Sulfatase evolution: ARSK
Introduction to sulfatases
Sulfatases are an old and deeply diverged family of hydratases that remove sulfate moieties from a variety of small and large molecules. Despite the apparent simplicity of this reaction, the sulfatase domain fold is perhaps the largest known for any enzyme and an unprecedented formyl glycine post-translational modification of encoded cysteine, serine or threonine is critical to activity. The fold is closely related to that of alkaline phosphatases, though primary sequence alignability has almost completely dissipated.
The 17 human paralogs reside either in lysozomes or endoplasmic reticulum. Mutations in these genes result in diseases that provide important clues as to natural substrates (which accumulate in lysosomal storage diseases). However only 8 of the 17 genes have an associated disease at OMIM as of Sept 2010. Functions of the remaining sulfatases have yet to be discovered, perhaps because the accumulating metabolite is not toxic or has an alternative catabolic pathway. Such diseases could be recessive and hence rare in the case of unassigned autosomal sulfatases.
ARSK is such a gene. First described in 2003 as SULFX, the substrate and function of ARSK remain unknown -- it has not yet been the focus of a single experimental paper. ARSK is a fairly typical sulfatase of 536 amino acids encoded by eight exons on human chr 5 with a conventional CPSRA formylglycine motif. It lacks overt membrane insertional regions and GPI terminal motif so is presumably soluble.
ARSK is however peculiar in several bioinformatic respects. Although clearly a full length duplicate of an ancestral sulfatase, its opaque evolutionary relationship to non-orthologous sulfatases makes it difficult to place in the sulfate gene tree. Its closest affinity (percent identity low 20's) is perhaps with IDS which removes the sulfate from iduronate, though the ARSK substrate may have drifted off to something else entirely during the 600+ million years since gene duplication.
A second unusual feature is that the 7 introns within the coding region of ARSK do not bear any relationship in position or phase to those of other human sulfatases. This suggests that the gene duplication event leading to ARSK and other sulfatases preceded the main era of gene intronation, ie sulfatses initially had no introns (as in bacterial genes) and were subsequently independently intronated in early eukaryotes. Once established, the introns of ARSK have been stable over billions of years of gene tree branch length.
The phylogenetic distribution of ARSK also raises many questions. Within deuterostomes, orthologs are readily located in representatives of all major subclades with the exception of echinoderms and tunicates. ARSK has evolved quite conservatively here, with the human protein still having 54% and 52% identity over 500 residues to Branchiostoma (amphioxus) and Saccoglossus (acornworm) respectively, despite divergences that preceded the Cambrian. Intron positions and phases are precisely preserved beyond two minor fission events, leaving no doubt of orthology within deuterostomes. However, ARSK is otherwise completely missing from other eumetazoans (ecdysozoa, lophotrochozoa, and cnidaria). Also missing from Trichoplax and sponge genomes, it makes its last eukaryotic appearance in Monosiga, a marine choanoflagellate, before fading into bacterial sequences of uncertain affinities.
A final oddity of ARSK observed early on is its close proximity to an apparently unrelated gene, TTC37 (twenty tetratricopeptide repeats 37): only 144 bp separate the two genes. These are transcribed divergently and could well share a bidirection promoter or overlap in 5' UTR. This relationship is by no means restricted to the human genes -- it is readily traced back throughout vertebrates. The putative chaperone function of TTC37 remains unspecified, though in June 2010 a disease has been assigned to it: trichohepatoenteric syndrome (THES) -- an "autosomal-recessive disorder characterized by life-threatening diarrhea in infancy, immunodeficiency, liver disease, trichorrhexis nodosa, facial dysmorphism, hypopigmentation, and cardiac defects". This does not immediately suggest why ARSK and TTC37 should be so closely linked.
ARSK phylogenetic distribution
As noted above, ARSK has evolved with above-average conservation in deuterostomes and especially vertebrates. Its apparent loss in echinoderms and tunicates -- which in itself is not unusual -- might be overturned if more genomes were sequenced from these clades. Its occurence in all earlier diverging lineages is restricted to Monosiga, which requires several independent losses as the species tree topology now stands. Monosiga thus illustrates the importance of thorough genomic sampling.
