSTO2	-C10orf92	-	indel	383 aa	000 nm	
62	>VAOP_danRer	Danio	rerio	(zebrafish)	Gt	0...2.1.0.0	-	-	-	-	indel	378 aa	000 nm	
63	>VAOP_takRub	Takifugu	rubripes	(teleost)	Gt	0...2.1.0.0	+INPP5A	-NXK6	-	+KNDC1	indel	362 aa	000 nm	
64	>VAOP_rutRut	Rutilus	rutilus	(minnow)	Gt	0...2.1.0.0	x	x	x	x	indel	383 aa	000 nm	
65	>VAOP_petMar	Petromyzon	marinus	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	445 aa	000 nm	9427550
66	>PPIN_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2...0.0	-CPEB2	-CACNA2D3	+SELK	+ACTR8	indel	346 aa	000 nm	
67	>PPIN_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2...0.0	-	-	+SELK	-	indel	349 aa	000 nm	
68	>PPIN_ictPun	Ictalurus	punctatus	(catfish)	Gt	0...2...0.0	x	x	x	x	indel	347 aa	000 nm	
69	>PPIN_danRer	Danio	rerio	(zebrafish)	Gt	0...2...0.0	-	-	+SELK	-	indel	338 aa	000 nm	
70	>PPIN_oncMyk	Oncorhynchus	mykiss	(trout)	Gt	0...2...0.0	x	x	x	x	indel	347 aa	000 nm	
71	>PPIN_petMar	Petromyzon	maritimus	(lamprey)	Gt	0...2...0.0	x	x	x	x	indel	344 aa	000 nm	
72	>PPIN_letJap	Lethenteron	japonicum	lamprey	Gt	0...2...0.0	x	x	x	x	indel	344 aa	000 nm	
73	>PPINa_cioInt	Ciona	intestinalis	(tunicate)	Gt	0...2...0.0	-HOXB1	+HHEX	+CUL4A	-	indel	391 aa	000 nm	11591373
74	>PPINb_cioInt	Ciona	intestinalis	(tunicate)	Gt	0...2...0.0	-TMEM165	+FUT4	-	-	indel	353 aa	000 nm	
75	>PARIE_utaSta	Uta	stansburiana	(lizard)	Gd+Go	0...2...0.0	x	x	x	x	indel	347 aa	522 nm	16543463
76	>PARIE_anoCar	Anolis	carolinensis	(lizard)	Gd+Go	0...2...0.0	+EEA1	-FLJ46688	+BTG1	-	indel	347 aa	000 nm	16543463
77	>PARIE_xenTro	Xenopus	tropicalis	(frog)	Gd+Go	0...2...0.0	-lum	-DCN	-	-	indel	346 aa	000 nm	16543463
78	>PARIE_takRub	Takifugu	rubripes	(teleost)	Gd+Go	0...2...0.0	-HSP90B1	+NT5DC2	-KCND3	-FLNC	indel	 351 aa	000 nm	16543463
79	>PARIE_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gd+Go	0...2...0.0	-HSP90B1	+NT5DC2	-KCND3	-FLNC	indel	361 aa	000 nm	-
80	>PARIE_danRer	Danio	rerio	(zebrafish)	Gd+Go	0...2...0.0	-	+NT5DC2	+FBXL13	-	indel	337 aa	000 nm	16543463
81	>ENCEPH_homSap	Homo	sapiens	(human)	Gt	0...2...0.0	-EXO1	-WDR64	-KMO	+FH	indel	403 aa	000 nm	
82	>ENCEPH_monDom	Monodelphis	domestica	(opossum)	Gt	0...2...0.0	-EXO1	-WDR64	-KMO	+FH	indel	411 aa	000 nm	
83	>ENCEPH_galGal	Gallus	gallus	(chicken)	Gt	0...2...0.0	-EXO1	-WDR64	-PIGM	+RGS7	indel	396 aa	000 nm	
84	>ENCEPH_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2...0.0	-EXO1	-WDR64	-PIGM	+RGS7	indel	408 aa	000 nm	
85	>ENCEPH_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2...0.0	-ASAH3L	+RPS6	-ADFP	-DENND4C	indel	401 aa	000 nm	
86	>ENCEPH_takRub	Takifugu	rubripes	(teleost)	Gt	0...2...0.0	-ABLIM1	+PTK7	-KMO	+IDE	indel	388 aa	000 nm	
87	>ENCEPH4a_takRub	Takifugu	rubripes	(teleost)	Gt	0...2...0.0	-CALD1	+TNK2	-RAB18	+ABI1	indel	403 aa	000 nm	
88	>ENCEPH4b_takRub	Takifugu	rubripes	(teleost)	Gt	0...2...0.0	+TFRC	+CHES1	-MYEOV2	-ARHGAP21	indel	407 aa	000 nm	
89	>ENCEPH_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gt	0...2...0.0	-LDOC1L	+CDC42EP3	-KMO	+IDE	indel	389 aa	000 nm	
90	>ENCEPH4_braFlo	Branchiostoma	floridae	(amphioxus)	Gt		-ZFYVE1	+RTF1	-CES1	-POMT2	indel	402 aa	000 nm	
91	>ENCEPH4_braBel	Branchiostoma	belcheri	(amphioxus)	Gt		x	x	x	x	indel	401 aa	000 nm	
92	>ENCEPH5_braFlo	Branchiostoma	floridae	(amphioxus)	Gt		-ZFYVE1	+RTF1	+ATP6V0E1	-Etf1	indel	409 aa	000 nm	
93	>ENCEPH5_braBel	Branchiostoma	belcheri	(amphioxus)	Gt		x	x	x	x	indel	421 aa	000 nm	
94	>ENCEPH_apiMel	Apis	mellifera	(bee)	Gt						indel	329 aa	000 nm	
95	>ENCEPH1_anoGam	Anopheles	gambiae	(mosquito)	Gt						indel	461 aa	000 nm	
96	>ENCEPH2_anoGam	Anopheles	gambiae	(mosquito)	Gt						indel	434 aa	000 nm	
97	>CILL2_plaDum	Platynereis	dumerilii	(ragworm)	Gt		x	x	x	x	indel	310 aa	000 nm	
98	>CILL1_plaDum	Platynereis	dumerilii	(ragworm)	Gt		x	x	x	x	indel	355 aa	000 nm	
99	>RGR_homSap	Homo	sapiens	(human)	??		+PCDH21	-LRIT1	-GRID1	-WAPAL	indel	296 aa	000 nm	
100	>RGR_galGal	Gallus	gallus	(chicken)	??		+PCDH21	-LRIT1	+CHAT	-PARG	indel	296 aa	000 nm	14985289
101	>RGR_xenTro	Xenopus	tropicalis	(frog)	??		+PCDH21	-LRIT1	+CHAT	-PARG	indel	296 aa	000 nm	-
102	>RGR_gasAcu	Gasterosteus	aculeatus	(stickleback)	??		+PCDH21	-LRIT1	+CHAT	-PARG	indel	296 aa	000 nm	-
103	>PER_homSap	Homo	sapiens	(human)	??		-CFI	+NOLA1	+EGF	-ELOVL6	indel	338 aa	000 nm	
104	>PER_monDom	Monodelphis	domestica	(opossum)	??		-CFI	+NOLA1	+EGF	-ELOVL6	indel	326 aa	000 nm	-
105	>PER_galGal	Gallus	gallus	(chicken)	??		-CFI	+NOLA1	+EGF	-ELOVL6	indel	335 aa	000 nm	14985289
106	>PER_xenTro	Xenopus	tropicalis	(frog)	??		-CFI	+NOLA1	+EGF	-ELOVL6	indel	347 aa	000 nm	
107	>PER_gasAcu	Gasterosteus	aculeatus	(stickleback)	??		+GPR68	-GNPDA1	-ENPEP	-C14orf100				
108	>PERa_braBel	Branchiostoma	belcheri	(amphioxus)	??		x	x	x	x	indel	365 aa	000 nm	
109	>PERb_braBel	Branchiostoma	belcheri	(amphioxus)	??		x	x	x	x	indel	522 aa	000 nm	
110	>PERc_braBel	Branchiostoma	belcheri	(amphioxus)	Go		x	x	x	x	indel	391 aa	000 nm	
111	>NEUR_homSap	Homo	sapiens	(human)	??		+CD2AP	+GPR115	-C6orf138	-MUT	indel	355 aa	000 nm	
112	>MEL1_homSap	Homo	sapiens	(human)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel	479 aa	000 nm	
113	>MEL1_monDom	Monodelphis	domestica	(opossum)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel		000 nm	
114	>MEL1_galGal	Gallus	gallus	(chicken)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel		000 nm	16856781
115	>MEL1_xenTro	Xenopus	tropicalis	(frog)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel	596 aa	000 nm	16856781
116	>MEL1_danRer	Danio	rerio	(zebrafish)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm	
117	>MEL1D_danRer	Danio	rerio	(zebrafish)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm	
118	>MEL1_takRub	Takifugu	rubripes	(teleost)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm	
119	>MEL1_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gq		-	-	+LDB3	+BMPR1A	indel		000 nm	
120	>MEL1_oryLat	Oryzias	latipes	(medaka)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm	
121	>MEL1_calMil	Callorhinchus	milii	(elephantfish)	Gq		-	-	-	-	indel		000 nm	
122	>MEL1a_braFlo	Branchiostoma	floridae	(amphioxus)	Gq		-	-	-	-	indel	709 aa	000 nm	
123	>MEL1a_braBel	Branchiostoma	belcheri	(amphioxus)	Gq		x	x	x	x	indel	707 aa	000 nm	
124	>MEL1b_braFlo	Branchiostoma	floridae	(amphioxus)	Gq		-	-	-	-	indel		000 nm	
125	>MEL1b_braBel	Branchiostoma	belcheri	(amphioxus)	Gq		x	x	x	x	indel	402 aa	000 nm	
126	>MEL2_galGal	Gallus	gallus	(chicken)	Gq		+GRID2+SMARCAD1	-PGDS	-SEC24B	+COL25A1	indel	544 aa	000 nm	17977531
127	>MEL2_anoCar	Anolis	carolinensis	(lizard)	Gq		+GRID2+SMARCAD1	-ATOH1	+PDLIM5	+BMPR1B	indel	290 aa	000 nm	
128	>MEL2_xenLae	Xenopus	laevis	(frog)	Gq		+SMARCAD1		+PDLIM5	+BMPR1B	indel	535 aa	000 nm	
129	>MEL2_danRer	Danio	rerio	(zebrafish)	Gq		-	+FLJ39155	+PDLIM5	-	indel		000 nm	
130	>MEL2_tetNig	Tetraodon	nigroviridis	(pufferfish)	Gq		-	-	-	+BMPR1B	indel	404 aa	000 nm	
131	>MEL2_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gq		KNTC2	+FLJ39155	+PDLIM5	+BMPR1B	indel	353 aa	000 nm	
132	>RHAB_plaDum	Platynereis	dumerilii	(polychaete)	Gq		x	x	x	x	indel	383 aa	000 nm	
133	>RHAB1_apiMel	Apis	mellifera	(bee)	Gq						indel	378 aa	000 nm	
134	>RHAB2_apiMel	Apis	mellifera	(bee)	Gq						indel	386 aa	000 nm	
135	>RHAB3_apiMel	Apis	mellifera	(bee)	Gq						indel	372 aa	000 nm	
136	>RHAB4_apiMel	Apis	mellifera	(bee)	Gq						indel	378 aa	000 nm	
														
														
1	>RHO1_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	complete	000 nm	
2	>LWS_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
3	>PIN_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
4	>PPIN_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
5	>VAOP_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
6	>RGR_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
7	>PER_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
8	>ENCEPH_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
9	>ENCEPH4_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
10	>ENCEPH5_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
11	>MEL1b_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm	
12	>ENCEPH_squAca	Squalus	acanthias	(dogfish)			x	x	x	x	indel	fragment	000 nm	
13	>ENCEPH_petMar	Petromyzon	marinus	(lamprey)			x	x	x	x	indel	fragment	000 nm	
14	>MEL1_petMar	Petromyzon	marinus	(lamprey)			x	x	x	x	indel	fragment	000 nm	
15	>PARIE_petMar	Petromyzon	marinus	(lamprey)			x	x	x	x	indel	fragment	000 nm

>RHO1_homSap rhodopsin ciliary all GT-AG chr3:130,730,172 3q22.1 + 6706 5 12 21 00 00
0 MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG 1
2 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSR 2
1 YIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQ 0
0 FRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA* 0

>RHO1_monDom Monodelphis opossum 349
0 MNGTEGPNFYVPFSNKTGTVRSPFEEPQYYLADPWQFSCLAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAIADLFMVFGGFTMTLYTSLHGYFVFGPTGCNLEGFFATLG 1
2 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIIGVAFTWVMALACAFPPLIGWSR 2
1 YIPEGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPLIVIFFCYGQLVFTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSNFGPIFMTIPAFFAKSSSVYNPVIYIMMNKQ 0
0 FRTCMITTLCCGKNPLGDDEASATASKTETSQVAPA* 0

>RHO1_ornAna rhodopsin rod pigment Ultra20:114,479-118,391
0 MNGTEGQDFYIPMSNKTGVVRSPFEYPQYYLAEPWQYSVLAAYMFMLIMLGFPINFLTLYVTIQHKKLRTPLNYILLNLAFANHFMVLGGFTTTLYTSLHGYFVFGPTGCNIEGFFATLG 1
2 GEIALWSLVVLAIERYIVVCKPMSNFRFGENHAIMGVAFTWIMALACALPPLVGWSR 2
1 YIPEGMQCSCGIDYYTLRPEVNNESFVIYMFVVHFTIPMTIIFFCYGRLVFTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTVPAFFAKSSAIYNPVIYIMMNKQ 0
0 FRNCMLTTICCGKNPLGDDEASATASKTEQSSVSTSQVSPA* 0

>RHO1_galGal RH1 RDP1 rhodopsin rod galGal+ ornAna+ homSap+
0 MNGTEGQDFYVPMSNKTGVVRSPFEYPQYYLAEPWKFSALAAYMFMLILLGFPVNFLTLYVTIQHKKLRTPLNYILLNLVVADLFMVFGGFTTTMYTSMNGYFVFGVTGCYIEGFFATLG 1
2 GEIALWSLVVLAVERYVVVCKPMSNFRFGENHAIMGVAFSWIMAMACAAPPLFGWSR 2
1 YIPEGMQCSCGIDYYTLKPEINNESFVIYMFVVHFMIPLAVIFFCYGNLVCTVKE 0
0 AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTNQGSDFGPIFMTIPAFFAKSSAIYNPVIYIVMNKQ 0
0 FRNCMITTLCCGKNPLGDEDTSAGKTETSSVSTSQVSPA* 0

>RHO1_anoCar Anolis lizard
0 MNGTEGQNFYVPMSNKTGVVRNPFEYPQYYLADPWQFSALAAYMFLLILLGFPINFLTLFVTIQHKKLRTPLNYILLNLAVANLFMVLMGFTTTMYTSMNGYFIFGTVGR 2
2 GEMGLWSLVVLAVERYVVICKPMSNFRFGETHALIGVSCTWIMALACAGPPLLGWSR 2
1 YIPEGMQCSCGVDYYTPTPEVHNESFVIYMFLVHFVTPLTIIFFCYGRLVCTVKA 0
0 AAAQQQESATTQKAEREVTRMVVIMVISFLVCWVPYASVAFYIFTHQGSDFGPVFMTIPAFFAKSSAIYNPVIYILMNKQ 0
0 FRNCMIMTLCCGKNPLGDEDTSAGTKTETSTVSTSQVSPA* 0

>RHO1_xenTro frog 354
0 MNGTEGPNFYIPMSNKTGVVRSPFDYPQYYLAEPWKYSALAAYMFLLILLGFPINFMTLYVTIQHKKLRTPLNYILLNLVFANHFMVLCGFTVTMYTSMHGYFIFGQTGCYIEGFFATLG 1
2 GEMALWSLVVLAIERYVVVCKPMANFRFGENHAIMGVVFTWIMALSCAAPPLFGWSR 2
1 YIPEGMQCSCGVDYYTLKPEVNNESFVVYMFIVHFTIPLCVIFFCYGRLLCTVKE 0
0 AAAQQQESATTQKAEKEVTRMVVMMVIFFLICWVPYAYVAFYIFTHQGSDFGPVFMTVPAFFAKSSAIYNPVIYIVLNKQ 0
0 FRNCLITTLCCGKNPFGDEEGSSAASSKTEASSVSSSQVSPA* 0

>RHO1_neoFor Neoceratodus forsteri (Australian lungfish)
0 MNGTEGPNFYVPMTNKTGVVRSPFEYPQYYLADPWKYSALAAYMFFLILTGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTMYTAMNGYFVFGVVGCNLEGFFATFG 1
2 GIIALWCLVVLAIERYIVVCKPISNFRFGENHAIMGVVFTWIMALACAGPPLFGWSR 2
1 YIPEGMQCSCGIDYYTLKPEVNNESFVIYMFIVHFTIPLIIIFFCYGRLMCTVKE 0
0 AAAQQQESATTQKAEKEVTRMVYIMVISYLVCWLPYASVSFYIFTHQGSDFGPVFMTVPAFFAKTASVYNPVIYILMNKQ 0
0 FRNCMITTLCCGKNPFGDEETTSAGTSKTEASSVSSSQVSPA* 0

>RHO1_latCha Latimeria chalumnae (coelacanth)
0 MNGTEGPNFYVPMSNKTGVVRNPFEYPQYYLADPWKYSALAAYMFFLILVGFPINFLTLFVTIQHKKLRTPLNYILLDLAVADLCMVFGGFFVTMYSSMNGYFVLGPTGCNIEGFFATLG 1
2 GQVALWALVVLAIERYVVVCKPMSNFRFGENHAIMGVIFTWIMALSCAVPPLFGWSR 2
1 YIPEGMQSSCGVDYYTLKPEVNNESFVIYMFVVHFTIPLIVIFFCYGRLVCTVKD 0
0 AAAQQQESATTQKAEKEVTRMVIVMVISFLVCWVPYASVAAYIFFNQGSEFGPVFMTAPSFFAKSASFYNPVIYILLNKQ 0
0 FRNCMITTLCCGKNPFGDEDATSAAGSSKTEASSVSSSSVSPA* 0

>RHO1_takRub Takifugu rubripes (pufferfish)
0 MNGTEGPNFYIPMSNKTGVVRSPFEYPQYYLAEPWKYSLVAAYMLFLIITAFPVNFLTLFVTVKHKKLRTPLNYVLLNLAVADLFMVIGGFTVTLYTALHAYFVLGVTGCNIEGFFATLG 1
2 GEIALWSLVVLAVERYIVVCKPMTNFRFGEKHAIAGLVFTWIMALTCATPPLLGWSR 2
1 YIPEGMQCSCGIDYYTPKPEINNTSFVIYMFILHFSIPLAIIFFCYSRLLCTVRA 0
0 AAALQQESETTQRAEKEVTRMVIVMVISFLVCWVPYASVAWYIFANQGTEFGPVFMTAPAFFAKSAALYNPVIYILLNRQ 0
0 FRNCMITTVCCGKNPFGDDDAATTVSKTQSSSVSSSQVAPA* 0

>RHO1_leuEri Leucoraja erinacea rod opsin 
0 MNGTEGENFYVPMSNKTGVVRSPFDYPQYYLGEPWMFSALAAYMFFLILTGLPVNFLTLFVTIQHKKLRQPLNYILLNLAVSDLFMVFGGFTTTIITSMNGYFIFGPAGCNFEGFFATLG 1
2 GEVGLWCLVVLAIERYMVVCKPMANFRFGSQHAIIGVVFTWIMALSCAGPPLVGWSR 2
1 YIPEGLQCSCGVDYYTMKPEVNNESFVIYMFVVHFTIPLIVIFFCYGRLVCTVKE 0
0 AAAQQQESESTQRAEREVTRMVIIMVVAFLICWVPYASVAFYIFINQGCDFTPFFMTVPAFFAKSSAVYNPLIYILMNKQ 0
0 FRNCMITTICLGKNPFEEEESTSASASKTEASSVSSSQVAPA* 0

>RHO1_calMil Callorhinchus milii complete wgs
0 MNGTEGENFYIPMSNKTGVVRSPFEYPQYYLAEPWQFSILAAYMFFLIITCFPVNFLTLYVTFEHKKLRQPLNFILLNLAVADLFMVFGGFFITVYTSLHGYFVFGVTGCNFEGFFATLG 1
2 GEIGLWSLVVLAIERYVVVCKPMSNFRFGTNHAIMGVAFTWVMALACAVPPLMGWSR 2
1 YIPEGLQCSCGVDYYTLKPEINNESFVIYMFVVHFLIPLIIIFFCYGRLVCTVKE 0
0 AAAQQQESESTQRAEREVTRMVIIMVIFFLICWVPYASVAFFIFTNQGSEFGPIFMAVPAFFAKSSALYNPLIYILLNKQ 0
0 FRNCMITTLCCGKNPFEEDESTSAAASKTEASSVSSSQVSPA* 0

>RHO1_petMar lamprey Petromyzon marinus introns from U67123
0 MNGTEGENFYIPFSNKTGLARSPFEYPQYYLAEPWKYSVLAAYMFFLILVGFPVNFLTLFVTVQHKKLRTPLNYILLNLAVANLFMVLFGFTLTMYSSMNGYFVFGPTMCNFEGFFATLG 1
2 GEMSLWSLVVLAIERYIVICKPMGNFRFGSTHAYMGVAFTWFMALSCAAPPLVGWSR 2
1 YLPEGMQCSCGPDYYTLNPNFNNESFVIYMFLVHFIIPFIVIFFCYGRLLCTVKE 0
0 AAAAQQESASTQKAEKEVTRMVVLMVIGFLVCWVPYASVAFYIFTHQGSDFGATFMTVPAFFAKTSALYNPIIYILMNKQ 0
0 FRNCMITTLCCGKNPLGDEDSGASTSKTEVSSVSTSQVSPA* 0

>RHO1_geoAus lamprey Geotria australis rodRhA introns by homology
0 MNGTEGQNFYIPFSNKTDVARSPFEYPQYYLAEPWKFSALAAYMFFLILVGFPVNFLTLFVTVQHKKLRTPLNYILLNLAVSNLFMILFGFTTTMYTSMNGYFVFGPTMCSIEGFFATLG 1
2 GEVSLWSLVVLAIERYIVICKPMGNFRFGNTHAIMGVALTWVMALSCAAPPLLGWSR 2
1 YLPEGMQCSCGPDYYTMNPTYNNESFVIYMFIVHFTIPFVIIFFSYGRLLCTVKE 0
0 AAAAQQESASTQKAEKEVTRMVVLMVVGFLVCWVPYASVAFYIFTNQGSDFGATFMTLPAFFAKSSALYNPVIYILMNKQ 0
0 FRNCMITTLCCGKNPLGDDDSGASTSKTEVSSVSTSQVAPA* 0

>RHO1_letJap lamprey Lethenteron japonicum introns by homology
0 MNGTEGDNFYVPFSNKTGLARSPYEYPQYYLAEPWKYSALAAYMFFLILVGFPVNFLTLFVTVQHKKLRTPLNYILLNLAMANLFMVLFGFTVTMYTSMNGYFVFGPTMCSIEGFFATLG 1
2 GEVALWSLVVLAIERYIVICKPMGNFRFGNTHAIMGVAFTWIMALACAAPPLVGWSR 2
1 YIPEGMQCSCGPDYYTLNPNFNNESYVVYMFVVHFLVPFVIIFFCYGRLLCTVKE 0
0 AAAAQQESASTQKAEKEVTRMVVLMVIGFLVCWVPYASVAFYIFTHQGSDFGATFMTLPAFFAKSSALYNPVIYILMNKQ 0
0 FRNCMITTLCCGKNPLGDDESGASTSKTEVSSVSTSQVSPA* 0

>RHO2_galGal rhodopsin-like cone galGal+ ornAna- homSap-
0 MNGTEGINFYVPMSNKTGVVRSPFEYPQYYLAEPWKYRLVCCYIFFLISTGLPINLLTLLVTFKHKKLRQPLNYILVNLAVADLFMACFGFTVTFYTAWNGYFVFGPVGCAVEGFFATLG 1
2 GQVALWSLVVLAIERYIVVCKPMGNFRFSATHAMMGIAFTWVMAFSCAAPPLFGWSR 2
1 YMPEGMQCSCGPDYYTHNPDYHNESYVLYMFVIHFIIPVVVIFFSYGRLICKVRE 0
0 AAAQQQESATTQKAEKEVTRMVILMVLGFMLAWTPYAVVAFWIFTNKGADFTATLMAVPAFFSKSSSLYNPIIYVLMNKQ 0
0 FRNCMITTICCGKNPFGDEDVSSTVSQSKTEVSSVSSSQVSPA* 0

>RHO2_anoCar rhodopsin-like cone anoCar+ HMGA1 GRM4 RHO2 MLN LEMD2 IHPK3
0 MNGTEGINFYVPLSNKTGLVRSPFEYPQYYLAEPWKYKVVCCYIFFLIFTGLPINILTLLVTFKHKKLRQPLNYILVNLAVADLFMACFGFTVTFYTAWNGYFIFGPIGCAIEGFFATLG 1
2 GQVALWSLVVLAIERYIVVCKPMGNFRFSATHALMGISFTWFMSFSCAAPPLLGWSR 2
1 YIPEGMQCSCGPDYYTLNPDYHNESYVLYMFGVHFVIPVVVIFFSYGRLICKVRE 0
0 AAAQQQESASTQKAEREVTRMVILMVLGFLLAWTPYAMVAFWIFTNKGVDFSATLMSVPAFFSKSSSLYNPIIYVLMNKQ 0
0 FRNCMITTICCGKNPFGDEDVSSSVSQSKTEVSSVSSSQVSPA* 0

>RHO2_gekGek Gekko gekko 467 Cone visual pigments are present in gecko rod cells
0 MNGTEGINFYVPLSNKTGLVRSPFEYPQYYLADPWKFKVLSFYMFFLIAAGMPLNGLTLFVTFQHKKLRQPLNYILVNLAAANLVTVCCGFTVTFYASWYAYFVFGPIGCAIEGFFATIG 1
2 GQVALWSLVVLAIERYIVICKPMGNFRFSATHAIMGIAFTWFMALACAGPPLFGWSR 2
1 FIPEGMQCSCGPDYYTLNPDFHNESYVIYMFIVHFTVPMVVIFFSYGRLVCKVRE 0
0 AAAQQQESATTQKAEKEVTRMVILMVLGFLLAWTPYAATAIWIFTNRGAAFSVTFMTIPAFFSKSSSIYNPIIYVLLNKQ 0
0 FRNCMVTTICCGKNPFGDEDVSSSVSQSKTEVSSVSSSQVAPA* 0

>RHO2_neoFor Neoceratodus forsteri (Australian lungfish)
0 MNGTEGINFYVPHSNKTGVVRSPFEYPQYYLADPWKYSIVCAYMFFLIITGLPINLLTLVVTFKHKKLRQPLNYILVNLAVADLFMVCFGFTVTFSTAINGYFIFGPRGCAIEGFMATLG 1
2 GEVALWSLVVLAIERYIVVCKPMGNFRFSNNHSIIGIVFTWLAALSCAAPPLFGWSR 2
1 YLPEGMQCSCGPDYYTMNPDYHNESFVIYMFVVHFFIPVIVIFVSYGRLICKVKE 0
0 AAAQQQESASTQKAEREVTRMVILMVIGFMTAWTPYATVAFWIFMNKGAEFGATFMAAPAFFSKSSALYNPIIYVLMNKQ 0
0 FRNCMVTTLCCGKNPFGDDDVSSSVSAGKTEVSSVSSSQVSPA* 0

>RHO2_latCha Latimeria chalumnae (coelacanth) RH2 opsin
0 MNGTEGMNFYVPLSNRTGLVRSPFEYTQYYLAEPWKFSVLCAYMFLLIILGFPINFLTLLVTFKHKKLRQPLNYILVNLAVASLFMVVFGFTVTFYSSLNGYFVLGPMGCAMEGFFATLG 1
2 GQVALWSLVVLAIERYIVVCKPMGNFRFASSHAIMGIAFTWIMALACAAPPLVGWSR 2
1 YIPEGLQCSCGPDYYTLNPDFHNESYVMYLFLVHFLLPIIIIFFTYGRLICKVKE 0
0 AAAQQQESASTQKAEKEVTRMVILMVIGFLTAWVPYASAAFWIFCNRGAEFTATLMTVPAFFSKSSCLFNPIIYVLLNKQ 0
0 FRNCMITTLCCGKNPLGDDDTSSAVSQSKTDVSSVSSSQVSPA* 0

>RHO2_geoAus Geotria australis lamprey rod RhB lamprey
0 MNGTEGANFYIPFHNRTGVVRSPYEYPQYYLADPWMYSAISAYVFTLILIGFPVNFMTLFVTFKLKKLRQPLNFILVNLCVADLLMIMFGFTTTFYTAMNGYFVFGPTGCNIEGFFATLG 1
2 GEVSLWSLVMLAIERYIVVCKPMGNFRFATTHAALGVVFTWVMASACAVPPLVGWSR 2
1 YIPEGMQCSCGPDYYTLNPKYYNESYVIYLFLVHFLLPVTIIFFTYGRLICTVKE 0
0 AAAQQQESASTQKAEREVTRMVIIMVVGFLVCWVPYASFAFYLFMNKGILFSATAMTVPAFFSKSSVLYNPIIYVLLNKQ 0
0 FRTCMVTTLFCGKNPFGEDDSSMVSTSKTEVSSVSSSQVSPS* 0

>SWS2_ornAna platypus blue cone bp 2 gene tandem -FLNB +MECP2 with MWS1 5 usual exons
0 MHKTHRNLQNELPEDFFIPLPLDTDNITSLSPFLVPQTHLGGSGIFMSLAAFMFLLITLGFPINLLTVICTIKYKKLRSHLNYILVNLAVSNMLVVCVGSATAFYSFAHMYFVLGPTACKIEGFAATLG 1
2 GMVSLWSLAVIAFERFLVICKPLGNLSFRGTHAIFGCAATWVFGLAASLPPLFGWSR 2
1 YIPEGLQCSCGPDWYTTNNKWNNESYVIFLFSFCFGVPLSIIIFSYGRLLLTLRA 0
0 VAKQQEQSATTQKAEREVTKMVIVMVLGFLVCWLPYASFSLWVVTNRGQVFDLRMASIPSVFSKASTIYNPIIYVFMNKQ 0
0 FRSCMLKLVFCGKSPFGDEDEISGSSQATQVSSVSSSQVSPA* 0

>SWS2_galGal chicken blue cone pigment galGal- ornAna+ homSap+ 
0 MHPPRPTTDLPEDFYIPMALDAPNITALSPFLVPQTHLGSPGLFRAMAAFMFLLIALGVPINTLTIFCTARFRKLRSHLNYILVNLALANLLVILVGSTTACYSFSQMYFALGPTACKIEGFAATLG 1
2 GMVSLWSLAVVAFERFLVICKPLGNFTFRGSHAVLGCVATWVLGFVASAPPLFGWSR 2
1 YIPEGLQCSCGPDWYTTDNKWHNESYVLFLFTFCFGVPLAIIVFSYGRLLITLRA 0
0 VARQQEQSATTQKADREVTKMVVVMVLGFLVCWAPYTAFALWVVTHRGRSFEVGLASIPSVFSKSSTVYNPVIYVLMNKQ 0
0 FRSCMLKLLFCGRSPFGDDEDVSGSSQATQVSSVSSSHVAPA* 0

>SWS2_taeGut Taeniopygia guttata
0 MPKPREMRDELPEDFYIPMSLETPNLTALSPFLVPQTHLGSPGIFKAMAAFMFLLVLLGVPINALTVLCTAKYKKLRSHLNYILVNLAVANLLVVCVGSTTAFYSFSQMYFALGPLACKIEGFTATLG 1
2 GMVSLWSLAVVAFERFLVICKPLGNFTFRGSHAVLGCAITWIFGLIASLPPLFGWSR 2
1 YIPEGLQCSCGPDWYTTDNKWNNESYVIFLFCFCFGFPLTVIVFSYGRLLLTLRA 0
0 VAKQQEQSASTQKAEREVTKMVVVMVLGFLVCWLPYCSFALWVVTHRGHPFDLGLASIPSVFSKASTVYNPIIYVFMNKQ 0
0 FRSCMLKLVFCGRSPFGDEDDVSGSSQATQVSSVSSSQVSPA* 0 

>SWS2_utaSta lizard
0 MHNSRPHSRDDLPEDFFIPMPLDVANITTLSPFLVPQTHLGSPALFMGMAAFMFLLIILGVPINVLTIFCTFKYKKLRSHLNYILVNLAVSNLLVVCIGSTTAFYSFAQMYFSLGPTACKIEGFAATLG 1
2 GMVSLWSLAVVAFERFLVICKPLGNFSFRGTHAIIGCIITWVFGLVASLPPLFGWSR 2
1 YIPEGLQCSCGPDWYTTNNKWNNESYVLFLFSFCFGVPLSVIIFSYGRLLLTLRA 0
0 VAKQQEQSATTQKAEREVTKMVVVMVMGFLVCWLPYASFALWVVTHRGEPFDVRLATIPSVFSKASSVYNPVIYVFMNKQ 0
0 FRSCMLKLVFCGKSPFGDEDDVSGSSQTTQVSSVSSSQVSPA* 0

>SWS2_xenTro Xenopus tropicalis
0 MSKGRPDLRMEMPDEFYVPIPLETTNISSLSPFLVPQTHLGTPGIFMSISAFMLFTIIFGFPLNLLTIICTVKYKKLRSHLNYILVNLAVANLIVICFGSTTAFYSFSQMYFSLGTLACKIEGFTATLG 1
2 GIIGLWSLAVVAFERFLVICKPMGNFTFRESHAVLGCILTWVIGLVAAIPPLLGWSR 2
1 YIPEGLQCSCGPDWYTVNNKWNNESYVLFLFCFCFGFPLAIIVFSYGRLLLALHA 0
0 VAKQQEQSATTQKAEREVTRMVIVMVVGFLVCWLPYASFALWAVTHRGELFDLRMSSVPSVFSKASTVYNPFIYIFMNRQ 0
0 FRSCMMKMIFCGKNPLGDDEETSVSGSTQVSSVSSSQIAPS* 0 

>SWS2_neoFor Neoceratodus forsteri (Australian lungfish)
0 MHRTKPDPQEDLPDDFYIPVSLNTNNITMLSPFLVPQTHLGSPSVFMVLSVFMFFLLITGIPINVLTIICTFKYKKLRSHLNYILVNLAVANLIVVGFGSTTAFYSFSQMYFAWGPLACKIEGFAATLG 1
2 GMVSLWSLAVVAFERFLVICKPLGNFTFRSTHAIIGCVATWVFGLISSAPPLFGWSR 2
1 YIPEGLQCSCGPDWYTTNNKWNNESYVIFLFCFCFGFPLSVIIFSYGRLLMTLRA 0
0 VAKQQEQSASTQKAEREVTKMVVVMVLGFLVCWLPYTVFSLWVVTHRGESFELALGSIPAVFSKSSTVYNPLIYVFMNKQ 0
0 FRSCMMKLIFCGKSPFGDEDDASSASQSTQVSSVSSSQVAPA* 0

>SWS2_takRub Takifugu rubripes
0 MRGVRQHEFQEDFYIPIPLDVDNITALSPFLVPQDHLGSPAVFYGMSAFMFFLFVAGTGINVLTIACTIQYKKLRSHLNYILVNLAFSNLLVTTVGSFTCFCCFFVRYMIVGPLGCKIEGFAATLG 1
2 GMVSLWSLAVVAFERWLVVCKPLGNFIFKPDHAIVCCIFTWFFALIISAPPLFGWSR 2
1 YIPEGFQCSCGPDWYTTGNKYNNESYVWFIFGFGFAVPLFVIVFCYSQLLVMLKS 0
0 AKAQAESASTQKAEREVTRMVVVMILGFLVCWLPYASFALWVVNNRGTPFDLRLATIPACFSKASTVYNPIIYVVLNKQ 0
0 FRSCMKKMLGMSGGDDEESSSQSVTEVSKVSPS* 0 

>SWS2_gasAcu Gasterosteus aculeatus (stickleback) 359 aa
0 MKHGRVPEIPEDFYIPISLDTDNITSLSPFLVPQDHLASKATFYSLAFYMFFILIVGTFINALTVACTVQNKKLRSHLNYILVNLAVSNLLVSGVGAFTAFLSFAARYFVLGTLACKVEGFLATLG 1
2 GMVSLWSLAVIAFERWLVICKPLGNFIFKPDHALVCCAFTWVFALAASAPPLVGWSR 2
1 YIPEGLQCSCGPDWYTTNNKYNNESYVLFLFGFCFAVPFCTICFCYSQLLFTMKMA 0
0 AKAQAESASTQKAEREVTRMVVLMVMGFLVCWMPYASFALWVVNNRGQTFDLRFASIPSVFSKSSAVYNPVIYVLLNKQ 0
0 FRSCMMKMLGMGGGDDEESSTSSVTEVSKVGPA* 0

>SWS2_geoAus Geotria australis lamprey blue-sensitive retinal pigment lamprey
0 MYQGKSTQVDDLPEDFYIPIALNVKNMSELSPFLVPQVHLGDSFIFYGMSAFMLFLVLAGFPLNFLTVFVTIKYKKLRSHLNYILVNLAIANLIVVCCGSTLAFYSFMHKYFILGPLFCKMEGFTATLG 1
2 GMLSLWSLAVLAFERCLVICKPFGNIAFRGTHALIRCGFAWAAAIAASTPPLFGWSR 2
1 YIPEGLQCSCGPDWYTTNNKYNNESYVMFLFIFCFGTPFTIIIVSYSKLILTLRA 0
0 AAAQQQESASTQKAEKEVSRMVVIMVGGFLVCWLPYASLALWIVFNRGSPFDLRLATIPSVFSKASTVYNPVIYIFLNKQ 0
0 FRSCMMKTIFCGKNPLGDDEDATSTTTQVSSVSTSQVAPA* 0

