User:Fan Liu

Fan Liu

Address: 1467 OMAHA RD, North Brunswick, NJ, 08902 E-mail: liuf1@mskcc.org Phone: (646) 888-2158 (Office), (732) 672-0737 (Mobil)

Education:

Ph.D in Biomedical Sciences, Department of Molecular Genetics & Microbiology, Health Science Center, School of Medicine, University of New Mexico at Albuquerque, 08/2000 to 12/2005.

M.S in Pathology, Department of Pathology, Capital University of Medical Sciences, Beijing, P.R. China, 08/1994 to 08/1997.

M.D in Stomatology, Department of Stomatology, Tianjin University of Medical Sciences, Tianjin, P.R. China, 08/1987 to 08/1992.

Work Experience:

Research Associate in Biomedical Sciences, Department of Molecular Pharmacology & Chemistry, Memorial Sloan-Kettering Cancer Center, New York City, 07/2011 to present

Research Fellow in Biomedical Sciences, Department of Molecular Pharmacology & Chemistry, Memorial Sloan-Kettering Cancer Center, New York City, 10/2006 to 07/2011

Postdoctoral Fellow in Biomedical Sciences, Department of Molecular Genetics & Microbiology, Health Science Center, School of Medicine, University of New Mexico at Albuquerque, NM, 01/2006 to 09/2006

Research Assistant in Experimental Pathology, Department of Experimental Pathology, Beijing Friendship Hospital, Beijing, P.R. China, 08/1997 to 08/2000.

Dentist in Dental Department of Dongli Hospital, Tianjin, P.R. China, 08/1992 to 08/1994.

Research Experience:

2007 to present: Memorial Sloan-Kettering Cancer Center, New York. Mentor, Stephen Nimer, MD

• Investigate how the MPN (Myeloproliferative Neoplasm)-associated mutant JAK2 (JAK2V617F) gains the function of regulating PRMT5 (protein arginine methyltransferase 5) activity, and subsequently controlling chromatin modifications and transcription profiles in hematopoietic cells.

• Determine the function of protein arginine methylation in signal transduction pathways.

• Investigate the role of PRMT5 in normal and malignant hematopoiesis using PRMT5 conditional knockout mouse.

• Techniques used: examining protein-protein interactions by co-immunoprecipitation, immunofluesence confocal microscopy; phosphorylation of protein detected by in vitro kinase assay and phosphor-specific antibody; human CD34+ cell isolation, FACS, in vitro culture and retro- or lenti-virus-mediated RNAi system; ChiP-Seq, PRMT5 conditional knockout mouse and MPN animal models.

2006: University of New Mexico Health Science Center, Albuquerque, NM. Mentor, Scott A Ness, Ph.D.

• Investigate the role of post-translational modifications, such as acetylation, in the transcriptional specificity of c-Myb.

• Techniques used: analysis of post-translational modifications by site-directed mutagenesis, immunoprecipitation and proteomics; transcriptional specificity examined by Northern, quantitative real-time PCR and microarray assay.

2001 to 2005: University of New Mexico Health Science Center, Albuquerque, NM Mentor, Scott A Ness, Ph.D.

• Investigate the role of mutations of v-Myb in revealing its oncogenic potential by comparing the transcriptional specificity of v-Myb and c-Myb in both human mammary carcinoma cells and primary peripheral monocytes.

• Techniques used: tissue culture; lentiviral and adenoviral gene transfer to primary human cells; gene expression analysis by Northern blotting, Western blotting, quantitative real-time PCR and microarray assay; transcription factor analysis by chromatin Immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA) and reporter gene assay.

1995 to 1997: Capital University of Medical Sciences, Department of Pathology, Beijing Mentor, Ruifen Chen.

• Examine the pathologic changes in endothelial cells of pulmonary arteriae induced by chronic hypoxia in rats. Investigate the role of NOS (Nitric Oxide Synthetase) in pulmonary hypertension.

• Techniques used: hypoxia-induced pulmonary hypertension animal model, analysis of NOS expression by immunohistochemistry; examining the pathological changes of endothelial cells by electron microscopy, paraffin and frozen tissue sections;

Publications (in chronological order):

• Liu F and Chen RF (1999) Mechanism of pulmonary vascular structure remodeling induced by chronic hypoxia. J. of Capital University of Medical Sciences. 20 (1): 28-31.

• Liu F and Chen RF. (1999) Alteration of NOS expression and activity in pulmonary arterials induced by chronic hypoxia. Chinese J. of Experimental and Clinical Pathology. 15 (6): 512-15.

• Liu F and Ness SA (2004). The v-Myb oncogene. In Myb Transcription Factors: Their Role in Growth, Differentiation and Disease, J. Frampton, ed. (Kluwer Academic Publishers B.V.)

• Lei W, Ruston JJ, Davis JM, Liu F and Ness SA (2004). Positive and negative determinants of target gene specificity in myb transcription factors. J Biol Chem. 279(28): 29519-27.

• Lei W, Liu F, Ness SA (2005). Positive and negative regulation of c-Myb by Cyclin D1, cyclin-dependent kinases and p27 Kip1. Blood. 105(10): 3855-61.

• Liu F, Lei W, O’Rouke J and Ness SA (2006). Oncogenic mutations cause dramatic, qualitative changes in the transcriptional activity of c-Myb. Oncogene 25(5):795-805.

• Perna F, Gurvich N, Hoya-Arias R, Abdel-Wahab O, Levine RL, Asai T, Voza F, Menendez S, Wang L, Liu F, Zhao X, Nimer SD (2010). Depletion of L3MBTL1 promotes the erythroid differentiation of human hematopoietic progenitor cells: possible role in 20q- polycythemia vera. Blood 116(15):2812-21.

• Quintana AM, Liu F, O'Rourke JP, Ness SA. (2011) Identification and Regulation of c-Myb Target Genes in MCF-7 Cells. BMC Cancer. 11:30.

• Liu F, Zhao X, Perna F, Wang L, Koppikar P, Abdel-Wahab O, Harr MW, Levine RL, Xu H, Tefferi A, Deblasio A, Hatlen M, Menendez S, Nimer SD (2011). JAK2V617F-mediated phosphorylation of PRMT5 downregulates its methyltransferase activity and promotes myeloproliferation. Cancer Cell. 19(2):283-94.

• Wang L, Gural A, Sun XJ, Zhao X, Perna F, Huang G, Hatlen MA, Vu L, Liu F, Xu H, Asai T, Xu H, Deblasio T, Menendez S, Voza F, Jiang Y, Cole PA, Zhang J, Melnick A, Roeder RG, Nimer SD (2011). The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation. Science 333(6043):765-9. • Dawson MA, Bannister AJ, Saunders L, Wahab OA, Liu F, Nimer SD, Levine RL, Göttgens B, Kouzarides T, Green AR (2011). Nuclear JAK2. Blood 18(26):6987-8.

Awards and Honors:

Scholarships for Honor Students, Department of Stomatology, Tianjin University of Medicine, Tianjin, P.R. China, 08/1987—08/1992

First-year Outstanding Student, Biomedical Sciences Graduate Program, Health Sciences Center, University of New Mexico, Albuquerque, 2001.

Clinical Scholar Training Fellowship, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, 2009.

ASH Travel Award, 52nd Annual Meeting of the American Society of Hematology in Orlando, FL, December 4-7, 2010