However even without the Monosiga sequence, it is certain that ARSK did not arise in deuterostomes either by horizontal gene transfer (from bacteria), nor from gene duplication and rapid divergence from a sulfatase exising in the bilateran ancestor, nor de nov from say junk dna. These options are all ruled out by its 7 immensely conserved GT-AG coding introns that do not resemble anything from these other sources. (A very high percentage of exons are precisely conserved between human and sponge implying the main era of intron creation occured much earlier.)
The Monosiga sequence below does have some problematic aspects. In part these arise from the poor quality pipeline model XM_001747506 posted to GenBank that skipped over the small exon containing the catalytic site motif CPSRT as well as omitting a long distal exon. These missing exons are easily located using Blastx of the enveloping contig ABFJ01000822 against a small database of validated ARSK orthologs. However ambiguity remaining in two earlier exons with weak homology can only be resolved by transcript sequencing.
Despite these uncertainties, it is clear that intron positions and phases do not match those of deuterostomes very well. In particular, the latter all have a phase 1 intron starting one residue after the CCPSR motif. This motif and the following WSG pattern are easily recognized in Monosiga but there is no possibility of a GT-AG intron in the anticipated position:
agcccctgtatgctgtcccagccgaacttcgacttggtcgggccgtcacgt (The red tc shows deuterostome GT splice donor position.) A P V C C P S R T S T W S G R H
Despite unresolved intron issues, back-Blastp of Monosiga to the 17 sulfatases of human at GenBank gives ARSK_homSap as best match by a wide margin. When the target is all 'non-redundant' deuterostome sequences at GenBank, the best match is amphioxus ARSK_braFlo followed by a long list of ARSK in other species. After these, much weaker matches to the IDS sulfate appear.
Curiously, when Blastp of Monosiga (or any bona fide ARSK) is restricted to bacteria (which are rich in sulfatases), the top matches are typically annotated as IDS-like or choline-sulfatase. This is consistent with a deep ancestral ARSK/IDS gene whose substrate was (and still is, in bacteria) choline sulfate. After gene duplication and divergence, one copy changed its substrate over time in eukaryotes to iduronate (ie become IDS). The other copy became ARSK; its substrate today is unknown but might well still be choline sulfate. Later that molecule stopped being made in various lineages (eg arthropods) or was alternatively metabolized more effectively, resulting in the subsequent loss of ARSK in those lineages (under the evolutionary principle of 'use it or lose it').