>SWS1_homSap 5 exons all GT-AG Blue opsin chr7:128,199,783-128,203,087 7q32.1 - 3305 5 348 (tritanopia) [MIM:190900].
0 MRKMSEEEFYLFKNISSVGPWDGPQYHIAPVWAFYLQAAFMGTVFLIGFPLNAMVLVATLRYKKLRQPLNYILVNVSFGGFLLCIFSVFPVFVASCNGYFVFGRHVCALEGFLGTVA 1
2 GLVTGWSLAFLAFERYIVICKPFGNFRFSSKHALTVVLATWTIGIGVSIPPFFGWSR 2
1 FIPEGLQCSCGPDWYTVGTKYRSESYTWFLFIFCFIVPLSLICFSYTQLLRALKA 0
0 VAAQQQESATTQKAEREVSRMVVVMVGSFCVCYVPYAAFAMYMVNNRNHGLDLRLVTIPSFFSKSACIYNPIIYCFMNKQ 0
0 FQACIMKMVCGKAMTDESDTCSSQKTEVSTVSSTQVGPN* 0

>SWS1_monDom Monodelphis domesticus
0 MSGDEEFYLFKNISSVGPWDGPQYHIAPAWAFHFQTVFMGFVFCAGTPLNAVVLVATLRYKKLRQPLNYILVNVSLCGFIFCIFAVFTVFISSSQGYFIFGRHVCAMEAFLGSVA 1
2 GLVTGWSLAFLAFERFIVICKPFGNFRFNSKHAMMVVLATWVIGIGVSIPPFFGWSR 2
1 FIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIMPLFLICFSYSQLLRALRA 0
0 VAAQQQESATTQKAEREVSRMVVMMVGSFCLCYVPYAALAMYMVNNQNHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ 0
0 FHACIMEMVCRKPMTDDSDVSSSQKTEVSAVSSSQVGPT* 0

>SWS1_galGal chicken violet cone pigment
0 MSSDDDFYLFTNGSVPGPWDGPQYHIAPPWAFYLQTAFMGIVFAVGTPLNAVVLWVTVRYKRLRQPLNYILVNISASGFVSCVLSVFVVFVASARGYFVFGKRVCELEAFVGTHG 1
2 GLVTGWSLAFLAFERYIVICKPFGNFRFSSRHALLVVVATWLIGVGVGLPPFFGWSR 2
1 YMPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLIIFSYSQLLSALRA 0
0 VAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRDHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ 0
0 FRACIMETVCGKPLTDDSDASTSAQRTEVSSVSSSQVGPT* 0

>SWS1_taeGut Taeniopygia guttata
0 MDEEEFYLFKNQSSVGPWDGPQYHIAPMWAFYLQTIFMGLVFVAGTPLNAIVLIVTIKYKKLRQPLNYILVNISVSGLMCCVFCIFTVFIASSQGYFVFGKHMCAFEGFAGATG 1
2 GLVTGWSLAFLAFERYIVICKPFGNFRFNSRHALLVVAATWIIGVGVAIPPFFGWSR 2
1 YIPEGLQCSCGPDWYTVGTKYKSEYYTWFLFIFCFIVPLSLIIFSYSQLLSALRA 0
0 VAAQQQESATTQKAEREVSRMVVVMVGSFCMCYVPYAALAMYMVNNREHGIDLRLVTIPAFFSKSSCVYNPIIYCFMNKQ 0
0 FRACIMETVCGRPMTDDSEVSSSAQRTEVSSVSSSQVGPS* 0

>SWS1_anoCar Anolis carolinensis (lizard)
0 MSGQEDFYLFENISSVGPWDGPQYHIAPMWAFYFQTAFMGFVFFAGTPLNAIILIVTVKYKKLRQPLNYILVNISFAGFLFCTFSVFTVFMASSQGYFFFGRHVCAMEAFLGSVA 1
2 GLVTGWSLAFLAFERYIVICKPFGNFRFNSRHALLVVAATWIIGVGVAIPPFFGWSR 2
1 YIPEGLQCSCGPDWYTVGTKYKSEYYTWFLFIFCFIVPLTLIIFSYSQLLGALRA 0
0 VAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYASLAMYMVNNRDHGLDLRLVTIPAFFSKSSCVYNPIIYCFMNKQ 0
0 FRACILETVCGKPMSDESDVSSSAQKTEVSSVSSSQVSPS* 0

>SWS1_utaSta Uta stansburiana
0 MSGEEDFYLFENISSVGPWDGPQYHIAPMWAFYFQTAFMGFVFFAGTPLNAIILIVTVKYKKLRQPLNYILVNISFAGFLFCVFSVFTVFLASSQGYFFFGRHICALEAFLGSVA 1
2 GLVTGWSLAFLAFERYIVICKPFGNFRFNSKHALLVVAATWFIGIGVSIPPFFGWSR 2
1 FIPEGLQCSCGPDWYTVGTKYKSEYYTWFLFIFCFIVPLTLIIFSYSQLLGALRA 0
0 VAAQQQESATTQKAEREVSRMVVVMVGSFCLCYVPYAALAMYMVNNRDHGIDLRLVTIPAFFSKSACVYNPIIYCFMNKQ 0
0 FRACIMETVCGKPMTDESDVSSSAQKTEVSSVSSSQVSPS* 0

>SWS1_xenLae Xenopus laevis
0 MLEEEDFYLFKNVSNVSPFDGPQYHIAPKWAFTLQAIFMGMVFLIGTPLNFIVLLVTIKYKKLRQPLNYILVNITVGGFLMCIFSIFPVFVSSSQGYFFFGRIACSIDAFVGTLT 1
2 GLVTGWSLAFLAFERYIVICKPMGNFNFSSSHALAVVICTWIIGIVVSVPPFLGWSR 2
1 YMPEGLQCSCGPDWYTVGTKYRSEYYTWFIFIFCFVIPLSLICFSYGRLLGALRA 0
0 VAAQQQESASTQKAEREVSRMVIFMVGSFCLCYVPYAAMAMYMVTNRNHGLDLRLVTIPAFFSKSSCVYNPIIYSFMNKQ 0
0 FRGCIMETVCGRPMSDDSSVSSTSQRTEVSTVSSSQVSPA* 0

>SWS1_neoFor Neoceratodus forsteri (Australian lungfish)
0 MSGEEEFYLFKNISSVGPWDGPQYHIAPKWAFFLQAAFMGFVLFVGTPLNAIVLFVTIKYKKLQQPLNYILVNISLAGFIFCFFGVFAVFIASCQGYFIFGKTVCALEGFTGSVA 1
2 GLVTGWSLAILAFERYLVICKPIGNFRFGSKHSMIAVVAAWVIGVGVSIPPFFGWSR 2
1 YIPEGLQCSCGPDWYTVGTKYKSEYYTWFLFIFCFIIPLFIICFSYSQLLGALRA 0
0 VAAQQQESATTQKAEREVSRMIIVMVGSFCVCYVPYAALAMYMVNNRDHGIDLRLVTIPAFFSKSSFVYNPIIYCFMNKQ 0
0 FRACIMQTVFGKPMTDDSDISSSGKTEVSSVSSSQVNPS* 

>SWS1_danRer Danio rerio
0 MDAWAVQFGNASKVSPFEGEQYHIAPKWAFYLQAAFMGFVFIVGTPMNGIVLFVTMKYKKLRQPLNYILVNISLAGFIFDTFSVSQVSVCAARGYYSLGYTLCSMEAAMGSIA 1
2 GLVTGWSLAVLAFERYVVICKPFGSFKFGQGQAVGAVVFTWIIGTACATPPFFGWSR 2
1 YIPEGLGTACGPDWYTKSEEYNSESYTYFLLITCFMMPMTIIIFSYSQLLGALRA 0
0 VAAQQAESESTQKAEREVSRMVVVMVGSFVLCYAPYAVTAMYFANSDEPNKDYRLVAIPAFFSKSSSVYNPLIYAFMNKQ 0
0 FNACIMETVFGKKIDESSEVSSKTETSSVSA* 0

>SWS1_oryLat Oryzias latipes
0 MGKYFYLYENISKVGPYDGPQYYLAPTWAFYLQAAFMGFVFFVGTPLNFVVLLATAKYKKLRVPLNYILVNITFAGFIFVTFSVSQVFLASVRGYYFFGQTLCALEAAVGAVA 1
2 GLVTSWSLAVLSFERYLVICKPFGAFKFGSNHALAAVIFTWFMGVGCACPPFFGWSR 2
1 YIPEGLGCSCGPDWYTNCEEFSCASYSKFLLVTCFICPITIIIFSYSQLLGALRA 0
0 VAAQQAESASTQKAEKEVSRMIIVMVASFVTCYGPYALTAQYYAYSQDENKDYRLVTIPAFFSKSSCVYNPLIYAFMNKQ 0
0 FNGCIMEMVFGKKMEEASEVSSKTEVSTDS*0

>SWS1_geoAus Geotria australis lamprey UV-sensitive retinal pigment lamprey
0 MSGDEEFYLFKNISKVGPWDGPQFHIAPKWAFYLQAAFMGFVFICGTPLNAIVLVVTIKYKKLRQPLNYILVNISAAGLVFCLFSISTVFVASMQGYFFLGPTICALEAFFGSLA 1
2 GLVTGWSLAFLAAERYIVICKPFGNFRFGSKHALVAVGLTWMLGLSVALPPFFGWSR 2
1 YIPEGLQCSCGPDWYTVGTKYKSEYYTYFLFVFCFVVPLSIIIFSYGSLLGTLRA 0
0 VAAQQQESASTQKAEREVSRMVIMMVASFCTCYVPYAALAVYMVTNRDHNIDLRFVTVPAFFSKASCVYNPLIYSFMNKQ 0
0 FRACILETVCGKPITDESETSSSRTEVSSVSTTQMIPG* 0

>LWS_homSap OPN1MW NP_000504 length=364
0 MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTR 1
2 GPFEGPNYHIAPRWVYHLTSVWMIFVVIASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISVVNQVYGYFVLGHPMCVLEGYTVSLC 1
2 GITGLWSLAIISWERWMVVCKPFGNVRFDAKLAIVGIAFSWIWAAVWTAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCITPLSIIVLCYLQVWLAIRA 0
0 VAKQQKESESTQKAEKEVTRMVVVMVLAFCFCWGPYAFFACFAAANPGYPFHPLMAALPAFFAKSATIYNPVIYVFMNRQ 0
0 FRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA* 0

>LWS_monDom OPN1MW NP_000504 length=364 macEug for last exon
0 MTQAWDPAGFLARRRDVNEDDNDETTRSSLFVYTNSNNTR 1
2 GPFEGPNYHIAPRWVYNLTSLWMVFVVIASIFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETVIASTISVINQIYGYFILGHPLCVLEGYTVSLC 1
2 GITGLWSLAIISWERWVVVCKPFGNVKFDAKLAMVGIIFSWVWAAVWTAPPLFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMATCCIFPLSIILLCYVQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTLFACFAAANPGYSFHPLTASLPAYFAKSATIYNPIIYVFMNRQ 0
0 FRTCILQLFGKKVDDGSEVSSTSRTEVSSVSSVAPA* 0 

>LWS_ornAna platypus LWS green cone -Ultra401:228,567-238,486
0 MTPAWNSGVYAARRRFEDEEDTTRTSVFVYTNSNNTR 1
2 DPFEGPNYHIAPRWAYNVTSLWMIFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETLIASTISVINQIFGYFILGHPMCVLEGYTVSLC 1
2 GITGLWSLSIISWERWIVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSIIVLCYLQVWLAIRA 0
0 VAKQQKESESTQKAEKEVSRMVVVMILAYCFCWGPYTIFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ 0
0 FRNCIMQLFGKKVDDGSELSSTSRTEVSSVSSVSPA* 0

>LWS_galGal chicken green iodopsin red cone long-wave-sensitive missing in assembly
0 MAAWEAAFAARRRHEEEDTTRDSVFTYTNSNNTR 1
2 GPFEGPNYHIAPRWVYNLTSLWMIFVVAASVFTNGLVLVATWKFKKLRHPLNWILVNLAVADLGETVIASTISVINQISGYFILGHPMCVVEGYTVSAC 1
2 GITALWSLAIISWERWFVVCKPFGNIKFDGKLAVAGILFSWLWSCAWTAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMVVLMVTCCFFPLAIIILCYLQVSLAIRA 0
0 VAAQQKESESTQKAEKEVSRMVVVMIVAYCFCWGPYTFFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ 0
0 FRNCILQLFGKKVDDGSEVSTSRTEVSSVSNSSVSPA* 0

>LWS_anoCar Anolis
0 MAGTVTEAWDVAVFAARRRNDEDDTTRDSLFTYTNSNNTR 1
2 GPFEGPNYHIAPRWVYNITSVWMIFVVIASIFTNGLVLVATAKFKKLRHPLNWILVNLAIADLGETVIASTISVINQISGYFILGHPMCVLEGYTVSTC 1
2 GISALWSLAVISWERWVVVCKPFGNVKFDAKLAVAGIVFSWVWSAVWTAPPVFGWSR 2
1 YWPHGLKTSCGPDVFSGSDDPGVLSYMIVLMITCCFIPLAVILLCYLQVWLAIRA 0
0 VAAQQKESESTQKAEKEVSRMVVVMIIAYCFCWGPYTVFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ 0
0 FRNCIMQLFGKKVDDGSELSSTSRTEVSSVSNSSVSPA* 0

>LWS_xenTro Xenopus tropicalis (frog) NM_001102861
0 MASHWNEAVFAARRRNDDDDTTRSSVFTYTNSNNTR 1
2 GPFEGPNYHIAPRWVYNISSLWMIFVVLASVFTNGLVLVATLKFKKLRHPLNWILVNMAIADLGETVIASTISVCNQIFGYFVLGHPMCILEGYTVSVC 1
2 GIAALWSLTVIAWERWFVVCKPFGNIKFDGKLAATGIIFSWVWAAGWCAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMLVLMITCCIIPLAIIVLCYMHVWLTIRQ 0
0 VAQQQKESESTQKAEREVSRMVVVMIIAYIFCWGPYTFFACFAAFNPGYNFHPLAAAMPAYFAKSATIYNPIIYVFMNRQ 0
0 FRNCIYQLFGKKVDDGSEVSSTSRTEVSSVSNSSVSPA* 0

>LWS_neoFor Neoceratodus forsteri (Australian lungfish)
0 MAEPWDAVLAARRRHQDEETTRSTIFVYTNSNNTR 1
2 GPFEGPNYHIAPRWVYNLTSLWMIFVVFASCFTNGLVLMATYKFKKLRHPLNWILVNLAIADLGETLIASTISVTNQIFGYFILGHPMCMLEGFTVATC 1
2 GITGLWSLTIIAWERWVVVCKPFGNIKFDGKWAAGGIIFSWVWSAFWCAMPLFGWSR 2
1 FWPHGLKTSCGPDVFSGEDKYGTRSFMIALMITCCIIPLGVIILCYIQVWWAIRT 0
0 VAKQQKESESTQKAEKEVSRMVVVMIFAYCFCWGPYTFMACFGAAYPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ 0
0 FRNCIYQLLGKKVDDGSELSSTSKTEVSSVSNSSVSPA* 0

>LWS_takRub Takifugu rubripes (pufferfish)
0 MAEEWGKQSFAARRYHEDTTRGSAFVYTNSNHTR 1
2 DPFEGPNYHIAPRWVYNVATVWMFIVVVLSVFTNGLVLVATAKFKKLRHPLNWILVNLAIADLGETVFASTISVCNQFFGYFILGHPMCVFEGYTVSTC 1
2 GIAALWSLTIISWERWVVVCKPFGNVKFDAKWATGGIVFSWVWAAVWCAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSEDPGVQSYMIVLMITCCIIPLAIIILCYLAVWLAIRS 0
0 VAMQQKESESTQKAEKEVSRMVVVMIVAYCVCWGPYTFFACFAAANPGYAFHPLAAAMPAYFAKSATIYNPVIYVFMNRQ 0
0 FRVCIMKLFGKEVDDGSEVSTSKTEVSSVAPA* 0

>LWS_gasAcu Gasterosteus aculeatus
0 MAEEWGKQAFAARRYNEDTTRGSMFVYTNSNNTK 1
2 DPFEGPNYHIAPRWVYNLSTLWMFIVVALSVFTNGLVLVATAKFKKLQHPLNWILVNLAIADLGETVFASTISVCNQFFGYFILGHPMCVFEGYVVSVC 1
2 GITALWSLTIISWERWIVVCKPFGNVKFDAKWATAGIVFSWIWSAVWCAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSEDPGVQSYMIVLMITCCLIPLAIIILCYLAVWLAIRA 0
0 VAMQQKESESTQKAERDVSRMVVVMIVAYIVCWGPYTTFACFAAANPGYAFHPLAAAMPAYFAKSATIYNPVIYVFMNRQ 0
0 FRSCIMQLFGKEVDDGSEVSTsKTEVSSVAPA* 0

>LWS_petMar Petromyzon maritimus lamprey traces key to intron 3 position and gapping
0 MTASWQGAMFAARRRQDDEDTTMESLFRYTNENNTK 1
2 DPFEGPNYHIAPRWVFNLTSVWMIIVVVLSLFSNGLVLVATVKFKKLRHPLNWIIVNLAIADILETIFASTISVCNQVYGYFILGHPMCVFEGYVVSTC 1
2 GIAGLWSLAIISWERWMVVCKPFGNIKFDGKIATILIVFSWVWPASWCSLPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSTDPGVQSYMVVLMITCCFLPLSIIILCYLQVWLAIHS 0
0 VAQQQKESETTQKAERDVSRMVVVMILAYVFCWGPYTFFACFAAANPGYSFHPIAAALPAYFAKGATIYNPIIYVFMNRQ 0
0 FRNCILQLFGKKVDDGSEVSSSSRTEVSSVSNSSVSPA* 0

>LWS_letJap lamprey Lethenteron japonicum
0 MTASWHGAVFAARRRNDDEDTTKDSIFRYTNENNTR 1
2 DPFEGPNYHIAPRWMFNLTSVWMIIVVVLSLFTNGLVLVATMKFKKLRHPLNWILVNLAIADILETIFASTISVCNQVFGYFILGHPMCVFEGYVVSTC 1
2 GIAGLWSLAIISWERWMVVCKPFGNIKFDGKIAIILIVFSWVWPACWCSLPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSSDPGVQSYMVVLMVTCCFLPLSVIILCYLQVWLAIHS 0
0 VAQQQKESETTQKAERDVSRMVVVMILAYIFCWGPYTFFACYAAANPGYAFHPLTAALPAYFAKSATIYNPVIYVFMNRQ 0
0 FRNCIMQLFGKKVDDGSEVSSASRTEVSSVSNSSISPA*

>LWS_geoAus Geotria australis lamprey red-sensitive retinal pigment 
0 MAQSWERAMFAARRRQDEDTTKGDLFRYTNENNTR 1
2 DPFEGPNYHIAPRWMYNLTSFWMIIVVILSLFTNGLVLVATLKFKKLRHPLNWILVNLAIADIGETIFASTVSVVNQIFGYFILGHPLCVFEGFTVSVC 1
2 GITALWSLAIISFERWMVVCKPFGNLKFDGKVAIVLIIFSWAWSAGWCAPPIFGWSR 2
1 YWPHGLKTSCGPDVFSGSTDPGVQSYMVVLMITCCFIPLALIIICYLQVWLAIHT 0
0 VAQQQKESETTQKAERDVSRMVVVMIFAYIFCWGPYTFFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ 0
0 FRNCIMQLFGKKVDDGSEVSSSARTEVSSVSNSSVSPA* 0

>PIN_galGal pinopsin pineal pigment non-visual homSap- RPH3AL DOC2B PIN TEX14 RAD51C
0 MSSNSSQAPPNGTPGPFDGPQWPYQAPQSTYVGVAVLMGTVVACASVVNGLVIVVSICYKKLRSPLNYILVNLAVADLLVTLCGSSVSLSNNINGFFVFGRRMCELEGFMVSLT 1
2 GIVGLWSLAILALERYVVVCRPLGDFQFQRRHAVSGCAFTWGWALLWSTPPLLGWSSYVPE 1
2 GLRTSCGPNWYTGGSNNNSYILSLFVTCFVLPLSLILFSYTNLLLTLRA 0
0 AAAQQKEADTTQRAEREVTRMVIVMVMAFLLCWLPYSTFALVVATHKGIIIQPVLASLPSYFSKTATVYNPIIYVFMNKQ 0
0 FQSCLLEMLCCGYQPQRTGKASPGTPGPHADVTAAGLRNKVMPAHPV* 0

>PIN_utaSta Uta stansburiana pinopsin missing in Anole genome
0 MVNEWSNATPGPFDGPQWPYLAPRSIYTSVAVLMGLVVVSAAFVNGLVIVVSIQYKKLRSPLNYILVNLAIADLLVTSFGSTLSFANNIYGFFVLGQTACEFEGFMVSLT 1
2 GIVGLWSLAILAFERYLVICKPVGDFRFQQRHAVFGCVFTWMWSLVWTLPPLFGWSSYVPE 1
2 GLRTSCGPNWYTGGSGNNSYIMALFVTCFALPLGMIIFSYASLLLTLRA 0
0 VATQQKEVETTQQAEKEVTRRVIAMVMAFLVCWLPYASFAMVVATNKDLVIQPALASLPSYFSKTATVYNPIIYVFMNKQ 0
0 FRSCLLSTMSCGHRPRGAQETTPAMISIPQGPTSALQGSRNKVTPSASEGSGNEAIPS* 0

>PIN_pheMad Phelsuma madagascariensis gekko
0 MHVQMANASQASLKNGTLSPFDGPQWPHRASRRVYTSLAALMGVVVLSASLANGLVIAVSVRFKRLRSPLNYILVNLATADLLVTFFGSIISFVNNAVGFFVFGKTACRFEGFMVSLT 1
2 GIVGLWSLAILAFERYLVICKPVGDFQFQRRHAVIGCLYTWGWSLIWTVPPLFGWSSYVPE 1
2 GLGTSCGPNWYMGGTNNNSYIVALFVTCFALPLSMILFSYANLLLTLRA 0
0 VAAQQKEQETTQRAEKEVTRMVITMVMAFLVCWLPYATFAMVVATTKDLSIQPGLASLPSYFSKTATVYNPIIYVFMNKQ 0
0 FRSCLLNTVSCGRIPQTMPGTPATTAVRGGFVLTSEGRGNKVASTELHS* 0

>PIN_podSic Podarcis sicula pinopsin mRNA ruin lizard
0 MQASNASWVEVRNRTPGPFEGPQWPYLAPQSTYISVAVLMGLVVISATLVNGLVIVVSVQFKKLRSPLNYVLVNLAVADLLVTFFGSTISFVNNAQGFFIFGQATCEFEGFMVSLT 1
2 GIVGLWSLAILAFERYLVICKPVGDFRFPARHAVLGCAFTWGWSFVWTVPPLLGWSSYVPE 1
2 GLRTSCGPNWYSGGSSNNSYIMTLFVTCFAMPLSTILFSYANLLMTLRT 0
0 VAAQQKEQETTQRAEREVTRMVVAMVAAFLVCWLPYASFAMVVATHKDLAIRPALASLPSYFSKTATVYNPIIYVFMNKQ 0
0 FRSCLLYKMSCGHRALSSQDTTPAGISLPGRLTTSASKGSRNQVSPS* 0

>PIN_xenTro Xenopus tropicalis
0 MRAGNMSAYEAPGPYDGPQWPHLAPRSTFLTVAAVMCMVVILAFFVNGLVIVVTLKYKKLRSPLNYILVNLAIANLLVTIFGSSVSFSNNVVGYFFMGKTMCEFEGFMVSLT 1
2 GIVGLWSLAILAFERYLVICKPMGDFRFQQKHAILGCSFTWVWSFIWTSPPLFGWCSYVPE 1
2 GLRTSCGPNWYTGGTNNNSYIMALFLTCFIMPLSTIIFSYSNLLMALRA 0
0 VAAQQKDSETTQRAEKEVTRMVIAMVLAFLICWLPYASFAVVVAVNKDVVIEPTVASLPSYFSKTATVYNPIIYVFMNKQ 0
0 FRNCLMTLLCCGRSFGDDETSSASGRTDVTSVSEAGGNKVTPA* 0

>PIN_bufJap Bufo japonicus toad classifies somewhat oddly
0 MHSANMSALETPGPFEGPQWPHVAPRSTYLTVAVLMGMVVFLAFFVNGMVIVVSLKYKKLRSPLNYILVNLAVADILVTMFGSTVSFHNNIFGFFTLGKLVCELEGFVVSLT 1
2 GIVGLWSLAILAFERYIVICKPMGDFRFQQRHAVMGCAFTWIWAFLWTSPPLIGWCSYVPE 1
2 GLGTSCGPNWYTGGTNNNSYILALFTTCFMMPLTTIIFSYSNLLLALRA 0
0 VAAQQKESETTQRAEREVTRMVIAMVLAFLICWLPYAVFAIVMASNKNVVIDPTLASMPSYFSKTATVYNPVIYVFMNKQ 0
0 FRDCLTKLLCCGRNPFGEDETSTTSGRTDVTSVSEGGGNKVTPA* 0

>VAOP_galGal Gallus gallus exon 1 genbank error + too short + frameshift INPP5A NKX6 VAOP ALDH18A stubbed in terminal finch
0 MDVFRALGNESLLSNSSGPARWDPFHHPLDSIQPWHFRLVAAVMFVVTSLSLAENLAVILVTFKFKQLRQPVNYVIVNLSVADFLVSLTGGTISFLANLKGYFYMGHWACVLEGFAVTFF 1
2 GIVALWSLALLAFERYIVICRPVGNMRLRGKHAAQGIAFVWTFSFIWTIPPTMGWSSYTTSKIGTTCEPNW 2
1 YSGAYNDRSYIIAFFTTCFIVPLLVILVSYGKLLQKLRK 0
0 VSNTQGRLRTARKPERQVTRMVVVMIIAFLICWMPYAVFSILATAYPSIELDPHLAAIPAFFSKTATVYNPIIYVFMNKQ 0
0 FRMCLIQMFKCSAIETAESNMNPTSERATLTQDKRDSQLSVMAVRSTILKRKTGDEHRADDLWLFRQLQKPKCVPCRAGDGS* 0

>VAOP_anoCar Anole INPP5A NKX6 VAOP new
0 MAGLRREAENDSWLFDPSSSSAPFDPFLQPLDIIEPWNFHLISALMFVVTLFSLSENFTVILVTIKFKQLRQPLNYVIVNLSVADFLVSLIGGTISFSTNLKGYFYMGHWACVLEGFAVTFF 1
2 GIVALWSLALLAFERYVVICRPLGNMRLNGKHAALGVAFVWIFSFIWTVPPTMGWSSYTTSKIGTTCEPNW 2
1 YSGDYNDHTFIITFFTTCFILPLLVILVSYGKLMRKLRK 0
0 VSDTQGRLGTTRKPERQVTGMVVIMILAFLICWSPYAAFSILVTACPSIELDPRLAAIPAFFSKTATVYNPVIYVFMNNQ 0
0 FRKCLVQLFQCSSQETMDANVNPISEKDTLTHTKHCGEMSTVAAHVIVFNPRSEDEQGSCQSFAQLAISENKVYPL* 0

>VAOP_xenTro frog new lost synteny GSTO1 VAOP C10ORF92
0 MPTNVSLLATPENSTVWNPFTGPLKTIEAWNFHLLAALMFVVTSLSIAENFIVILVTAKFKQLRQPLNYIIVNLSVADFLVSVIGGTISIATNSRGYFYLGSWACVLEGFAVTFF 1
2 GIVALWSLSVLAFERYIVICRPLGNLRLQGKHSALAIIFVWVFSFVWTIPPTMGWSSYTTSKIGTTCEPNW 2
1 YSGEMRDHTYIITFLTTCFVFPLLVIFMSYGKLMRKLRK 0
0 VSDTQGRLGSTRKPEKEVTRMVVIMILAFLICWTPYAAFSILITAHPTIDLDPRLAAIPAFFAKTASMYNPIIYVYMNKQ 0
0 FRRCLYQMFNINDPEAKESNLNPTSERGVLTRNNNGGEMLAIATHITSSAVTNREEEKSSSNSFAHIPVSDNKVCPM*

>VAOP_danRer Danio rerio vertebrate ancient long opsin assembly missing exon 3 no synteny
0 MEASSAAVNAVSPAEDPFSAPLSSIAPWNYSVLAALMFVVTALSLSENFTVMLVTFRFQQLRQPLNYIIVNLSLADFLVSLTGGSISFLTNYHGYFFLGKWACVLEGFAVTFF 1
2 GIVALWSLAVLAFERFFVICRPLGNIRLRGKHAALGLVFVWSFSFIWTVPPVLGWSSYTVSRIGTTCEPNW 2
1 YSGNFHDHTFIITLFSTCFIFPLGVIIVCYCKLIRKLRK 0
0 VSNTHGRLGNARKPERQVTRMVVVMIVAFMVAWTPYAAFSIIITAHPSMHVDPRLAAIPAFVAKTAAVYNPIIYVFMNKQ 0
0 FRKCLVQLLSCSKVTVVEGNNNQTTERAGMTSGSNTGEMSAIAARVSVPKTEENPGDRSTFSHIPIPENKVCPM*

>VAOP_takRub Takifugu rubripes vertebrate ancient opsin
0 MESLSLSVNGVSYTVAAELAPTNDPFTGPINNIAQWNFTILAVLMFVVTSLSLCENFLVMFITFKFKQLRQPLNYIIVNLAIADFLVSLTGGLISFLTNARGYFFLGRWACVLEGFAVTYF 1
2 GIVAMWSLAVLSFERFFVICRPLGNMRLQAKHAAIGLLFVWTFSFVWTFPPVLGWNRYTVSKIGTTCEPDW 2
1 YSNNMTSHSYIITFFSTCFILPLGIIFFCYGKLLRKLRK 0
0 VSHGRLATARKPERQVTRMVVVMIVAFMVAWTPYATFAILVTIHPTIELDPR
0 FRKCLIQHFIGMGVMAESNMNPTSERPGITAESQTGEMSAIAARVPVGATAALHSDGSPTDCGSLAQLPIPENKVCPI* 0

>VAOP_rutRut Rutilus rutilus (roach minnow) vertebrate ancient opsin
0 MELFPVAVNGVSHAEDPFSGPLTFIAPWNYKVLATLMFVVTAASLSENFAVMLVTFRFTQLRKPLNYIIVNLSLADFLVSLTGGTISFLTNYHGYFFLGKWACVLEGFAVTYF 1
2 GIVALWSLAVLAFERFFVICRPLGNIRLRGKHAALGLLFVWTFSFIWTIPPVLGWSSYTVSKIGTTCEPNW 2
1 YSGNFHDHTFIIAFFITCFILPLGVIVVCYCKLIKKLRK 0
0 VSNTHGRLGNARKPERQVTRMVVVMIVAFMVAWTPYAAFSIVVTAHPSIHLDPRLAAAPAFFSKTAAVYNPVIYVFMNKQ 0
0 FRKCLVQLLRCRDVTIIEGNINQTSERQGMTNESHTGEMSTIASRIPKDGSIPEKTQEHPGERRSLAHIPIPENKVCPM* 0

>VAOP_petMar Petromyzon marinus 5 exons validated #123 in traces pineal gland-specific opsin AH006524
0 MDALQESPPSHHSLPSALPSATGGNGTVATMHNPFERPLEGIAPWNFTMLAALMGTITALSLGENFAVIVVTARFRQLRQPLNYVLVNLAAADLLVSAIGGSVSFFTNIKGYFFLGVHACVLEGFAVTYF 1
2 GVVALWSLALLAFERYFVICRPLGNFRLQSKHAVLGLAVVWVFSLACTLPPVLGWSSYRPSMIGTTCEPNW 2
1 YSGELHDHTFILMFFSTCFIFPLAVIFFSYGKLIQKLKK 0
0 ASETQRGLESTRRAEQQVTRMVVVMILAFLVCWMPYATFSIVVTACPTIHLDPLLAAVPAFFSKTATVYNPVIYIFMNKQ 0
0 FRDCFVQVLPCKGLKKVSATQTAGAQDTEHTASVNTQSPGNRHNIALAAGSLRFTGAVAPSPATGVVEPTMSAAGSMGAPPNKSTAPCQQQGQQQQQQGTPIPAITHVQPLLTHSESVSKICPV* 0

>PPIN_anoCar Anoles parapinopsin PPIN syntenic region deleted in chicken
0 MDSLDTNTLSPNASTVRVVLMPRIGYTIIAIIMATSCTLSVILNTAVIAITIKYRQLRQPINYSLVNLAIADLGAALLGGSLNVETNAVGYYNLGRVGCVTEGFAMAFF 1
2 GIVALCTIAVIAVDRAIVIAKPMGTITFTTRKAMIGVAVSWIWSLVWNTPPLFGWGGYQMEGVMTSCAPDWANSDPINVSYIICYFLFCFTIPFITILASYGYLIWTLRQ 0 
0 VAKVGLAQRGSTTKAEAQVSRMVIVMVMAFLICWLPYATFALVVVGNPQIYINPIIATIPMYMAKSSTFYNPIIYIFMNKQ 0
0 FRDCLVRCLLCGRNPCASEQTDEDDLEVSTIAPAPSSRRGKVAPV* 0

>PPIN_xenTro Xenopus tropicalis bistable UV pigment lamprey pineal broken contigs ACTR8 SELK PPIN CACNA2D3 genome only last 2
0 MADEALLPPMMNVTNEEMHPGKVLMPRIGYTILALIMAVFCAAALFLNVTVIVVTFKYRQLRHPINYSLVNLAIADLGVTVLGGALTVETNAVGYFNLGRVGCVIEGFAVAFF 1
2 GIAALCTIAVIALDRVFVVCKPMGTLTFTPKQALAGIAASWIWSLIWNTPPLFGWGSYELEGVMTSCAPNWYSADPVNMSYIVCYFSFCFAIPFLIIVGSYGYLMWTLRQ 0
0 VAKLGVAEGGTTSKAEVQVSRMVIVMILAFLVCWLPYAAFAMTVVANPGMHIDPIIATVPMYLTKTSTVYNPIIYIFMNKQ 0
0 FQECVIPFLFCGRNPWAAEKSSSMETSISVTSGTPTKRGQVAPA* 0

>PPIN_ictPun Ictalurus punctatus (channel catfish) parapinopsin index sequence
0 MASIILINFSETDTLHLGSVNDHIMPRIGYTILSIIMALSSTFGIILNMVVIIVTVRYKQLRQPLNYALVNLAVADLGCPVFGGLLTAVTNAMGYFSLGRVGCVLEGFAVAFF 1
2 GIAGLCSVAVIAVDRYMVVCRPLGAVMFQTKHALAGVVFSWVWSFIWNTPPLFGWGSYQLEGVMTSCAPNWYRRDPVNVSYILCYFMLCFALPFATIIFSYMHLLHTLWQ 0
0 VAKLQVADSGSTAKVEVQVARMVVIMVMAFLLTWLPYAAFALTVIIDSNIYINPVIGTIPAYLAKSSTVFNPIIYIFMNRQ 0
0 FRDYALPCLLCGKNPWAAKEGRDSDTNTLTTTVSKNTSVSPL* 0

>PPIN_danRer Danio rerio parapinopsin [not checked wgd or synteny]
0 MESETSTAASGSIAEVMPRMGYTILAVIIGVFSVCGVILNVTVITVTLKYKQLRQPLNFALVNLAVADLGCAVFGGLPTVVTNAMGYFSLGRVGCVLEGFAVAFF 1
2 GIAALCSVAVIALERCMVVCRPVGSISFQTRHAVFGVAVSWLWSFIWNTPPLFGWGRLQLEGVRTSCAPDWYSRDLANVSFIVCYFLLCFALPFSVIVYSYTRLLWTLRQ 0
0 VSRLQVCEGGSAARAEAQVSCMVVVMILAFLLTWLPYASFALCVILIPELYIDPVIATVPMYLTKSSTVFNPIIYIFMNRQ 0
0 FRDRALPFLLCGRNPWAAEAEEEEEETTVSSVSRSTSVSPA* 0

>PPIN_oncMyk Oncorhynchus mykiss
0 MDHQQLLPNLHGNISSSPGSVSEALLSRTGFTILAVIIGVFSVSGVCMNVLVIMVTMRHRKLRQPLNYALVNLAVADLGCALFGGLPTMVTNAMGYFSMGRLGCVLEGFAVAFF 1
2 GIAGLCSVAVIAVDRYVVVCRPMGAVMFQTRHAVGGVVLSWVWSFLWNTPPLFGWGSFELEGVRTSCSPNWYSREPGNMSYIILYFLLCFAIPFSIIMVSYARILFTLHQ 0
0 VSKLKVLEGNSTTRVEIQVVRMVVVMVMAFLLSWLPYAAFALSVILDPSLHINPLIATVPMYLAKSSTVYNPIIYVFMNRQ 0
0 FRDCAVPFLLCGLNPWASEPVGSEADTALSSVSKNPRVSPQ*

>PPIN_petMar Petromyzon maritimus lamprey trace-validated introns bistable pineal UV/green 
0 MENLTSLDLLPNGEVPLMPRYGFTILAVIMAVFTLASLVLNSTVIIVTLRHRQLRHPLNFSLVNLAVADLGVTVFGASLVVETNAVGYFNLGRVGCVIEGFAVAFF 1
2 GIAALCTIAVIAVDRFVVVCKPLGTLMFTRRHALLGITWAWLWSFVWNTPPLFGWGSYKLEGVRTSCAPDWYSRDPANVSYIVSFFSFCFAIPFLVIVVAYGRLLWTLHQ 0
0 VAKLGMGESGSTAKAEAQVSRMVVVMVVAFLVCWLPYALFAMIVVAKPGVYIDPVIATLPMYLTKTSTVYNPIIYIFMNRQ 0
0 FRDCAVPFLLCGRNPWAEPSSESATTASTSATSVTLASVPGQVSPS* 0

>PPIN_letJap Lethenteron japonicum lamprey AB116380 bistable pineal UV/green aligns perfectly
0 MENLTSLDLLPNGEVPLMPRYGFTILAVIMAVFTIASLVLNSTVVIVTLRHRQLRHPLNFSLVNLAVADLGVTVFGASLVVETNAVGYFNLGRVGCVIEGFAVAFF 1
2 GIAALCTIAVIAVDRFVVVCKPLGTLMFTRRHALLGIAWAWLWSFVWNTPPLFGWGSYELEGVRTSCAPDWYSRDPANVSYITSYFAFCFAIPFLVIVVAYGRLMWTLHQ 0
0 VAKLGMGESGSTAKAEAQVSRMVVVMVVAFLVCWLPYALFAMIVVTKPDVYIDPVIATLPMYLTKTSTVYNPIIYIFMNRQ 0
0 FRDCAVPFLLCGRNPWAEPSSESATAASTSATSVTLASAPGQVSPS* 0

>PPINa_cioInt Ciona intestinalis parapinopsin 8 mostly odd exons 88 +- 200671 202490 1820 opsin1 larval ocellus
0 MNQWLETIMNERKFEIVNSSLQG 2
1 LLKPDSLGMDHDVTPTVDLTDGVPQCKDLNPYVLKGDGWVPQHISRANRSTYSFLCVYMTFVFLLSCSLNILVIVATLKNK 0
0 VLRQPLNYIIVNLAVVDLLSGFVGGFISIAANGAGYFFWGKTMCQIEGYFVSNFGVTGLL 0
0 SIAVMAFERYFVICKPFGPVRFEEKHSIFGIV 0
0 ITWVWSMFWNTPPLIFWDGYDTEGLGTSCAPNWFVKEKRERLFIILYFVFCFVIPLAVIMICYGKLILTLRQ 0
0 IAKESSLSGGTSPEGEVTKMVVVMVTAFVFCWLPYAAFAMYNVVNPEAQ 0
0 IDYALGAAPAFFAKTATIYNPLIYIGLNRQ 0
0 FRDCVVRMIFNGRNPWVDELVGSQVSSTGSQLTAVSSNKVAPA* 0

>PPINb_cioInt Ciona intestinalis 137 +- 67340 71147 3808 new 61% classifies perhaps as PIN jgi gene model wrong both ends
0 MTTAETTTECYEKNPYIRNEMGWVPKHILIAERHIYTILAVYMTFIFLLAVSLNGFVIIATMKNK 0
0 KLRQPLNYIIINLSIADFLSGLVGGFIGMISNSAGYFYFGKTVCILEGYIVSVA 1
2 GVCGLMSISVMAFERYFVVCKPYGPFTLTNTHAAL 1
2 GIGFTWTWSVLWSTPGLIWLDGYVPEGLGTSCAPNWFSKNK 2
1 SERIFIFVYFVFCFFIPLLVIIICYGKIVLFLKQVSLY 0
0 ATRQSSASSNRQADNKVTKMVLVMISAFLICWTPYGVLSLYNAINPDKQ 0
0 LDYGLGAVPVFFAKTANIYNPLIYIGLNKQ 0
0 FRDGVIKMVFRGRNPWAEEMSTQQRQRSTEAGQPIVSNEV* 0

>PARIE_utaSta Uta stansburiana lizard ancestral shift in counterion gusducin and Go
0 MENDSSLATELAEGAIVKPTIFPKAGYGVLAFLMFLNALFSIFNNSLVIAVTLKNPQLRNPINIFILNLSFSDLMMSLCGTTIVIATNYYGYFYLGRKFCIFQGFAVNYF 1
2 GIVSLWSLTILAYERYNVVCQPLGTLQMSTKRGYQLLGFIWVFCLFWAVVPLFGWSSYGPEGVQTSCSIGWEERSWSNYSYLIVYFLSCFFIPVLIIGFSYGNVIRSLHG 0
0 LNKKVEQLGGKSSPEEEFRAVIMVLVMVVAFLICWLPYTVFALIVVFNPALNISPLAATIPTYLSKTSPVYNPIIYIFLNKQ 0
0 FRDCAVEFITCGQVVLTSPEEDISTSAIPVEGKGPCKINQVTPV* 0

>PARIE_anoCar Anole carolinensis KERA DCN BTG1 PARIE EEA1 NUDT4
0 MENESSLVLEGAEGYIVRPTIFPRAGYGVLAFLMFINALFSLFNNFLVIAVTLKNPQLRNPINIFILNLSFSDLMMSICGTTIVIATNYHGYFYLGRRFCIFQGFAVNYF 1
2 GIVSLWSLTILAYERYNVVCQPLGTLQMSTQRAYQLLGFIWVFCLFWAVVPLFGWSSYGPEGVQTSCSIGWEERSWNNYSYLIVYFLSCFFIPVLIIGFSYGNVIRSLHG 0
0 LNKKVEQLGGKSNPEEEFRAVIMVLVMVVAFLICWLPYTLFALTVVFNPALNISPLAATIPTYLSKTSPVYNPIIYIFLNKE 0
0 FRECAVEFITCGKVVLTSPEEDISTSAISDEGIAPCKINQVTPV* 0

>PARIE_xenTro Xenopus tropicalis parietopsin
0 MDGNSTTPGIAVNLTVMPTIFPRSGYSILSFLMFLNAVFSICNNAIVILVTLKHPQLRNPINIFILNLSFSDLMMALCGTTIVVSTNYHGYFYLGKQFCIFQGFAVNYF 1
2 GIVSLWSLTLLAYERYNVVCEPIGALKLSTKRGYQGLVFIWLFCLFWAIAPLFGWSSYGPEGVQTSCSIGWEERSWSNYSYIISYFLTCFIIPVGIIGFSYGSILRSLHQ 0
0 LNRKIEQQGGKTNPREEKRVVIMVLFMVLAFLICWLPYTVFALIVVINPQLYISPLAATLPTYFAKTSPVYNPIIYIFLNKQ 0
0 FRTYAVQCLTCGHINLDSLEEDTESVSAQAENMLTPKTNQVAPA* 0

>PARIE_takRub Takifugu rubripes (teleost)
0 MDSNSTPWSSPPAPLQAEAVTVAPTIFPRVGYSILSFLMFINTVLSVFNNSLAIAVMLKNPSLLQPINIFILSLAVSDLMIGLCGSLVVTITNYHGSFFIGHTACVFQGFAVNYF 1
2 GLVSLCTLTLLAYERYNVVCKPRAGLKLTMRRSIIGLLFVWTFCLFWAVTPLLGWSSYGPEGVQTSCSLAWEERSWNNYSYLILYTLLCFIFPVGVIIYCYCKVLTSMNK 0
0 LNKSVELQGGLSCRRENKHAINMVLAMIIAFFVCWLPYTALSVVVVVDPELHIPPLVATMPMYFAKTSPVYNPIIYFLSNKQ 0
0 FRDATLEVLSCSRYIPHASSRVSINMRSLNRRSVNTHSKVSPL* 0

>PARIE_gasAcu Gasterosteus aculeatus (stickleback)
0 MDSNSTLWSSGSPPPSIHGKMLTITPTIFPRVGYSILSFLMFINTVLTVFNNVLVITVLVRNPSLLQPMNVFILSLAVSDLMIGLCGSLVVTITNYHGSFFIGHTACIFQGFAVNYF 1
2 GLVSLCTLTLLSYERYNVVCRPRNALKLSMRRSIHGLLIVWTFCLFWAVAPLFGWSGYGPEGVQTSCSLAWEERSWSNYSYLVLYTLLCFIVPVAVIIYCYAKVLTSMNT 0
0 LNRSVEVQGGRSSQKENDHAVSMVLAMIIAFFSCWLPYTALSVVVVVDPTLYIPPLVATMPMYFAKTSPVYNPIIYFLSNKQ 0
0 FRDAALEMLSCGRYIAHMPNTVSINMRSLNRRSRLSSLSRNVNSHSKVLPL* 0

>PARIE_danRer Danio rerio 
0 MENFAKTELTMMVQPTIFPRVGYSILSYLMFINTTLSVFNNVLVIAVMVKNLHFLNAMTVIIFSLAVSDLLIATCGSAIVTVTNYEGSFFLGDAFCVFQGFAVNYF 1
2 GLVSLCTLTLLAYERYNVVCKPMAGFKLNVGRSCQGLLLVWLYCLFWAVAPLLGWSSYGPEGVQTSCSLGWEERSWRNYSYLILYTLMCFILPTVIITYCYSNVLLTMRK 0
0 INKSIECQGGKNCAEDNEHAVRMVLAMIIAFFICWLPYTAISVLVVVNPEISIPPLIATMPMYFAKTSPVYNPIIYFLTNKR 0
0 FRESSLEVLSCGRYISRETGGPLMGSSMQRGQSRVNPV* 0

>ENCEPH_homSap encephalopsin OPN3 panopsin encephalopsin 1q43 - 47250 4 chr1:239,823,075-239,870,324 402 cone opsin with intron loss
0 MYSGNRSGGHGYWDGGGAAGAEGPAPAGTLSPAPLFSPGTYERLALLLGSIGLLGVGNNLLVLVLYYKFQRLRTPTHLLLVNISLSDLLVSLFGVTFTFVSCLRNGWVWDTVGCVWDGFSGSLF 1
2 GIVSIATLTVLAYERYIRVVHARVINFSWAWRAITYIWLYSLAWAGAPLLGWNRYILDVHGLGCTVDWKSKDANDSSFVLFLFLGCLVVPLGVIAHCYGHILYSIRM 0
0 LRCVEDLQTIQVIKILKYEKKLAKMCFLMIFTFLVCWMPYIVICFLVVNGHGHLVTPTISIVSYLFAKSNTVYNPVIYVFMIRK 0
0 FRRSLLQLLCLRLLRCQRPAKDLPAAGSEMQIRPIVMSQKDGDRPKKKVTFNSSSIIFIITSDESLSVDDSDKTNGSKVDVIQVRPL* 0

>ENCEPH_monDom encephalopsin Monodelphis domestica 82% funny business with extra intron, alt splicing
0 MYSDNSSDDGGGGYWGSGRAGGASGTGVTGEPGPEGSPRQAPLFSPGTYELLALLIATIGLLGLCNNLLVLVLYYKFQRLRTPTHLFLVNISFNDLLVSLFGVTFTFVSCLRSGWVWDSVGCAWDGFSNTLF 1
2 GIVSIMTLTVLAYERYNRIVHAKVINFSWAWRAITYIWLYSLVWTGAPLLGWNRYTLEIHGLGCSVDWKSKDPNDSSFVIFLFFGCLMLPVGVMAYCYGHILYAIRM
0 LRCVEELQTIQVIKILRYEKKVAKMCFLMIAIFLFCWMPYAVICLLVANGYGSLVTPTVAIIASLFAKSSTAYNPIIYIFMSRK 0
0 FRRCLLQLLCFRLLKFQQPKKDRPVIRTEKQIRPIVMSQKVGDRPKKKVTFSSSSIIFIITSDETQMIDENDKNSGTKVNVIQVRPL* 0

>ENCEPH_galGal chicken encephalopsin
0 MHSGNGTGATSRPQLAAAGHEVPGERPLFSAGTYELLALLIATIGTLGVCNNLLVLVLYYKFKRLRTPTNLFLVNISLSDLLVSVCGVSLTFMSCLRSRWVWDAAGCVWDGFSNSLF 1
2 GIVSIMTLTVLAYERYIRVVHAKVIDFSWSWRAITYIWLYSLAWTGAPLLGWNRYTLEIHGLGCSMDWKSKDPNDTSFVLLFFLGCLVAPVVIMAYCYGHILYAVRM 0
0 LRCVEDFQTSQVIKLLKYEKKVAKMCFLMISTFLICWMPYAVVSLLVTYGYSNLVTPTVAIIPSFFAKSSTAYNPVIYIFMSRK 0
0 FRQCLLQLLCFRLMRFQRIMKEPSGAGNVKPIRPIVMSQKVGDRPKKKVTFSSSSIIFIIASDDTQQIDDNSKHNGTKVNVIQVKPL* 0

>ENCEPH_anoCar lizard encephalopsin
0 MFSANGTRSGAGSDLEPGPGQQQQQREASEEEERGAGLSPFSAGTYELLALLVAAIGLLGLCNNLLVLVLYAKFKRLRTPTHLFLVNISLSDLLVSLFGVSFTFGSCLRHRWVWDAAGCVWDGFSNSLF 1
2 GIVSIMTLTVLAYERYIRVVHARVIDFSWSWRAITYIWLYSLAWTGAPLLGWNHYTLEIHGLGCSVDWQSKEPSDSSFVLFFFLGCLAAPVGIMAYCYGHILHAIRM 0
0 LRCVEDLQSIQVIKILRYEKKVAKMCFLMVTTFLICWMPYAVVSLLIAYGYGHLITPTVAIIPSFFAKSSTAYNPVIYIFMSRK 0
0 FRRCLVQLFCVQFLRFKRTLKEQPAIESNKPIRPIVMSQKVGDRPKKKVTFSSSSIIFIITSDDTEQIDVSTKCSDTKINVIQVKPL* 0

>ENCEPH_xenTro Xenopus frog encephalopsin called teleost multiple tissue TMT opsin in fish
0 MPVTNGSHNNSISWLHSKDMFTEDTYHFLALIVATVGFLGLVNNLLVLILYCKFKRLQTPTNLLFFNTSLCHFVFSLLAITFTFMSCVRGSWAFSVEMCVFHGFSKNLL 1
2 GIVSFGTLTVVAYERYARVVYGKYVNSSWSKRSITFVWVYSLAWTGFPLIGWNLYTFETHKLDCSFEWTATDPKDTAFVLLFFLACITLPLSIMAYCYGYILYEIQK 0
0 LRSVKNIQNFQEITILDYEIKMAKMCLLMMLTFLIGWMPYTILSLLVTSGYSKFITPTITVMPSLLAIASAAYNPVIHIFTIKK 0
0 FRQCLVQLLPPINFHPPINPPINNFWRLLKNLNGRLAMKKVKPVLGKGRSHNRPEKKVPPINFSSSDFFTRTTSDTGTHGITESTKGKRTNVRLIQVHPL* 0

>ENCEPH4a_takRub Takifugu rubripes length=403 AF402774 multiple tissue opsin organs and cell lines exhibit circadian oscillations in clock gene 
0 MIVSNVSLSGCAGVNGAVCAAEGHQAGGSDRSTLTPTGNLVVSVFLGFIGTFGLVNNLLVLVLFCRYKMLRSPINLLLMNISISDLLVCVLGTPFSFAASTQGRWLIGEAGCVWYGFANSLF 1
2 GVVSLISLAVLSFERYSTMMTPTEADPSNYCKVCLGITLSWVYSLVWTVPPLFGWSSYGPEGPGTTCSVNWTAKTTNSISYIICLFVFCLIVPFLVIVFCYGKLLCAIRQ 0
0 VSGINASTSRKREQRVLCMVVIMVICYLLCWLPYGVVALLATFGPPDLVTPEASIIPSVLAKSSTVINPIIYVFMNKQ 0
0 FYRCFLALLCCQDPRSGSSMKSSSKVATKAKGVTPTGQRRTDFLYMVASLGRPAATIPQLGPSFDATNDFTKPPSSDTIKPVVVSLAAHCDG*

>ENCEPH4b_takRub Takifugu rubripes length=342 64% +CES1 +ENC
0 MIVCNVSLSCAHCPGEGTAANDAYAQASGSLATPTLSQRGHLVVAVCLGFIGTVGFLSNFLVLALFCRYRALRTPMNLMLVSISASDLLVSVLGTPFSFAASTQGRWLIGRAGCVWYGFVNACL 1
2 GIVSLISLAVLSYERYCTMVSSTIASNRDYRPVLGGICFSWFYSLAWTVPPLLGWSRYGPEGPGTTCSVDWRTQTPNNISYIVCLFTFCLLLPFFVILYSYGKLLHTIRQ 0 
0 VRRVSSTVTRRREHRVLVMVVAMVVCYLICWLPYGVTALLATFGPPNLLTPEATITPSLLAKFSTVINPFIYIFMNKQ 0
0 FYRCFRAFLNCSTPKRDSTVRTFTRISLRALRQDQQQKGSALAPSSARPTPNSIHESSLKGSHSTPSNGGAAAAKSPAANRSKPKLILVAHYRE* 0

>ENCEPH_takRub Takifugu rubripes encephalopsin syntenic to mammal
0 MNPANGSRSERSAEQLLFSGDTYRVLAFTIGTIGAFGFCNNFVVLALYCRFKRLRTPTNLLLVNISLSDLLVSLFGINFTFAACVQGRWTWTQATCVWDGFSNSLF 1
2 GIVSIMTLAALAYERYIRVVHAQVVDFPWAWRAIGHIWLYALAWTGAPLLGWNRYTLEIHRLGCSLDWASKDPNDASFILLFLLACFFVPVGIMIYCYGNILYAVQM 0
0 IRSIQDLQTVQIIKILRYEKKVSVMFFLMISCFLLCWTPYAVVSMMVAFGRRSMVSPTMAIIPSFFAKSSTAYNPLIYVFMSRK 0
0 FRHCLLQLLCSRLSWLQRSLKERPLAPVQRPIRPIVMSRPCGKGNRPKKKVTFSSSSIVFIITSDDFGQLDVTSKSGDSADVNAIQVRPL* 0

>ENCEPH_gasAcu Gasterosteus aculeatus (stickleback) encephalopsin
0 MNPDNGTREERSTDHSIFAVGTYKLLAFAIGTIGVFGFCNNVVVIVLYCKFKRLRTPTNLLVVNISLSDLLVSVIGINFTFVSCIRGGWTWSRATCIWDGFSNSLF 1
2 GIVSIMTLASLAYERYIRVVHAQVVDFPWAWRAIGHIWLYSLVWTGAPLLGWNRYTLEIHRLGCSLDWASKDPNDASFILLFLLACFFVPVGIMIYCYGNILYAVQM 0
0 LRSIQDLQTVQIIKILRYEKKVAVMFLLMISCFLLCWTPYAVVSMMEAFGRKNMVSPTVAIIPSFFAKSSTAYNPLICVFMSRK 0
0 FRRCLMQLLCSRVTCLQCNLKERPLAPVQRPIRPIVVSAACGGGRVRPKKRVTFSSSSIVFIITRNDIRHTDVTSNTRESSEANVFQVRPL* 0

>ENCEPH4_braFlo Branchiostoma floridae Amphiop4 AB050608 Un - 207841137 207851157 10021 new exon12 and 34 + perfect fit +CES1 -ENC -RTF1 TMT-opsin takRub
0 MALYNNTSSPSQDLLWDAPYSQGHIWDNSSASNSSEDVMDQGKVELQDFSDAGYTAIATCLALI 1
2 GFVGFTNNFVVILLIGCHRQLRTPFNLLLLNMSVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANSLF 1
2 GIVSLVTLSALAFERYCVVVRSSDMLTYKSSLVVITFIWLYSLLWTSLPLLGWSSYQFEGHN 0
0 VGCSVNWVQHNPDNVSYIVTLMVTCFFVPMVVVCWSYAWIWRTVRM 0
0 SSEAKPECGNSQNAGRLVTTMVVVMIICFLVCWTPYAVMALIVTFGADHLVTPTASVIPSLVAKSSTAYNPIIYVLMNNQ 0
0 FREFLLARLQRVCCRQQAVPRVTPMDDNVHVRLGGEGPSQSQQFLPAGENVENVDMLEYVQENCKPKADSLSTISE* 0

>ENCEPH4_braBel Branchiostoma belcheri Amphiop4 pinopsin encephalopsin introns from braFlo 83%
0 MPLYNTSSGPTQGLPWDTPYSQDPIWNDSSPSNSSEDAVVDQGRGELQDFSDAGYTAIATGLALI 1
2 GLVGSMNNFVVILLIGCHRQLRTPFNLLLLNVSVADLLVSVCGNTLSFASAVQHRWLWGRPGCVWYGFANSLF 1
2 GIVSLVTLSALAFERYCVVVRSSEMLTYKSSLGMIAFIWMYSLLWTSLPLLGWSSYQFEGHS 0
0 VGCSVNWVKHNVNNVSYIITLMVTCFFVPMVVVCWSYACIWRTVRM 0
0 SAEMKSEFGNPQNTGRLVTTMVVVMIVCFLVCWTPYTVMALIVTFGADHLVTPTASVIPSLVAKSSTAYNPIIYVLMNNQ 0
0 FREFLLARLRTFCCRQPRMLRVTPMDDNAHARLVGEGPSHAQQVIPSEENGENVEMRKVQGNQLKADSLSTISE* 0

>ENCEPH5_braFlo Branchiostoma floridae extra 00 intron chrUn:398,023,923-398,103,839 +ETF1 -ENC -RTF1
0 MLGMHNVMNATDYDNNNATFAAWNFQRNGTTEEEVEFSGFDTVAVVIAAIGIAGFLSNGAVVLLFLKFRQLRTPFNMLLLNMSVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANHLF 1
2 GLVSLISLAVISYERYRMVVKPKGPGSSYLTYNKVGLAIIFIYLYCLLWTTLPIVGWSSYQLE 0
0 GPKISCSVAWEEHSLSNTSYIVAIFIMCLLLPLLIIIYSYCRLWYKVKK 0
0 GSQNLPPAIRKSSQKEQKIARMVVVMITCFLVCWLPYGAMALVVSFGGESLISPTAAVVPSLLAKSSTCYNPLVYFAMNNQ 0
0 FRRYFQDLLCCGRRLFDASASVNTCNTSAMPRHSPVFQKPDSDQYNGIQKSREPQMRTTGQNAPYRQWIEMQTIAVVVKADEVNNKFGEVKT* 0

>ENCEPH5_braBel Branchiostoma belcheri Amphiop5 AB050609 early dup of ENCEPH extra Nfrag in mrna
0 MLGIYNVVNATEYGNNTTFAAWDFKRNGTGGEEEVEFFGYDAVAGVIAIIGVVGFVSNGAVVVLFLKFPQLRTPFNLLLLNMAVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANHLF 1
2 GLVSLISLAVISFLRYRMVVKPKGPGSSYLTYTKVGLAILFIYLYCLLWTTLPIAGWSSYQLE 0
0 GPKIGCSVAWEEHSWSNTSYIVVLFITCLFAPLLIIVYSYYRLWHKVKQ 0
0 GSRNLPAAMRKSSQKEQKIAMMVIVMITCFMVCWLPYGAMALVVTFGGERLISHTAAVVPSLLAKSSTCYNPVVYFAMNSQ 0
0 FRRYFQDLLCCGRRLFDVSQSVVTGNTAMPRNNSQGFRKDDSDQKQDNGLPKQSEGPMCDHSSNESQMEGSRHNTAASQQWIEMQTIAVVVKAVEVDTSAANEP* 0

>ENCEPH_apiMel Apis mellifera (honey bee) NM_001039968 328 aa ciliary opsin Gt pteropsin clock -ENC -PSMA3
0 MSLNRSTMEHVIYEDQVSPVMYIGAAIALGFIGFFGFTANLLVAIVIVKDAQILWTPVNVILFNLV 0
0 FGDFLVSIFGNPVAMVSAATGGWYWGYKMCLW 2
1 YAWFMSTLGFASIGNLTVMAVERWLLVARPMQALSIR 2
1 HAVILASFVWIYALSLSLPPLFGWGSYGPEAGNVSCSVSWEVHDPVTNSDTYIGFLFVLGLIVPVFTIVSSYAAIVLTLKKVRKRA 1
2 GASGRREAKITKMVALMITAFLLAWSPYAALAIAAQYFN 0
0 AKPSATVAVLPALLAKSSICYNPIIYAGLNNQFSRFLKKIFDARGSRTAVPDSQHTALTALNRQEQRK* 0

>ENCEPH1_anoGam Anopheles gambiae XM_312503 GPROP11 adjacent head-to-head tandem GPROP12
0 MYDVTDAAAINSDHQELMAPWAYNGAAVTLFFIGFFGFFLNIFVIALMYKDVQ 0
0 LWTPMNIILFNLVCSDFSVSIIGNPLTLTSAISHRWLYGKSICVAYGFFMSLL 1
2 GIASITTLTVLSYERFCLISRPFAAQNRSKQGACLAVLFIWSYSFALTSPPLFGWGAYVNEAANI 1
2 SCSVNWESQTANATSYIIFLFIFGLILPLAVIIYSYINIVLEMRK 0
0 NSARVGRVNRAERRVTSMVAVMIVAFMVAWTPYAIFALIEQFGPPELIGPGLAVLPALVAKSSICYNPIIYVGMNTQ FRAAFWRIRRSNGVAGQPDSNNTNNSNRDKESARHTAKEGL
ECSLDFCHWTVRGTRVSISSAERNVPAPAARERSGGHSVTGSREESRDRHVTLKTMLSVGPRSPSSVAPVAADCSTTDVPTSGDGSVRIVRQDSELSVIHDGGGGGGGSSSRVLVIKSQKPRSNML* 0

>ENCEPH2_anoGam Anopheles gambiae XM_312502 GPROP12
0 MNDAPNDVAASAVDYEDLMAPWAYNASAVTLFFIGFFGFFLNLFVIALMCKDMQ 0
0 LWTPMNIILFNLVCSDFSVSIIGNPLTLTSAISHRWIFGRTLCVAYGFFMSLL 1
2 GITSITTLTVLSYERYCLISRPFSSRNLTRRGAFLAIFFIWGYSFALTSPPLFGWGAYVQEAANI 1
2 SCSVNWESQTKNATTYIIFLFVFGLVVPLIVIVYSYTNIIVNMRE 0
0 NSARVGRINRAEQRVTSMVAVMIVAFMVAWTPYAIFALIEQFGPPELIGPGLAVLPALVAKSSICYNPIIYVGMNTQ FRAAFSRVRNKGQQAAADQNTTTMQRELTKSSRDMVECSF
DFCRKKSRFKISLVKPTAPLAVVDVSSTSHRDKGTSRSPLDQTVLNETNEDVGRERSGGGGGGGAYAGTRFVRPDFELSVINSGKSILIKSKNFRSNLL* 0

>CILL2_plaDum Platynereis dumerilii CT030681 htgs 171779 bp new CILL1 ENCEPH PARIE 44% e-65 393 5 exons 1 missing
0 MDDLGFLGNSSVNYTVPLLQEDPLLLRILYFGPTSYVITAIYLCIVGVIGTLSNGVIMYLYFKDKSLRSPMNLLFVNLAMSDFTVAFFGAMFQFGLTCTRKYMSPGMALCDFYGFITFLG 1
2 GLASEMNLFIISVERYLAVVRPFDVGNLTNRRVIAGG 1
2 VFVWLYSLVFAGGPLVGWSSYRPEGLGTWCSISWQDRSMNTMSYVTAVFLGCYFFPVSIIIFCYFNVWRKVKE 0
0 AADAQGAGTAGKAEKSIFRMSVIMVTCYLTAWTPYAIVCLIASYGPPNGLPIYAEVLPSLFAKSSQVYNPIIYVLMNKP 0
0 0* 0

>CILL1_plaDum Platynereis dumerilii AAV63834 Gt lophotrochozoa ciliary opsin mRNA polychaeta unavailable genomically
0 MDGENLTIPNPVTELMDTPINSTYFQNLNAETDGGNHYIYNAFTATDYNICAAYLFFIACLGVSLNVLVLVLFIKDRKLRSPNNFLYVSLALGDLLVAVFGTAFKFIITARKTLLREEDGFCKWYGFITYLG 1
2 GLAALMTLSVIAFVRCLAVLRLGSFTGLTTRMGVAAMA 1
2 FIWIYSLAFTLAPLLGWNHYIPEGLATWCSIDWLSDETSDKSYVFAIFIFCFLVPVLIIVVSYGLIYDKVRK 0
0 VAKTGGSVAKAEREVLRMTLLMVSLFMLAWSPYAVICMLASFGPKDLLHPVATVIPAMFAKSSTMYNPLIYVFMNKQ 0
0 FRRSLKVLLGMGVEDLNSESERATGGTATNQVAAT*

>RGR_homSap 10q23.1 + 14136 7 chr10:85,994,789-86,008,924 295 exons distinct retinal pigment epithelium and Mueller cells
0 MAETSALPTGFGELEVLAVGMVLLVE 1
2 ALSGLSLNTLTIFSFCKTPELRTPCHLLVLSLALADSGISLNALVAATSSLLR 2
1 VSHRRWPYGSDGCQAHGFQGFVTALASICSSAAIAWGRYHHYCT 1
2 RSQLAWNSAVSLVLFVWLSSAFWAALPLLGWGHYDYEPLGTCCTLDYSKGDR 2
1 NFTSFLFTMSFFNFAMPLFITITSYSLMEQKLGKSGHLQ 0
0 VNTTLPARTLLLGWGPYAILYLYAVIADVTSISPKLQM 0
0 VPALIAKMVPTINAINYALGNEMVCRGIWQCLSPQKREKDRTK* 0

>RGR_galGal Gallus gallus (chicken) Opsin photoisomerases in the chick retina and pineal gland 14985289 NM_001031216
0 MVTSHPLPEGFTEIEVFAIGTALLVE 1
2 ALLGFCLNGLTIISFRKIKELRTPSNLLVLSIALADCGICINAFIAAFSSFLR 2
1 YWPYGSEGCQIHGFQGFLTALASISSSAAVAWDRYHHYCT 1
2 RSKLQWSTAISMMVFAWLFAAFWATMPLLGWGEYDYEPLRTCCTLDYSKGDR 2
1 NYITFLFALSIFNFMIPGFIMMTAYQSIHQKFKKSGHYK 0
0 FNTGLPLKTLVICWGPYCLLSFYAAIENVMFISPKYRM 0
0 IPAIIAKTVPTVDSFVYALGNENYRGGIWQFLTGQKIEKAEVDSKTK* 0

>RGR_xenTro
0 MVTSYPLPEGFTETEVFAIGTTLLVE 0
0 ALLGLLLNGLTLLSFYKIRELRTPSNLFIISLAVADTGLCLNAFVAAFSSFLR 2
1 YWPYGSEGCQIHGFQGFVAALSSIGSCAAIAWDRYHQYCT 1
2 RSKLHWSTAVSVVFFIWGFSAFWSAMPLFGWGEYDYEPLRTCCTLDYSKGDR 2
1 NYISYLFTMAFFEFLVPLFILMTAYQSIYQKMKKSGQIR 0
0 FNTSMPVKSLVFCWGPYCLLCFYAVIQDATILSPKLRM 0
0 IPALLAKTSPAVNAYVYGLGNENYRGGIWQYLTGQKLEKAETDNKTK* 0

>RGR_gasAcu stickleback DN680995
0 MVSSYPLPDGFTDFDVFSLGSCLLVE 0
0 GLLGILLNAVTIAAFLKVRELRTPSNFLVFSLAVADIGISMNATIAAFSSFLR 2
1 YWPYGSDGCQTHGFQGFVTALASIHFIAAIAWDRYHQYCT 1
2 RTKLQWSSAITLAVFVWLFTAFWSAMPLIGWGEYDYEPLRTCCTLDYTKGDR 2
1 NYVSYLIPMAIFNMAIQVFVVMSSYQSIAQKFKKTGNPR 0
0 FNPNTPLKAMLFCWGPYGILAFYAAVENATLVSTKLRM 0
0 MAPILAKTSPTFNVFLYALGNENYRGGIWQLLTGEKIDVPQIENKSK* 0

>PER_homSap peropsin RRH 4q25 7 chr4:110,968,599-110,985,310 337 + 16712 retinal photoisomerase Retinal pigment epithelium 
0 MLRNNLGNSSDSKNEDGSVFSQTEHNIVATYLIMA 1
2 GMISIISNIIVLGIFIKYKELRTPTNAIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYAGCQ 0
0 VYAGLNIFFGMASIGLLTVVAVDRYLTICLPDV 1
2 GRRMTTNTYIGLILGAWINGLFWALMPIIGWASYAPDPTGATCTINWRKNDR 2
1 SFVSYTMTVIAINFIVPLTVMFYCYYHVTLSIKHHTTSDCTESLNRDWSDQIDVTK 0
0 MSVIMICMFLVAWSPYSIVCLWASFGDPKKIPPPMAIIAPLFAKSSTFYNPCIYVVANKK 2
1 FRRAMLAMFKCQTHQTMPVTSILPMDVSQNPLASGRI* 0

>PER_monDom Monodelphis domestica (opossum)
0 MFKNNSVKTLAPEKEGPSVFSPIEHKIVAAYLITA 1
2 GVISIVSNVIVLGIFVKYKALRTATNTIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYDGCQ 0
0 IYAGLNIFFGMASIGLLTAVAIDRYLTICQPDL 1
2 GRMTSYNYTLMILTAWVNGFFWALMPIVGWAGYAPDPTGATCTINWRKNDV 2
1 SFVSYTMTVITINFAMPLGVMFYCYYNVSQKMKQYSPSNCPDHINRDWSNQVAVTK 0
0 MSVVMILMFLLAWSPYSIVCLWASFGDPKEIPPAMAIVAPLFAKSSTFYNPCIYVAANKK 2
1 FRRAISAMIRCQTHQSMPISNALPMN* 0

>PER_galGal Gallus gallus (chicken) 
0 MHWNDSANSSESDAEAHSVFTQTEHNIVAAYLITA 1
2 GVISIFSNIVVLGIFVKYKEFRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYTGCQ 0
0 IYAALNIFFGMASIGLLTVVAVDRYLTICRPDI 1
2 GRRMTTRNYAALILAAWINAVFWASMPTVGWAGYASDPTGATCTANWRKNDV 2
1 PFVSYTMSVIAVNFVVPLTVMFYCYYNVSRTMKQYTSSNCLESINMDWSDQVDVTK 0
0 MSVVMIVMFLVAWSPYSIVCLWSSFGDPKKISPAMAIIAPLFAKSSTFYNPCIYVIANKK 2
1 FRRAILAMVRCQTRQEITISNALPMTVSLSALTS* 0

>PER_xenTro Xenopus tropicalis (frog)
0 METLAEVSTLLPAGTGTVNISDASSEVHSVFSQSEHNIVAAYLITA 1
2 GVISILSNIIVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYVGCQ 0
0 IYAGLNIFFGMASIGLLTVVAIDRYLTICRPDIG 1
2 GRRISGRHYTAMILAAWINAVFWSVMPVVGWSSYAPDPTGATCTINWRKNDV 2
1 SFVSYTMSVVAVNFVVPLMVMFYCYYNVSRTMKGYGSRSSLGGINADWSDQTDVTK 0
0 MSMVMIVMFLVAWSPYSIVCLWSSFGDPRKIPPAMAIIAPLFAKSSTFYNPCIYVIANKK 2
1 FRRAILSMVQCKSRQEVTLDNHFPMNVSQSTLTT* 0

>PER_gasAcu Gasterosteus aculeatus (stickleback)
0 MGIDPEVNVTDDVTLYGGKSAFTQLEHNIVAGYLITA 1
2 GVISLFSNIVVLLMFWKFKELRTATNFIIINLAFTDIGVAGIGYPMSAASDIHGSWKFGYAGCQ 0
0 IYAALNIFFGMASIGLLTVVAIDRYLTICRPDIG 1
2 GQKMTMQSYNLLILAAWLNAVFWSSMPVVGWASYAPDPTGATCTINWRQNDV 2
1 SFISYTMAVIAVNFVLPLSAMFYCYYNVSATVKRYKASNCLDSANIDWSDQMDVTK 0
0 MSIVMIIMFLVAWSPYSIVCLWASFGDPKTIPAPMAIIAPLFAKSSTFYNPCIYVIANKK 2
1 FRRAIIGMVRCQTRQRITINSQVPMTTSQQPLTQ* 0

>PER_braBel Branchiostoma belcheri Amphiop3 peropsin AB050610
0 MDIPTETPYGAEEDIGESAGWRWTETDKNGFHKYDHLIVGLYLFVI
0 GIIGTIENGITLATFSKFRSLRSPTTMLLVHLAIADLGICIFGYPFSGASSLRSHWLFGGVGCQ 0
0 WYGFNGMFFGMANIGLLTCVAVDRYLVICRHDLV
2 DKVNYNTYGVMAALGWLFAAFWAALPLVGWAEYALEPSGTACTINFQKNDS 2
1 LYISYVTSCFVLGFVVPLAVMAFCYWQASCFVSKVLKGDIAGDLTFPVAANVDWEYQ
0 NHFSKMCLAMVAAFVVAWTPYSVLFLFAAFWNPADIPAWLTLLPPLIAKSSALYNPIIYIIANRR 0
0 FRNAICSMMKGQDPDVEDDEHADEHRVRSIEDNDKEIISMVNLNMTV* 0