ARSK reference sequences
>ARSK_homSap Homo sapiens (human) 544 aa 8 exons 0 MLLLWVSVVAALALAVLAPGAGEQRRRAAKAPNVVLVVSDSF 0 0 DGRLTFHPGSQVVKLPFINFMKTRGTSFLNAYTNSPICCPSRA 1 2 AMWSGLFTHLTESWNNFKGLDPNYTTWMDVMERHGYRTQKFGKLDYTSGHHSIS 2 1 NRVEAWTRDVAFLLRQEGRPMVNLIRNRTKVRVMERDWQNTDKAVNWLRKEAINYTEPFVIYLGLNLPHPYPSPSSGENFGSSTFHTSLYWLEK 00 VSHDAIKIPKWSPLSEMHPVDYYSSYTKNCTGRFTKKEIKNIRAFYYAMCAETDAML 1 2 GEIILALHQLDLLQKTIVIYSSDHGELAMEHRQFYKMSMYEASAHVPLLMMGPGIKAGLQVSNVVSLVDIYPTML 1 2 DIAGIPLPQNLSGYSLLPLSSETFKNEHKVKNLHPPWILSEFHGCNVNASTYMLRTNHWKYIAYSDGASILPQLF 1 2 DLSSDPDELTNVAVKFPEITYSLDQKLHSIINYPKVSASVHQYNKEQFIKWKQSIGQNYSNVIANLRWHQDWQKEPRKYENAIDQWLKTHMNPRAV* 0 >ARSK_galGal (chicken) NM_001031415 0 MGSGGPLLLLRGLLLVGAAYCAAPRPPRHSSRPNVLLVACDSF 0 0 DGRLTFYPGNQTVDLPFINFMKRHGSVFLNAYTNSPICCPSRA 1 2 AMWSGLFTHLTESWNNFKGLDPDYVTWMDLMQKHGYYTQKYGKLDYTSGHHSVS 2 1 NRVEAWTRDVEFLLRQEGRPKVNLTGDRRHVRVMKTDWQVTDKAVTWIKKEAVNLTQPFALYLGLNLPHPYPSPYAGENFGSSTFLTSPYWLEK 00 VKYEAIKIPTWTALSEMHPVDYYSSYTKNCTGEFTKQEVRRIRAFYYAMCAETDAML 1 2 GEIISALQDTDLLKKTIIMFTSDHGELAMEHRQFYKMSMYEGSSHVPLLVMGPGIRKQQQVSAVVSLVDIYPTML 1 2 DLARIPVLQNLSGYSLLPLLLEKAEDEVPRRGPRPSWVLSEFHGCNVNASTYMLRTDQWKYITYSDGVSVPPQLF 1 2 DLSADPDELTNVAIKFPETVQSLDKILRSIVNYPKVSSTVQNYNKKQFISWKQSLGQNYSNVIANLRWHQDWLKEPKKYEDAIDRWLSQREQRK* 0 >ARSK_xenLae Xenopus laevis (frog) 0 MIQKCIALSLFLFSALPEDNIVRALSLSPNNPKSNVVMVMSDAF 0 0 DGRLTLLPENGLVSLPYINFMKKHGALFLNAYTNSPICCPSRA 1 2 AMWSGLFPHLTESWNNYKCLDSDYPTWMDIVEKNGYVTQRLGKQDYKSGSHSLS 2 1 NRVEAWTRDVPFLLRQEGRPCANLTGNKTQTRVMALDWKNVDTATAWIQKAAQNHSQPFFLYLGLNLPHPYPSETMGENFGSSTFLTSPYWLQK 00 VPYKNVTIPKWKPLQSMHPVDYYSSYTKNCTAPFTEQEIRDIRAYYYAMCAEADGLL 1 2 GEIISALNDTGLLGRTYVVFTSDHGELAMEHRQFYKMSMYEGSSHIPLLIMGPRISPGQQISTVVSLVDLYPTML 1 2 EIAGVQIPQNISGYSLMPLLSASSNKNVSPSISVHPNWAMSEFHGSDANASTYMLWDNYWKYVAYADGDSVAPQLF 1 2 DLSSDPDELTNVAGQVPEKVQEMDKKLRSIVDYPKVSASVHVYNKQQFALWKASVGANYTNVIANLRWHADWNKRPRAYEMAIEKWIKSTRQH* 0 >ARSK_takRub Takifugu rubripes (fugu) 8 exons 504 aa single copy retained after whole genome duplication 0 MSVKLSALILLFLAFHQVLARNRTRPNFLVVMSDAF 0 0 DGRLTFDPGSKVVKLPFINYLRELGVTFINAYTNSPICCPSRA 1 2 