>PERb_braBel Branchiostoma belcheri Amphiop2 peropsin RRH AB050607
MIPTNNNTENNDLEWGLEKEHGVSATIMGVYLTIVGLVATVGNATVVLMFIMKWRQLCRKAPNLLVINLAAANLCITIFG
YPFSASSGYAHQWLFPDAICTLYGFSCFLLSMVSMHTLCLISAHRYITICRPEHASKLTMNRTVLAVIGTWLYAIAVAVP
PLFNIARYTYEPSGLSCTIDFRVTTVADLVYLGSLIVLCYVIHVAVMATCYFKIIRKFSRHRFRQVRDIRTSHQRSFEMG
VTMRCILMTLFYLLSWTPYTAVCIWTMVGPPPPVVVSMAAALIAKTHCAFNPILYAFMSEVYRKLVFRTMCPCCFNRISC
KFVGTPTGGSKVSANPDIFTVDYNSRDQAVQINKAPSRRFCFVMETSEDLGSDDTGLTGHSGLWRSGAEVEGLGGLQVTQ
SPSVSGSELSLSLLDFLPPKPSGRAVSAKLPSPPALNSERATCPESSQQPSDRPATGLRQYQKGDTTRSSVGDLILTEDD
VTNLPPASETWGRKKSENPLSYRQTTRRTFGRSRKHSYIVD*

>PERc_braBel Branchiostoma belcheri Amphiop1 peropsin RRH AB050606 Go 
MNASPSSWLSSGEFFTDSPENSSEWPWTDGPTDTTWRHHQSVDSVSYEGYLASAIYITLTGLIAFFGNVITITVFLTEKE
FRKKQQNGFVLNLAIADLSVCVFAYPSSAIAGYAGRWVLGDVGCTIYGFLCFTFALVSMVTLCVISIYRYILICKPQYAH
LLTHRRTVYVIIGTWLYALVFTVPPLVGVKRYTYEPMQITCSLDWNVQHPGEKAYIAAVLVIVYVLQVLIMCFCYFNIIF
KSANLKFAALASEKTKMAAKKDTWKTSVMCLTMVVSFLIAWTPYAVSSTWDILSAEDLPIIATILPSLFAKSSCMMNPII
YACCNTK FRQAAVKSFRKLCGMCKQKVPLSTPQVVLAMQRNTEFTSTVEPTGQAFPMRVLPSISATHTAL*

>NEUR_homSap neuropsin 6p12.3 chr6:47,857,757-47,902,075 7 354
0 MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTII 1
2 GILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGIS 1
2 VVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSY 1
2 GVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTK 0
0 VAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYK FACCQTGGLKATKKKSLEGFR 2
1 LHTVTTVRKSSAVLEIHEE 0
0 WE* 0

>MEL1_homSap melanopsin rhabdomeric OPN4 10q23.2 + 11903 10 chr10 GLUD1 MMRN2 BMPR1A LDB3 OPN4 WAPAL GRID1
0 MNPPSGPRVPPSPTQEPSCMATPAPPSWWDSSQSSISSLGRLPSISPT 0 
0 APGTWAAAWVPLPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCR 2
1 SRSLRTPANMFIINLAVSDFLMSFTQAPVFFTSSLYKQWLFGET 1
2 GCEFYAFCGALFGISSMITLTAIALDRYLVITRPLATFGVASKRRAAFVLLGVWLYALAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMSFTPAVRAYTMLLCCFVFFLPLLIIIYCYIFIFRAIRETGR 2
1 ALQTFGACKGNGESLWQRQRLQSECKMAKIMLLVILLFVLSWAPYSAVALVAFAG 2
1 YAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPKYR 2
1 VAIAQHLPCLGVLLGVSRRHSRPYPSYRSTHRSTLTSHTSNLSWISIRRRQESLGSESEV 0
0 GWTHMEAAAVWGAAQQANGRSLYGQGLEDLEAKAPPRPQGHEAETPGK 0
0 TKGLIPSQDPRM* 0

>MEL1_monDom Monodelphis domestica (opossum) BMPR1A LDB3 OPN4 WAPAL GRID1
0 MNPSPMLRGLSCPAQDTNCTKIMASMSEWNNTEEDAYHLVDLPSIAPT 0
0 AVVLPPSSQNIFPTADVPDHAHYTIGATILAVGFTGVLGNLLVIYTFCR 2
1 LRTPANMFIINLAISDFFMSFTQAPVFFASSMYKRWIFGEK 1
2 ACEFYAFCGALFGITSMITLMAIALDRYFVITRPLASIGVISKKKTGFILLGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYTTFTPSVRAYTMLLFCFVFFIPLIVIIYCYIFIFRAIQDTNK 2
1 AVHSIGSGESTASPRHCQRMKNEWKMAKIALVVILLYVLSWAPYSTVALVAFAG 2
1 YSHILTPYMNSVPAIIAKASAIHNPIIYAISHPKYR 2
1 MAIAQNFPCLRALLCVRHPRTRSFSSYRFTRRSTMTSQASDISWLPRGRRQLSLGSESEI 0
0 GWNNMEAGTTSLTSRNQQGSCRMDQETMETRELAAIAKAKGRSWETLEK 0
0 TLEEMDDSSLLEVSVDMEQ* 0

>MEL1_galGal OPN4 melanopsin1 BMPR1A LDB3 OPN4 WAPAL GRID1
0 MDLPPRAPT 0
0 KMTVKDVRGAFPTVDVPDHAHYTIGTVILIVGITGTLGNFLVIYAFCR 2
1 SRTLQKPANIFIINLAVSDFLMSITQSPVFFTNSLHKRWIFGEK 1
2 GCELYAFCGALFGITSMITLMVIALDRYFVITKPLASVRVMSKKKALIILVGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCSWDYMTFTPSVRAYTMLLFCFVFFIPLIAIIYSYVFIFEAIKKANK 2
1 SVQTFGCKHGNRELQKQYHRMKNEWKLAKIALIVILLYVISWSPYSVVALVAFAG 2
1 YSHVLTPFMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 TAIATYVPCLGFLLRVSPKESRSFSSYPSSRRTTITSQSSETSGLQKGKRRLSSISDSES 0
0 GCTDTETDITSMISRPASSQVSYEMGEDTTQTSDLGGKPKVKSHDSGIFRK 0
0 TVVDADEIPMVEINDTEHSATSTCKTSEKCNVEEIQ 0
0 RSESLSGIGLREGESRHRTSASQIPSIIITYSNVQGVELHSGYSAGFLHPKNKSHKQNKSSNS* 0

>MEL1_xenTro Xenopus tropicalis melanopsin  
0 MNYQSVRKGITCPPQDANCSRILESLNSWNNSEVNSYKLVELPPIVTT 0
0 ETPQYEIHHVYPTVDVPDHVHYVVGAVILAVGITGMLGNFLVIYAFCR 2
1 SRSLRSPANMFIINLAITDFLMSVTQAPVFFATSLHKRWIFGEK 1
2 GCELYAFCGALFGITSMITLMVIAVDRYFVITRPLTSIGVMSKKRAVLILSGVWLYSLAWSLPPFFGW 1
2 SAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFCFVFFIPLFIIIYCYIFIFKAIKNTNR 2
1 AVQKIGTDNNKESHKQYQKMKNEWKMAKIALIVILLYVVSWSPYSTVALLAFAG 2
1 YASILTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAKYIPCLGSLLRVKRRDSRSYSSYPSSRRSTVTSHCSQSSDVGGHPKLKNHLPSVSDSES 0
0 GWTDTEADSSVNSRPASRQVSYEMGKDTTETNDLKSKAKLKSHDSGIFEK 0
0 TSMDADDISLVELGTVDRSSPIM 0
0 ANKHLNGLGQRKGDSFTRRSPSSRIPSIVVTHSNHQGSPAAVRHNSTLPGIKVSNSQDREKELKRQIEKVKQYVPIVTITSDTENSTGGFSNELLPANTS* 0

>MEL1_danRer Danio rerio (zebrafish) Gq XM_695228 +BMPR1A +LDB3 OPN4 +USP54 +CAMK2G  11 exons  AY882945 opn4d
0 MMSGAAHSVRKGISCPTQDPNCTRIVESLSAWNDSVMSAYRLVDLPPTTTTTTSVA 0
0 MVEESVYPFPTVDVPDHAHYTIGAVILTVGITGMLGNFLVIYAFSR 2
1 SRTLRTPANLFIINLAITDFLMCATQAPIFFTTSMHKRWIFGEK 1
2 GCELYAFCGALFGICSMITLMVIAVDRYFVITRPLASIGVLSQKRALLILLVAWVYSLGWSLPPFFGW 1
2 SAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFIFVFFIPLIVIIYCYFFIFRSIRTTNE 2
1 AVGKINGDNKRDSMKRFQRLKNEWKMAKIALIVILMYVISWSPYSTVALTAFAG 2
1 YSDFLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 LAIAKYIPCLRLLLCVPKRDLHSFHSSLMSTRRSTVTSQSSDMSGRFRRTSTGKSRLSSASDSES 0
0 GWTDTEADLSSMSSRPASRQVSCDISKDTAEMPDFKPCNSSSFKSKLKSHDSGIFEK 0
0 SSSDVDDVSVAGIIQPDRTLTN 0
0 AGDITDVPISRGAIGRIPSIVITSESSSLLPSVRPTYRISRSNVSTVGTNPARRDSRGGVQQGAAHLSNAAETPESGHIDNHRPQYL* 0

>MEL1d_danRer Danio rerio (zebrafish) frag Gq XM_695228 +BMPR1A +LDB3 OPN4 +USP54 +CAMK2G diff chrom, WGDup
0 QVAMVQDVRHPFPTVDVPDHAHYTIGSVILAVGITGMVGNLLVMYAFCK 2
1 SRSLRTPANMFIINLAVTDFLMCVTQTPIFFTTSLHKRWIFGEK 1
2 GCELYAFCGALFGICSMITLMIIAVDRYFVITRPLASIGVMSRKRALLILSAAWAYSMGWSLPPFFGW 1
2 SGAYVPEGLLTSCSWDYMTFSPSVRAYTMLLFTFVFFIPLFVIIYCYFFIFKAIRETNR 2
1 AVGKINGEGGPRDSIKKIHRMKNEWKMAKIALIVILLYVISWSPYSCVALTAF 2
1 YADMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 SAIAKYIPCLGVLLCVPRRDRFSSSSFISTRRSTLTSQSSETSSNLHRAGKARLSSVSDSES 0
0 GWTDTEADLSTASSRPASRQVSSEIRKDLCDIKHSSSLRLKVKSRDSGIFDR 0
0 0
0 QNDVSEKADEKRPLVRIPSIIVTSETCPAVLPAGHSSRLIPGAPAVTDS* 0

>MEL1_takRub Takifugu rubripes (teleost)
0 MNFGKSALQPPAQQSVVSCGGGGPEPNCTLRLAVTVMMSVRLAELQLHAST 0
0 LQVAMVRPFPTVDVPDHAHYTIGSVILVIGITGMIGNFLVIYAFCR 2
1 SRSLRTPANMFIINLAVTDLLMCVTQTPIFFTTSMYKRWIFGEK 1
2 GCELYAFCGALFGICSMITLTVIAIDRYFVITRPLTSIGVLSRKRAFVILMTVWIYSLGWSLPPFFGW 1
2 SGAYVPEGLLTSCTWDYMTFSPSVRAYTMLLFIFVFFLPLFIIIYCYFFIFRAIRATNK 2
1 AVGKVNGSVHSHSRRRESVKNFQRLQNEWKMAKIALMVILLYVISWSPYSCVALTAFAG 2
1 YADMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 LALAKYIPCLGFLLCISPHELQSTSSSFMSLRRSTVTSQTSDISGQFRPQSKPRRSSASDSES 0
0 CLTDTEADLSSMGSRPASRQVSCDISRDTTELPEYKPASSFNSKVKSPDSGIFEK 0
0 TSFDFDASMAASRERSSIPN 0
0 SGEFPEGHVMRRTLARIPSIIITSESSHFLPNGRKASSTTCIANGSDIKVGPR* 0

>MEL1_gasAcu Gasterosteus aculeatus (stickleback)
0 MNAGESELLLPTQQSILPCGDHEPNCPVAQAETLALSAASANGSA 0
0 VQVAMVSRAPHPYPTVDVPDHAHYTIGSVILAIGITGIIGNVLVIYAFSK 2
1 SRSLRTPANMFIINLAITDLLMCVTQAPIFFTTSMHKRWIFGEK 1
2 GCELYAFCGALFGICSMITLTVIALDRYFVITRPLTSIGMMSRRRALLILMGAWTYSLGWSLPPFFGW 1
2 SGAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFIFVFFLPLFIIIYCYFFIFRAIRVTNR 2
1 AVGKMNGSIHSHGSGRDSTKNFHRLQNEWKMAKIALIVILLYVVSWSPYSAVALTAFAG 2
1 YADMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 IALAKYIPFLGVLLCVPPRELRSASSSFRSTRRSTVTSQTSDVSSQQRRQGSRNSRLSSASDSES 0
0 CLTDTEADGSSVGSRPASRQVSCDIGRDTAELPEFKPSSSFKSKMKSHDSGIFEK 0
0 SYDTDISMAGVSERGSIPN 0
0 QTDFAEGRDRRSTIGRIPSIVITSETSPFLPTGRNGSCNGRPKTANSSHPGAGSG* 0

>MEL1_oryLat Oryzias latipes (medaka) frag
0 LQVAMVPQTFHPFPTVDVPDHAHYTIGSVILAIGITGIIGNFLVIYAFSR 2
1 SRSLRTPANMFIINLAITDLLMCVTQSPIFFTTSMHKRWIFGEK 1
2 GCELYAFCGALFGICSMITLTVIAIDRYFVITRPLTSIGVLSRKRALLILSAAWAYSLGWSLPPFFGW 1
2 SGAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFIFVFFLPLFIIIYCYVFIFRAIRSTNR 2
1 AVGKINGNTRDAVKSFNRLQNEWKMAKIALIVILLYVISWSPYSTVALTAFAG 2
1 YADMLTPYMNSIPAVIAKASAIHNPIIYAITHPKYR 2
1 MALAKYIPGLGVLLCIHPKDLRSASSSFVSTRRSTVTSQSSDISSQLRRQSTFKSRLSSLSDSES 0
0 GLTDTEADLSSLSSRPASRQVSCEISRDTAELPDFKHTSSFKAKLKNNDSGIFEK 0
0 TSFDTVSIGGVSEHNSIPS 0
0 NRDFGDGNVTRATIGRIPSIVVTSEMSPFLPVGRNGSRTNRSKMANSSAGAGPV* 0

>MEL1_calMil Callorhinchus milii (elephantfish) frag
0 ASVTDAQHHHMFPTVDVPDHAHYIIGATILAVGVTGMVGNFLVIYAFLRYH 2
1 SRSLRTPANTFIINLAATDFLMSVTQSPIFFITSIHKRWIFGEK 1
2 GCELYAFCGALFGITSMITLMVIALDRYFVITRPLASIGVLSHRRAGLIILSLWLYSLAWSLPPFFGW 1
2 SGAYVPEGLLTSCTWDYMTFTPSVRAYTMLLFCFVFFIPLGVIIYCYIFIFRAIKSTNK 2
1 KVGGSTNRESQKQHQRMKNEWKMAKIALIVILLFVISWSPYSTVALTAFAG 2
1 YADMLTPYMNSVPAVIAKASAIHNPIIYAITHPKYR 2
1 MAIAKYVPLLGLLLRVSRRDSRTSGQYYSTRRSTLTSQTSDLSGYPRGKGRLSSASDSES 0

>MEL1a_braFlo Branchiostom floridae AmphiMop 12 exons 709aa +chrUn:47589027-47597447 size 8421 +tandem dup assembly error
0 MTELPSFQPPTNSTEEENAVFPTALTEWISE 0
0 VGNQVGEAALKLLSGEGDGMEVTPTPGCTGNASVCNGTDSGGGVVWDIPPLAHYIVGTAVFCVGCCGMFGNAVVVYSFIK 2
1 SKGLRTPANFFIINLALSDFLMNLTNMPIFAVNSAFQRWLLSDF 1
2 ACELYGFAGGLFGCLSINTLMAISMDRYLVITKPFLVMRIVTKQR 0
0 VMFAILLLWIWSLVWALPPLFGWSAYVPEGF 1
2 GTSCTFDYMTPKLSYHIFTYIIFFTMYFIPMGVIIYCYYNIFATVKSGDKQFGKAVKEMAHEDVKNK 0
0 AQQERQRKNEIKTAKIAFIVITLFLSAWTPYAVVSALGTLGYQDLVTPYLQSIPAVFAKSSAVYNPI 1
2 VYAITHPKFRAAVKKHIPCLSGCLPADEEETKTKTRGATTTASMSMTQTTAPTV 0
0 HDPQASVHSGSSVSVDDSSGVSRQDTMMVK 0
0 VEVDNRMEKAGGGAADTAPKDGTSVPTVSAQIEVRPSGNVNTKAEVIPSPQSAAVAHGASASPVPK 0
0 VAELSSSVSLESAAIPGKIPTPLPSQPIAAPIERHMAAMADDPPPKPRGVATTVNVRRSESGYERSQDSLRKK 0
0 AVSETRSRSFNSTKDHFASERQTSTTLNQPRDMYSGDMVKKTRQSPEKQEYDNPAFDAGIAEIDTDSENETEGSYDMLSVRFQAMAEEPPVETYRKASDMSINLGKASLMLTEAHDETVL* 0

>MEL1a_braBel Branchiostoma belcheri (Japanese lancelet) AB205400 Amphi-mop
0 MTEIPSFQPPINATEVEEENAVFPTALTEWFSE 0
0 VGNQVGEVALKLLSGEGDGMEVTPTPGCTGNGSVCNGTDSGGVVWDIPPLAHYIVGTAVFCIGCCGMFGNAVVVYSFIK 2
1 SKGLRTPANFFIINLALSDFLMNLTNMPIFAVNSAFQRWLLSDF 1
2 ACELYGFAGGLFGCLSINTLMAISMDRYLVITKPFLVMRIVTKQR 0
0 VMFAILLLWIWSLVWALPPLFGWSAYVSEGF 1
2 GTSCTFDYMTPKLSYHIFTYIIFFTMYFIPGGVMIYCYYNIFATVKSGDKQFGKAVKEMAHEDVKNK 0
0 AQQERQRKNEIKTAKIAFIVISLFMSAWTPYAVVSALGTLGYQDLVTPYLQSIPAMFAKSSAVYSPI 1
2 VYAITYPKFREAVKKHIPCLSGCLPASEEETKTKTRGQSSASASMSMTQTTAPV 0
0 HDPQASVDSGSSVSVDDSSGVSRQDTMMVK 0
0 VEVDKRMEKAGGGAADAAPQEGASVSTVSAQIEVRPSGKVTTKADVISTPQTAHGLSASPVPK 0
0 VAELGSSATLESAAIPGKIPTPLPSQPIAAPIERHMAAMADEPPPKPRGVATTVNVRRTESGYDRSQDSQRKK 0
0 VVGDTHRSRSFNTTKDHFASEQPAALIQPKELYSDDTTKKMARQSSEKHEYDNPAFDEGITEVDTDSENETEGSYDMLSVRFQAMAEEPPVETYRKASDLAINLGKASLMLSEAHDETVL* 0

>MEL1b_braBel Branchiostoma floridae Amphiop6 melanopsin approx Gq
0 MSPNLTNTSLLPNRTDRPELSPADVTMQLVFGSMMLVFGLIGVVGNAVALYAFCR 2
1 SRSLRRPKNYLIANLCLTDMVVCLVYSPIIVTRSLSHG 2
1 LPSKESCIVEGFVVGLGSIVSICSLAGIAVERYVTITQPIKSLSILTHRALLGAVSAVWVYAFLLAFPPLVGWGRYVSEESKISCTFDYLSTDDATRAHVIVLVIGAFGLPFS
VITYCYVRSFATVRKCTKERKQMSPLAKSDSRSEVKAAVNSFVITTSFCLCWCPYAVVATMGVSGFTVHSHAVFIAALLAKLSVLFNPVAYVLSIP 1
2 NSNVNIESTELTVPYSASRESCLLSRAATERLAGRSPSLTDIVREFGLQQTASHRE

>MEL1b_braBel Branchiostoma belcheri Amphiop6 melanopsin AB050611  Gq
0 MSSNLTNVSLVANRTDQTELSPTDVTMQLIFGSMMLVFGLIGVVGNVVALYAFCR 2
1 TRSLRRPKNYVVANLCLTDMFVCLVYCPIVVSRSFSHG 2
1 FPSKESCIVEGFMVGVGSIASICSLAAIAVERYLSVTQPLKSLTILTQRKLLVAVLTVWVYSLLLAFPPLVGWGRYVREETYISCTFDYLSTDDATRAYVITLVMGAFGFPLL
TIAYCYIRVFTTARKHAEERKFMSPLKRPESRTEIKTAVTACVITTSFCLCWCPYAVVATLGISGVSVQQQTVFSAALLAKLTVIINPIVYVLSIPNFRKALFAQEREKYASED
VVLTSLPGKTRRMKKVERSQSSNSNVVIEVKESSMAYSTSRESCLLSRAATKRLAGKTKSIVDLVDEFGLQETAPHKESLV*

>MEL2_galGal melanopsin2 GRID2 SMARCAD1 PGDS MEL2 SEC24B COL25A1
0 MGTQPHSVTKSEIPDHVLYTVGTCVLVIGSIGIIGNLLVLYAFYS 2
1 NKKLRTPQNFFIMNLAVSDFLMSASQAPICFVNSLHREWILGDI 1
2 GCDLYAFCGALFGITSMMTLLAISVDRYLVITKPLRSIQWTSKKRTIQIIAAVWLYSLGW 1
2 SVAPLLGWSSYVPEGLMISCTWDYVTYSPANRSYTMILCCCVFFIPLIIILHCYLFMFLAIRSTGR 2
1 DVQKLGSCSRKSFLSQSMKNEWKLAKIAFVVIIVYVLSWSPYACVTLIAWAG 2
1 RGNTLTPYSKSVPAVIAKASAIYNPIIYAIIHPRYRK 2
1 TIHNAVPCLRFLIRISKNDLLRGSINESSFRTSLSSHQSLAGRTKNTCVSSVSTGEA 0
0 NWSDVELDTVEPAHEKLQPRRSHSFSSSLRQKRDLLPDSYSCSEETEEK 0
0 VSLSSSYLEKVLGRSAFPSSPVALVTSSLRAASLPVGLNSSSASRGAGSDISQMKTEESHNNGGLDSIVSNTVPQIIIIPTSETNLFQEEPEEEETELFHFHDKKNNLLDLEGLSSSTEFLEAVEKFLS* 0

>MEL2_anoCar melanopsin2 frag GRID2 SMARCAD1 MEL2 PDILM5 BMPR1B UNC5B
0 MGPHHRTKVDVPDHVLYTVGSCVLVIGCIGITGNLLVLYAFYS 2
1 NKRLRTPPNYFIMNLAVSDFLMSATQAPICFLNSMHKEWVLGDI 1
2 GCNLYAFCGALFGITSMITLLAISVDRYCVITKPLQSIKRTSKKRTCIIIVFVWLYSLGWSVCPLFGW 1
2 SSYIPEGLMISCTWDYVTYSPANRSYTMMLCCCVFFIPLVIIFHCYIFMFLAIRSTGR 2
1 RKSSISHSIKSEWKLAKIAFVAIVVFVLSWSPYACVTLISWAG 2
1 TLTPYSKSVPAVIAKASAIYNPIIYAIIHPRYRK 2
1 TIRSAVPCLRFLIPISKSDLSTSSMSESSFRASVSSRHSFSYRNKSTYISSISAKET 0
0 TWCDVELDPVESGHKKLQAYRSNSFSAKGVAEEESGLLLRTNNCNVPARKK 0

>MEL2_xenLae Xenopus laevis melanopsin (Xmop) SMARCAD1 
0 MDLGKTVEYGTHRQDAIAQIDVPDQVLYTIGSFILIIGSVGIIGNMLVLYAFYR 2
1 NKKLRTAPNYFIINLAISDFLMSATQAPVCFLSSLHREWILGDI 1
2 GCNVYAFCGALFGITSMMTLLAISINRYIVITKPLQSIQWSSKKRTSQIIVLVWMYSLMWSLAPLLGW 1
2 SSYVPEGLRISCTWDYVTSTMSNRSYTMMLCCCVFFIPLIVISHCYLFMFLAIRSTGR 2
1 NVQKLGSYGRQSFLSQSMKNEWKMAKIAFVIIIVFVLSWSPYACVTLIAWAG 2
1 HGKSLTPYSKTVPAVIAKASAIYNPIIYGIIHPKYRE 2
1 TIHKTVPCLRFLIREPKKDIFESSVRGSIYGRQSASRKKNSFISTVSTAET 0
0 VSSHIWDNTPNGHWDRKSLSQTMSNLCSPLLQDPNSSHTLEQTLTWPDDPSPKEILLPSSLKSVTYPIGLESIVKDEHTNNSCVR
NHRVDKSGGLDWIINATLPRIVIIPTSESNISETKEEHDNNSEEKSKRTEEEEDFFNFHVDTSLLNLEGLNSSTDLYEVVERFLS* 0

>MEL2_danRer Danio rerio (zebrafish) FLJ39155 PDILM5
0 MEPQRQIYKRLDVPDHVHYIIAFLILIIGTLGVSGNALVMFAFYR 2
1 NKKLRSLPNYFIMNLAVSDFLMAITQSPIFFINCLYKEWMFGEL 1
2 GCKIYAFCGALFGITSMINLLAISIDRYLVITKPLQTIQWNSKRRTGLAILCIWLYSLAWSLAPLIGW 1
2 GSYIPEGLMTSCTWDYVSPSPANKSYTMMLCCFVFFIPLSIILYCYLFMFLSVRQASR 2
1 QKSSFVKQQSMRSEWKLAKIAAVVIVVYVLSWAPYACVTLVAWAG 2
1 LTPYSKTLPAVLAKSSAIYNPFIYAIIHNKYRA 2
1 TLAEKVPGLSCLSRSQKDGLSSSTNSDASAQDSSVSRQSSVSKNRLHSTMVQ* 0

>MEL2_tetNig frag +BMPR1B
0 MEPKDTHITSSFFSKVDVPDHVHYIIAFFVFVIGILGITGNVLVIFAFYS 2
1 NKKLRSLPNYFIVNLAVSDLLMASTQSPIFFINLYKEWMFGET 1
2 ACKMYAFCGALFGITSMINLLAISVDRYVVITKPLQTIRRSSKRRTALAILMVWLYSLAWSLAPLVGW 1
2 GSYIPEGLMTSCTWDYVTYTLANRSYTMMLCCFVFFIPLAIILCCYLLMFLAIRKTSR 2
1 RKSTLIQQKSIRSEWKLAKIAFVVIVVYVLSWSPYACVTLISWAG 2
1 TLTPYSKSVPAVIAKASAIYNPIIYAIIHPRYRK 2
1 TIRSAVPCLRFLIPISKSDLSTSSMSDSSFRSALSCRHSYRSRSTYISSISAKET 0
0 TWCDVELDPVESGHKKLQAYRSNSFSAKGVAEEESGLLLRTNNCNVPARKK 0

>MEL2_gasAcu Gasterosteus aculeatus KNTC2 FLJ39155  PDLIM5 BMPR1B
0 MEPDNAHTQRSFINKVDVPDHAHYIVAVFVVVIGTLGITGNALVMLAVYS 2
1 NKKLRNLPNYFIMNLAVSDFLMAFTQSPIFFINCLYKEWAFGET 1
2 GCKIYAFCGALFGIASMINLLAISIDRYLVITKPLQAIHWGSKRRTTLAILLVWLYSLAWSLAPLVGW 1
2 GSYIPEGLMTSCTWDYVTYTLANRSYTMMLCCFVFFIPLGIILYCYLFMFLAIRKTSR 2
1 RKSTLIKQKSMKSEWKLAKIAFVVIVVYVLSWSPYACVTLISWAG 2
1 ILSPYSKAVPAIIAKASAIYNPFIYAIIHNKYRM 2
1 TLAAKFPCLRFLSPTPRKDTSSSISESSYRDSVISRQSTASRTHFITACPDTVN 0

>RHAB_plaDum Platynereis dumerilii (clam worm) rhabdomeric opsin Gq AJ316544 runs as melanopsin unavailable genomically
MSRSEVLVPGSMSLDGLLTTAHPIGNDSIETILHPYWQQFDIENTIPDSWHYAVAAWMTFFGILGVSGNLLVVWTFLKTKSLRTAPNMLLVNLAIGDMAF
SAINGFPLLTISSINKRWVWGKLWRELYAFVGGIFGLMSINTLAWIAIDRFYVITNPLGAAQTMTKKRAFIILTIIWANASLWALAPFFGWGAYIPEGFQ
TSCTYDYLTQDMNNYTYVLGMYLFGFIFPVAIIFFCYLGIVRAIFAHHAEMMATAKRMGANTGKADADKKSEIQIAKVAAMTIGTFMLSWTPYAVVGVFG
MIKPHSEMFIHPLLAEIPVMMAKASARYNPIIYALSHPKFRAEIDKHFPWLLCCCKPKPKAQLPSSTTKGSIASKTEADTSV*

>RHAB1_apiMel Apis mellifera long wavelength sensitive opsin 1 (Lop1) NM_001077825 compound eyes
MIAVSGPSYEAFSYGGQARFNNQTVVDKVPPDMLHLIDANWYQY
PPLNPMWHGILGFVIGMLGFVSVMGNGMVVYIFLSTKSLRTPSNLFVINLAISDFLMM
FCMSPPMVINCYYETWVLGPLFCQIYAMLGSLFGCGSIWTMTMIAFDRYNVIVKGLSG
KPLSINGALIRIIAIWLFSLGWTIAPMFGWNRYVPEGNMTACGTDYFNRGLLSASYLV
CYGIWVYFVPLFLIIYSYWFIIQAVAAHEKNMREQAKKMNVASLRSSENQNTSAECKL
AKVALMTISLWFMAWTPYLVINFSGIFNLVKISPLFTIWGSLFAKANAVYNPIVYGIS
HPKYRAALFAKFPSLACAAEPSSDAVSTTSGTTTVTDNEKSNA*

>RHAB2_apiMel Apis mellifera long wavelength sensitive opsin 2 (Lop2) NM_001077825 ocelli
MDTLNITTSFFIEVMPSNISTLTTTGPQFARQLMRFNNQTVVSK
VPEEMLHLIDLYWYQFPPLDPLWHKILGLVMIILGIMGWCGNGVVVYVFIMTPSLRTP
SNLLVVNLAFSDFIMMGFMCPPMVICCFYETWVLGSLMCDIYAMVGSLCGCASIWTMT
AIALDRYNVIVKGMSGTPLTIKRAMLQILGIWLFGLIWTILPLVGWNRYVPEGNMTAC
GTDYLSQDWTFKSYILVYSFFVYYTPLFTIIYSYYFIVSAVAAHEKAMKEQAKKMNVT
SLRSGDNQNTSAEAKLAKVALTTISLWFMAWTPYLVINYIGIFNRSLITPLFTIWGSL
FAKANAIYNPIVYGISHPKYRAALKEKLPFLVCGSTEDQTAATAGDKASEN*

>RHAB3_apiMel Apis mellifera UV-sensitive opsin BK005513
MSNDSIHWEARYLPAGPPRLLGWNVPAEELIHIPEHWLVYPEPN
PSLHYLLALLYILFTFLALLGNGLVIWIFCAAKSLRTPSNMFVVNLAICDFFMMIKTP
IFIYNSFNTGFALGNLGCQIFAVIGSLTGIGAAITNAAIAYDRYSTIARPLDGKLSRG
QVILFIVLIWTYTIPWALMPVMGVWGRFVPEGFLTSCSFDYLTDTNEIRIFVATIFTF
SYCIPMILIIYYYSQIVSHVVNHEKALREQAKKMNVDSLRSNANTSSQSAEIRIAKAA
ITICFLYVLSWTPYGVMSMIGAFGNKALLTPGVTMIPACTCKAVACLDPYVYAISHPK
YRLELQKRLPWLELQEKPISDSTSTTTETVNTPPASS*

>RHAB4_apiMel Apis mellifera blue-sensitive opsin (Blop)
MLLHNKTLAGKALAFIAEEGYVPSMREKFLGWNVPPEYSDLVHP
HWRAFPAPGKHFHIGLAIIYSMLLIMSLVGNCCVIWIFSTSKSLRTPSNMFIVSLAIF
DIIMAFEMPMLVISSFMERMIGWEIGCDVYSVFGSISGMGQAMTNAAIAFDRYRTISC
PIDGRLNSKQAAVIIAFTWFWVTPFTVLPLLKVWGRYTTEGFLTTCSFDFLTDDEDTK
VFVTCIFIWAYVIPLIFIILFYSRLLSSIRNHEKMLREQAKKMNVKSLVSNQDKERSA
EVRIAKVAFTIFFLFLLAWTPYATVALIGVYGNRELLTPVSTMLPAVFAKTVSCIDPW
IYAINHPRYRQELQKRCKWMGIHEPETTSDATSAQTEKIKTDE*

>RHO1_calMil Callorhinchus milii complete wgs cannot find cone opsins
0 MNGTEGENFYIPMSNKTGVVRSPFEYPQYYLAEPWQFSILAAYMFFLIITCFPVNFLTLYVTFEHKKLRQPLNFILLNLAVADLFMVFGGFFITVYTSLHGYFVFGVTGCNFEGFFATLG 1
2 GEIGLWSLVVLAIERYVVVCKPMSNFRFGTNHAIMGVAFTWVMALACAVPPLMGWSR 2
1 YIPEGLQCSCGVDYYTLKPEINNESFVIYMFVVHFLIPLIIIFFCYGRLVCTVKE 0
0 AAAQQQESESTQRAEREVTRMVIIMVIFFLICWVPYASVAFFIFTNQGSEFGPIFMAVPAFFAKSSALYNPLIYILLNKQ 0
0 FRNCMITTLCCGKNPFEEDESTSAAASKTEASSVSSSQVSPA* 0

>LWS_calMil Callorhinchus milii can just barely see novel exon break
01
2 dPFEGPNYHIAPRWAYNLTSVWMVGVVVASVFTNGLVLVATVRFKKLRHPLNWILVNMALADLGETVLASTVSVANQFFGYFILGHPLCVFEGFVVSLC 1
2 GITALWSLTIIAWERWVVVCKPFGNVKFDGKWAAFGIIFSWVWSIGWCLPPVFGWSR 2
1 0
0 AEKEVSRMVVVMVAAFCLCWGPYACFAMFSALNPGYAFHPLVASIPSYFAKSSTIYNPIIYVFMNRQ 0
0 FRNCILQLFGKKVDDGSELSSTSKTDVSSVSNSSVSPA* 0

>PIN_calMil Callorhinchus milii
0      FGSTVSFSNNINGYFVLGETVCQFEGFMVSLT 1
2 GIVGLWSLAILAFERYIVICKPMGDFRFQQKHAVWGCLFTWLWSLFWTLPPLFGWCSYVPE 1

>PPIN_calMil
0 MDPHNRSANLSEGPGLGGGGAVPGWGPSVRAPLSLVMAVISLSSIVLNSLAIAVVLRFQVLQQPLNYALLSLASADLGTAATGGVLSTVCTALGSFVLGRHSCVAEGFF 1 

>VAOP_calMil Callorhinchus milii fragment
0 VASTQGRLGVARKPEKQVTRMVIVMILAFLFCWTPYAAFSITVTACPTIKLDPRLAAIPAFFSKTATVYNPIIYVFMNKQ 0

>RGR_calMil Callorhinchus milii  
0    EGFTDFEVFGLGTALLVE 0
0 GLVGLLLNGLTLLAFYKIKELRTPSNLLITSLALSDFGISMNAFIAAFSSFLR 2
1 YWPYGSEGCQTHGFHGFLMALASINACAAIAWDRYHQNCS 1
2 SRLQWSSAITVTVFIWGIAAFWSAMPLLGWGVYDYEPLRTCCTLDYSKGDR 2
1  EFIFPIFIMLSSYQSCKSKFKKTGQVK 0
0 FNTGLPVKTLIFCWGPYSLLCFYATIENITILSPKLRM 0

>PER_calMil Callorhinchus milii 
1 LFVSYTMTVIAVNFVVPLSVMFFCYYNVSKTMSRFISSPSPENINLDWSDQLDVTK 0
0 MSVVMIVMFLLAWSPYSIVCLWASFGNPKLIPPAMAIIAPLFAKSSTFYNPCIYVIANKK 2
1 FRKAIMAMICCQNRQEITINHTLPMTISRVPLTE* 0

>ENCEPH_calMil Callorhinchus milii 1099306574966 mainstream fragment
0 MNPTNSTEPQEEHLFSPNTYKLLAVIIGTIGIVGFCNNILVLLLYYKFKRLRTPTNLLLVNISVSDLLVSVFGLSFTFVSCTQGRWGWDSAACVWDG

>ENCEPH4_calMil Callorhinchus milii like branch4/5 only missing internal intron holdover
0 MLNSSPNSSPSLPLSQVGWTGLSRTGLTVVAVCLGIIMVLGFLNNLLVLVLFCKYKVLRSPMNMLLLNISVSDMLVCICGTPFSFAASVQGRWLVGEQGCKWYGFANSLF 1
0       REHRILLMVISMVTFYLLCWLPYGTVALIGTFGNADLITPTCSVIPSILAKSSTVINPVIYVIMNKQ 0

>ENCEPH5_calMil Callorhinchus milii 1099306574966 fragment
AQTREHRILLMVISMVTFYLLCWLPYGTVALIGTFGNADLITPTCSVIPSILAKSSTVINPVIYVIMNKQ 0

>MEL1b_calMil Callorhinchus milii fragment 70%
1 SKSLRTPANMFIINLAISDFFMSATQPPVFFVTSLHKRWIFGEK
2 GCKLYAFCGALFGITSMITLMAISIDRYWVITKPLQSISSTTTKKNTLKVIILVWLYSLAWSLPPLLGW 1

>ENCEPH_squAca Squalus acanthias (spiny dogfish) EB687868 fragment
0 MNAANSTDTREESLFSPGTYQVLAVIIGTIGVVGFCNNLLMLVLYCKFKRLRTPTNLFLVNISISDLLLSVFGVIFTFVSCVKGRWVWDSAACVWDGFSNCLF 1
2 GISSIMSLTVLAYERYIRVVNATAIDFSWAWRAITYIWLYSLAWTGAPLIGWNSYTLELHRLGCSVNWDSRNPSDTSFVLFLFLGCLLCPIGVIAYCYG

>ENCEPH_petMar Petromyzon marinus fragment
0 MQSPKQDSLHYAGDTGAKAAPDSAQGNASALGSNFLLHGGDLGEGSTAFSAATFRLLAGVVGTIGVAGFLNNLLLVALFVGFKRLQTPTNLLLVNISLSDLLVSVFGNTLTLVSCVRRRWVWGNGGCVWDGFSNSLF 1
2 GIVSISTLTALSYERYARLIKAQVLDFSWAWRAVTYTWLYSAAWTGAPLLGWSRYVLEKHGLGCSIDWASSNPPDAAFVLFFFLGCLAAPLLVMGFCFGRIALAITQ 0
0      CWSPYAVASLFVASGFEHLVSPPVSIVPSLLAKSNAVCNPLLFLLMSGN 0

>MEL1_petMar lamprey traces frag 80%
1 SKSLRSPANIFIINLAFADFFMSITQTPIFFVTSLHKRWIFGEK 1
2 GCELYAFCGALFGIASMVTLMVIATDRYLVLTRPLASIGAMSKRRAMYITAAVWFYSLAWSLPPFFGW 1
2 AYVPEGLMTSCTWDYVTFTPAVRSYTMLLFCFVFFIPLIVIIFCYVRIFAAIKNTNR 2
1 YADMLTPYMNSVPAIIAKASAIHNPIVYAITHPKYR 2

>PARIE_petMar
0 LNKKIKRVGGHPDPREEMRATVMVLAMVGAFLACWLPYTVLALCVVLAPGTQIPPLVATLPMYFAKTSPMYNPIIYFFLNPQ 0

Here are those sequences aligned to show rare genomic events such as indels and intron gains and losses:


>RHO1_homSap LAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWS
>RHO1_monDom LAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAIADLFMVFGGFTMTLYTSLHGYFVFGPTGCNLEGFFATLG GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIIGVAFTWVMALACAFPPLIGWS
>RHO1_ornAna LAAYMFMLIMLGFPINFLTLYVTIQHKKLRTPLNYILLNLAFANHFMVLGGFTTTLYTSLHGYFVFGPTGCNIEGFFATLG GEIALWSLVVLAIERYIVVCKPMSNFRFGENHAIMGVAFTWIMALACALPPLVGWS
>RHO1_galGal LAAYMFMLILLGFPVNFLTLYVTIQHKKLRTPLNYILLNLVVADLFMVFGGFTTTMYTSMNGYFVFGVTGCYIEGFFATLG GEIALWSLVVLAVERYVVVCKPMSNFRFGENHAIMGVAFSWIMAMACAAPPLFGWS
>RHO1_anoCar LAAYMFLLILLGFPINFLTLFVTIQHKKLRTPLNYILLNLAVANLFMVLMGFTTTMYTSMNGYFIFGTVGCNIEGFFATLG GEMGLWSLVVLAVERYVVICKPMSNFRFGETHALIGVSCTWIMALACAGPPLLGWS
>RHO1_xenTro LAAYMFLLILLGFPINFMTLYVTIQHKKLRTPLNYILLNLVFANHFMVLCGFTVTMYTSMHGYFIFGQTGCYIEGFFATLG GEMALWSLVVLAIERYVVVCKPMANFRFGENHAIMGVVFTWIMALSCAAPPLFGWS
>RHO1_danRer VAAYMFFLIITGFPVNFLTLYVTIEHKKLRTPLNYILLNLAIADLFMVFGGFTTTMYTSLHGYFVFGRLGCNLEGFFATLG GEMGLKSLVVLAIERWMVVCKPVSNFRFGENHAIMGVAFTWVMACSCAVPPLVGWS
>RHO1_Raja   LAAYMFFLILTGLPVNFLTLFVTIQHKKLRQPLNYILLNLAVSDLFMVFGGFTTTIITSMNGYFIFGPAGCNFEGFFATLG GEVGLWCLVVLAIERYMVVCKPMANFRFGSQHAIIGVVFTWIMALSCAGPPLVGWS
>RHO1_lamp1  LAAYMFFLILVGFPVNFLTLFVTVQHKKLRTPLNYILLNLAVANLFMVLFGFTLTMYSSMNGYFVFGPTMCNFEGFFATLG GEMSLWSLVVLAIERYIVICKPMGNFRFGSTHAYMGVAFTWFMALSCAAPPLVGWS
>RHO1_lamp2  LAAYMFFLILVGFPVNFLTLFVTVQHKKLRTPLNYILLNLAVSNLFMILFGFTTTMYTSMNGYFVFGPTMCSIEGFFATLG GEVSLWSLVVLAIERYIVICKPMGNFRFGNTHAIMGVALTWVMALSCAAPPLLGWS
>RHO1_lamp3  LAAYMFFLILVGFPVNFLTLFVTVQHKKLRTPLNYILLNLAMANLFMVLFGFTVTMYTSMNGYFVFGPTMCSIEGFFATLG GEVALWSLVVLAIERYIVICKPMGNFRFGNTHAIMGVAFTWIMALACAAPPLVGWS
>RHO2_galGal VCCYIFFLISTGLPINLLTLLVTFKHKKLRQPLNYILVNLAVADLFMACFGFTVTFYTAWNGYFVFGPVGCAVEGFFATLG GQVALWSLVVLAIERYIVVCKPMGNFRFSATHAMMGIAFTWVMAFSCAAPPLFGWS
>RHO2_anoCar VCCYIFFLIFTGLPINILTLLVTFKHKKLRQPLNYILVNLAVADLFMACFGFTVTFYTAWNGYFIFGPIGCAIEGFFATLG GQVALWSLVVLAIERYIVVCKPMGNFRFSATHALMGISFTWFMSFSCAAPPLLGWS
>RHO2_Gekko  LSFYMFFLIAAGMPLNGLTLFVTFQHKKLRQPLNYILVNLAAANLVTVCCGFTVTFYASWYAYFVFGPIGCAIEGFFATIG GQVALWSLVVLAIERYIVICKPMGNFRFSATHAIMGIAFTWFMALACAGPPLFGWS
>RHO2_Lat    LCAYMFLLIILGFPINFLTLLVTFKHKKLRQPLNYILVNLAVASLFMVVFGFTVTFYSSLNGYFVLGPMGCAMEGFFATLG GQVALWSLVVLAIERYIVVCKPMGNFRFASSHAIMGIAFTWIMALACAAPPLVGWS
>RHO2_Geo    ISAYVFTLILIGFPVNFMTLFVTFKLKKLRQPLNFILVNLCVADLLMIMFGFTTTFYTAMNGYFVFGPTGCNIEGFFATLG GEVSLWSLVMLAIERYIVVCKPMGNFRFATTHAALGVVFTWVMASACAVPPLVGWS
>SWS1_homSap QAAFMGTVFLIGFPLNAMVLVATLRYKKLRQPLNYILVNVSFGGFLLCIFSVFPVFVASCNGYFVFGRHVCALEGFLGTVA GLVTGWSLAFLAFERYIVICKPFGNFRFSSKHALTVVLATWTIGIGVSIPPFFGWS
>SWS1_macDom QTVFMGFVFCAGTPLNAVVLVATLRYKKLRQPLNYILVNVSLCGFIFCIFAVFTVFISSSQGYFIFGRHVCAMEAFLGSVA GLVTGWSLAFLAFERFIVICKPFGNFRFNSKHAMMVVLATWVIGIGVSIPPFFGWS
>SWS1_galGal QTAFMGIVFAVGTPLNAVVLWVTVRYKRLRQPLNYILVNISASGFVSCVLSVFVVFVASARGYFVFGKRVCELEAFVGTHG GLVTGWSLAFLAFERYIVICKPFGNFRFSSRHALLVVVATWLIGVGVGLPPFFGWS
>SWS1_Taenio QTIFMGLVFVAGTPLNAIVLIVTIKYKKLRQPLNYILVNISVSGLMCCVFCIFTVFIASSQGYFVFGKHMCAFEGFAGATG GLVTGWSLAFLAFERYIVICKPFGNFRFNSRHALLVVAATWIIGVGVAIPPFFGWS
>SWS1_Gekko  QTAFMGFVFFVGTPLNAIILFAIVKYKKLRQPLNYILVNISAAGFLFCVVAVFTVFISSSQGYFIFGKHICALEAFLGSLA GLVTGWSLAFLALERYIVICKPFGNFRFSAKHASLVVAATWFIGIGVSIPPYFGWS
>SWS1_Utasta QTAFMGFVFFAGTPLNAIILIVTVKYKKLRQPLNYILVNISFAGFLFCVFSVFTVFLASSQGYFFFGRHICALEAFLGSVA GLVTGWSLAFLAFERYIVICKPFGNFRFNSKHALLVVAATWFIGIGVSIPPFFGWS
>SWS1_Xenlae QAIFMGMVFLIGTPLNFIVLLVTIKYKKLRQPLNYILVNITVGGFLMCIFSIFPVFVSSSQGYFFFGRIACSIDAFVGTLT GLVTGWSLAFLAFERYIVICKPMGNFNFSSSHALAVVICTWIIGIVVSVPPFLGWS
>SWS1_Danio  QAAFMGFVFIVGTPMNGIVLFVTMKYKKLRQPLNYILVNISLAGFIFDTFSVSQVSVCAARGYYSLGYTLCSMEAAMGSIA GLVTGWSLAVLAFERYVVICKPFGSFKFGQGQAVGAVVFTWIIGTACATPPFFGWS
>SWS1_Oryzia QAAFMGFVFFVGTPLNFVVLLATAKYKKLRVPLNYILVNITFAGFIFVTFSVSQVFLASVRGYYFFGQTLCALEAAVGAVA GLVTSWSLAVLSFERYLVICKPFGAFKFGSNHALAAVIFTWFMGVGCACPPFFGWS
>SWS1_Geotri QAAFMGFVFICGTPLNAIVLVVTIKYKKLRQPLNYILVNISAAGLVFCLFSISTVFVASMQGYFFLGPTICALEAFFGSLA GLVTGWSLAFLAAERYIVICKPFGNFRFGSKHALVAVGLTWMLGLSVALPPFFGWS
>SWS2_ornAna LAAFMFLLITLGFPINLLTVICTIKYKKLRSHLNYILVNLAVSNMLVVCVGSATAFYSFAHMYFVLGPTACKIEGFAATLG GMVSLWSLAVIAFERFLVICKPLGNLSFRGTHAIFGCAATWVFGLAASLPPLFGWS
>SWS2_galGal MAAFMFLLIALGVPINTLTIFCTARFRKLRSHLNYILVNLALANLLVILVGSTTACYSFSQMYFALGPTACKIEGFAATLG GMVSLWSLAVVAFERFLVICKPLGNFTFRGSHAVLGCVATWVLGFVASAPPLFGWS
>SWS2_Taenio MAAFMFLLVLLGVPINALTVLCTAKYKKLRSHLNYILVNLAVANLLVVCVGSTTAFYSFSQMYFALGPLACKIEGFTATLG GMVSLWSLAVVAFERFLVICKPLGNFTFRGSHAVLGCAITWIFGLIASLPPLFGWS
>SWS2_Utasta MAAFMFLLIILGVPINVLTIFCTFKYKKLRSHLNYILVNLAVSNLLVVCIGSTTAFYSFAQMYFSLGPTACKIEGFAATLG GMVSLWSLAVVAFERFLVICKPLGNFSFRGTHAIIGCIITWVFGLVASLPPLFGWS
>SWS2_Xenopu ISAFMLFTIIFGFPLNLLTIICTVKYKKLRSHLNYILVNLAVANLIVICFGSTTAFYSFSQMYFSLGTLACKIEGFTATLG GIIGLWSLAVVAFERFLVICKPMGNFTFRESHAVLGCILTWVIGLVAAIPPLLGWS
>SWS2_Danio  MSAFMLFLFIAGTAINVLTIVCTIQYKKLRSHLNYILVNLAISNLWVSVFGSSVAFYAFYKKYFVFGPIGCKIEGFTSTIG GMVSLWSLAVVALERWLVICKPLGNFTFKTPHAIAGCILPWCMALAAGLPPLLGWS
>SWS2_Takifu MSAFMFFLFVAGTGINVLTIACTIQYKKLRSHLNYILVNLAFSNLLVTTVGSFTCFCCFFVRYMIVGPLGCKIEGFAATLG GMVSLWSLAVVAFERWLVVCKPLGNFIFKPDHAIVCCIFTWFFALIISAPPLFGWS
>SWS2_Geotri MSAFMLFLVLAGFPLNFLTVFVTIKYKKLRSHLNYILVNLAIANLIVVCCGSTLAFYSFMHKYFILGPLFCKMEGFTATLG GMLSLWSLAVLAFERCLVICKPFGNIAFRGTHALIRCGFAWAAAIAASTPPLFGWS
>LWS_ornAna  TSLWMIFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETLIASTISVINQIFGYFILGHPMCVLEGYTVSLC GITGLWSLSIISWERWIVVCKPFGNVKFDAKLAMVGIVFSWVWAAVWTAPPIFGWS
>LWS_galGal  TSLWMIFVVAASVFTNGLVLVATWKFKKLRHPLNWILVNLAVADLGETVIASTISVINQISGYFILGHPMCVVEGYTVSAC GITALWSLAIISWERWFVVCKPFGNIKFDGKLAVAGILFSWLWSCAWTAPPIFGWS
>LWS_anoCar  TSVWMIFVVIASIFTNGLVLVATAKFKKLRHPLNWILVNLAIADLGETVIASTISVINQISGYFILGHPMCVLEGYTVSTC GISALWSLAVISWERWVVVCKPFGNVKFDAKLAVAGIVFSWVWSAVWTAPPVFGWS
>LWS_Lithoch ATLWMFVVVVLSVFTNGLVLVATMKFKKLRHPLNWILVNLAIADLGETVFASTISVCNQFFGYFILGHPMCIFEGYVVSVC GIAALWSLTIISWERWIVVCKPFGNVKFDAKWATAGIVFSWVWAAVWCAPPIFGWS
>LWS_Gastero STLWMFIVVALSVFTNGLVLVATAKFKKLQHPLNWILVNLAIADLGETVFASTISVCNQFFGYFILGHPMCVFEGYVVSVC GITALWSLTIISWERWIVVCKPFGNVKFDAKWATAGIVFSWIWSAVWCAPPIFGWS
>LWS_Petrom  TSVWMIIVVVLSLFSNGLVLVATVKFKKLRHPLNWIIVNLAIADILETIFASTISVCNQVYGYFILGHPMCVFEGYVVSTC GIAGLWSLAIISWERWMVVCKPFGNIKFDGKIATILIVFSWVWPASWCSLPIFGWS
>LWS_lamprey TSVWMIIVVVLSLFTNGLVLVATMKFKKLRHPLNWILVNLAIADILETIFASTISVCNQVFGYFILGHPMCVFEGYVVSTC GIAGLWSLAIISWERWMVVCKPFGNIKFDGKIAIILIVFSWVWPACWCSLPIFGWS
>LWS_Geotria TSFWMIIVVILSLFTNGLVLVATLKFKKLRHPLNWILVNLAIADIGETIFASTVSVVNQIFGYFILGHPLCVFEGFTVSVC GITALWSLAIISFERWMVVCKPFGNLKFDGKVAIVLIIFSWAWSAGWCAPPIFGWS
>PIN_galGal  VAVLMGTVVACASVVNGLVIVVSICYKKLRSPLNYILVNLAVADLLVTLCGSSVSLSNNINGFFVFGRRMCELEGFMVSLT GIVGLWSLAILALERYVVVCRPLGDFQFQRRHAVSGCAFTWGWALLWSTPPLLGWS
>PIN_UtaSta  VAVLMGLVVVSAAFVNGLVIVVSIQYKKLRSPLNYILVNLAIADLLVTSFGSTLSFANNIYGFFVLGQTACEFEGFMVSLT GIVGLWSLAILAFERYLVICKPVGDFRFQQRHAVFGCVFTWMWSLVWTLPPLFGWS
>PIN_pheMad  LAALMGVVVLSASLANGLVIAVSVRFKRLRSPLNYILVNLATADLLVTFFGSIISFVNNAVGFFVFGKTACRFEGFMVSLT GIVGLWSLAILAFERYLVICKPVGDFQFQRRHAVIGCLYTWGWSLIWTVPPLFGWS
>PIN_podSic  VAVLMGLVVISATLVNGLVIVVSVQFKKLRSPLNYVLVNLAVADLLVTFFGSTISFVNNAQGFFIFGQATCEFEGFMVSLT GIVGLWSLAILAFERYLVICKPVGDFRFPARHAVLGCAFTWGWSFVWTVPPLLGWS
>PIN_xenTro  VAAVMCMVVILAFFVNGLVIVVTLKYKKLRSPLNYILVNLAIANLLVTIFGSSVSFSNNVVGYFFMGKTMCEFEGFMVSLT GIVGLWSLAILAFERYLVICKPMGDFRFQQKHAILGCSFTWVWSFIWTSPPLFGWC 
>PIN_bufJap  VAVLMGMVVFLAFFVNGMVIVVSLKYKKLRSPLNYILVNLAVADILVTMFGSTVSFHNNIFGFFTLGKLVCELEGFVVSLT GIVGLWSLAILAFERYIVICKPMGDFRFQQRHAVMGCAFTWIWAFLWTSPPLIGWC
>VAOP_galGal VAAVMFVVTSLSLAENLAVILVTFKFKQLRQPVNYVIVNLSVADFLVSLTGGTISFLANLKGYFYMGHWACVLEGFAVTFF GIVALWSLALLAFERYIVICRPVGNMRLRGKHAAQGIAFVWTFSFIWTIPPTMGWS
>VAOP_anoCar ISALMFVVTLFSLSENFTVILVTIKFKQLRQPLNYVIVNLSVADFLVSLIGGTISFSTNLKGYFYMGHWACVLEGFAVTFF GIVALWSLALLAFERYVVICRPLGNMRLNGKHAALGVAFVWIFSFIWTVPPTMGWS
>VAOP_xenTro LAALMFVVTSLSIAENFIVILVTAKFKQLRQPLNYIIVNLSVADFLVSVIGGTISIATNSRGYFYLGSWACVLEGFAVTFF GIVALWSLSVLAFERYIVICRPLGNLRLQGKHSALAIIFVWVFSFVWTIPPTMGWS
>VAOP_danRer LAALMFVVTALSLSENFTVMLVTFRFQQLRQPLNYIIVNLSLADFLVSLTGGSISFLTNYHGYFFLGKWACVLEGFAVTFF GIVALWSLAVLAFERFFVICRPLGNIRLRGKHAALGLVFVWSFSFIWTVPPVLGWS
>VAOP_rutRut LATLMFVVTAASLSENFAVMLVTFRFTQLRKPLNYIIVNLSLADFLVSLTGGTISFLTNYHGYFFLGKWACVLEGFAVTYF GIVALWSLAVLAFERFFVICRPLGNIRLRGKHAALGLLFVWTFSFIWTIPPVLGWS
>VAOP_Petro  LAALMGTITALSLGENFAVIVVTARFRQLRQPLNYVLVNLAAADLLVSAIGGSVSFFTNIKGYFFLGVHACVLEGFAVTYF GVVALWSLALLAFERYFVICRPLGNFRLQSKHAVLGLAVVWVFSLACTLPPVLGWS
>PPIN_anaCar IAIIMATSCTLSVILNTAVIAITIKYRQLRQPINYSLVNLAIADLGAALLGGSLNVETNAVGYYNLGRVGCVTEGFAMAFF GIVALCTIAVIAVDRAIVIAKPMGTITFTTRKAMIGVAVSWIWSLVWNTPPLFGWG
>PPIN_Xenop  LALIMAVFCAAALFLNVTVIVVTFKYRQLRHPINYSLVNLAIADLGVTVLGGALTVETNAVGYFNLGRVGCVIEGFAVAFF GIAALCTIAVIALDRVFVVCKPMGTLTFTPKQALAGIAASWIWSLIWNTPPLFGWG
>PPIN_Ictal  LSIIMALSSTFGIILNMVVIIVTVRYKQLRQPLNYALVNLAVADLGCPVFGGLLTAVTNAMGYFSLGRVGCVLEGFAVAFF GIAGLCSVAVIAVDRYMVVCRPLGAVMFQTKHALAGVVFSWVWSFIWNTPPLFGWG
>PPIN_Danio  LAVIIGVFSVCGVILNVTVITVTLKYKQLRQPLNFALVNLAVADLGCAVFGGLPTVVTNAMGYFSLGRVGCVLEGFAVAFF GIAALCSVAVIALERCMVVCRPVGSISFQTRHAVFGVAVSWLWSFIWNTPPLFGWG
>PPIN_Oncor  LAVIIGVFSVSGVCMNVLVIMVTMRHRKLRQPLNYALVNLAVADLGCALFGGLPTMVTNAMGYFSMGRLGCVLEGFAVAFF GIAGLCSVAVIAVDRYVVVCRPMGAVMFQTRHAVGGVVLSWVWSFLWNTPPLFGWG
>PPINa_Ciona LCVYMTFVFLLSCSLNILVIVATLKNKVLRQPLNYIIVNLAVVDLLSGFVGGFISIAANGAGYFFWGKTMCQIEGYFVSNF GVTGLLSIAVMAFERYFVICKPFGPVRFEEKHSIFGIVITWVWSMFWNTPPLIFWD
>PPINb_Ciona LAVYMTFIFLLAVSLNGFVIIATMKNKKLRQPLNYIIINLSIADFLSGLVGGFIGMISNSAGYFYFGKTVCILEGYIVSVA GVCGLMSISVMAFERYFVVCKPYGPFTLTNTHAALGIGFTWTWSVLWSTPGLIWLD
>PPIN_lamp   LAVIMAVFTIASLVLNSTVVIVTLRHRQLRHPLNFSLVNLAVADLGVTVFGASLVVETNAVGYFNLGRVGCVIEGFAVAFF GIAALCTIAVIAVDRFVVVCKPLGTLMFTRRHALLGIAWAWLWSFVWNTPPLFGWG
>PARIE_Utast LAFLMFLNALFSIFNNSLVIAVTLKNPQLRNPINIFILNLSFSDLMMSLCGTTIVIATNYYGYFYLGRKFCIFQGFAVNYF GIVSLWSLTILA YERYNVV--CQPLGTLQMSTKR GYQLLGFIWVFCLFWAVVPLFGWS
>PARIE_Anole LAFLMFINALFSLFNNFLVIAVTLKNPQLRNPINIFILNLSFSDLMMSICGTTIVIATNYHGYFYLGRRFCIFQGFAVNYF GIVSLWSLTILA YERYNVV--CQPLGTLQMSTQR AYQLLGFIWVFCLFWAVVPLFGWS
>PARIE_Xenop LSFLMFLNAVFSICNNAIVILVTLKHPQLRNPINIFILNLSFSDLMMALCGTTIVVSTNYHGYFYLGKQFCIFQGFAVNYF GIVSLWSLTLLA YERYNVV--CEPIGALKLSTKR GYQGLVFIWLFCLFWAIAPLFGWS
>ENCEPH_braB VAGVIAIIGVVGFVSNGAVVVLFLKFPQLRTPFNLLLLNMAVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANHLF GLVSLISLAVIS FLRYRMVVKPKGPGSSYLTYTK VGLAILFIYLYCLLWTTLPIAGWS
>ENCEPH_homS LALLLGSIGLLGVGNNLLVLVLYYKFQRLRTPTHLLLVNISLSDLLVSLFGVTFTFVSCLRNGWVWDTVGCVWDGFSGSLF GIVSIATLTVLA YERYIRVV-----HARVINFSW AWRAITYIWLYSLAWAGAPLLGWN
>ENCEPH_monD LALLIATIGLLGLCNNLLVLVLYYKFQRLRTPTHLFLVNISFNDLLVSLFGVTFTFVSCLRSGWVWDSVGCAWDGFSNTLF GIVSIMTLTVLA YERYNRIV-----HAKVINFSW AWRAITYIWLYSLVWTGAPLLGWN
>ENCEPH_galG LALLIATIGTLGVCNNLLVLVLYYKFKRLRTPTNLFLVNISLSDLLVSVCGVSLTFMSCLRSRWVWDAAGCVWDGFSNSLF GIVSIMTLTVLA YERYIRVV-----HAKVIDFSW SWRAITYIWLYSLAWTGAPLLGWN
>ENCEPH_anoC LALLVAAIGLLGLCNNLLVLVLYAKFKRLRTPTHLFLVNISLSDLLVSLFGVSFTFGSCLRHRWVWDAAGCVWDGFSNSLF GIVSIMTLTVLA YERYIRVV-----HARVIDFSW SWRAITYIWLYSLAWTGAPLLGWN
>ENCEPH_xenT LALIVATVGFLGLVNNLLVLILYCKFKRLQTPTNLLFFNTSLCHFVFSLLAITFTFMSCVRGSWAFSVEMCVFHGFSKNLL GIVSFGTLTVVA YERYARVV-----YGKYVNSSW SKRSITFVWVYSLAWTGFPLIGWN
>ENCEPH_braB IATGLALIGLVGSMNNFVVILLIGCHRQLRTPFNLLLLNVSVADLLVSVCGNTLSFASAVQHRWLWGRPGCVWYGFANSLF GIVSLVTLSALA FERYCVVV----RSSEMLTYKS SLGMIAFIWMYSLLWTSLPLLGWS
>ENCEPH_braF IATCLALIGFVGFTNNFVVILLIGCHRQLRTPFNLLLLNMSVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANSLF GIVSLVTLSALA FERYCVVV----RSSDMLTYKS SLVVITFIWLYSLLWTSLPLLGWS
>ENCEPH2_Api AIALGFIGFFGFTANLLVAIVIVKDAQILWTPVNVILFNLVFGDFLVSIFGNPVAMVSAATGGWYWGYKMCLWYAWFMSTL GFASIGNLTVMA VERWLLVA----RPMQALSIRH AVILASFVWIYALSLSLPPLFGWG
>ENCEPH1_Ano AAVTLFFIGFFGFFLNIFVIALMYKDVQLWTPMNIILFNLVCSDFSVSIIGNPLTLTSAISHRWLYGKSICVAYGFFMSLL GIASITTLTVLS YERFCLIS--RPFAAQNRSKQG ACLAVLFIWSYSFALTSPPLFGWG
>ENCEPH2_Ano SAVTLFFIGFFGFFLNLFVIALMCKDMQLWTPMNIILFNLVCSDFSVSIIGNPLTLTSAISHRWIFGRTLCVAYGFFMSLL GITSITTLTVLS YERYCLIS--RPFSSRNLTRRG AFLAIFFIWGYSFALTSPPLFGWG
>CILL2_Platy AIYLCIVGVIGTLSNGVIMYLYFKDKSLRSPMNLLFVNLAMSDFTVAFFGAMFQFGLTCTRKYMSPGMALCDFYGFITFLG GLASEMNLFIIS VERYLAVV--RPFDVGNLTNRR VIAGGVFVWLYSLVFAGGPLVGWS
>CILL1_Platy AAYLFFIACLGVSLNVLVLVLFIKDRKLRSPNNFLYVSLALGDLLVAVFGTAFKFIITARKTLLREEDGFCKWYGFITYLG GLAALMTLSVIA FVR CLAV-LRLGSFTGLTTRM GVAAMAFIWIYSLAFTLAPLLGWN
>MEL1_homSap LGTVILLVGLTGMLGNLTVIYTFCRSRSLRTPANMFIINLAVSDFLMSFTQAPVFFTSSLYKQWLFGETGCEFYAFCGALF GISSMITLTAIA LDRYLVIT-RPLATFGVASKRR AAFVLLGVWLYALAWSLPPFFGWS
>MEL1_smiCra IGATILVVGFTGVLGNLLVIYTFCRSRSLRTPANMFIINLAISDFFMSFTQAPVFFASSLYERWIFGEKGCEFYAFCGALF GITSMITLMVIA LDRYFVIT-RPLASIGMISKKK TGLILLGVWLYSLAWSLPPFFGWS
>MEL1_galGal IGTVILIVGITGTLGNFLVIYAFCRSRTLQKPANIFIINLAVSDFLMSITQSPVFFTNSLHKRWIFGEKGCELYAFCGALF GITSMITLMVIA LDRYFVIT-KPLASVRVMSKKK ALIILVGVWLYSLAWSLPPFFGWS
>MEL1_xenTro VGAVILAVGITGMLGNFLVIYAFCRSRSLRSPANMFIINLAITDFLMSVTQAPVFFATSLHKRWIFGEKGCELYAFCGALF GITSMITLMVIA VDRYFVIT-RPLTSIGVMSKKR AVLILSGVWLYSLAWSLPPFFGWS
>MEL1a_Bran  VGTAVFCIGCCGMFGNAVVVYSFIKSKGLRTPANFFIINLALSDFLMNLTNMPIFAVNSAFQRWLLSDFACELYGFAGGLF GCLSINTLMAIS MDRYLVIT-KPFLVMRIVTKQR VMFAILLLWIWSLVWALPPLFGWS
>MEL2_anoCar VGSCVLVIGCIGITGNLLVLYAFYSNKRLRTPPNYFIMNLAVSDFLMSATQAPICFLNSMHKEWVLGDIGCNLYAFCGALF GITSMITLLAIS VDRYCVIT-KPLQSIKRTSKKR TCIIIVFVWLYSLGWSVCPLFGWS
>MEL2_xenLae IGSFILIIGSVGIIGNMLVLYAFYRNKKLRTAPNYFIINLAISDFLMSATQAPVCFLSSLHREWILGDIGCNVYAFCGALF GITSMMTLLAIS INRYIVIT-KPLQSIQWSSKKR TSQIIVLVWMYSLMWSLAPLLGWS
>NEUR_homSap AGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFF GCGSLITMTAVS LDRYLKIC--YLSYGVWLKRKH AYICLAAIWAYASFWTTMPLVGLG
>RGR_homSap  VLLVEALSGLSLNTLTIFSFCKTPELRTPCHLLVLSLALADSGISLNALVAATSSLLRVSHRRWPYGSDGCQAHGFQGFVT ALASICSSAAIA WGRYHHYC-----TRSQLAWNS AVSLVLFVWLSSAFWAALPLLGWG
>PER_homSap  VATYLIMAGMISIISNIIVLGIFIKYKELRTPTNAIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYAGCQVYAGLNIFF GMASIGLLTVVA VDRYLTIC--LPDVGRRMTTNT YIGLILGAWINGLFWALMPIIGWA
>PERa_Branc  VGLYLFVIGIIGTIENGITLATFSKFRSLRSPTTMLLVHLAIADLGICIFGYPFSGASSLRSHWLFGGVGCQWYGFNGMFF  

>PPIN2_cioSa LCVFMSFVFVLAVPLNLLVIVATYKNKVLRRPINYIIVNLAVADLTCSVVGGLLGVLNNGAGYYFLGKSVCIFEGYVMSVT  
>MEL_Platy                                                                                     GIFGLMSINTLA WIAIDRFYVITNPLGAAQTMTKKRAFIILTIIWANASLWALAPFFGWG
>MEL1b_Bran  IFGSMMLVFGLIGVVGNVVALYAFCRTRSLRRPKNYVVANLCLTDMFVCLVYCPIVVSRSFSHGFPSKESCIVEGFMVGVG - 
>MEL2_galGal CFVNSLHREWILGDIGCDLYAFCGALFGITSMMTLLAISVDRYLVITKPLRSIQWTSKKRTIQIIAAVWLYSLGWS