AMWSGQFVHLTQSWNNYKCLDANATTWMDLLEVNGYLTKMMGKLDYTSGSHSvs 0 1 NRVEAWTRDVQFLLRQEGRPVTQLVGNMSTVRIMGKDWENIDKATQWIQQRAESSQQPFALYLGLNLPHPYKTESLGPTAGGSTFRTSPHWLEK 00 VSSEHVTVPKWLPGAAMHPVDFYSTFTKNCSGFFTEEEIMNIRAFYYAMCAEADAML 1 2 GQLISALRETHLLNNTVVIFTADHGELAMEHRQFYKMSMFEGSSHVPLLFMGPGLMSGVEADQLVSLVDIYPTVL 1 2 DLADVPPVGSLSGYSLLPLLSTCSSCPGRPHPDWVLSEYHGCNANASTYMLRSGRWKYIAYADGLRVPPQLF 1 2 DMILDKEELHNVVFKFSEVSAQLDKLLRSIVHYPEVSAAVHRYNKESFVAWRHTLGRNYSQVISSLRWHVDWQRNPLANERAIDEWLYGSF* 0 >ARSK_braFlo Branchiostoma floridae (amphioxus) XM_002594507 0 MRMKLDCSAGFLLFWWFTSAVGGTRDDRKNIVFVICDSM 0 0 DGRLIGRGQDSVVDLPNLNYMVQNGVNFRSTYTNSPICCPSRS 1 2 ALWSGLHTHVTQSWNNYKGLPKNYPTWQVRLEQQGYHTQVYGKTDYVSGDHSES 2 1 NRVEAWTRNVNFTLAQEGRPTPVLV 12 GSSSTDRIQLKDWASTDLASHWLLHEAPKQQKPWLLYLGLNLPHPYPTPSMGKNFGGSTFMTSPYWLKK VNSSKVTIPKWLPFSRMHPVDYYSSATKNCTSDFTRDEIMKIREYYYGMCAETDAML 1 2 GQVLDALKASGQADSTYVFFTSDHGELAMEHRQFYKMSMYEASAHIPMVLTGPEVPAGKAVDDLTSLVDVFPTFM 1 2 DIANASQPPGLNGTSLLPLLRNSSDRVDRPDWVLSQYHGCNVNMSTYMLRTGSLKYVAFGDGPNQVSSQLF 1 2 DLDKDPDELHNLAEERQDLASQLDDKLRKLVDYPTVTREVQKYNRDSFMAWKAKLGSRYKDEIANLRWWKDWQKDPQGNQEKVEEWLNNVVS* 0 >ARSK_sacKow Saccoglossus kowalevskii (acornworm) XM_002732823 0 MFSMMQSSILITVLLFTCTCIPRGNEGKPNNVLFIICDAM 0 0 DGRLVGNNLTAVNMANINNRLVSHGVTFTNAYTNSPICCPSRS 1 2 ALWSGLYTHITHAWNNHEGLPADYPTWKIKLEKAGYDSKILGKTDYVSGRHTLS 2 1 NRVEAWTRNVNFTLAQEGRPTPVLVGNKTTIRVKDVDWDNIDKAKDWLENRKSSKATKPFLLYIGINLPHPYSTPGEGEHPGGSTFMTSPYW LQYVDMSKVTIPKWTPLDKMHPVDYYESATKNCTSHFTKDEIRKIRAYYYGMCAEVDGMV 1 2 GEILDQLDSLGLTNTTQVIFTSDHGEMAMEHRQFYKMTMYEASSHVPLIITNPTVPSRQGVAVNDPVSLVDIFPTLM 1 2 DMAAIHHPVGLNGTSLMPYLEGKSHVKKPDWVLSQYHGCNVNMSAYMLRRQEWKYITYGNGKQVAPHLF 1 2 NLDEDPDELHDYANERHDIIAEMDNKLRSIIDYADITNEVSRYNKESFSSWKTSIGDKYSDTIANLRWWKDWQKDPNGNEQRIEEWLKSVE* 0 >ARSK?_monBre Monosiga brevicollis ABFJ01000822 XM_001747506 bad gene model 0 MGNPIRGGSLLIVAASLLVCATLGTAKQPNILFVIDESTDAKAYFAKNPEKAPMPLPNLR 0 0 VPAHVMNSYHYHVRRQSAFFLPDASPCMLSQPNFDL 0 0 APVCCPSRTSTWSGRH 0 0 FVTGAWNNYEGLPE 0 0 NYDLKYSDVLHKGGYNVGIFGKTDFTAGGHTVDARVTAWTNKVNFPFTLQNGSAGWYDETGPLVRTVNVSK 0 0 VVHVSDWNHANQTAKFIADAATHDEPWLAYVGFDIVHP 1 2 NYVSSPYWLDQVDMDKVTVPEWIPLDQLHPEDFQATMKKNMANLTHDPAFIKSVRQHYYGMIAE 2 1 YDAILGVVLDAVEASGEADNTY 0 0 IFVTSDHGDMNMEHQQYYK 0 0 MTYYDPSARVPLIVTGPTVQANVTYENLTSHLDFFPTFLELANV 1 2 VQLEGRSLVPILRTGVDAGRPNVALSQFHGDEIHLSWFMI 1 2 RKDDYKYVTFGSGKEVAPRLFNMREDPLEMNDLAPSNPSLVAELDAELRSYWDYPSIASTAESYNK 1 2 DSFALLRASFNDEDKFKAYLATLRWSTSWSYDPEGSYAAIEAWLKTPNSTFEWAFP* 0