amino terminal alignment of imaging opsins:
((((RHO1,RHO2), SWS1),(LWS,SWS2),(PIN,VAOP)
                                     !!!    ***   !!!     **  ***   * *  *
>RHO1_homSap                        MNGTEGPNFYVPFSNATGVVRSPFEYPQY-YLAEPWQFSM LAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG
>RHO1_monDom                        MNGTEGPNFYVPFSNKTGTVRSPFEEPQY-YLADPWQFSC LAAYMFMLIVLGFPINFLTLYVTIQHKKLRTPLNYILLNLAIADLFMVFGGFTMTLYTSLHGYFVFGPTGCNLEGFFATLG
>RHO1_ornAna                        MNGTEGQDFYIPMSNKTGVVRSPFEYPQY-YLAEPWQYSV LAAYMFMLIMLGFPINFLTLYVTIQHKKLRTPLNYILLNLAFANHFMVLGGFTTTLYTSLHGYFVFGPTGCNIEGFFATLG
>RHO1_galGal                        MNGTEGQDFYVPMSNKTGVVRSPFEYPQY-YLAEPWKFSA LAAYMFMLILLGFPVNFLTLYVTIQHKKLRTPLNYILLNLVVADLFMVFGGFTTTMYTSMNGYFVFGVTGCYIEGFFATLG
>RHO1_anoCar                        MNGTEGQNFYVPMSNKTGVVRNPFEYPQY-YLADPWQFSA LAAYMFLLILLGFPINFLTLFVTIQHKKLRTPLNYILLNLAVANLFMVLMGFTTTMYTSMNGYFIFGTVGCNIEGFFATLG
>RHO1_xenTro                        MNGTEGPNFYIPMSNKTGVVRSPFDYPQY-YLAEPWKYSA LAAYMFLLILLGFPINFMTLYVTIQHKKLRTPLNYILLNLVFANHFMVLCGFTVTMYTSMHGYFIFGQTGCYIEGFFATLG
>RHO1_danRer                        MNGTEGPAFYVPMSNATGVVRSPYEYPQY-YLVAPWAYGF VAAYMFFLIITGFPVNFLTLYVTIEHKKLRTPLNYILLNLAIADLFMVFGGFTTTMYTSLHGYFVFGRLGCNLEGFFATLG
>RHO1_Raja                          MNGTEGENFYVPMSNKTGVVRSPFDYPQY-YLGEPWMFSA LAAYMFFLILTGLPVNFLTLFVTIQHKKLRQPLNYILLNLAVSDLFMVFGGFTTTIITSMNGYFIFGPAGCNFEGFFATLG
>RHO1_lamp1                         MNGTEGENFYIPFSNKTGLARSPFEYPQY-YLAEPWKYSV LAAYMFFLILVGFPVNFLTLFVTVQHKKLRTPLNYILLNLAVANLFMVLFGFTLTMYSSMNGYFVFGPTMCNFEGFFATLG
>RHO1_lamp2                         MNGTEGQNFYIPFSNKTDVARSPFEYPQY-YLAEPWKFSA LAAYMFFLILVGFPVNFLTLFVTVQHKKLRTPLNYILLNLAVSNLFMILFGFTTTMYTSMNGYFVFGPTMCSIEGFFATLG
>RHO1_lamp3                         MNGTEGDNFYVPFSNKTGLARSPYEYPQY-YLAEPWKYSA LAAYMFFLILVGFPVNFLTLFVTVQHKKLRTPLNYILLNLAMANLFMVLFGFTVTMYTSMNGYFVFGPTMCSIEGFFATLG share second glyco, 2aa insert
>RHO2_galGal                        MNGTEGINFYVPMSNKTGVVRSPFEYPQY-YLAEPWKYRL VCCYIFFLISTGLPINLLTLLVTFKHKKLRQPLNYILVNLAVADLFMACFGFTVTFYTAWNGYFVFGPVGCAVEGFFATLG share second glyco, 2aa insert
>RHO2_anoCar                        MNGTEGINFYVPLSNKTGLVRSPFEYPQY-YLAEPWKYKV VCCYIFFLIFTGLPINILTLLVTFKHKKLRQPLNYILVNLAVADLFMACFGFTVTFYTAWNGYFIFGPIGCAIEGFFATLG
>RHO2_Gekko                         MNGTEGINFYVPLSNKTGLVRSPFEYPQY-YLADPWKFKV LSFYMFFLIAAGMPLNGLTLFVTFQHKKLRQPLNYILVNLAAANLVTVCCGFTVTFYASWYAYFVFGPIGCAIEGFFATIG
>RHO2_Lat                           MNGTEGMNFYVPLSNRTGLVRSPFEYTQY-YLAEPWKFSV LCAYMFLLIILGFPINFLTLLVTFKHKKLRQPLNYILVNLAVASLFMVVFGFTVTFYSSLNGYFVLGPMGCAMEGFFATLG
>RHO2_Geo                           MNGTEGANFYIPFHNRTGVVRSPYEYPQY-YLADPWMYSA ISAYVFTLILIGFPVNFMTLFVTFKLKKLRQPLNFILVNLCVADLLMIMFGFTTTFYTAMNGYFVFGPTGCNIEGFFATLG
>SWS1_homSap                       MRKMS-EEEFYL-FKNIS--SVGPWDGPQY-HIAPVWAFYL QAAFMGTVFLIGFPLNAMVLVATLRYKKLRQPLNYILVNVSFGGFLLCIFSVFPVFVASCNGYFVFGRHVCALEGFLGTVA 2aa indel is above
>SWS1_macDom                          MSGDEEFYL-FKNIS--SVGPWDGPQY-HIAPAWAFHF QTVFMGFVFCAGTPLNAVVLVATLRYKKLRQPLNYILVNVSLCGFIFCIFAVFTVFISSSQGYFIFGRHVCAMEAFLGSVA
>SWS1_galGal                          MSSDDDFYL-FTNGS--VPGPWDGPQY-HIAPPWAFYL QTAFMGIVFAVGTPLNAVVLWVTVRYKRLRQPLNYILVNISASGFVSCVLSVFVVFVASARGYFVFGKRVCELEAFVGTHG
>SWS1_Taeniop                         MD-EEEFYL-FKNQS--SVGPWDGPQY-HIAPMWAFYL QTIFMGLVFVAGTPLNAIVLIVTIKYKKLRQPLNYILVNISVSGLMCCVFCIFTVFIASSQGYFVFGKHMCAFEGFAGATG
>SWS1_Gekko                           MSGEEDFYL-YANIS--SVGPFDGPQY-HIAPMWAFYF QTAFMGFVFFVGTPLNAIILFAIVKYKKLRQPLNYILVNISAAGFLFCVVAVFTVFISSSQGYFIFGKHICALEAFLGSLA
>SWS1_Utasta                          MSGEEDFYL-FENIS--SVGPWDGPQY-HIAPMWAFYF QTAFMGFVFFAGTPLNAIILIVTVKYKKLRQPLNYILVNISFAGFLFCVFSVFTVFLASSQGYFFFGRHICALEAFLGSVA
>SWS1_Xenopus                         MLEEEDFYL-FKNVS--NVSPFDGPQY-HIAPKWAFTL QAIFMGMVFLIGTPLNFIVLLVTIKYKKLRQPLNYILVNITVGGFLMCIFSIFPVFVSSSQGYFFFGRIACSIDAFVGTLT
>SWS1_Danio                           MD--AWAVQ-FGNAS--KVSPFEGEQY-HIAPKWAFYL QAAFMGFVFIVGTPMNGIVLFVTMKYKKLRQPLNYILVNISLAGFIFDTFSVSQVSVCAARGYYSLGYTLCSMEAAMGSIA
>SWS1_Oryzias                         MG--KYFYL-YENIS--KVGPYDGPQY-YLAPTWAFYL QAAFMGFVFFVGTPLNFVVLLATAKYKKLRVPLNYILVNITFAGFIFVTFSVSQVFLASVRGYYFFGQTLCALEAAVGAVA
>SWS1_Geotri                          MSGDEEFYL-FKNIS--KVGPWDGPQF-HIAPKWAFYL QAAFMGFVFICGTPLNAIVLVVTIKYKKLRQPLNYILVNISAAGLVFCLFSISTVFVASMQGYFFLGPTICALEAFFGSLA
>SWS2_ornAna             MHKTHRNLQNELPEDFFIPLPLDTDNIT--SLSPFLVPQT-HLGGSGIFMS LAAFMFLLITLGFPINLLTVICTIKYKKLRSHLNYILVNLAVSNMLVVCVGSATAFYSFAHMYFVLGPTACKIEGFAATLG
>SWS2_galGal             MH--PPRPTTDLPEDFYIPMALDAPNIT--ALSPFLVPQT-HLGSPGLFRA MAAFMFLLIALGVPINTLTIFCTARFRKLRSHLNYILVNLALANLLVILVGSTTACYSFSQMYFALGPTACKIEGFAATLG
>SWS2_Taenio              MPKPREMRDELPEDFYIPMSLETPNLT--ALSPFLVPQT-HLGSPGIFKA MAAFMFLLVLLGVPINALTVLCTAKYKKLRSHLNYILVNLAVANLLVVCVGSTTAFYSFSQMYFALGPLACKIEGFTATLG
>SWS2_Utasta             MHNSRPHSRDDLPEDFFIPMPLDVANIT--TLSPFLVPQT-HLGSPALFMG MAAFMFLLIILGVPINVLTIFCTFKYKKLRSHLNYILVNLAVSNLLVVCIGSTTAFYSFAQMYFSLGPTACKIEGFAATLG
>SWS2_Xenopu             MSKGRPDLRMEMPDEFYVPIPLETTNIS--SLSPFLVPQT-HLGTPGIFMS ISAFMLFTIIFGFPLNLLTIICTVKYKKLRSHLNYILVNLAVANLIVICFGSTTAFYSFSQMYFSLGTLACKIEGFTATLG
>SWS2_Danio                MKQQQQTPELFEDFHMPITLDVSNIS--AYSPFLVPQD-HLGHSGVFMG MSAFMLFLFIAGTAINVLTIVCTIQYKKLRSHLNYILVNLAISNLWVSVFGSSVAFYAFYKKYFVFGPIGCKIEGFTSTIG
>SWS2_Takifu                MRGVRQHEFQEDFYIPIPLDVDNIT--ALSPFLVPQD-HLGSPAVFYG MSAFMFFLFVAGTGINVLTIACTIQYKKLRSHLNYILVNLAFSNLLVTTVGSFTCFCCFFVRYMIVGPLGCKIEGFAATLG 
>SWS2_Geotri             MYQGKSTQVDDLPEDFYIPIALNVKNMS--ELSPFLVPQV-HLGDSFIFYG MSAFMLFLVLAGFPLNFLTVFVTIKYKKLRSHLNYILVNLAIANLIVVCCGSTLAFYSFMHKYFILGPLFCKMEGFTATLG
>LWS_ornAna      MTPAWNSGVYAARRRFEDEEDTTRTSVFVYTNSNNT---RDPFEGPNY-HIAPRWAYNV TSLWMIFVVIASVFTNGLVLVATMKFKKLRHPLNWILVNLAVADLGETLIASTISVINQIFGYFILGHPMCVLEGYTVSLC 1 aa indel
>LWS_galGal      MAAWEA--AFAARRR-HEEEDTTRDSVFTYTNSNNT---RGPFEGPNY-HIAPRWVYNL TSLWMIFVVAASVFTNGLVLVATWKFKKLRHPLNWILVNLAVADLGETVIASTISVINQISGYFILGHPMCVVEGYTVSAC
>LWS_anoCar  MAGTVTEAWDVAVFAARRR-NDEDDTTRDSLFTYTNSNNT---RGPFEGPNY-HIAPRWVYNI TSVWMIFVVIASIFTNGLVLVATAKFKKLRHPLNWILVNLAIADLGETVIASTISVINQISGYFILGHPMCVLEGYTVSTC
>LWS_Lithoch     MAEEWGKQSFAARRY---HEDSTRGSAFAYTNSNNT---RDPFEGPNY-HIAPRWIYNL ATLWMFVVVVLSVFTNGLVLVATMKFKKLRHPLNWILVNLAIADLGETVFASTISVCNQFFGYFILGHPMCIFEGYVVSVC
>LWS_Gastero     MAEEWGKQAFAARRY---NEDTTRGSMFVYTNSNNT---KDPFEGPNY-HIAPRWVYNL STLWMFIVVALSVFTNGLVLVATAKFKKLQHPLNWILVNLAIADLGETVFASTISVCNQFFGYFILGHPMCVFEGYVVSVC
>LWS_Petrom      MTASWQGAMFAARRR-QDDEDTTMESLFRYTNENNT---KDPFEGPNY-HIAPRWVFNL TSVWMIIVVVLSLFSNGLVLVATVKFKKLRHPLNWIIVNLAIADILETIFASTISVCNQVYGYFILGHPMCVFEGYVVSTC
>LWS_lamprey     MTASWHGAVFAARRR-NDDEDTTKDSIFRYTNENNT---RDPFEGPNY-HIAPRWMFNL TSVWMIIVVVLSLFTNGLVLVATMKFKKLRHPLNWILVNLAIADILETIFASTISVCNQVFGYFILGHPMCVFEGYVVSTC
>LWS_Geotria     MAQSWERAMFAARRR--QDEDTTKGDLFRYTNENNT---RDPFEGPNY-HIAPRWMYNL TSFWMIIVVILSLFTNGLVLVATLKFKKLRHPLNWILVNLAIADIGETIFASTVSVVNQIFGYFILGHPLCVFEGFTVSVC
>PIN_galGal                             MSSNSSQAPPNGT---PGPFDGPQWPYQAPQSTYVG VAVLMGTVVACASVVNGLVIVVSICYKKLRSPLNYILVNLAVADLLVTLCGSSVSLSNNINGFFVFGRRMCELEGFMVSLT P 1aa insert non-ancestral unless VAOP earlier
>PIN_utaSta                                 MVNEWSNAT---PGPFDGPQWPYLAPRSIYTS VAVLMGLVVVSAAFVNGLVIVVSIQYKKLRSPLNYILVNLAIADLLVTSFGSTLSFANNIYGFFVLGQTACEFEGFMVSLT these opsins are very limited frog to liz/bird
>PIN_pheMad                         MHVQMANASQASLKNGT---LSPFDGPQWPHRASRRVYTS LAALMGVVVLSASLANGLVIAVSVRFKRLRSPLNYILVNLATADLLVTFFGSIISFVNNAVGFFVFGKTACRFEGFMVSLT
>PIN_podSic                           MQASNASWVEVRNRT---PGPFEGPQWPYLAPQSTYIS VAVLMGLVVISATLVNGLVIVVSVQFKKLRSPLNYVLVNLAVADLLVTFFGSTISFVNNAQGFFIFGQATCEFEGFMVSLT
>PIN_xenTro                               MRAGNMSAYEA---PGPYDGPQWPHLAPRSTFLT VAAVMCMVVILAFFVNGLVIVVTLKYKKLRSPLNYILVNLAIANLLVTIFGSSVSFSNNVVGYFFMGKTMCEFEGFMVSLT the glyco is either not homologous or lost in frogs
>PIN_bufJap                               MHSANMSALET---PGPFEGPQWPHVAPRSTYLT VAVLMGMVVFLAFFVNGMVIVVSLKYKKLRSPLNYILVNLAVADILVTMFGSTVSFHNNIFGFFTLGKLVCELEGFVVSLT
>VAOP_galGal                       MDVFRALGNESLLSNSSGPARWDPFHHPLD-SIQP-WHFRL VAAVMFVVTSLSLAENLAVILVTFKFKQLRQPVNYVIVNLSVADFLVSLTGGTISFLANLKGYFYMGHWACVLEGFAVTFF no consistent glyco, much older than PIN
>VAOP_anoCar                        MAGLRREAENDSWLFDPSSSSAPFLQPLD-IIEP-WNFHL ISALMFVVTLFSLSENFTVILVTIKFKQLRQPLNYVIVNLSVADFLVSLIGGTISFSTNLKGYFYMGHWACVLEGFAVTFF losing all the invariant residues almost
>VAOP_xenTro                            MPTNVSLLATPENSTVWNPFTGPLK-TIEA-WNFHL LAALMFVVTSLSIAENFIVILVTAKFKQLRQPLNYIIVNLSVADFLVSVIGGTISIATNSRGYFYLGSWACVLEGFAVTFF
>VAOP_danRer                              MEASSAAVNAVSPAEDPFSAPLS-SIAP-WNYSV LAALMFVVTALSLSENFTVMLVTFRFQQLRQPLNYIIVNLSLADFLVSLTGGSISFLTNYHGYFFLGKWACVLEGFAVTFF
>VAOP_rutRut                              MELFPVAVNGVSHAEDPFSGPLT-FIAP-WNYKV LATLMFVVTAASLSENFAVMLVTFRFTQLRKPLNYIIVNLSLADFLVSLTGGTISFLTNYHGYFFLGKWACVLEGFAVTYF
>VAOP_Petro              MDALQESPPSHHSLPSALPSATGGNGTVATMHNPFERPLE-GIAP-WNFTM LAALMGTITALSLGENFAVIVVTARFRQLRQPLNYVLVNLAAADLLVSAIGGSVSFFTNIKGYFFLGVHACVLEGFAVTYF
>PPIN_anaCar                                    MDSLDTNTLSPNASTVRVVLMPRIGYTI IAIIMATSCTLSVILNTAVIAITIKYRQLRQPINYSLVNLAIADLGAALLGGSLNVETNAVGYYNLGRVGCVTEGFAMAFF
>PPIN_Xenop                                 MADEALLPPMMNVTNEEMHPGKVLMPRIGYTI LALIMAVFCAAALFLNVTVIVVTFKYRQLRHPINYSLVNLAIADLGVTVLGGALTVETNAVGYFNLGRVGCVIEGFAVAFF
>PPIN_Ictal                                 MASIILINFSETDTLHLGSVNDHIMPRIGYTI LSIIMALSSTFGIILNMVVIIVTVRYKQLRQPLNYALVNLAVADLGCPVFGGLLTAVTNAMGYFSLGRVGCVLEGFAVAFF
>PPIN_Danio                                        MESETSTAASGSIAE-VMPRMGYTI LAVIIGVFSVCGVILNVTVITVTLKYKQLRQPLNFALVNLAVADLGCAVFGGLPTVVTNAMGYFSLGRVGCVLEGFAVAFF
>PPIN_Oncor                                MDHQQLLPNLHGNISSSPGSVSEALLSRTGFTI LAVIIGVFSVSGVCMNVLVIMVTMRHRKLRQPLNYALVNLAVADLGCALFGGLPTMVTNAMGYFSMGRLGCVLEGFAVAFF
>PPINa_Ciona                  MDHDVTPTVDLTDGVPQCKDLNPYVLKGDGWVPQHISRANRSTYSF LCVYMTFVFLLSCSLNILVIVATLKNKVLRQPLNYIIVNLAVVDLLSGFVGGFISIAANGAGYFFWGKTMCQIEGYFVSNF
>PPINb_Ciona                MTTAETTTECYEKNPYIRNEMGWVPKHILIAERHIYTILAVYMTFIFL LAVYMTFIFLLAVSLNGFVIIATMKNKKLRQPLNYIIINLSIADFLSGLVGGFIGMISNSAGYFYFGKTVCILEGYIVSVA
>PPIN2_cioSa                               IPQNYSNGNPYATTDSGWVPEHIEIANRSTYSG LCVFMSFVFVLAVPLNLLVIVATYKNKVLRRPINYIIVNLAVADLTCSVVGGLLGVLNNGAGYYFLGKSVCIFEGYVMSVT
>PPIN_lamp                                         MENLTSLDLLPNGEVPLMPRYGFTI LAVIMAVFTIASLVLNSTVVIVTLRHRQLRHPLNFSLVNLAVADLGVTVFGASLVVETNAVGYFNLGRVGCVIEGFAVAFF 
>PARIE_Utast       ME-NDSSLATEL--------------------AEGAIVKPTIFPKAG-------YGV LAFLMFLNALFSIFNNSLVIAVTLKNPQLRNPINIFILNLSFSDLMMSLCGTTIVIATNYYGYFYLGRKFCIFQGFAVNYF 
>PARIE_Anole       ME--NESSLVLE--------------------AEGYIVRPTIFPRAG-------YGV LAFLMFINALFSLFNNFLVIAVTLKNPQLRNPINIFILNLSFSDLMMSICGTTIVIATNYHGYFYLGRRFCIFQGFAVNYF 
>PARIE_Xenop       MD-GNSTTPGI---------------------AVNLTVMPTIFPRSG-------YSI LSFLMFLNAVFSICNNAIVILVTLKHPQLRNPINIFILNLSFSDLMMALCGTTIVVSTNYHGYFYLGKQFCIFQGFAVNYF 
>ENCEPH_braBe       EY-GNNTTFAAWDFKRNGTGGEEEVEF-----------------FG-------YDA VAGVIAIIGVVGFVSNGAVVVLFLKFPQLRTPFNLLLLNMAVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANHLF 
>ENCEPH_homS       MYSGNRS--------GGHGYWDGGGAAGAEG------PAPAGTLSPAPLFSPGTYER LALLLGSIGLLGVGNNLLVLVLYYKFQRLRTPTHLLLVNISLSDLLVSLFGVTFTFVSCLRNGWVWDTVGCVWDGFSGSLF 
>ENCEPH_monD       MYSDNSS------DDGGGGYWGSGRAGGASGTGVTGEPGPEGSPRQAPLFSPGTYEL LALLIATIGLLGLCNNLLVLVLYYKFQRLRTPTHLFLVNISFNDLLVSLFGVTFTFVSCLRSGWVWDSVGCAWDGFSNTLF 
>ENCEPH_galG       MHSGNGT---------------------GATSRPQLAAAGHEVPGERPLFSAGTYEL LALLIATIGTLGVCNNLLVLVLYYKFKRLRTPTNLFLVNISLSDLLVSVCGVSLTFMSCLRSRWVWDAAGCVWDGFSNSLF 
>ENCEPH_anoC       MFSANGT---------RSGAGSDLEPGPGQQQQQREASEEEERGAGLSPFSAGTYEL LALLVAAIGLLGLCNNLLVLVLYAKFKRLRTPTHLFLVNISLSDLLVSLFGVSFTFGSCLRHRWVWDAAGCVWDGFSNSLF 
>ENCEPH_xenT       MPVTNGS-----------------------------HNNSISWLHSKDMFTEDTYHF LALIVATVGFLGLVNNLLVLILYCKFKRLQTPTNLLFFNTSLCHFVFSLLAITFTFMSCVRGSWAFSVEMCVFHGFSKNLL 
>ENCEPH_braB       MPLYNTSSGPTQGLPWDTPYSQDPIWNDSSPSNSSEDAVVDQGRGELQDFSDAGYTA IATGLALIGLVGSMNNFVVILLIGCHRQLRTPFNLLLLNVSVADLLVSVCGNTLSFASAVQHRWLWGRPGCVWYGFANSLF 
>ENCEPH_braF       MALYNNTSSPSQDLLWDAPYSQGHIW-DNSSASNSSEDVMDQGKVELQDFSDAGYTA IATCLALIGFVGFTNNFVVILLIGCHRQLRTPFNLLLLNMSVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANSLF 
>ENCEPH2_Api                                              MEHVIYEDQVSPVMYIGA AIALGFIGFFGFTANLLVAIVIVKDAQILWTPVNVILFNLVFGDFLVSIFGNPVAMVSAATGGWYWGYKMCLWYAWFMSTL 
>ENCEPH1_Ano                                      MYDVTDAAAINSDHQELMAPWAYNGA AVTLFFIGFFGFFLNIFVIALMYKDVQ-LWTPMNIILFNLVCSDFSVSIIGNPLTLTSAISHRWLYGKSICVAYGFFMSLL 
>ENCEPH2_Ano                                     MNDAPNDVAASAVDYEDLMAPWAYNAS AVTLFFIGFFGFFLNLFVIALMCKDMQ-LWTPMNIILFNLVCSDFSVSIIGNPLTLTSAISHRWIFGRTLCVAYGFFMSLL 
>CILL2_Platyr                        MDDLGFLGNSSVNYTVPLLQEDPLLLRILYFGPTSYVIT AIYLCIVGVIGTLSNGVIMYLYFKDKSLRSPMNLLFVNLAMSDFTVAFFGAMFQFGLTCTRKYMSPGMALCDFYGFITFLG 
>CILL1_Platyr            MDGENLTIPNPVTELMDTPINSTYFQNLNAETDGGNHYIYNAFTATDYNIC AAYLFFIACLGVSLNVLVLVLFIKDRKLRSPNNFLYVSLALGDLLVAVFGTAFKFIITARKTLLREEDGFCKWYGFITYLG
                                     !!!    ***   !!!     **  ***   * *  *
>RHO1_homSap                        MNGTEGPNFYVPFSNATGVVRSPFEYPQY-YLAEPWQFSM LAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG

Here's the distal alignment of the opsins:


>RHO1_homSap   RYIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKE AAAQQQESATTQKAEK----EVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQ FRNCMLTTIC CGKNPLG DDEASATVS  KTE     TSQVAPA
>RHO1_monDom   RYIPEGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPLIVIFFCYGQLVFTVKE AAAQQQESATTQKAEK----EVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSNFGPIFMTIPAFFAKSSSVYNPVIYIMMNKQ FRTCMITTLC CGKNPLG DDEASATAS  KTE     TSQVAPA
>RHO1_ornAna   RYIPEGMQCSCGIDYYTLRPEVNNESFVIYMFVVHFTIPMTIIFFCYGRLVFTVKE AAAQQQESATTQKAEK----EVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTVPAFFAKSSAIYNPVIYIMMNKQ FRNCMLTTIC CGKNPLG DDEASATAS  KTEQSSVSTSQVSPA
>RHO1_galGal   RYIPEGMQCSCGIDYYTLKPEINNESFVIYMFVVHFMIPLAVIFFCYGNLVCTVKE AAAQQQESATTQKAEK----EVTRMVIIMVIAFLICWVPYASVAFYIFTNQGSDFGPIFMTIPAFFAKSSAIYNPVIYIVMNKQ FRNCMITTLC CGKNPLG DEDTSAG    KTETSSVSTSQVSPA
>RHO1_anoCar   RYIPEGMQCSCGVDYYTPTPEVHNESFVIYMFLVHFVTPLTIIFFCYGRLVCTVKA AAAQQQESATTQKAER----EVTRMVVIMVISFLVCWVPYASVAFYIFTHQGSDFGPVFMTIPAFFAKSSAIYNPVIYILMNKQ FRNCMIMTLC CGKNPLG DEDTSAGT   KTETSTVSTSQVSPA
>RHO1_xenTro   RYIPEGMQCSCGVDYYTLKPEVNNESFVVYMFIVHFTIPLCVIFFCYGRLLCTVKE AAAQQQESATTQKAEK----EVTRMVVMMVIFFLICWVPYAYVAFYIFTHQGSDFGPVFMTVPAFFAKSSAIYNPVIYIVLNKQ FRNCLITTLC CGKNPFG DEEGSSAASS KTEASSVSSSQVSPA
>RHO1_danRer   RYIPEGMQCSCGVDYYTRTPGVNNESFVIYMFIVHFFIPLIVIFFCYGRLVCTVKE AARQQQESETTQRAER----EVTRMVIIMVIAFLICWLPYAGVAWYIFTHQGSEFGPVFMTLPAFFAKTSAVYNPCIYICMNKQ FRHCMITTLC CGKNPFE EEEGASTTAS KTEASSVSSSSVSPA
>RHO1_Raja     RYIPEGLQCSCGVDYYTMKPEVNNESFVIYMFVVHFTIPLIVIFFCYGRLVCTVKE AAAQQQESESTQRAER----EVTRMVIIMVVAFLICWVPYASVAFYIFINQGCDFTPFFMTVPAFFAKSSAVYNPLIYILMNKQ FRNCMITTIC LGKNPFE EEESTSASAS KTEASSVSSSQVAPA
>RHO1_lamprey1 RYLPEGMQCSCGPDYYTLNPNFNNESFVIYMFLVHFIIPFIVIFFCYGRLLCTVKE AAAAQQESASTQKAEK----EVTRMVVLMVIGFLVCWVPYASVAFYIFTHQGSDFGATFMTVPAFFAKTSALYNPIIYILMNKQ FRNCMITTLC CGKNPLG DEDSGASTS  KTEVSSVSTSQVSPA
>RHO1_lamprey2 RYLPEGMQCSCGPDYYTMNPTYNNESFVIYMFIVHFTIPFVIIFFSYGRLLCTVKE AAAAQQESASTQKAEK----EVTRMVVLMVVGFLVCWVPYASVAFYIFTNQGSDFGATFMTLPAFFAKSSALYNPVIYILMNKQ FRNCMITTLC CGKNPLG DDDSGASTS  KTEVSSVSTSQVAPA
>RHO1_lamprey3 RYIPEGMQCSCGPDYYTLNPNFNNESYVVYMFVVHFLVPFVIIFFCYGRLLCTVKE AAAAQQESASTQKAEK----EVTRMVVLMVIGFLVCWVPYASVAFYIFTHQGSDFGATFMTLPAFFAKSSALYNPVIYILMNKQ FRNCMITTLC CGKNPLG DDESGASTS  KTEVSSVSTSQVSPA
>RHO2_galGal   RYMPEGMQCSCGPDYYTHNPDYHNESYVLYMFVIHFIIPVVVIFFSYGRLICKVRE AAAQQQESATTQKAEK----EVTRMVILMVLGFMLAWTPYAVVAFWIFTNKGADFTATLMAVPAFFSKSSSLYNPIIYVLMNKQ FRNCMITTIC CGKNPFG DEDVSSTVSQSKTEVSSVSSSQVSPA
>RHO2_anoCar   RYIPEGMQCSCGPDYYTLNPDYHNESYVLYMFGVHFVIPVVVIFFSYGRLICKVRE AAAQQQESASTQKAER----EVTRMVILMVLGFLLAWTPYAMVAFWIFTNKGVDFSATLMSVPAFFSKSSSLYNPIIYVLMNKQ FRNCMITTIC CGKNPFG DEDVSSSVSQSKTEVSSVSSSQVSPA
>RHO2_Gekko    RFIPEGMQCSCGPDYYTLNPDFHNESYVIYMFIVHFTVPMVVIFFSYGRLVCKVRE AAAQQQESATTQKAEK----EVTRMVILMVLGFLLAWTPYAATAIWIFTNRGAAFSVTFMTIPAFFSKSSSIYNPIIYVLLNKQ FRNCMVTTIC CGKNPFG DEDVSSSVSQSKTEVSSVSSSQVAPA
>RHO2_Latime   RYIPEGLQCSCGPDYYTLNPDFHNESYVMYLFLVHFLLPIIIIFFTYGRLICKVKE AAAQQQESASTQKAEK----EVTRMVILMVIGFLTAWVPYASAAFWIFCNRGAEFTATLMTVPAFFSKSSCLFNPIIYVLLNKQ FRNCMITTLC CGKNPLG DDDTSSAVSQSKTDVSSVSSSQVSPA
>RHO2_Geot     RYIPEGMQCSCGPDYYTLNPKYYNESYVIYLFLVHFLLPVTIIFFTYGRLICTVKE AAAQQQESASTQKAER----EVTRMVIIMVVGFLVCWVPYASFAFYLFMNKGILFSATAMTVPAFFSKSSVLYNPIIYVLLNKQ FRTCMVTTLF CGKNPFG EDDSSMVSTS KTEVSSVSSSQVSPS
>SWS1_homSap   RFIPEGLQCSCGPDWYTVGTKYRSESYTWFLFIFCFIVPLSLICFSYTQLLRALKA VAAQQQESATTQKAER----EVSRMVVVMVGSFCVCYVPYAAFAMYMVNNRNHGLDLRLVTIPSFFSKSACIYNPIIYCFMNKQ FQACIMKMV  CGKAMTDESDTCSSQ    KTEVSTVSSTQVGPN
>SWS1_macDom   RFIPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIMPLFLICFSYSQLLRALRA VAAQQQESATTQKAER----EVSRMVVMMVGSFCLCYVPYAALAMYMVNNQNHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ FHACIMEMV  CRKPMTDDSDVSSSQ    KTEVSAVSSSQVGPT
>SWS1_galGal   RYMPEGLQCSCGPDWYTVGTKYRSEYYTWFLFIFCFIVPLSLIIFSYSQLLSALRA VAAQQQESATTQKAER----EVSRMVVVMVGSFCLCYVPYAALAMYMVNNRDHGLDLRLVTIPAFFSKSACVYNPIIYCFMNKQ FRACIMETV  CGKPLTDDSDASTSAQ   RTEVSSVSSSQVGPT
>SWS1_Taeni    RYIPEGLQCSCGPDWYTVGTKYKSEYYTWFLFIFCFIVPLSLIIFSYSQLLSALRA VAAQQQESATTQKAER----EVSRMVVVMVGSFCMCYVPYAALAMYMVNNREHGIDLRLVTIPAFFSKSSCVYNPIIYCFMNKQ FRACIMETV  CGRPMTDDSEVSSSAQ   RTEVSSVSSSQVGPS
>SWS1_Gekko    RFIPEGLQCSCGPDWYTVGTKYYSEYYTWFLFVLCFIVPLSIIVFSYSQLLSALRA VAAQQQESATTQKAER----EVSRMVVVMVGSFCLCYVPYAALAMYMVNNRNHGIDLRMVTIPAFFSKSSCVYNPIIYCFMNKQ FRGCILEMV  CGKTMAEESEVSSASQ   KTEVSSVSSSQVGPS
>SWS1_Uta      RFIPEGLQCSCGPDWYTVGTKYKSEYYTWFLFIFCFIVPLTLIIFSYSQLLGALRA VAAQQQESATTQKAER----EVSRMVVVMVGSFCLCYVPYAALAMYMVNNRDHGIDLRLVTIPAFFSKSACVYNPIIYCFMNKQ FRACIMETV  CGKPMTDESDVSSSAQ   KTEVSSVSSSQVSPS
>SWS1_Xenop    RYMPEGLQCSCGPDWYTVGTKYRSEYYTWFIFIFCFVIPLSLICFSYGRLLGALRA VAAQQQESASTQKAER----EVSRMVIFMVGSFCLCYVPYAAMAMYMVTNRNHGLDLRLVTIPAFFSKSSCVYNPIIYSFMNKQ FRGCIMETV  CGRPMSDDSSVSSTSQ   RTEVSTVSSSQVSPA
>SWS1_Danio    RYIPEGLGCSCGPDWYTNCEEFSCASYSKFLLVTCFICPITIIIFSYSQLLGALRA VAAQQAESASTQKAEK----EVSRMIIVMVASFVTCYGPYALTAQYYAYSQDENKDYRLVTIPAFFSKSSCVYNPLIYAFMNKQ FNACIMETV  FGKKIDESSEVSS      KTETSSVSA
>SWS1_Oryzia   RYIPEGLQCSCGPDWYTVGTKYKSEYYTYFLFVFCFVVPLSIIIFSYGSLLGTLRA VAAQQQESASTQKAER----EVSRMVIMMVASFCTCYVPYAALAVYMVTNRDHNIDLRFVTVPAFFSKASCVYNPLIYSFMNKQ FNGCIMEMV  FGKKMEEASEVSS      KTEVSTDS
>SWS1_Geotri   RYIPEGLQCSCGPDWYTVGTKYKSEYYTYFLFVFCFVVPLSIIIFSYGSLLGTLRA VAAQQQESASTQKAER----EVSRMVIMMVASFCTCYVPYAALAVYMVTNRDHNIDLRFVTVPAFFSKASCVYNPLIYSFMNKQ FRACILETV  CGKPITDESETSSS     RTEVSSVSTTQMIPG
>SWS2_ornAna   RYIPEGLQCSCGPDWYTTNNKWNNESYVIFLFSFCFGVPLSIIIFSYGRLLLTLRA VAKQQEQSATTQKAER----EVTKMVIVMVLGFLVCWLPYASFSLWVVTNRGQVFDLRMASIPSVFSKASTIYNPIIYVFMNKQ FRSCMLKLVF CGKSPFGDEDEISGSS   QATQVSSVSSSQVSPA
>SWS2_galGal   RYIPEGLQCSCGPDWYTTDNKWHNESYVLFLFTFCFGVPLAIIVFSYGRLLITLRA VARQQEQSATTQKADR----EVTKMVVVMVLGFLVCWAPYTAFALWVVTHRGRSFEVGLASIPSVFSKSSTVYNPVIYVLMNKQ FRSCMLKLLF CGRSPFGDDEDVSGSS   QATQVSSVSSSHVAPA
>SWS2_Taeni    RYIPEGLQCSCGPDWYTTDNKWNNESYVIFLFCFCFGFPLTVIVFSYGRLLLTLRA VAKQQEQSASTQKAER----EVTKMVVVMVLGFLVCWLPYCSFALWVVTHRGHPFDLGLASIPSVFSKASTVYNPIIYVFMNKQ FRSCMLKLVF CGRSPFGDEDDVSGSS   QATQVSSVSSSQVSPA
>SWS2_Uta      RYIPEGLQCSCGPDWYTTNNKWNNESYVLFLFSFCFGVPLSVIIFSYGRLLLTLRA VAKQQEQSATTQKAER----EVTKMVVVMVMGFLVCWLPYASFALWVVTHRGEPFDVRLATIPSVFSKASSVYNPVIYVFMNKQ FRSCMLKLVF CGKSPFGDEDDVSGSS   QTTQVSSVSSSQVSPA
>SWS2_Xeno     RYIPEGLQCSCGPDWYTVNNKWNNESYVLFLFCFCFGFPLAIIVFSYGRLLLALHA VAKQQEQSATTQKAER----EVTRMVIVMVVGFLVCWLPYASFALWAVTHRGELFDLRMSSVPSVFSKASTVYNPFIYIFMNRQ FRSCMMKMIF CGKNPLGDDEETSVSG    STQVSSVSSSQIAPS
>SWS2_Danio    RYIPEGLQCSCGPDWYTTNNKFNNESYVMFLFCFCFAVPFSTIVFCYGQLLITLKL AAKAQADSASTQKAER----EVTKMVVVMVFGFLICWGPYAIFAIWVVSNRGAPFDLRLATIPSCLCKASTVYNPVIYVLMNKQ FRSCMMKMVF NKNIEEDEASSSS      QVTQVSSVAPEK
>SWS2_Takif    RYIPEGFQCSCGPDWYTTGNKYNNESYVWFIFGFGFAVPLFVIVFCYSQLLVMLKS AAKAQAESASTQKAER----EVTRMVVVMILGFLVCWLPYASFALWVVNNRGTPFDLRLATIPACFSKASTVYNPIIYVVLNKQ FRSCMKKMLG MSGGDDEESSS          QSVTEVSKVSPS
>SWS2_Geotria  RYIPEGLQCSCGPDWYTTNNKYNNESYVMFLFIFCFGTPFTIIIVSYSKLILTLRA AAAQQQESASTQKAEK----EVSRMVVIMVGGFLVCWLPYASLALWIVFNRGSPFDLRLATIPSVFSKASTVYNPVIYIFLNKQ FRSCMMKTIF CGKNPLGDDEDATSTTT    QVSSVSTSQVAPA
>LWS_ornAna    RYWPHGLKTSCGPDVFSGSSDPGVQSYMIVLMSTCCILPLSIIVLCYLQVWLAIRA VAKQQKESESTQKAEK----EVSRMVVVMILAYCFCWGPYTIFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ FRNCIMQL   FGKKVDDGSELSSTS    RTEVSSVSS  VSPA
>LWS_galGal    RYWPHGLKTSCGPDVFSGSSDPGVQSYMVVLMVTCCFFPLAIIILCYLQVSLAIRA VAAQQKESESTQKAEK----EVSRMVVVMIVAYCFCWGPYTFFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ FRNCILQL   FGKKVDDGSEVSTS     RTEVSSVSNSSVSPA
>LWS_anoCar    RYWPHGLKTSCGPDVFSGSDDPGVLSYMIVLMITCCFIPLAVILLCYLQVWLAIRA VAAQQKESESTQKAEK----EVSRMVVVMIIAYCFCWGPYTVFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ FRNCIMQL   FGKKVDDGSELSSTS    RTEVSSVSNSSVSPA
>LWS_Lithoch   RYWPHGLKTSCGPDVFSGSEDPGVQSYMIVLMLTCCIFPLAIIILCYLAVWMAIRA VAMQQKESESTQKAER----EVSRMVVVMIVAYCVCWGPYTFFACFAAANPGYAFHPLAAAMPAYFAKSATIYNPIIYVFMNRQ FRTCIMQL   FGKQVDDGSEVSTS     KTEV     SSVAPA
>LWS_Gastero   RYWPHGLKTSCGPDVFSGSEDPGVQSYMIVLMITCCLIPLAIIILCYLAVWLAIRA VAMQQKESESTQKAER----DVSRMVVVMIVAYIVCWGPYTTFACFAAANPGYAFHPLAAAMPAYFAKSATIYNPVIYVFMNRQ FRSCIMQL   FGKEVDDGSEVSTs     KTEV     SSVAPA
>LWS_Petromy   RYWPHGLKTSCGPDVFSGSTDPGVQSYMVVLMITCCFLPLSIIILCYLQVWLAIHS VAQQQKESETTQKAER----DVSRMVVVMILAYVFCWGPYTFFACFAAANPGYSFHPIAAALPAYFAKGATIYNPIIYVFMNRQ FRNCILQL   FGKKVDDGSEVSSSS    RTEVSSVSNSSVSPA
>LWS_lamprey   RYWPHGLKTSCGPDVFSGSSDPGVQSYMVVLMVTCCFLPLSVIILCYLQVWLAIHS VAQQQKESETTQKAER----DVSRMVVVMILAYIFCWGPYTFFACYAAANPGYAFHPLTAALPAYFAKSATIYNPVIYVFMNRQ FRNCIMQL   FGKKVDDGSEVSSAS    RTEVSSVSNSSISPA
>LWS_Geotria   RYWPHGLKTSCGPDVFSGSTDPGVQSYMVVLMITCCFIPLALIIICYLQVWLAIHT VAQQQKESETTQKAER----DVSRMVVVMIFAYIFCWGPYTFFACFAAANPGYAFHPLAAALPAYFAKSATIYNPIIYVFMNRQ FRNCIMQL   FGKKVDDGSEVSSSA    RTEVSSVSNSSVSPA
>PIN_galGal    SYVPEGLRTSCGPNWYTGGSNNN--SYILSLFVTCFVLPLSLILFSYTNLLLTLRA AAAQQKEADTTQRAER----EVTRMVIVMVMAFLLCWLPYSTFALVVATHKGIIIQPVLASLPSYFSKTATVYNPIIYVFMNKQ FQSCLLEMLC CGYQPQRTGKASPGTPGPHADVTAAGLRNKVMPAHPV
>PIN_Uta sta   SYVPEGLRTSCGPNWYTGGSGNN--SYIMALFVTCFALPLGMIIFSYASLLLTLRA VATQQKEVETTQQAEK----EVTRRVIAMVMAFLVCWLPYASFAMVVATNKDLVIQPALASLPSYFSKTATVYNPIIYVFMNKQ FRSCLLSTMS CGHRPRGAQETTPAMISIPQGPTSALQGSRNKVTPSA
>PIN_Phelsuma  SYVPEGLGTSCGPNWYMGGTNNN--SYIVALFVTCFALPLSMILFSYANLLLTLRA VAAQQKEQETTQRAEK----EVTRMVITMVMAFLVCWLPYATFAMVVATTKDLSIQPGLASLPSYFSKTATVYNPIIYVFMNKQ FRSCLLNTVS CGRIPQTMPGTPATTAVRGGFVLTSEGRGNKVASTEL
>PIN_Podarcis  SYVPEGLRTSCGPNWYSGGSSNN--SYIMTLFVTCFAMPLSTILFSYANLLMTLRT VAAQQKEQETTQRAER----EVTRMVVAMVAAFLVCWLPYASFAMVVATHKDLAIRPALASLPSYFSKTATVYNPIIYVFMNKQ FRSCLLYKMS CGHRALSSQDTTPAGISLPGRLTTSASKGSRNQVSPS
>PIN_xenTro    SYVPEGLRTSCGPNWYTGGTNNN--SYIMALFLTCFIMPLSTIIFSYSNLLMALRA VAAQQKDSETTQRAEK----EVTRMVIAMVLAFLICWLPYASFAVVVAVNKDVVIEPTVASLPSYFSKTATVYNPIIYVFMNKQ FRNCLMTLLC CGRSFGDDETSSASGRTDVTSVSEAGGNKVTPA
>PIN_Bufo      SYVPEGLGTSCGPNWYTGGTNNN--SYILALFTTCFMMPLTTIIFSYSNLLLALRA VAAQQKESETTQRAER----EVTRMVIAMVLAFLICWLPYAVFAIVMASNKNVVIDPTLASMPSYFSKTATVYNPVIYVFMNKQ FRDCLTKLLC CGRNPFGEDETSTTSGRTDVTSVSEGGGNKVTPA
>CILL2_Platyn  SYRPEGLGTWCSISWQDRSMNTM--SYVTAVFLGCYFFPVSIIIFCYFNVWRKVKE AADAQGAGTAGKAEKS-----IFRMSVIMVTCYLTAWTPYAIVCLIASYGPPNGLPIYAEVLPSLFAKSSQVYNPIIYVLMNKP 
>ENCEPH_braBel SYQFEGHSVGCSVNWVKHNVNNV--SYIITLMVTCFFVPMVVVCWSYACIWRTVRM SAEMKSEFGNPQNTGR----LVTTMVVVMIVCFLVCWTPYTVMALIVTFGADHLVTPTASVIPSLVAKSSTAYNPIIYVLMNNQ FREFLLARLRTFCCRQPRMLRVTPMDDNAHARLVGEGPSHAQQVIPSEEN
>ENCEPH_braFlo SYQFEGHNVGCSVNWVQHNPDNV--SYIVTLMVTCFFVPMVVVCWSYAWIWRTVRM SSEAKPECGNSQNAGR----LVTTMVVVMIICFLVCWTPYAVMALIVTFGADHLVTPTASVIPSLVAKSSTAYNPIIYVLMNNQ FREFLLARLQRVCCRQQAVPRVTPMDDNVHVRLGGEGPSQSQQFLPAGEN
>VAOP_galGal   SYTTSKIGTTCEPNWYSGAYNDR--SYIIAFFTTCFIVPLLVILVSYGKLLQKLRK VSNTQGRLRTARKPER----QVTRMVVVMIIAFLICWMPYAVFSILATAYPSIELDPHLAAIPAFFSKTATVYNPIIYVFMNKQ FRMCLIQMFK CSAIETAESNMNPTSERATLTQDKRDSQLSVMAVRST
>VAOP_anoCar   SYTTSKIGTTCEPNWYSGDYNDH--TFIITFFTTCFILPLLVILVSYGKLMRKLRK VSDTQGRLGTTRKPER----QVTGMVVIMILAFLICWSPYAAFSILVTACPSIELDPRLAAIPAFFSKTATVYNPVIYVFMNNQ FRKCLVQLFQ CSSQETMDANVNPISEKDTLTHTKHCGEMSTVAAHVI
>VAOP_xenTro   SYTTSKIGTTCEPNWYSGEMRDH--TYIITFLTTCFVFPLLVIFMSYGKLMRKLRK VSDTQGRLGSTRKPEK----EVTRMVVIMILAFLICWTPYAAFSILITAHPTIDLDPRLAAIPAFFAKTASMYNPIIYVYMNKQ FRRCLYQMFN INDPEAKESNLNPTSERGVLTRNNNGGEMLAIATHIT
>VAOP_Danio    SYTVSRIGTTCEPNWYSGNFHDH--TFIITLFSTCFIFPLGVIIVCYCKLIRKLRK VSNTHGRLGNARKPER----QVTRMVVVMIVAFMVAWTPYAAFSIIITAHPSMHVDPRLAAIPAFVAKTAAVYNPIIYVFMNKQ FRKCLVQLLS CSKVTVVEGNNNQTTERAGMTSGSNTGEMSAIAARVS
>VAOP_Ruti     SYTVSKIGTTCEPNWYSGNFHDH--TFIIAFFITCFILPLGVIVVCYCKLIKKLRK VSNTHGRLGNARKPER----QVTRMVVVMIVAFMVAWTPYAAFSIVVTAHPSIHLDPRLAAAPAFFSKTAAVYNPVIYVFMNKQ FRKCLVQLLR CRDVTIIEGNINQTSERQGMTNESHTGEMSTIASRIP
>VAOP_Petrom   SYRPSMIGTTCEPNWYSGELHDH--TFILMFFSTCFIFPLAVIFFSYGKLIQKLKK ASETQRGLESTRRAEQ----QVTRMVVVMILAFLVCWMPYATFSIVVTACPTIHLDPLLAAVPAFFSKTATVYNPVIYIFMNKQ FRDCFVQVLP CKGLKKVSATQTAGAQDTEHTASVNTQSPGNRHNIAL
>PPIN_anaCar   GYQMEGVMTSCAPDWANSDPINV--SYIICYFLFCFTIPFITILASYGYLIWTLRQ VAKVGLAQRGSTTKAEA---QVSRMVIVMVMAFLICWLPYATFALVVVGNPQIYINPIIATIPMYMAKSSTFYNPIIYIFMNKQ FRDCLVRCLL CGRNPCASEQTDEDDLEVSTIAPAPSSRRGKVAPV
>PPIN_Xeno     SYELEGVMTSCAPNWYSADPVNM--SYIVCYFSFCFAIPFLIIVGSYGYLMWTLRQ VAKLGVAEGGTTSKAEV---QVSRMVIVMILAFLVCWLPYAAFAMTVVANPGMHIDPIIATVPMYLTKTSTVYNPIIYIFMNKQ FQECVIPFLF CGRNPWAAEKSSSMETSISVTSGTPTKRGQVAPA
>PPIN_Icta     SYQLEGVMTSCAPNWYRRDPVNV--SYILCYFMLCFALPFATIIFSYMHLLHTLWQ VAKLQVADSGSTAKVEV---QVARMVVIMVMAFLLTWLPYAAFALTVIIDSNIYINPVIGTIPAYLAKSSTVFNPIIYIFMNRQ FRDYALPCLL CGKNPWAAKEGRDSDTNTLTTTVSKNTSVSPL
>PPIN_Danio    RLQLEGVRTSCAPDWYSRDLANV--SFIVCYFLLCFALPFSVIVYSYTRLLWTLRQ VSRLQVCEGGSAARAEA---QVSCMVVVMILAFLLTWLPYASFALCVILIPELYIDPVIATVPMYLTKSSTVFNPIIYIFMNRQ FRDRALPFLL CGRNPWAAEAEEEEEETTVSSVSRSTSVSPA
>PPIN_Oncorhy  SFELEGVRTSCSPNWYSREPGNM--SYIILYFLLCFAIPFSIIMVSYARILFTLHQ VSKLKVLEGNSTTRVEI---QVVRMVVVMVMAFLLSWLPYAAFALSVILDPSLHINPLIATVPMYLAKSSTVYNPIIYVFMNRQ FRDCAVPFLL CGLNPWASEPVGSEADTALSSVSKNPRVSPQs
>PPIN_lamp     SYELEGVRTSCAPDWYSRDPANV--SYITSYFAFCFAIPFLVIVVAYGRLMWTLHQ VAKLGMGESGSTAKAEA---QVSRMVVVMVVAFLVCWLPYALFAMIVVTKPDVYIDPVIATLPMYLTKTSTVYNPIIYIFMNRQ FRDCAVPFLL CGRNPWAEPSSESATAASTSATSVTLASAPGQVSPS
>PARIE_Uta     SYGPEGVQTSCSIGWEERSWSNY--SYLIVYFLSCFFIPVLIIGFSYGNVIRSLHG LNKKVEQLGGKSSPEEEF--RAVIMVLVMVVAFLICWLPYTVFALIVVFNPALNISPLAATIPTYLSKTSPVYNPIIYIFLNKQ FRDCAVEFIT CGQVVLTSPEEDISTSAIPVEGKGPCKINQVTPV
>PARIE_Anole   SYGPEGVQTSCSIGWEERSWNNY--SYLIVYFLSCFFIPVLIIGFSYGNVIRSLHG LNKKVEQLGGKSNPEEEF--RAVIMVLVMVVAFLICWLPYTLFALTVVFNPALNISPLAATIPTYLSKTSPVYNPIIYIFLNKE FRECAVEFIT CGKVVLTSPEEDISTSAISDEGIAPCKINQVTPV
>PARIE_Xenop   SYGPEGVQTSCSIGWEERSWSNY--SYIISYFLTCFIIPVGIIGFSYGSILRSLHQ LNRKIEQQGGKTNPREEK--RVVIMVLFMVLAFLICWLPYTVFALIVVINPQLYISPLAATLPTYFAKTSPVYNPIIYIFLNKQ FRTYAVQCLT CGHINLDSLEEDTESVSAQAENMLTPKTNQVAPA
>ENCEPH_braBel SYQLEGPKIGCSVAWEEHSWSNT--SYIVVLFITCLFAPLLIIVYSYYRLWHKVKQ GSRNLPAAMRKSSQKEQ---KIAMMVIVMITCFMVCWLPYGAMALVVTFGGERLISHTAAVVPSLLAKSSTCYNPVVYFAMNSQ FRRYFQDLLC CGRRLFDVSQSVVTGNTAMPRNNSQGFRKDDSDQKQD
>ENCEPH_homSap RYILDVHGLGCTVDWKSKDANDS--SFVLFLFLGCLVVPLGVIAHCYGHILYSIRM LRCVEDLQTIQVIKILKYEKKLAKMCFLMIFTFLVCWMPYIVICFLVVNGHGHLVTPTISIVSYLFAKSNTVYNPVIYVFMIRK FRRCLLQLLC FRLLKFQQPKKDRPVIRTEKQIRPIVMSQKVGDRPKKKVT
>ENCEPH_monDom RYTLEIHGLGCSVDWKSKDPNDS--SFVIFLFFGCLMLPVGVMAYCYGHILYAIRM LRCVEELQTIQVIKILRYEKKVAKMCFLMIAIFLFCWMPYAVICLLVANGYGSLVTPTVAIIASLFAKSSTAYNPIIYIFMSRK FRRCLLQLLC FRLLKFQQPKKDRPVIRTEKQIRPIVMSQKVGDRPKKKVT
>ENCEPH_galGal RYTLEIHGLGCSMDWKSKDPNDT--SFVLLFFLGCLVAPVVIMAYCYGHILYAVRM LRCVEDFQTSQVIKLLKYEKKVAKMCFLMISTFLICWMPYAVVSLLVTYGYSNLVTPTVAIIPSFFAKSSTAYNPVIYIFMSRK FRQCLLQLLC FRLMRFQRIMKEPSGAGNVKPIRPIVMSQKVGDRPKKKVT
>ENCEPH_anoCar HYTLEIHGLGCSVDWQSKEPSDS--SFVLFFFLGCLAAPVGIMAYCYGHILHAIRM LRCVEDLQSIQVIKILRYEKKVAKMCFLMVTTFLICWMPYAVVSLLIAYGYGHLITPTVAIIPSFFAKSSTAYNPVIYIFMSRK FRRCLVQLFC VQFLRFKRTLKEQPAIESNKPIRPIVMSQKVGDRPKKKVT
>ENCEPH_xenTro LYTFETHKLDCSFEWTATDPKDT--AFVLLFFLACITLPLSIMAYCYGYILYEIQK LRSVKNIQNFQEITILDYEIKMAKMCLLMMLTFLIGWMPYTILSLLVTSGYSKFITPTITVMPSLLAIASAAYNPVIHIFTIKK FRQCLVQLLP PINFHPPINPPINNFWRLLKNLNGRLAMKKVKPVLGKGRS
>ENCEPH1_Anoph AYVNEAANISCSVNWESQTANAT--SYIIFLFIFGLILPLAVIIYSYINIVLEMRK NSARVGRVNRAERRVT-------SMVAVMIVAFMVAWTPYAIFALIEQFGPPELIGPGLAVLPALVAKSSICYNPIIYVGMNTQ FRAAFWRIRR SNGVAGQPDSNNTNNSNRDKESARHTAKEGL
>ENCEPH2_Anoph AYVQEAANISCSVNWESQTKNAT--TYIIFLFVFGLVVPLIVIVYSYTNIIVNMRE NSARVGRINRAEQRVT-------SMVAVMIVAFMVAWTPYAIFALIEQFGPPELIGPGLAVLPALVAKSSICYNPIIYVGMNTQ FRAAFSRVRN KGQQAAADQNTTTMQRELTKSSRDMVECSF
>ENCEPH2_Apis  SYGPEAGNVSCSVSWEVHDPVTNSDTYIGFLFVLGLIVPVFTIVSSYAAIVLTLKK VRKRAGASGRREAKIT-------KMVALMITAFLLAWSPYAALAIAAQYFNAKPSATV-AVLPALLAKSSICYNPIIYAGLNNQ FSRFLKKIFD ARGSRTAVPDSQHTALTALNRQEQRK
>CILL1_Platyn  HYIPEGLATWCSIDWLSDETSDK--SYVFAIFIFCFLVPVLIIVVSYGLIYDKVRK VAKTGGSVAKAEREVL-------RMTLLMVSLFMLAWSPYAVICMLASFGPKDLLHPVATVIPAMFAKSSTMYNPLIYVFMNKQ FRRSLKVLLG MGVEDLNSESERATGGTATNQVAAT
>PER_homSap    SYAPDPTGATCTINWRKNDRSFV--SYTMTVIAINFIVPLTVMFYCYYHVTLSIKH HTTSDCTESLNRDWSD--QIDVTKMSVIMICMFLVAWSPYSIVCLWASFGDPKKIPPPMAIIAPLFAKSSTFYNPCIYVVANKK FRRAMLAMFK CQTHQTMPVTSILPMDVSQNPLASGRI
>PERa_Bran     EYALEPSGTACTINFQKNDSLYI--SYVTSCFVLGFVVPLAVMAFCYWQASCFVSK VLKGDIAGDLTFPVAAN.QNHFSKMCLAMVAAFVVAWTPYSVLFLFAAFWNPADIPAWLTLLPPLIAKSSALYNPIIYIIANRR FRNAICSMMK GQDPDVEDDEHADEHRVRSIEDNDKEIISMVNLNMTV
>PERc_Bran     YTYETPMQITCSLDWNVQHPGEK--AYIAAVLVIVYVLQVLIMCFCYFNIIFKSAN LKFAALASEKTKMAAKKDTWKTSVMCLTMVVSFLIAWTPYAVSSTWDILSAE-DLPIIATILPSLFAKSSCMMNPIIYACCNTK FRQAAVKSFR KLCGMCKQKVPLSTPQVVLAMQRNTEFTSTVEPT
>NEUR_homSap   DYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKS SSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYK FACCQTGGLK ATKKKSLEGFRLHTVTTVRKSSAVLEIHEEWE
>MEL_Platy     AYIPEGFQTSCTYDYLTQDMNNY--TYVLGMYLFGFIFPVAIIFFCYLGIVRAIFA HHAEMMATAKRMGAN...EIQIAKVAAMTIGTFMLSWTPYAVVGVFGMIKPHSEMFIH.AEIPVMMAKASARYNPIIYALSHPK FRAEIDKHFPWLLCCCKPKPKAQLPSSTTKGSIASKTEADTSV
>MEL1_homSap   AYVPEGLLTSCSWDYMSFTPAVR--AYTMLLCCFVFFLPLLIIIYCYIFIFRAIRE TGRALQTFGACKGNG.QSECKMAKIMLLVILLFVLSWAPYSAVALVAFAGYAHVLTPYMSSVPAVIAKASAIHNPIIYAITHPK YRVAIAQHLP CLGVLLGVSRRHSRPYPSYRSTHRSTLTSHTSNL

>PPINa_Ciona   YDTEGLGTSCAPNWFVKEKRERL--FIILYFVFCFVIPLAVIMICYGKLILTLRQ IAKESSLSGGTSPEGEVTKMVVVMVTAFVFCWLPYAAFAMYNVVNPEAQ IDYALGAAPAFFAKTATIYNPLIYIGLNRQ FRDCVVRMIF NGRNPWVDELVGSQVSSTGSQLTAVSSNKVAPA
>PPINb_Ciona   GYVPEGLGTSCAPNWFSKNKSER--IFIFVYFVFCFFIPLLVIIICYGKIVLFLKQVSLY ATRQSSASSNRQADNKVTKMVLVMISAFLICWTPYGVLSLYNAINPDKQ LDYGLGAVPVFFAKTANIYNPLIYIGLNKQ FRDGVIKMVF RGRNPWAEEMSTQQRQRSTEAGQPIVSNEV
>PPIN2_Ciona   SVIWHTPGLFFWNGYEPEGFGTS--CAPNWFSQQKRERIFIFAYFAFCFLTPLTIIFACYLKLILFIRKVSVSKKSMVNEADRRDFEVTRMVFVMIAAFLICWLPYGCLSMYNAIHPD FRDGVIRMLF KGRNPWLDGRNTTSSTSTRAQ


Structural and functional markers along the opsin molecule:

>RHO1_homSap rhodopsin                 <----------TM1--------->    c1     <----------TM2------->    x1      <c--i------TM3--------->           c2       <----------TM4-------->               x2         <----------TM5------c->     c3
MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKE 
AAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA* 0
           c3       <----------TM6------->    x3      <--------b-TM7---gprot>  helix8    palm cyto tail

Opsin evolution: Difference between revisions

Revision as of 21:33, 17 November 2007

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 The table below is a tab-delimited database that can be pasted into excel for further analysis. For example, sorting on the second column would show all the opsin sequences available for a fixed species. Sorting on the lambda max absorption column would group the data by color property (when that column is better filled in). The columns show gene name, genus, species, common name, heterotrimeric G protein alpha subunit used in signaling, intron structure, synteny (2 flanking genes on each side of the opsin), indel status, sequence length, lambda max, and comment field.
 <pre>
->RHO1_homSap	Homo	sapiens	(human)	Gt	0...2.1.0.0	-MBD4	+IFT122	+H1FOO	-PLXND1	indel	349 aa	497 nm		rod_rhodopsin	RHO
+	>RHO1_homSap	Homo	sapiens	(human)	Gt	0...2.1.0.0	-MBD4	+IFT122	+H1FOO	-PLXND1	indel	349 aa	497 nm
->RHO1_monDom	Monodelphis	domesticus	(opossum)	Gt	0...2.1.0.0	-MBD4	+IFT122	+H1FOO	-PLXND1	indel	349 aa	000 nm		rod_rhodopsin
+	>RHO1_monDom	Monodelphis	domesticus	(opossum)	Gt	0...2.1.0.0	-MBD4	+IFT122	+H1FOO	-PLXND1	indel	349 aa	000 nm
->RHO1_ornAna	Ornithorhynchus	anatinus	(platypus)	Gt	0...2.1.0.0	-	+IFT122	-	-PLXND1	indel	354 aa	000 nm		rod_rhodopsin
+	>RHO1_ornAna	Ornithorhynchus	anatinus	(platypus)	Gt	0...2.1.0.0	-	+IFT122	-	-PLXND1	indel	354 aa	000 nm
->RHO1_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	-MBD4	+IFT122	+H1FOO	-PLXND1	indel	352 aa	000 nm		rod_rhodopsin	RH1
+	>RHO1_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	-MBD4	+IFT122	+H1FOO	-PLXND1	indel	352 aa	000 nm
->RHO1_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2.1.0.0	-MBD4	+IFT122	-	-PLXND1	indel	343 aa	000 nm		rod_rhodopsin
+	>RHO1_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2.1.0.0	-MBD4	+IFT122	-	-PLXND1	indel	343 aa	000 nm
->RHO1_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.1.0.0	-MBD4	+IFT122	-	-PLXND1	indel	355 aa	000 nm		rod_rhodopsin
+	>RHO1_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.1.0.0	-MBD4	+IFT122	-	-PLXND1	indel	355 aa	000 nm
->RHO1_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	000 nm	17961206	rod_rhodopsin
+	>RHO1_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	000 nm	17961206
->RHO1_latCha	Latimeria	chalumnae	(coelacanth)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	478 nm	10339578	rod_rhodopsin
+	>RHO1_latCha	Latimeria	chalumnae	(coelacanth)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	478 nm	10339578
->RHO1_takRub	Takifugu	rubripes	(pufferfish)	Gt	0...2.1.0.0	-MBD4	+IFT122	-	-PLXND1	indel		000 nm		rod_rhodopsin
+	>RHO1_takRub	Takifugu	rubripes	(pufferfish)	Gt	0...2.1.0.0	-MBD4	+IFT122	-	-PLXND1	indel		000 nm
->RHO1_leuEri	Leucoraja	erinacea	(skate)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	000 nm		rod_rhodopsin
+	>RHO1_leuEri	Leucoraja	erinacea	(skate)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	000 nm
->RHO1_calMil	Callorhinchus	milii	(elephantfish)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	000 nm		rod_rhodopsin
+	>RHO1_calMil	Callorhinchus	milii	(elephantfish)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	000 nm
->RHO1_petMar	Petromyzon	marinus	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	354 aa	000 nm		rod_rhodopsin
+	>RHO1_petMar	Petromyzon	marinus	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	354 aa	000 nm
->RHO1_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	354 aa	497 nm	17463225	rod_rhodopsin
+	>RHO1_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	354 aa	497 nm	17463225
->RHO1_letJap	Lethenteron	japonicum	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	354 aa	000 nm		cone_rhodopsin
+	>RHO1_letJap	Lethenteron	japonicum	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	354 aa	000 nm
->RHO2_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	-IHPK3	-LEMD2	-GRM4	+HMGA1	indel	356 aa	000 nm		cone_rhodopsin
+	>RHO2_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	-IHPK3	-LEMD2	-GRM4	+HMGA1	indel	356 aa	000 nm
->RHO2_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2.1.0.0	-IHPK3	-LEMD2	-GRM4	+HMGA1	indel	356 aa	000 nm		cone_rhodopsin
+	>RHO2_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2.1.0.0	-IHPK3	-LEMD2	-GRM4	+HMGA1	indel	356 aa	000 nm
->RHO2_gekGek	Gekko	gekko	(gecko)	Gt	0...2.1.0.0	x	x	x	x	indel	356 aa	000 nm		cone_rhodopsin
+	>RHO2_gekGek	Gekko	gekko	(gecko)	Gt	0...2.1.0.0	x	x	x	x	indel	356 aa	000 nm
->RHO2_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	356 aa	000 nm	17961206	cone_rhodopsin
+	>RHO2_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	356 aa	000 nm	17961206
->RHO2_latCha	Latimeria	chalumnae	(coelacanth)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	485 nm	10339578	cone_rhodopsin	RH2
+	>RHO2_latCha	Latimeria	chalumnae	(coelacanth)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	485 nm	10339578
->RHO2_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	492 nm	17463225	cone_rhodopsin	RhB
+	>RHO2_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	355 aa	492 nm	17463225
->SWS2_ornAna	Ornithorhynchus	anatinus	(platypus)	Gt	0...2.1.0.0	-IRAK1	-MECP2	-	+TKTL1	indel	364 aa	000 nm		cone_short2
+	>SWS2_ornAna	Ornithorhynchus	anatinus	(platypus)	Gt	0...2.1.0.0	-IRAK1	-MECP2	-	+TKTL1	indel	364 aa	000 nm
->SWS2_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	x	x	x	x	indel	362 aa	000 nm		cone_short2
+	>SWS2_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	x	x	x	x	indel	362 aa	000 nm
->SWS2_taeGut	Taeniopygia	guttata	(finch)	Gt	0...2.1.0.0	x	x	x	x	indel	363 aa	000 nm		cone_short2
+	>SWS2_taeGut	Taeniopygia	guttata	(finch)	Gt	0...2.1.0.0	x	x	x	x	indel	363 aa	000 nm
->SWS2_utaSta	Uta	stansburiana	(lizard)	Gt	0...2.1.0.0	x	x	x	x	indel	364 aa	000 nm		cone_short2
+	>SWS2_utaSta	Uta	stansburiana	(lizard)	Gt	0...2.1.0.0	x	x	x	x	indel	364 aa	000 nm
->SWS2_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.1.0.0	-IRAK1	-MECP2	-	-	indel	363 aa	000 nm		cone_short2
+	>SWS2_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.1.0.0	-IRAK1	-MECP2	-	-	indel	363 aa	000 nm
->SWS2_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	364 aa	000 nm	17961206	cone_short2
+	>SWS2_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	364 aa	000 nm	17961206
->SWS2_takRub	Takifugu	rubripes	(pufferfish)	Gt	0...2.1.0.0	x	x	x	x	indel	351 aa	000 nm	genome	cone_short2
+	>SWS2_takRub	Takifugu	rubripes	(pufferfish)	Gt	0...2.1.0.0	x	x	x	x	indel	351 aa	000 nm	genome
->SWS2_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gt	0.2.2.1.0.0	x	x	x	x	indel	359 aa	000 nm	genome	cone_short2
+	>SWS2_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gt	0.2.2.1.0.0	x	x	x	x	indel	359 aa	000 nm	genome
->SWS2_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	362 aa	439 nm	17463225	cone_short2
+	>SWS2_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	362 aa	439 nm	17463225
->SWS1_homSap	Homo	sapiens	(human)	Gt	0.2.2.1.0.0	-FAM137A	-CALU	-NAG6	-FLNC	indel	348 aa	000 nm		cone_short1
+	>SWS1_homSap	Homo	sapiens	(human)	Gt	0.2.2.1.0.0	-FAM137A	-CALU	-NAG6	-FLNC	indel	348 aa	000 nm
->SWS1_monDom	Monodelphis	domesticus	(opossum)	Gt	0...2.1.0.0	-FAM137A	-CALU	-NAG6	-FLNC	indel	347 aa	000 nm		cone_short1
+	>SWS1_monDom	Monodelphis	domesticus	(opossum)	Gt	0...2.1.0.0	-FAM137A	-CALU	-NAG6	-FLNC	indel	347 aa	000 nm
->SWS1_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	x	x	x	x	indel	348 aa	000 nm		cone_short1
+	>SWS1_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	x	x	x	x	indel	348 aa	000 nm
->SWS1_taeGut	Taeniopygia	guttata	(finch)	Gt	0...2.1.0.0	x	x	x	x	indel	347 aa	000 nm		cone_short1
+	>SWS1_taeGut	Taeniopygia	guttata	(finch)	Gt	0...2.1.0.0	x	x	x	x	indel	347 aa	000 nm
->SWS1_anoCar	Anolis	carolinensis	(lizard)	Gt	0.2.2.1.0.0	x	-CALU	x	x	indel	347 aa	000 nm	genome	cone_short1
+	>SWS1_anoCar	Anolis	carolinensis	(lizard)	Gt	0.2.2.1.0.0	x	-CALU	x	x	indel	347 aa	000 nm	genome
->SWS1_utaSta	Uta	stansburiana	(lizard)	Gt	0...2.1.0.0	x	x	x	x	indel	348 aa	000 nm		cone_short1
+	>SWS1_utaSta	Uta	stansburiana	(lizard)	Gt	0...2.1.0.0	x	x	x	x	indel	348 aa	000 nm
->SWS1_xenLae	Xenopus	laevis	(frog)	Gt	0...2.1.0.0	-	-CALU	-	-	indel	348 aa	000 nm		cone_short1
+	>SWS1_xenLae	Xenopus	laevis	(frog)	Gt	0...2.1.0.0	-	-CALU	-	-	indel	348 aa	000 nm
->SWS1_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	347 aa	000 nm	17961206	cone_short1
+	>SWS1_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0...2.1.0.0	x	x	x	x	indel	347 aa	000 nm	17961206
->SWS1_danRer	Danio	rerio	(zebrafish)	Gt	0...2.1.0.0	-	-CALU	-	-	indel	337 aa	000 nm		cone_short1
+	>SWS1_danRer	Danio	rerio	(zebrafish)	Gt	0...2.1.0.0	-	-CALU	-	-	indel	337 aa	000 nm
->SWS1_oryLat	Oryzias	latipes	(medaka)	Gt	0...2.1.0.0	-	-	-	-	indel	336 aa	000 nm		cone_short1
+	>SWS1_oryLat	Oryzias	latipes	(medaka)	Gt	0...2.1.0.0	-	-	-	-	indel	336 aa	000 nm
->SWS1_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	346 aa	359 nm	17463225	cone_short1
+	>SWS1_geoAus	Geotria	australis	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	346 aa	359 nm	17463225
->LWS_homSap	Homo	sapiens	(human)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-TEX28	+TKTL1	indel	364 aa	530 nm		cone_long	OPN1MW
+	>LWS_homSap	Homo	sapiens	(human)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-TEX28	+TKTL1	indel	364 aa	530 nm
->LWS_monDom	Monodelphis	domesticus	(opossum)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-	+TKTL1	indel	368 aa	000 nm		cone_long
+	>LWS_monDom	Monodelphis	domesticus	(opossum)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-	+TKTL1	indel	368 aa	000 nm
->LWS_ornAna	Ornithorhynchus	anatinus	(platypus)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-	-	indel	365 aa	000 nm		cone_long
+	>LWS_ornAna	Ornithorhynchus	anatinus	(platypus)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-	-	indel	365 aa	000 nm
->LWS_galGal	Gallus	gallus	(chicken)	Gt	0.2.2.1.0.0	x	x	x	x	indel	363 aa	000 nm		cone_long
+	>LWS_galGal	Gallus	gallus	(chicken)	Gt	0.2.2.1.0.0	x	x	x	x	indel	363 aa	000 nm
->LWS_anoCar	Anolis	carolinensis	(lizard)	Gt	0.2.2.1.0.0	-	-	-TEX28	+TKTL1	indel	370 aa	000 nm		cone_long
+	>LWS_anoCar	Anolis	carolinensis	(lizard)	Gt	0.2.2.1.0.0	-	-	-TEX28	+TKTL1	indel	370 aa	000 nm
->LWS_xenTro	Xenopus	tropicalis	(frog)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-	-	indel		000 nm		cone_long
+	>LWS_xenTro	Xenopus	tropicalis	(frog)	Gt	0.2.2.1.0.0	-IRAK1	-MECP2	-	-	indel		000 nm
->LWS_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0.2.2.1.0.0	x	x	x	x	indel	365 aa	000 nm	17961206	cone_long
+	>LWS_neoFor	Neoceratodus	forsteri	(lungfish)	Gt	0.2.2.1.0.0	x	x	x	x	indel	365 aa	000 nm	17961206
->LWS_takRub	Takifugu	rubripes	(pufferfish)	Gt	0...2.1.0.0	x	x	x	x	indel	358 aa	000 nm	genome
+	>LWS_takRub	Takifugu	rubripes	(pufferfish)	Gt	0...2.1.0.0	x	x	x	x	indel	358 aa	000 nm	genome
->LWS_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gt	0.2.2.1.0.0	-	-	-	-	indel	358 aa	000 nm	genome	cone_long
+	>LWS_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gt	0.2.2.1.0.0	-	-	-	-	indel	358 aa	000 nm	genome
->LWS_petMar	Petromyzon	maritimus	(lamprey)	Gt	0.2.2.1.0.0	x	x	x	x	indel	366 aa	000 nm		cone_long
+	>LWS_petMar	Petromyzon	maritimus	(lamprey)	Gt	0.2.2.1.0.0	x	x	x	x	indel	366 aa	000 nm
->LWS_letJap	Lethenteron	japonicum	(lamprey)	Gt	0.2.2.1.0.0	x	x	x	x	indel	365 aa	000 nm		cone_long
+	>LWS_letJap	Lethenteron	japonicum	(lamprey)	Gt	0.2.2.1.0.0	x	x	x	x	indel	365 aa	000 nm
->LWS_geoAus	Geotria	australis	(lamprey)	Gt	0.2.2.1.0.0	x	x	x	x	indel	365 aa	560 nm	17463225	cone_long
+	>LWS_geoAus	Geotria	australis	(lamprey)	Gt	0.2.2.1.0.0	x	x	x	x	indel	365 aa	560 nm	17463225
->PIN_galGal	Gallus	gallus	(chicken)	Gt	0...2.2.0.0					indel	352 aa	000 nm		pinopsin
+	>PIN_galGal	Gallus	gallus	(chicken)	Gt	0...2.2.0.0					indel	352 aa	000 nm
->PIN_utaSta	Uta	stansburiana	(lizard)	Gt	0...2.2.0.0	x	x	x	x	indel	359 aa	000 nm		pinopsin
+	>PIN_utaSta	Uta	stansburiana	(lizard)	Gt	0...2.2.0.0	x	x	x	x	indel	359 aa	000 nm
->PIN_pheMad	Phelsuma	madagascariensis	(gecko)	Gt	0...2.2.0.0	x	x	x	x	indel	358 aa	000 nm		pinopsin
+	>PIN_pheMad	Phelsuma	madagascariensis	(gecko)	Gt	0...2.2.0.0	x	x	x	x	indel	358 aa	000 nm
->PIN_podSic	Podarcis	sicula	(lizard)	Gt	0...2.2.0.0	x	x	x	x	indel	354 aa	000 nm		pinopsin
+	>PIN_podSic	Podarcis	sicula	(lizard)	Gt	0...2.2.0.0	x	x	x	x	indel	354 aa	000 nm
->PIN_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.2.0.0					indel	346 aa	000 nm		pinopsin
+	>PIN_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.2.0.0					indel	346 aa	000 nm
->PIN_bufJap	Bufo	japonicus	(frog)	Gt	0...2.2.0.0	x	x	x	x	indel	347 aa	000 nm		pinopsin
+	>PIN_bufJap	Bufo	japonicus	(frog)	Gt	0...2.2.0.0	x	x	x	x	indel	347 aa	000 nm
->VAOP_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	+INPP5A	-NXK6	+C10orf61	+ALDH18A1	indel	393 aa	000 nm		vertebrate_ancient
+	>VAOP_galGal	Gallus	gallus	(chicken)	Gt	0...2.1.0.0	+INPP5A	-NXK6	+C10orf61	+ALDH18A1	indel	393 aa	000 nm
->VAOP_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2.1.0.0	+INPP5A	-NXK6	+GPR125	+KNDC1	indel	389 aa	000 nm		vertebrate_ancient
+	>VAOP_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2.1.0.0	+INPP5A	-NXK6	+GPR125	+KNDC1	indel	389 aa	000 nm
->VAOP_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.1.0.0	-	+GSTO2	-C10orf92	-	indel	383 aa	000 nm		vertebrate_ancient
+	>VAOP_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2.1.0.0	-	+GSTO2	-C10orf92	-	indel	383 aa	000 nm
->VAOP_danRer	Danio	rerio	(zebrafish)	Gt	0...2.1.0.0	-	-	-	-	indel	378 aa	000 nm		vertebrate_ancient
+	>VAOP_danRer	Danio	rerio	(zebrafish)	Gt	0...2.1.0.0	-	-	-	-	indel	378 aa	000 nm
->VAOP_takRub	Takifugu	rubripes	(teleost)	Gt	0...2.1.0.0	+INPP5A	-NXK6	-	+KNDC1	indel	362 aa	000 nm		vertebrate_ancient
+	>VAOP_takRub	Takifugu	rubripes	(teleost)	Gt	0...2.1.0.0	+INPP5A	-NXK6	-	+KNDC1	indel	362 aa	000 nm
->VAOP_rutRut	Rutilus	rutilus	(minnow)	Gt	0...2.1.0.0	x	x	x	x	indel	383 aa	000 nm		vertebrate_ancient
+	>VAOP_rutRut	Rutilus	rutilus	(minnow)	Gt	0...2.1.0.0	x	x	x	x	indel	383 aa	000 nm
->VAOP_petMar	Petromyzon	marinus	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	445 aa	000 nm	9427550	vertebrate_ancient
+	>VAOP_petMar	Petromyzon	marinus	(lamprey)	Gt	0...2.1.0.0	x	x	x	x	indel	445 aa	000 nm	9427550
->PPIN_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2...0.0	-CPEB2	-CACNA2D3	+SELK	+ACTR8	indel	346 aa	000 nm		parapinopsin
+	>PPIN_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2...0.0	-CPEB2	-CACNA2D3	+SELK	+ACTR8	indel	346 aa	000 nm
->PPIN_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2...0.0	-	-	+SELK	-	indel	349 aa	000 nm		parapinopsin
+	>PPIN_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2...0.0	-	-	+SELK	-	indel	349 aa	000 nm
->PPIN_ictPun	Ictalurus	punctatus	(catfish)	Gt	0...2...0.0	x	x	x	x	indel	347 aa	000 nm		parapinopsin
+	>PPIN_ictPun	Ictalurus	punctatus	(catfish)	Gt	0...2...0.0	x	x	x	x	indel	347 aa	000 nm
->PPIN_danRer	Danio	rerio	(zebrafish)	Gt	0...2...0.0	-	-	+SELK	-	indel	338 aa	000 nm		parapinopsin
+	>PPIN_danRer	Danio	rerio	(zebrafish)	Gt	0...2...0.0	-	-	+SELK	-	indel	338 aa	000 nm
->PPIN_oncMyk	Oncorhynchus	mykiss	(trout)	Gt	0...2...0.0	x	x	x	x	indel	347 aa	000 nm		parapinopsin
+	>PPIN_oncMyk	Oncorhynchus	mykiss	(trout)	Gt	0...2...0.0	x	x	x	x	indel	347 aa	000 nm
->PPIN_petMar	Petromyzon	maritimus	(lamprey)	Gt	0...2...0.0	x	x	x	x	indel	344 aa	000 nm		parapinopsin
+	>PPIN_petMar	Petromyzon	maritimus	(lamprey)	Gt	0...2...0.0	x	x	x	x	indel	344 aa	000 nm
->PPIN_letJap	Lethenteron	japonicum	lamprey	Gt	0...2...0.0	x	x	x	x	indel	344 aa	000 nm		parapinopsin
+	>PPIN_letJap	Lethenteron	japonicum	lamprey	Gt	0...2...0.0	x	x	x	x	indel	344 aa	000 nm
->PPINa_cioInt	Ciona	intestinalis	(tunicate)	Gt	0...2...0.0	-HOXB1	+HHEX	+CUL4A	-	indel	391 aa	000 nm	11591373	parapinopsin
+	>PPINa_cioInt	Ciona	intestinalis	(tunicate)	Gt	0...2...0.0	-HOXB1	+HHEX	+CUL4A	-	indel	391 aa	000 nm	11591373
->PPINb_cioInt	Ciona	intestinalis	(tunicate)	Gt	0...2...0.0	-TMEM165	+FUT4	-	-	indel	353 aa	000 nm		parapinopsin
+	>PPINb_cioInt	Ciona	intestinalis	(tunicate)	Gt	0...2...0.0	-TMEM165	+FUT4	-	-	indel	353 aa	000 nm
->PARIE_utaSta	Uta	stansburiana	(lizard)	Gd+Go	0...2...0.0	x	x	x	x	indel	347 aa	522 nm	16543463	parietopsin
+	>PARIE_utaSta	Uta	stansburiana	(lizard)	Gd+Go	0...2...0.0	x	x	x	x	indel	347 aa	522 nm	16543463
->PARIE_anoCar	Anolis	carolinensis	(lizard)	Gd+Go	0...2...0.0	+EEA1	-FLJ46688	+BTG1	-	indel	347 aa	000 nm	16543463	parietopsin
+	>PARIE_anoCar	Anolis	carolinensis	(lizard)	Gd+Go	0...2...0.0	+EEA1	-FLJ46688	+BTG1	-	indel	347 aa	000 nm	16543463
->PARIE_xenTro	Xenopus	tropicalis	(frog)	Gd+Go	0...2...0.0	-lum	-DCN	-	-	indel	346 aa	000 nm	16543463	parietopsin
+	>PARIE_xenTro	Xenopus	tropicalis	(frog)	Gd+Go	0...2...0.0	-lum	-DCN	-	-	indel	346 aa	000 nm	16543463
->PARIE_takRub	Takifugu	rubripes	(teleost)	Gd+Go	0...2...0.0	-HSP90B1	+NT5DC2	-KCND3	-FLNC	indel	 351 aa	000 nm	16543463	parietopsin
+	>PARIE_takRub	Takifugu	rubripes	(teleost)	Gd+Go	0...2...0.0	-HSP90B1	+NT5DC2	-KCND3	-FLNC	indel	 351 aa	000 nm	16543463
->PARIE_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gd+Go	0...2...0.0	-HSP90B1	+NT5DC2	-KCND3	-FLNC	indel	361 aa	000 nm	-	parietopsin
+	>PARIE_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gd+Go	0...2...0.0	-HSP90B1	+NT5DC2	-KCND3	-FLNC	indel	361 aa	000 nm	-
->PARIE_danRer	Danio	rerio	(zebrafish)	Gd+Go	0...2...0.0	-	+NT5DC2	+FBXL13	-	indel	337 aa	000 nm	16543463	parietopsin
+	>PARIE_danRer	Danio	rerio	(zebrafish)	Gd+Go	0...2...0.0	-	+NT5DC2	+FBXL13	-	indel	337 aa	000 nm	16543463
->ENCEPH_homSap	Homo	sapiens	(human)	Gt	0...2...0.0	-EXO1	-WDR64	-KMO	+FH	indel	403 aa	000 nm		parietopsin	OPN3
+	>ENCEPH_homSap	Homo	sapiens	(human)	Gt	0...2...0.0	-EXO1	-WDR64	-KMO	+FH	indel	403 aa	000 nm
->ENCEPH_monDom	Monodelphis	domestica	(opossum)	Gt	0...2...0.0	-EXO1	-WDR64	-KMO	+FH	indel	411 aa	000 nm		encephalopsin	OPN3
+	>ENCEPH_monDom	Monodelphis	domestica	(opossum)	Gt	0...2...0.0	-EXO1	-WDR64	-KMO	+FH	indel	411 aa	000 nm
->ENCEPH_galGal	Gallus	gallus	(chicken)	Gt	0...2...0.0	-EXO1	-WDR64	-PIGM	+RGS7	indel	396 aa	000 nm		encephalopsin	OPN3
+	>ENCEPH_galGal	Gallus	gallus	(chicken)	Gt	0...2...0.0	-EXO1	-WDR64	-PIGM	+RGS7	indel	396 aa	000 nm
->ENCEPH_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2...0.0	-EXO1	-WDR64	-PIGM	+RGS7	indel	408 aa	000 nm		encephalopsin	OPN3
+	>ENCEPH_anoCar	Anolis	carolinensis	(lizard)	Gt	0...2...0.0	-EXO1	-WDR64	-PIGM	+RGS7	indel	408 aa	000 nm
->ENCEPH_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2...0.0	-ASAH3L	+RPS6	-ADFP	-DENND4C	indel	401 aa	000 nm		encephalopsin	OPN3
+	>ENCEPH_xenTro	Xenopus	tropicalis	(frog)	Gt	0...2...0.0	-ASAH3L	+RPS6	-ADFP	-DENND4C	indel	401 aa	000 nm
->ENCEPH4a_takRub	Takifugu	rubripes	(teleost)	Gt	0...2...0.0	-CALD1	+TNK2	-RAB18	+ABI1	indel	403 aa	000 nm		encephalopsin	TMT
+	>ENCEPH_takRub	Takifugu	rubripes	(teleost)	Gt	0...2...0.0	-ABLIM1	+PTK7	-KMO	+IDE	indel	388 aa	000 nm
->ENCEPH4b_takRub	Takifugu	rubripes	(teleost)	Gt	0...2...0.0					indel	407 aa	000 nm		encephalopsin	OPN3
+	>ENCEPH4a_takRub	Takifugu	rubripes	(teleost)	Gt	0...2...0.0	-CALD1	+TNK2	-RAB18	+ABI1	indel	403 aa	000 nm
->ENCEPH_takRub	Takifugu	rubripes	(teleost)	Gt	0...2...0.0	-	+PTK7	-KMO	+IDE	indel		000 nm
+	>ENCEPH4b_takRub	Takifugu	rubripes	(teleost)	Gt	0...2...0.0	+TFRC	+CHES1	-MYEOV2	-ARHGAP21	indel	407 aa	000 nm
->ENCEPH_tetNig	Tetraodon	nigroviridis	(pufferfish)	Gt	0...2...0.0	-	-	-	-	indel	388 aa	000 nm		encephalopsin	OPN3
+	>ENCEPH_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gt	0...2...0.0	-LDOC1L	+CDC42EP3	-KMO	+IDE	indel	389 aa	000 nm
->ENCEPH_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gt	0...2...0.0		+CDC42EP3	-KMO	+IDE	indel	389 aa	000 nm		encephalopsin	OPN3
+	>ENCEPH4_braFlo	Branchiostoma	floridae	(amphioxus)	Gt		-ZFYVE1	+RTF1	-CES1	-POMT2	indel	402 aa	000 nm
->ENCEPH4_braFlo	Branchiostoma	floridae	(amphioxus)	Gt						indel	402 aa	000 nm		encephalopsin	Amphiop4
+	>ENCEPH4_braBel	Branchiostoma	belcheri	(amphioxus)	Gt		x	x	x	x	indel	401 aa	000 nm
->ENCEPH4_braBel	Branchiostoma	belcheri	(amphioxus)	Gt		x	x	x	x	indel	401 aa	000 nm		encephalopsin	Amphiop4
+	>ENCEPH5_braFlo	Branchiostoma	floridae	(amphioxus)	Gt		-ZFYVE1	+RTF1	+ATP6V0E1	-Etf1	indel	409 aa	000 nm
->ENCEPH5_braBel	Branchiostoma	belcheri	(amphioxus)	Gt		x	x	x	x	indel	421 aa	000 nm		encephalopsin	Amphiop5
+	>ENCEPH5_braBel	Branchiostoma	belcheri	(amphioxus)	Gt		x	x	x	x	indel	421 aa	000 nm
->ENCEPH5_braFlo	Branchiostoma	floridae	(amphioxus)	Gt						indel	409 aa	000 nm		encephalopsin
+	>ENCEPH_apiMel	Apis	mellifera	(bee)	Gt						indel	329 aa	000 nm
->ENCEPH_apiMel	Apis	mellifera	(bee)	Gt						indel	329 aa	000 nm		encephalopsin
+	>ENCEPH1_anoGam	Anopheles	gambiae	(mosquito)	Gt						indel	461 aa	000 nm
->ENCEPH1_anoGam	Anopheles	gambiae	(mosquito)	Gt						indel	461 aa	000 nm		encephalopsin
+	>ENCEPH2_anoGam	Anopheles	gambiae	(mosquito)	Gt						indel	434 aa	000 nm
->ENCEPH2_anoGam	Anopheles	gambiae	(mosquito)	Gt						indel	434 aa	000 nm		encephalopsin
+	>CILL2_plaDum	Platynereis	dumerilii	(ragworm)	Gt		x	x	x	x	indel	310 aa	000 nm
->CILL2_plaDum	Platynereis	dumerilii	(ragworm)	Gt		x	x	x	x	indel	310 aa	000 nm		proto_cilliary
+	>CILL1_plaDum	Platynereis	dumerilii	(ragworm)	Gt		x	x	x	x	indel	355 aa	000 nm
->CILL1_plaDum	Platynereis	dumerilii	(ragworm)	Gt		x	x	x	x	indel	355 aa	000 nm		proto_cilliary
+	>RGR_homSap	Homo	sapiens	(human)	??		+PCDH21	-LRIT1	-GRID1	-WAPAL	indel	296 aa	000 nm
->RGR_homSap	Homo	sapiens	(human)	??		PCDH21	LRRC21	GRID1	WAPAL	indel	296 aa	000 nm		retinal_ganglia	RGR
+	>RGR_galGal	Gallus	gallus	(chicken)	??		+PCDH21	-LRIT1	+CHAT	-PARG	indel	296 aa	000 nm	14985289
->PER_homSap	Homo	sapiens	(human)	??		CFI	NOLA1	EGF	ELOVL6	indel	338 aa	000 nm		peropsin	RRH
+	>RGR_xenTro	Xenopus	tropicalis	(frog)	??		+PCDH21	-LRIT1	+CHAT	-PARG	indel	296 aa	000 nm	-
->PERa_braBel	Branchiostoma	belcheri	(amphioxus)	??		x	x	x	x	indel	365 aa	000 nm		peropsin	Amphiop3
+	>RGR_gasAcu	Gasterosteus	aculeatus	(stickleback)	??		+PCDH21	-LRIT1	+CHAT	-PARG	indel	296 aa	000 nm	-
->PERb_braBel	Branchiostoma	belcheri	(amphioxus)	??		x	x	x	x	indel	522 aa	000 nm		peropsin	Amphiop2
+	>PER_homSap	Homo	sapiens	(human)	??		-CFI	+NOLA1	+EGF	-ELOVL6	indel	338 aa	000 nm
->PERc_braBel	Branchiostoma	belcheri	(amphioxus)	Go		x	x	x	x	indel	391 aa	000 nm		peropsin	Amphiop1
+	>PER_monDom	Monodelphis	domestica	(opossum)	??		-CFI	+NOLA1	+EGF	-ELOVL6	indel	326 aa	000 nm	-
->NEUR_homSap	Homo	sapiens	(human)	??		+CD2AP	+GPR115	-C6orf138	-MUT	indel	355 aa	000 nm		neuropsin	OPN5
+	>PER_galGal	Gallus	gallus	(chicken)	??		-CFI	+NOLA1	+EGF	-ELOVL6	indel	335 aa	000 nm	14985289
->MEL1_homSap	Homo	sapiens	(human)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel	479 aa	000 nm		melanopsin	OPN4
+	>PER_xenTro	Xenopus	tropicalis	(frog)	??		-CFI	+NOLA1	+EGF	-ELOVL6	indel	347 aa	000 nm
->MEL1_monDom	Monodelphis	domestica	(opossum)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel		000 nm		melanopsin	OPN4
+	>PER_gasAcu	Gasterosteus	aculeatus	(stickleback)	??		+GPR68	-GNPDA1	-ENPEP	-C14orf100
->MEL1_galGal	Gallus	gallus	(chicken)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel		000 nm	16856781	melanopsin	OPN4m
+	>PERa_braBel	Branchiostoma	belcheri	(amphioxus)	??		x	x	x	x	indel	365 aa	000 nm
->MEL1_xenTro	Xenopus	tropicalis	(frog)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel	596 aa	000 nm	16856781	melanopsin	OPN4m
+	>PERb_braBel	Branchiostoma	belcheri	(amphioxus)	??		x	x	x	x	indel	522 aa	000 nm
->MEL1_danRer	Danio	rerio	(zebrafish)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm		melanopsin	OPN4m
+	>PERc_braBel	Branchiostoma	belcheri	(amphioxus)	Go		x	x	x	x	indel	391 aa	000 nm
->MEL1D_danRer	Danio	rerio	(zebrafish)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm
+	>NEUR_homSap	Homo	sapiens	(human)	??		+CD2AP	+GPR115	-C6orf138	-MUT	indel	355 aa	000 nm
->MEL1_takRub	Takifugu	rubripes	(teleost)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm		melanopsin	OPN4m
+	>MEL1_homSap	Homo	sapiens	(human)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel	479 aa	000 nm
->MEL1_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gq		-	-	+LDB3	+BMPR1A	indel		000 nm		melanopsin	OPN4m
+	>MEL1_monDom	Monodelphis	domestica	(opossum)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel		000 nm
->MEL1_oryLat	Oryzias	latipes	(medaka)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm		melanopsin	OPN4m
+	>MEL1_galGal	Gallus	gallus	(chicken)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel		000 nm	16856781
->MEL1_calMil	Callorhinchus	milii	(elephantfish)	Gq		-	-	-	-	indel		000 nm		melanopsin	OPN4m
+	>MEL1_xenTro	Xenopus	tropicalis	(frog)	Gq		-GRID1	-WAPAL	+LDB3	+BMPR1A	indel	596 aa	000 nm	16856781
->MEL1a_braFlo	Branchiostoma	floridae	(amphioxus)	Gq		-	-	-	-	indel	709 aa	000 nm		melanopsin	Amphi-mop
+	>MEL1_danRer	Danio	rerio	(zebrafish)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm
->MEL1a_braBel	Branchiostoma	belcheri	(amphioxus)	Gq		x	x	x	x	indel	707 aa	000 nm		melanopsin	Amphi-mop
+	>MEL1D_danRer	Danio	rerio	(zebrafish)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm
->MEL1b_braFlo	Branchiostoma	floridae	(amphioxus)	Gq		-	-	-	-	indel		000 nm
+	>MEL1_takRub	Takifugu	rubripes	(teleost)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm
->MEL1b_braBel	Branchiostoma	belcheri	(amphioxus)	Gq		x	x	x	x	indel	402 aa	000 nm		melanopsin	Amphiop6
+	>MEL1_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gq		-	-	+LDB3	+BMPR1A	indel		000 nm
->MEL2_galGal	Gallus	gallus	(chicken)	Gq		+GRID2+SMARCAD1	-PGDS	-SEC24B	+COL25A1	indel	544 aa	000 nm	17977531	melanopsin
+	>MEL1_oryLat	Oryzias	latipes	(medaka)	Gq		-	+USP54	+LDB3	+BMPR1A	indel		000 nm
->MEL2_anoCar	Anolis	carolinensis	(lizard)	Gq		+GRID2+SMARCAD1	-ATOH1	+PDLIM5	+BMPR1B	indel	290 aa	000 nm		melanopsin
+	>MEL1_calMil	Callorhinchus	milii	(elephantfish)	Gq		-	-	-	-	indel		000 nm
->MEL2_xenLae	Xenopus	laevis	(frog)	Gq		+SMARCAD1		+PDLIM5	+BMPR1B	indel	535 aa	000 nm		melanopsin	Xmop
+	>MEL1a_braFlo	Branchiostoma	floridae	(amphioxus)	Gq		-	-	-	-	indel	709 aa	000 nm
->MEL2_danRer	Danio	rerio	(zebrafish)	Gq		-	+FLJ39155	+PDLIM5	-	indel		000 nm
+	>MEL1a_braBel	Branchiostoma	belcheri	(amphioxus)	Gq		x	x	x	x	indel	707 aa	000 nm
->MEL2_tetNig	Tetraodon	nigroviridis	(pufferfish)	Gq		-	-	-	+BMPR1B	indel	404 aa	000 nm		melanopsin
+	>MEL1b_braFlo	Branchiostoma	floridae	(amphioxus)	Gq		-	-	-	-	indel		000 nm
->MEL2_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gq		KNTC2	+FLJ39155	+PDLIM5	+BMPR1B	indel	353 aa	000 nm		melanopsin
+	>MEL1b_braBel	Branchiostoma	belcheri	(amphioxus)	Gq		x	x	x	x	indel	402 aa	000 nm
->RHAB_plaDum	Platynereis	dumerilii	(polychaete)	Gq		x	x	x	x	indel	383 aa	000 nm		rhabdomeric
+	>MEL2_galGal	Gallus	gallus	(chicken)	Gq		+GRID2+SMARCAD1	-PGDS	-SEC24B	+COL25A1	indel	544 aa	000 nm	17977531
->RHAB1_apiMel	Apis	mellifera	(bee)	Gq						indel	378 aa	000 nm		rhabdomeric	Lop1
+	>MEL2_anoCar	Anolis	carolinensis	(lizard)	Gq		+GRID2+SMARCAD1	-ATOH1	+PDLIM5	+BMPR1B	indel	290 aa	000 nm
->RHAB2_apiMel	Apis	mellifera	(bee)	Gq						indel	386 aa	000 nm		rhabdomeric	Lop2
+	>MEL2_xenLae	Xenopus	laevis	(frog)	Gq		+SMARCAD1		+PDLIM5	+BMPR1B	indel	535 aa	000 nm
->RHAB3_apiMel	Apis	mellifera	(bee)	Gq						indel	372 aa	000 nm		rhabdomeric
+	>MEL2_danRer	Danio	rerio	(zebrafish)	Gq		-	+FLJ39155	+PDLIM5	-	indel		000 nm
->RHAB4_apiMel	Apis	mellifera	(bee)	Gq						indel	378 aa	000 nm		rhabdomeric	Blop
+	>MEL2_tetNig	Tetraodon	nigroviridis	(pufferfish)	Gq		-	-	-	+BMPR1B	indel	404 aa	000 nm
+	>MEL2_gasAcu	Gasterosteus	aculeatus	(stickleback)	Gq		KNTC2	+FLJ39155	+PDLIM5	+BMPR1B	indel	353 aa	000 nm
+	>RHAB_plaDum	Platynereis	dumerilii	(polychaete)	Gq		x	x	x	x	indel	383 aa	000 nm
+	>RHAB1_apiMel	Apis	mellifera	(bee)	Gq						indel	378 aa	000 nm
+	>RHAB2_apiMel	Apis	mellifera	(bee)	Gq						indel	386 aa	000 nm
+	>RHAB3_apiMel	Apis	mellifera	(bee)	Gq						indel	372 aa	000 nm
+	>RHAB4_apiMel	Apis	mellifera	(bee)	Gq						indel	378 aa	000 nm
+	>RHO1_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	complete	000 nm
+	>LWS_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>PIN_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>PPIN_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>VAOP_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>RGR_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>PER_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>ENCEPH_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>ENCEPH4_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>ENCEPH5_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>MEL1b_calMil	Callorhinchus	milii	(elephantfish)			x	x	x	x	indel	fragment	000 nm
+	>ENCEPH_squAca	Squalus	acanthias	(dogfish)			x	x	x	x	indel	fragment	000 nm
+	>ENCEPH_petMar	Petromyzon	marinus	(lamprey)			x	x	x	x	indel	fragment	000 nm
+	>MEL1_petMar	Petromyzon	marinus	(lamprey)			x	x	x	x	indel	fragment	000 nm
+	>PARIE_petMar	Petromyzon	marinus	(lamprey)			x	x	x	x	indel	fragment	000 nm
 </pre>
 <pre>
@@ Line 795: / Line 818: @@
 IRSIQDLQTVQIIKILRYEKKVSVMFFLMISCFLLCWTPYAVVSMMVAFGRRSMVSPTMAIIPSFFAKSSTAYNPLIYVFMSRK 0
 FRHCLLQLLCSRLSWLQRSLKERPLAPVQRPIRPIVMSRPCGKGNRPKKKVTFSSSSIVFIITSDDFGQLDVTSKSGDSADVNAIQVRPL* 0
->ENCEPH_tetNig encephalopsin
-MSSADDSRSARSGEPSLFAVHTYRLLAAAIGAIGVLGFCNNLAVAALYWRFRRLRTPTNLLLLNISLSDLLVSLLGVNFTFAACVQGRWTWNQATCVWDGFSNSLF 1
-GIVSIMTLAALAYERYIRVVHAQVVDFPWAWRAIGHIWLYSLAWTGAPLLGWNRYTLEIHRLGCSLDWASKDPNDASFILLFLLACFFVPVGIMIYCYGNILYAVHM 0
-IRSIQDLQTVQIIKILRYEKKVSVMFFLMISCFLLCWTPYAVVSMMVAFGRKSMVSPTVAIIPSFFAKSSTAYNPVIYVFMSRK 0
-FRRCLLQLLCSRLSWLQRGLKERPLAPVQRPIRPIVVSRPCGKGTRPKKKVTFSSSSIVFIITSDDFRQLDVTSRAGDSADVNAIQVRPL* 0
 >ENCEPH_gasAcu Gasterosteus aculeatus (stickleback) encephalopsin
@@ Line 808: / Line 825: @@
 FRRCLMQLLCSRVTCLQCNLKERPLAPVQRPIRPIVVSAACGGGRVRPKKRVTFSSSSIVFIITRNDIRHTDVTSNTRESSEANVFQVRPL* 0
->ENCEPH4_braFlo Branchiostoma floridae Amphiop4 AB050608 Un - 207841137 207851157 10021 new exon12 and 34 + perfect fit +CES1 -ENC -RTF1
+>ENCEPH4_braFlo Branchiostoma floridae Amphiop4 AB050608 Un - 207841137 207851157 10021 new exon12 and 34 + perfect fit +CES1 -ENC -RTF1 TMT-opsin takRub
 MALYNNTSSPSQDLLWDAPYSQGHIWDNSSASNSSEDVMDQGKVELQDFSDAGYTAIATCLALI 1
 GFVGFTNNFVVILLIGCHRQLRTPFNLLLLNMSVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANSLF 1
@@ Line 823: / Line 840: @@
 SAEMKSEFGNPQNTGRLVTTMVVVMIVCFLVCWTPYTVMALIVTFGADHLVTPTASVIPSLVAKSSTAYNPIIYVLMNNQ 0
 FREFLLARLRTFCCRQPRMLRVTPMDDNAHARLVGEGPSHAQQVIPSEENGENVEMRKVQGNQLKADSLSTISE* 0
+>ENCEPH5_braFlo Branchiostoma floridae extra 00 intron chrUn:398,023,923-398,103,839 +ETF1 -ENC -RTF1
+MLGMHNVMNATDYDNNNATFAAWNFQRNGTTEEEVEFSGFDTVAVVIAAIGIAGFLSNGAVVLLFLKFRQLRTPFNMLLLNMSVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANHLF 1
+GLVSLISLAVISYERYRMVVKPKGPGSSYLTYNKVGLAIIFIYLYCLLWTTLPIVGWSSYQLE 0
+GPKISCSVAWEEHSLSNTSYIVAIFIMCLLLPLLIIIYSYCRLWYKVKK 0
+GSQNLPPAIRKSSQKEQKIARMVVVMITCFLVCWLPYGAMALVVSFGGESLISPTAAVVPSLLAKSSTCYNPLVYFAMNNQ 0
+FRRYFQDLLCCGRRLFDASASVNTCNTSAMPRHSPVFQKPDSDQYNGIQKSREPQMRTTGQNAPYRQWIEMQTIAVVVKADEVNNKFGEVKT* 0
 >ENCEPH5_braBel Branchiostoma belcheri Amphiop5 AB050609 early dup of ENCEPH extra Nfrag in mrna
@@ Line 830: / Line 854: @@
 GSRNLPAAMRKSSQKEQKIAMMVIVMITCFMVCWLPYGAMALVVTFGGERLISHTAAVVPSLLAKSSTCYNPVVYFAMNSQ 0
 FRRYFQDLLCCGRRLFDVSQSVVTGNTAMPRNNSQGFRKDDSDQKQDNGLPKQSEGPMCDHSSNESQMEGSRHNTAASQQWIEMQTIAVVVKAVEVDTSAANEP* 0
->ENCEPH5_braFlo Branchiostoma floridae extra 00 intron chrUn:398,023,923-398,103,839 +ETF1 -ENC -RTF1
-MLGMHNVMNATDYDNNNATFAAWNFQRNGTTEEEVEFSGFDTVAVVIAAIGIAGFLSNGAVVLLFLKFRQLRTPFNMLLLNMSVADLLVSVCGNTLSFASAVRHRWLWGRPGCVWYGFANHLF 1
-GLVSLISLAVISYERYRMVVKPKGPGSSYLTYNKVGLAIIFIYLYCLLWTTLPIVGWSSYQLE 0
-GPKISCSVAWEEHSLSNTSYIVAIFIMCLLLPLLIIIYSYCRLWYKVKK 0
-GSQNLPPAIRKSSQKEQKIARMVVVMITCFLVCWLPYGAMALVVSFGGESLISPTAAVVPSLLAKSSTCYNPLVYFAMNNQ 0
-FRRYFQDLLCCGRRLFDASASVNTCNTSAMPRHSPVFQKPDSDQYNGIQKSREPQMRTTGQNAPYRQWIEMQTIAVVVKADEVNNKFGEVKT* 0
 >ENCEPH_apiMel Apis mellifera (honey bee) NM_001039968 328 aa ciliary opsin Gt pteropsin clock -ENC -PSMA3
@@ Line 884: / Line 901: @@
 VNTTLPARTLLLGWGPYAILYLYAVIADVTSISPKLQM 0
 VPALIAKMVPTINAINYALGNEMVCRGIWQCLSPQKREKDRTK* 0
+>RGR_galGal Gallus gallus (chicken) Opsin photoisomerases in the chick retina and pineal gland 14985289 NM_001031216
+MVTSHPLPEGFTEIEVFAIGTALLVE 1
+ALLGFCLNGLTIISFRKIKELRTPSNLLVLSIALADCGICINAFIAAFSSFLR 2
+YWPYGSEGCQIHGFQGFLTALASISSSAAVAWDRYHHYCT 1
+RSKLQWSTAISMMVFAWLFAAFWATMPLLGWGEYDYEPLRTCCTLDYSKGDR 2
+NYITFLFALSIFNFMIPGFIMMTAYQSIHQKFKKSGHYK 0
+FNTGLPLKTLVICWGPYCLLSFYAAIENVMFISPKYRM 0
+IPAIIAKTVPTVDSFVYALGNENYRGGIWQFLTGQKIEKAEVDSKTK* 0
+>RGR_xenTro
+MVTSYPLPEGFTETEVFAIGTTLLVE 0
+ALLGLLLNGLTLLSFYKIRELRTPSNLFIISLAVADTGLCLNAFVAAFSSFLR 2
+YWPYGSEGCQIHGFQGFVAALSSIGSCAAIAWDRYHQYCT 1
+RSKLHWSTAVSVVFFIWGFSAFWSAMPLFGWGEYDYEPLRTCCTLDYSKGDR 2
+NYISYLFTMAFFEFLVPLFILMTAYQSIYQKMKKSGQIR 0
+FNTSMPVKSLVFCWGPYCLLCFYAVIQDATILSPKLRM 0
+IPALLAKTSPAVNAYVYGLGNENYRGGIWQYLTGQKLEKAETDNKTK* 0
+>RGR_gasAcu stickleback DN680995
+MVSSYPLPDGFTDFDVFSLGSCLLVE 0
+GLLGILLNAVTIAAFLKVRELRTPSNFLVFSLAVADIGISMNATIAAFSSFLR 2
+YWPYGSDGCQTHGFQGFVTALASIHFIAAIAWDRYHQYCT 1
+RTKLQWSSAITLAVFVWLFTAFWSAMPLIGWGEYDYEPLRTCCTLDYTKGDR 2
+NYVSYLIPMAIFNMAIQVFVVMSSYQSIAQKFKKTGNPR 0
+FNPNTPLKAMLFCWGPYGILAFYAAVENATLVSTKLRM 0
+MAPILAKTSPTFNVFLYALGNENYRGGIWQLLTGEKIDVPQIENKSK* 0
 >PER_homSap peropsin RRH 4q25 7 chr4:110,968,599-110,985,310 337 + 16712 retinal photoisomerase Retinal pigment epithelium
@@ Line 894: / Line 938: @@
 FRRAMLAMFKCQTHQTMPVTSILPMDVSQNPLASGRI* 0
->PERa_braBel Branchiostoma belcheri Amphiop3 peropsin AB050610
+>PER_monDom Monodelphis domestica (opossum)
-MDIPTETPYGAEEDIGESAGWRWTETDKNGFHKYDHLIVGLYLFVIGIIGTIENGITLATFSKFRSLRSPTTMLLVHLAI
+MFKNNSVKTLAPEKEGPSVFSPIEHKIVAAYLITA 1
-ADLGICIFGYPFSGASSLRSHWLFGGVGCQWYGFNGMFFGMANIGLLTCVAVDRYLVICRHDLVDKVNYNTYGVMAALGW
+GVISIVSNVIVLGIFVKYKALRTATNTIIINLAVTDIGVSSIGYPMSAASDLYGSWKFGYDGCQ 0
-LFAAFWAALPLVGWAEYALEPSGTACTINFQKNDSLYISYVTSCFVLGFVVPLAVMAFCYWQASCFVSKVLKGDIAGDLT
+IYAGLNIFFGMASIGLLTAVAIDRYLTICQPDL 1
-FPVAANVDWEYQNHFSKMCLAMVAAFVVAWTPYSVLFLFAAFWNPADIPAWLTLLPPLIAKSSALYNPIIYIIANRR FRNAICSMMKGQDPDVEDDEHADEHRVRSIEDNDKEIISMVNLNMTV*
+GRMTSYNYTLMILTAWVNGFFWALMPIVGWAGYAPDPTGATCTINWRKNDV 2
+SFVSYTMTVITINFAMPLGVMFYCYYNVSQKMKQYSPSNCPDHINRDWSNQVAVTK 0
+MSVVMILMFLLAWSPYSIVCLWASFGDPKEIPPAMAIVAPLFAKSSTFYNPCIYVAANKK 2
+FRRAISAMIRCQTHQSMPISNALPMN* 0
+>PER_galGal Gallus gallus (chicken)
+MHWNDSANSSESDAEAHSVFTQTEHNIVAAYLITA 1
+GVISIFSNIVVLGIFVKYKEFRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYTGCQ 0
+IYAALNIFFGMASIGLLTVVAVDRYLTICRPDI 1
+GRRMTTRNYAALILAAWINAVFWASMPTVGWAGYASDPTGATCTANWRKNDV 2
+PFVSYTMSVIAVNFVVPLTVMFYCYYNVSRTMKQYTSSNCLESINMDWSDQVDVTK 0
+MSVVMIVMFLVAWSPYSIVCLWSSFGDPKKISPAMAIIAPLFAKSSTFYNPCIYVIANKK 2
+FRRAILAMVRCQTRQEITISNALPMTVSLSALTS* 0
+>PER_xenTro Xenopus tropicalis (frog)
+METLAEVSTLLPAGTGTVNISDASSEVHSVFSQSEHNIVAAYLITA 1
+GVISILSNIIVLGIFVKYKELRTATNAIIINLAFTDIGVSGIGYPMSAASDLHGSWKFGYVGCQ 0
+IYAGLNIFFGMASIGLLTVVAIDRYLTICRPDIG 1
+GRRISGRHYTAMILAAWINAVFWSVMPVVGWSSYAPDPTGATCTINWRKNDV 2
+SFVSYTMSVVAVNFVVPLMVMFYCYYNVSRTMKGYGSRSSLGGINADWSDQTDVTK 0
+MSMVMIVMFLVAWSPYSIVCLWSSFGDPRKIPPAMAIIAPLFAKSSTFYNPCIYVIANKK 2
+FRRAILSMVQCKSRQEVTLDNHFPMNVSQSTLTT* 0
+>PER_gasAcu Gasterosteus aculeatus (stickleback)
+MGIDPEVNVTDDVTLYGGKSAFTQLEHNIVAGYLITA 1
+GVISLFSNIVVLLMFWKFKELRTATNFIIINLAFTDIGVAGIGYPMSAASDIHGSWKFGYAGCQ 0
+IYAALNIFFGMASIGLLTVVAIDRYLTICRPDIG 1
+GQKMTMQSYNLLILAAWLNAVFWSSMPVVGWASYAPDPTGATCTINWRQNDV 2
+SFISYTMAVIAVNFVLPLSAMFYCYYNVSATVKRYKASNCLDSANIDWSDQMDVTK 0
+MSIVMIIMFLVAWSPYSIVCLWASFGDPKTIPAPMAIIAPLFAKSSTFYNPCIYVIANKK 2
+FRRAIIGMVRCQTRQRITINSQVPMTTSQQPLTQ* 0
+>PER_braBel Branchiostoma belcheri Amphiop3 peropsin AB050610
+MDIPTETPYGAEEDIGESAGWRWTETDKNGFHKYDHLIVGLYLFVI
+GIIGTIENGITLATFSKFRSLRSPTTMLLVHLAIADLGICIFGYPFSGASSLRSHWLFGGVGCQ 0
+WYGFNGMFFGMANIGLLTCVAVDRYLVICRHDLV
+DKVNYNTYGVMAALGWLFAAFWAALPLVGWAEYALEPSGTACTINFQKNDS 2
+LYISYVTSCFVLGFVVPLAVMAFCYWQASCFVSKVLKGDIAGDLTFPVAANVDWEYQ
+NHFSKMCLAMVAAFVVAWTPYSVLFLFAAFWNPADIPAWLTLLPPLIAKSSALYNPIIYIIANRR 0
+FRNAICSMMKGQDPDVEDDEHADEHRVRSIEDNDKEIISMVNLNMTV* 0
 >PERb_braBel Branchiostoma belcheri Amphiop2 peropsin RRH AB050607
@@ Line 1,191: / Line 1,274: @@
 IYAINHPRYRQELQKRCKWMGIHEPETTSDATSAQTEKIKTDE*
-</pre>
-Here are some fragmentary opsins from key evolutionary groups that I recovered from ongoing genome projects
-<pre>
 >RHO1_calMil Callorhinchus milii complete wgs cannot find cone opsins
 MNGTEGENFYIPMSNKTGVVRSPFEYPQYYLAEPWQFSILAAYMFFLIITCFPVNFLTLYVTFEHKKLRQPLNFILLNLAVADLFMVFGGFFITVYTSLHGYFVFGVTGCNFEGFFATLG 1
@@ Line 1,218: / Line 1,298: @@
 >VAOP_calMil Callorhinchus milii fragment
 VASTQGRLGVARKPEKQVTRMVIVMILAFLFCWTPYAAFSITVTACPTIKLDPRLAAIPAFFSKTATVYNPIIYVFMNKQ 0
+>RGR_calMil Callorhinchus milii
+    EGFTDFEVFGLGTALLVE 0
+GLVGLLLNGLTLLAFYKIKELRTPSNLLITSLALSDFGISMNAFIAAFSSFLR 2
+YWPYGSEGCQTHGFHGFLMALASINACAAIAWDRYHQNCS 1
+SRLQWSSAITVTVFIWGIAAFWSAMPLLGWGVYDYEPLRTCCTLDYSKGDR 2
+  EFIFPIFIMLSSYQSCKSKFKKTGQVK 0
+FNTGLPVKTLIFCWGPYSLLCFYATIENITILSPKLRM 0
+>PER_calMil Callorhinchus milii
+LFVSYTMTVIAVNFVVPLSVMFFCYYNVSKTMSRFISSPSPENINLDWSDQLDVTK 0
+MSVVMIVMFLLAWSPYSIVCLWASFGNPKLIPPAMAIIAPLFAKSSTFYNPCIYVIANKK 2
+FRKAIMAMICCQNRQEITINHTLPMTISRVPLTE* 0
 >ENCEPH_calMil Callorhinchus milii 1099306574966 mainstream fragment